• Title/Summary/Keyword: TS

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Partial Characterization of Bacillus thuringiensis var. kurstaki Temperature-sensitive Mutants (Bacillus thringiensis var. kurstaki 감온성 돌연변이주의 일부특성)

  • 김영권;유관희;이형환;이호원
    • Korean Journal of Microbiology
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    • v.23 no.3
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    • pp.203-208
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    • 1985
  • Partial characterization of B. thuringiensis var. kurstaki 3ab temperature-sensitive mutants was carried out through biochemical analyses, utilization tests of carbohydrate sources, antobiotic resistant test, hemolytic reaction test, growth measurement of Fructus gardenia sxtrant medium and toxicity test against mice. Six ts mutants, ts-U154, ts-U601, ts-U602, ts-U603, tsU-604, and ts-U788 could not produce urease, ts-U603 lost its motility, ts-U154 could not use salicin and cellobiose and ts-U603 not ribose. All ts mutants except ts-U154 and wild type strain were resistant to cephalothin, ampicillin, and penicillin. but ts-U154 was sensitive to the three. Four mutants, ts-U21, ts-U74, ts0U131 and ts-U154 did not form pigment colonies on the F. gardenia medium. All the mutants and wild type strain showed hemolysis reaction on the blood agar. The B. thuringiensis and mutants were not toxic to mice.

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Isolation and Identification of Probiotic Bacillus strain Forming Amine Oxidase from Traditional Fermented Soybean Paste (재래식 된장으로부터 아민 산화 효소를 생산하는 프로바이오틱 바실러스균의 분리 동정)

  • Lim, Eun-Seo
    • Journal of the Korean Applied Science and Technology
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    • v.37 no.6
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    • pp.1535-1544
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    • 2020
  • The primary objective of this study was to isolate and identify amine oxidase-producing probiotic Bacillus strains from traditional fermented soybean paste. Biogenic amines (BA)-forming bacteria isolated from the samples were identified as Bacillus sp. TS09, Bacillus licheniformis TS17, Bacillus subtilis TS19, Bacillus cereus TS23, Bacillus sp. TS30, Bacillus megaterium TS31, B. subtilis TS44, Bacillus coagulans TS46 and Bacillus amyloliquefaciens TS59. Meanwhile, B. subtilis TS04 and TS50 isolated from the same samples exhibited good probiotic properties, including the tolerance to artificial gastric juice and bile salts, the adhesion to intestinal epithelial cells, and the production of bacteriocin(s) active against BA-forming bacteria (Bacillus sp. TS30 and B. subtilis TS44). In addition, the amine oxidase produced by B. subtilis TS04 and TS50 significantly decreased the formation of BA, especially cadaverine, putrescine, and tyramine, therefore, these strains could be considered good potential probiotic candidates to prevent or reduce BA accumulation in food products.

Accuracy Analysis of Target TS Surveying and Non-target TS Surveying for Building Registration of Cadastral Map (지적도 건물등록을 위한 타켓 TS 측량과 무타켓 TS 측량의 정확도 분석)

  • Hong, Sung-Eon
    • Spatial Information Research
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    • v.15 no.2
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    • pp.123-134
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    • 2007
  • The building boundary should be registered in the cadastral map for the protection of property using the expansion of the registration items in the cadastral record and the construction of 3D-cadastral information system. In this study, the efficiency of registering the building boundary was suggested by non-target TS survey through the comparison between target TS and non-target TS survey about an accuracy and efficiency. At the result of this research, the RMSE of target TS and non-target TS survey is the X; ${\pm}0.056m$ and Y; ${\pm}0.043m$. Therefore, non-target TS survey shows the high accuracy. Also, the non-target TS survey is more efficient cost on time and personnel than target TS.

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The Relation between Microsatellite Instability and the Thymidylate Synthase Expression in Gastric Cancer (위암에서 Microsatellite Instability와 Thymidylate Synthase의 상관관계)

  • Ko, Hyun-Seok;Ahn, Chang-Wook;Kang, Hae-Youn;Kim, Kwang-Il;Hong, Sung-Pyo;Ahn, Dae-Ho
    • Journal of Gastric Cancer
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    • v.8 no.4
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    • pp.169-175
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    • 2008
  • Purpose: The main target of 5-fluorouracil (5-FU) is thymidylate synthase (TS). A high TS expression has been identified as promoting resistance to 5-FU. For colorectal cancers, the response to 5-FU based adjuvant chemotherapy is different according to the microsatellite instability (MSI) status. The reports on the relationship between MSI and the TS expression in colorectal cancer have been inconsistent. No data is available for gastric cancer regarding the relationship between MSI and the TS expression. Therefore, we studied the relationship between MSI and the TS expression in gastric cancer. Materials and Methods: Ninety-nine consecutive patients who underwent radical gastrectomy for gastric cancer from January 2004 to May 2006 at Bundang CHA hospital were studied. MSI was assessed for five markers (BAT25, BAT26, D2S123, D5S346, and D17S250) by PCR and with using an ABI prism 3100 Genetic analyzer. The TS expression was analyzed by immunohistochemistry with using mouse anti-thymidylate synthase monoclonal antibody to the TS 106 clone. Results: Out of the ninety-nine patients, MSS/MSI-L was detected in 92 (92.1%) cases and 7 cases (7.1%) were MSI-H. A negative TS expression was found in 46 (46.5%) cases, a low TS expression was found in 33 (33.3%) and a high TS expression was found in 20 (20.2%). Out of 92 MSS/MSI-L patients, the number of patients with negative, low and high TS expressions were 46 (50%), 30 (32.6%) and 16 (17.4%), respectively. Out of the 7 MSI-H patients, the number of patients with negative, low and high TS expressions were 0 (0%), 3 (42.9%) and 4 (57.1%), respectively. The relationship between MSI-H and a high TS expression was statistically significant. Conclusion: Gastric cancer with MSI-H showed higher levels of a TS expression compared to the gastric cancer with MSS/MSI-L.

