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Introduction of Kjeldahl Digestion Method for Nitrogen Stable Isotope Analysis (δ15N-NO3 and δ15NNH4) and Case Study for Tracing Nitrogen Source (Kjeldahl 증류법을 활용한 질산성-질소 및 암모니아성-질소 안정동위원소비 분석 및 질소오염원 추적 사례 연구)

  • Kim, Min-Seob;Park, Tae-Jin;Yoon, Suk-Hee;Lim, Bo-La;Shin, Kyung-Hoon;Kwon, Oh-Sang;Lee, Won-Seok
    • Korean Journal of Ecology and Environment
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    • v.48 no.3
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    • pp.147-152
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    • 2015
  • Nitrogen (N) loading from domestic, agricultural and industrial sources can lead to excessive growth of macrophytes or phytoplankton in aquatic environment. Many studies have used nitrogen stable isotope ratios to identify anthropogenic nitrogen in aquatic systems as a useful method for studying nitrogen cycle. In this study to evaluate the precision and accuracy of Kjeldahl processes, two reference materials (IAEA-NO-3, N-1) were analyzed repeatedly. Measured the ${\delta}^{15}N-NO_3$ and ${\delta}^{15}N-NH_4$ values of IAEA-NO-3 and IAEA-N-1 were $4.7{\pm}0.2$‰ and $0.4{\pm}0.3$‰, respectively, which are within recommended values of analytical uncertainties. Also, we investigated spatial patterns of ${\delta}^{15}N-NO_3$ and ${\delta}^{15}N-NH_4$ in effluent plumes from a waste water treatment plant in Han River, Korea. ${\delta}^{15}N-NO_3$ and ${\delta}^{15}N-NH_4$ values are enriched at downstream areas of water treatment plant suggesting that dissolved nitrogen in effluent plumes should be one of the main N sources in those areas. The current study clarifies the reliability of Kjeldahl analytical method and the usefulness of stable isotopic techniques to trace the contamination source of dissolved nitrogen such as nitrate and ammonia.

Effects of Fermented feed with Agricultural by-products on the Growth Performance and Nutrients Utilization in Fattening Korean Black Goats (농산부산물 발효사료 급여가 비육흑염소의 생산성 및 영양소 이용율에 미치는 영향)

  • Choi, Sun-Ho;HwangBo, Soon;Kim, Sang-Woo;Cho, Young-Moo;Yoo, Yeoung-Hee;Kim, Tae-Il;Kim, Maeong-Joung;Lee, Sang-Min;Choi, Chang-Weon;Seo, Byeong-Bu;Jo, Ik-Hwan;Hong, Seonng-Gu
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.32 no.1
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    • pp.49-58
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    • 2012
  • This study was conducted to investigate the feeding fermented agro-industrial by-products on estimating the productivity and nutrient utilization of fattening black goats. Four experimental groups were set up with each of 6 animals for a 30 days feeding trial on growth performance and 12 black goats on nutrient digestibility were allocated to 4 groups of 3 animals each by latin-square design, control (offered concentrate and rice straw) and 3 treatment groups (offered fermentation feed and rice straw). Average daily gain (ADG) showed a significant high in control group, T3 was lower than other groups (p<0.05). DM and OM intake (kg/head) were 718.8, 680.9 g and 634.2~699.2 g, 602.8~660.4 g for control and treatment groups, respectively. As increasing of rice bran content was decreased feed intake. Digestibility of DM and OM for control group were significantly higher than treatment groups (p<0.05), and T3 treatment group with 30% rice bran was the lowest among treatment groups. Nitrogen retention was significantly higher in control and T1 groups. Thus, these results showed that productivity and nutrient utilization were similar between fermentation feeds with agricultural by-products and commercial feeds. However, this study was suggested that it must contain less than 5.0% crude fat because of feed efficient was decreased by increasing of rice bran content.

Studies on the Desertification Combating and Sand Industry Development(III) - Revegetation and Soil Conservation Technology in Desertification-affected Sandy Land - (사막화방지(沙漠化防止) 및 방사기술개발(防沙技術開發)에 관한 연구(硏究)(III) - 중국(中國)의 황막사지(荒漠沙地) 녹화기술분석(綠化技術分析) -)

  • Woo, Bo-Myeong;Lee, Kyung-Joon;Choi, Hyung-Tae;Lee, Sang-Ho;Park, Joo-Won;Wang, Lixian;Zhang, Kebin;Sun, Baoping
    • Journal of Korean Society of Forest Science
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    • v.90 no.1
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    • pp.90-104
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    • 2001
  • This study is aimed to analyze and to evaluate the revegetation and soil conservation technology in desertification-affected sandy land, resulting from the project of "Studies on the desertification combating and sand industry development". Main native plants for combating desertification : The general characteristics of vegetation distribution in desertified regions are partially concentrated vegetation distribution types including the a) desert plants in low zone of desert or sanddune of depressed basin, b) salt-resistant plants around saline lakes, c) grouped vegetation with Poplar and Chinese Tamarix of freshwater-lakes, saline-lakes and river-banks, d) gobi vegetation of gravel desert and e) grassland and oasis-woods around the alluvial fan of rivers, etc. Generally, Tamarix ehinensis Lour., Haloxylon ammodendron Bunge., Calligonum spp., Populus euphratica Oliver., Elaeagnus angustifolia L., Ulmus pumila L., Salix spp., Hedysarum spp., Caragana spp., Xanthoceras sorbifolia Bunge., Nitraria tangutorum Bobr., Lespedeza bicolor, Alhagi sparsifolia Shap., Capparis spinosa L., Artemisia arenaria DC., etc. are widely distributed in desertified regions. It is necessary for conducting research in the native plants in desertified regions. Analysis of intensive revegetation technology system for combating desertification : In the wind erosion region, the experimental research projects of rational farming systems (regional planning, shelterbelts system, protection system of oasis, establishment of irrigation-channel networks and management technology of enormous farmlands, etc.), rational utilization technology of plant resources (fuelwood, medicinal plants, grazing and grassland management, etc.), utilization technology of water resources (management and planning of watershed, construction of channel and technology of water saving and irrigation, etc.), establishment of sheltetbelts, control of population increase and increased production technology of agricultural forest, fuelwood and feed, etc. are preponderantly being promoted. And in water erosion region, the experimental research projects of development of rational utilization technology of land and vegetation, engineering technology and protection technology of crops, etc. are being promoted in priority. And also, the experimental researches on the methods of utilization of water (irrigation, drainage, washing and rice cultivation, etc.), agricultural methods (reclamation of land, agronomy, fertilization, seeding, crop rotation, mixed-cultivation and soil dressing works, etc.) and biological methods (cultivation of salt-resistant crops and green manure and tree plantation, etc.) for improvement of saline soil and alkaline soil in desertified-lands are actively being promoted. And the international cooperations on the revegetation technology development projects of desertified-lands are sincerely being required.

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Choroidal Thickness in Thyroid-associated Ophthalmopathy between Normal Tension Glaucoma Using Optical Coherence Tomography (스펙트럼영역 빛간섭단층촬영으로 측정한 갑상선 안병증 환자와 녹내장환자의 맥락막 두께 분석)

  • Lee, Bo Young;La, Tae Yoon;Choi, Jin A
    • Journal of The Korean Ophthalmological Society
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    • v.58 no.8
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    • pp.960-967
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    • 2017
  • Purpose: To compare the macular choroidal thickness in patients with thyroid-associated ophthalmopathy (TAO) with those with normal tension glaucoma (NTG). Methods: A total of 70 normal eyes, 74 eyes with TAO and 60 eyes with NTG were enrolled in this study. All patients underwent spectral-domain optical coherence tomography (SD-OCT) (Cirrus HD-OCT, Carl Zeiss Meditec Inc., Dublin, CA, USA). Macular choroidal thickness was assessed using enhanced depth imaging. The average macular choroidal thickness was defined as the average value of three measurements: at the fovea and at the points located 1.5 mm in the nasal and temporal directions from the fovea. Generalized estimating equations were used to uncover factors affecting the average macular choroidal thickness. Results: The average, superior and inferior quadrant retinal nerve fiber layer thicknesses were significantly thinner in the NTG group compared with the TAO and control groups (p < 0.001). The average macular choroidal thickness of the TAO group, NTG group and controls was $281.01{\pm}60.06{\mu}m$, $241.66{\pm}55.00{\mu}m$ and $252.07{\pm}55.05{\mu}m$, respectively, which were significantly different (p = 0.013). The subfoveal, nasal and temporal side choroidal thicknesses were significantly thinner in the NTG group compared with the TAO group (p = 0.014, 0.012 and 0.034, respectively). Subjects with TAO were associated with a thicker average macular choroidal thickness compared with the NTG group after adjusting for age, sex, spherical equivalent and intraocular pressure (${\beta}=32.61$, p = 0.017). Conclusions: Macular choroidal thickness was significantly thicker in patients with TAO compared with those with NTG. Further evaluation is required to determine if a thick choroid in subjects with TAO has any role in glaucomatous optic neuropathy.

Effective Combination of Resistance Genes against Rice Bacterial Blight Pathogen (벼흰잎마름병 저항성 증진을 위한 유전자 조합)

  • Kim, Ki-Young;Shin, Mun-Sik;Kim, Woo-Jae;Mo, Young-Jun;Nam, Jeong-Kwon;Noh, Tae-Hwan;Kim, Bo-Kyeong;Ko, Jae-Kwon
    • Korean Journal of Breeding Science
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    • v.41 no.3
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    • pp.244-251
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    • 2009
  • This study was carried out to identify useful single gene and gene combination resistant to K1, K2, K3 and 24 bacterial blight(BB) isolates (including K3a, HB01009) breaking down Xa3 gene. Xa3, Xa4, xa5 and Xa7 genes were resistant to K1, K2, K3 of bacterial blight pathogen. Against 24 BB isolates breaking down Xa3 gene, Xa1, Xa2, xa8, Xa10, Xa11, xa13 genes were susceptible, whereas Xa4 gene was moderately resistant and xa5 and Xa21 genes were resistant. IRBB7 having Xa7 gene showed resistance responding to 24 BB isolates, whereas IRBB107 carrying Xa7 gene was susceptible to 10 BB isolates and moderately resistant to 14 BB isolates. Near-isogenic lines (NILs) of Toyonishiki and IR24, both possessing Xa7 gene, showed different resistance response against 24 BB isolates according to genetic background. Xa3+xa5, Xa4+xa5, Xa4+xa13, Xa4+Xa21, xa5+xa13, xa5+Xa21, xa13+Xa21, Xa4+xa5+xa13, Xa4+xa5+Xa21, Xa4+xa13+Xa21, xa5+xa13+Xa21, and Xa4+xa5+xa13+Xa21 were resistant to K1, K2, K3 and 24 isolates breaking down Xa3 gene. When Xa3 and xa13 genes were combined with xa5, Xa4, Xa21, resistance response was enhanced compared with single gene lines containg only Xa3 or xa13. Similarly, when Xa4 gene was combined with xa5 and Xa21, resistance response was improved by the gene combination effect.

A New Early-Maturing Rice Intermediate parent with High-Quality, Good Taste, and Viviparous germination resistance, 'Jungmo 1012' (벼 조생 고품질 고식미 내수발아성 중간모본 '중모1012호')

  • Kim, Woo-Jae;Joung, Jin-Il;Kang, Jong-Rae;Park, Hyun-Su;Nam, Min-Hee;Kwak, Do-Yeon;Kim, Dae-Sik;Kim, Young-Doo;Kim, Jeong-Il;Song, You-Chun;Yeo, Un-Sang;Shin, Mun-Sik;Lee, Jong-Hee;Park, No-Bong;Cho, Jun-Hyeon;Kang, Hyeon-Jung;Kim, Bo-Kyeong;Ha, Ki-Yong;Nam, Jeong-Kwon;Baek, Man-Kee;Kim, Ki-Young;Ko, Jong-Cheol;Shin, Woon-Chul;Mo, Young-Jun;Park, Seong-Tae;Kang, Hang-Won;Ko, Jae-Kwon
    • Korean Journal of Breeding Science
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    • v.43 no.6
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    • pp.549-553
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    • 2011
  • 'Jungmo 1012', a new japonica rice intermediate parent developed from a cross between 'Sambaegbyeo' having a good eating-quality and lodging resistance, and 'Ou329', was developed by the rice breeding team of Sangju Substation, National Institute of Crop Science, Rural Development Administration in 2010. This variety has about 120 days growth duration from transplanting to harvesting in southern mid-mountainous area of Korea. It has 78 cm culm length and tolerance to lodging. In reaction to biotic and abiotic stresses, it shows strong resistance to blast and viviparous germination, but is susceptible to bacterial blight, virus diseases, and insect pests. The milled rice of 'Jungmo 1012' exhibit translucent, relatively clear non-glutinous endosperm and medium short grain. It has a little lower amylose content of 18.2%, and higher protein content of 6.3%, and good palatability of cooked rice compared with 'Odaebyeo'. The milled rice yield performance of this variety is about 5.55 MT/ha in local adaptability test for three years. 'Jungmo 1012' will be adaptable to southern mid-mountainous of South Korea.

A Mid-late Maturing Rice Cultivar with High-Quality and Bacterial Blight Resistance "Jinbaek" (벼 중만생 고품질 흰잎마름병 신균계(K3a) 저항성 품종 "진백")

  • Kim, Ki-Young;Shin, Mun-Sik;Kim, Bo-Kyeong;Ko, Jae-Kwon;Noh, Tae-Hwan;Ha, Ki-Yong;Ko, Jong-Cheol;Kim, Woo-Jae;Nam, Jeong-Kwon;Baek, Man-Gee;Noh, Gwang-Il;Park, Hyun-Su;Baek, So-Hyeon;Shin, Woon-Chul;Mo, Young-Jun;Choung, Jin-Il;Kim, Young-Doo;Kang, Hyun-Jung;Kim, Chung-Kon;Hwang, Hung-Goo;Kim, Je-Kyu
    • Korean Journal of Breeding Science
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    • v.41 no.3
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    • pp.314-318
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    • 2009
  • A new rice cultivar "Jinbaek" carrying Xa3 and xa5 was derived from the cross between 'HR15204-38-3' with xa5 gene resistant to bacterial blight K1, K2, K3 and K3a, and F1 plant derived from the cross between Junam and Sindongjin with Xa3 gene. "Jinbaek" has approximately 125 days of growth duration from transplanting to harvesting in the west-southern coastal and Honam plain of Korea. Culm length of "Jinbaek" is 71 cm. In reaction to biotic stresses, it shows moderate resistance to blast, and wide spectrum resistance to bacterial blight pathogen, K1, K2, K3, and K3a but susceptible to rice stripe virus and blast. The milled rice of "Jinbaek" exhibits translucent, relatively clear non-glutinous endosperm and midium short grain. It has lower amylose content (18.8%) and protein content (6.2%) compared with Nampyeong. The milled rice yield of this cultivar was 5.30 MT/ha in local adaptability test of three years from 2006 to 2008. This cultivar would be adaptable to the bacterial blight-prone area in the south-western coast and Honam plain of Korea.

Comparison of Growth Characteristics of Creeping Bentgrass(Agrostis palustris Huds.) Cultivars in Summer (하절기 크리핑 벤트그래스의 품종별 특성비교)

  • Tae, Hyun-Sook;Lee, Hyung-Seok;An, Kil-Man;Kim, Jong-Bo
    • Asian Journal of Turfgrass Science
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    • v.20 no.2
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    • pp.147-156
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    • 2006
  • This study was initiated to evaluate the growth characteristics of seven creeping bentgrass cultivars in summer, 'Penncross' showed the worst visual quality, whereas 'Penn A-4' and 'Crenshaw' the best quality. 'Putter', which was maintained a fair quality during the test period, was regarded as a good cultivar because of no significant variation in summer as compared to the other caltivars. 'Crenshaw',' L-93' and 'Penn A-4' were greater in chlorophyll content and 'Penncross' lowest during the summer. Also, 'SR1020' had a low content of chlorophyll. 'Putter' greatly increased in chlorophyll content after fertilization. The highest shoot density($19.3/cm^2$) was found with 'L-93' in early August, followed by 'Crenshaw', 'Penn A-4', 'Putter', 'Dominant', and 'SR1020' in that order. However, 'Penncross' was lowest($15.7/cm^2$). As for a root length, 'L-93' was longest, being over an average 5.5cm. 'Penn A-4' and 'Putter' also showed good result in root growth. However, the root length considerably decreased with 'SR1020', 'Penncross' and 'Dominant' in summer. Brown patch was a serious disease for the most cultivars, except 'Penncross'. 'Dominant' had the most serious damage. 'Putter', 'L-93', 'Crenshaw', 'SR1020', and 'Penn A-4' were also greater in damage over the others. In regards of algae occurrence in summer, 'Penn A-4' had the least damage, while 'Dominant' the greatest. In conclusion, 'Crenshaw', 'Penn A-4' and 'L-93' were the best cultivars in terms of summer growth. Conversely, 'Penncross' was the poorest one. However, this study was conducted under the conditions of one-year old green. Accordingly, in-depth experiment should be done over several years to elucidate the characteristics of growth for the wide range of creeping bentgrass cultivars during the summer.

Analysis of Lumbar Herniated Intervertebral Disc Patients' Healthcare Utilization of Western-Korean Collaborative Treatment: Using Health Insurance Review & Assessment Service's Patients Sample Data (요추 추간판 탈출증 환자의 의·한의 협진 의료이용 현황 분석: 건강보험심사평가원 환자표본 데이터를 이용하여)

  • Ko, Jun-Hyuk;Yu, Ji-Woong;Seo, Sang-Woo;Seo, Joon-Won;Kang, Jun-Hyuk;Kim, Tae-Oh;Cho, Whi-Sung;Seo, Yeon-Ho;Ahn, Jong-Hyun;Lee, Woo-Joo;Kim, Bo-Hyung;Choi, Man-Khu;Kim, Sung-Bum;Kim, Hyung-Suk;Kim, Koh-Woon;Cho, Jae-Heung;Song, Mi-Yeon;Chung, Won-Seok
    • Journal of Korean Medicine Rehabilitation
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    • v.31 no.4
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    • pp.105-116
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    • 2021
  • Objectives Lumbar herniated intervertebral disc (L-HIVD) is common disease in which Western-Korean collaborative treatment is performed in Korea. This study aimed to analyze Western-Korean collaborative treatment utilization of Korean patients with L-HIVD using Health Insurance Review & Assessment Service's Patients Sample Data. Methods This study used the Health Insurance Review & Assessment Service-National Patient Sample (HIRA-NPS) in 2018. Claim data of L-HIVD patients were extracted. The claim data were rebuilt with the operational concept of 'episode of care' and divided into Korean medicine episode group (KM), Western medicine episode group (WM) and collaborative treatment episode group (CT). General characteristics, medical expenses and healthcare utilization were analyzed. In addition, the difference of average visit day and average medical expenses between non-collaborative group (KM plus WM) and CT were analyzed by the propensity score matching method. Results A Total of 64,333 patients and 365,745 claims were extracted. The number of episodes of WM, KM and CT was 69,383 (92.97%), 3,903 (5.23%), and 1,341 (1.80%) respectively. The frequency of collaborative treatment episode was higher in women and the age of 50s. The most frequently described treatment in CT was acupuncture therapy. As a result of the propensity score matching, the number of visit days and medical expenses in the collaborative treatment group was higher than in the non-collaborative group. Conclusions The analysis of healthcare utilization of Korean-Western collaborative treatment may be used as basic data for establishing medical policies and systematic collaborative treatment model in the future.

A New Medium-Maturing, "Gangbaek" with Resistance to Bacterial Blight (벼 중생 흰잎마름병 저항성 신품종 "강백")

  • Kim, Ki Young;Shin, Mun Sik;Kim, Woo Jae;Ko, Jong Cheol;Baek, Man Gee;Ha, Ki Yong;Kim, Bo Kyeong;Ko, Jae Kwon;Nam, Jeong Kwon;Noh, Gwang Il;Park, Hyun Su;Noh, Tae Hwan;Noh, Jae-Hwan;Cheong, Jin Il;Kim, Young Doo;Mo, Young Jun;Kim, Chung Kon
    • Korean Journal of Breeding Science
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    • v.40 no.4
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    • pp.443-446
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    • 2008
  • 'Gangbaek' is a japonica rice variety developed and registered by the rice breeding team of Department of Rice and Winter Cereal Crop, NICS, RDA in 2006. 'Gangbaek' was derived from a cross between 'Suweon345' with good grain quality and 'DV85' resistant to bacterial blight, $K_{3a}$. $F_1$ plants were grown in the greenhouse in winter of 1992/1993 and backcrossed with 'Suweon345' as the recurrent parent. Plants resistant to $K_{3a}$ race of bacterial blight (BB) were selected from $BC_1F_1$ to $BC_4F_1$ and used as parents in the backcrossing processes. This variety has about 120 days growth duration from transplanting to harvesting in west-southern coast and Honam plain of Korea. It is about 69 cm in culm length and tolerance to lodging. In reaction to biotic and abiotic stresses, it shows moderately resistance to blast, and resistance to bacterial blight pathogen, $K_1$, $K_2$, $K_3$ and $K_{3a}$, but susceptible to other major diseases and insect pests. The milled rice of 'Gangbaek' exhibits translucent, relatively clear non-glutinous endosperm and midium short grain. It has lower amylose content of 18.6% and protein content of 6.4% compared with 'Nampyeongbyeo'. The milled rice yield performance of this variety is about 5.28 MT/ha in local adaptability test for three years. This cultivar would be adaptable to the bacterial blight-prone area in the south-western coastal and Honam plain of Korea.