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Stochastic Stabilization of TS Fuzzy System with Markovian Input Delay (마코프 입력 지연을 갖는 TS 퍼지 시스템의 확률전 안정화)

  • 이호재;주영훈;이상윤;박진배
    • Journal of the Korean Institute of Intelligent Systems
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    • v.11 no.6
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    • pp.459-464
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    • 2001
  • This paper discusses a stochastic stabilization of Takagi-Sugeno(TS) fuzzy system with Markovian input delay. The finite Markovian process is adopted to model the input delary of the overall control system. It is assumed that the zero and hold devices are used for control input. The continuous-time TS fuzzy system with the Markovian input delay is discretized for easy handling delay, according, the discretized TS fuzzy system is represented by a discrete-time TS fuzzy system with jumping parameters. The stochastic stabilizibility of the jump TS fuzzy system is derived and formulated in terms of linear matrix inequalities (LNIS)

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Efficacy and Safety Profile of TS-1 or TS-1/CDDP in Patients with Advanced Gastric Cancer (진행성 위암환자에 있어 TS-1 또는 TS-1/CDDP의 항암효과 및 안정성)

  • Ha,, Tae-Kyung;Kwon, Sung-Joon
    • Journal of Gastric Cancer
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    • v.6 no.3
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    • pp.139-145
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    • 2006
  • Purpose: Although several chemotherapy regimens used against advanced gastric cancer (AGC) have been studied extensively in an attempt to further improve the prognosis of patients, to date, no standard chemo-therapeutic regimens have been established. The aim of this study was to determine the anti-tumor efficacy and safety of TS-1 or TS-1 plus cisplatin (CDDP). Material and Methods: We treated 78 patients with AGC either with $80\;mg/m^{2}$ of TS-1 for 28 days, which was followed by a 2-week rest, or with $80\;mg/m^{2}$ of TS-1 for 21 days and $80\;mg/m^{2}$ of CDDP on day 8 every 5 weeks. Results: Tumor response rates in the neoadjuvant chemotherapy group and in the recurrent or post-palliative surgery group were 87.5% and 32.4%, respectively, and they were 28.6% and 48.4%, respectively, in the TS-1 group and the TS-1 plus CDDP group. The survival rates in the recurrent and the post-palliative surgery group were significantly different according to the degree of tumor response (P=0.0016), but the one-year survival rates according to the kinds of regimens (TS-1 or TS-1/CDDP group) were not significantly different. The incidences of grade 3 or 4 adverse effects in the TS-1 and the TS-1/CDDP groups were 14.3% and 36.8%, respectively. Conclusion: The anti-tumor efficacy and safety of TS-1 and TS-1 plus CDDP in Korean patients with AGC seemed to be high with modest adverse effects, thus suggesting the possible use of this regimen as a standard chemotherapy for gastric cancer.

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Rapid detection of Theileria sergenti by polymerase chain reaction (중합효소연쇄반응을 이용한 Theileria sergenti의 신속한 검출)

  • 최은진;강승원
    • Parasites, Hosts and Diseases
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    • v.35 no.2
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    • pp.111-118
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    • 1997
  • Four separate pairs of oligonucleotide primers within the coding region in a T sergenti 33-kDa surface protein gene were selected to detect T. sergenti by PCR. The specificity of PCR-amplified DNA was examined by digestion with restriction enzyme 3nd Southern blot hybridization using T. sergenti p33 DNA probe. PCR appears to be specific for T. sergenti, without detectable signals from uninfected erythrocytes, uninfected bovine leukocytes and other hemoparasites, including A. morginnle and 3. ouata. Although 46 of 71 specimens (64.8%) from grazing cattle were microscopically positive. PCR in this study showed that 64 specimens (88.7%) were positive. Therefore, PCR proves a useful diagnostic tool for detecting T sergenti-infected cattle. In addition, it is also revealed that PCR was significantly more sensitive than traditional microscopic examination using Giemsa's stain.

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Immunological Variations of Flagella Antigens in Bacillus thuringiensis serovar kurstaki Temperature-sensitive Mutants (Bacillus thuringiensis serovar kurstaki 돌연변이 균주의 편모항원의 변이성)

  • 이형환;이영주;이희무
    • Microbiology and Biotechnology Letters
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    • v.17 no.1
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    • pp.81-83
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    • 1989
  • The flagella antigenic variation of nine Bacillus thuringiensis serovar kurstaki temperature-sensitive mutants grown at the permissive temperature (3$0^{\circ}C$) was detected by a serological agglutination between H-antigen and antiserum. The flagella antigens were injected to rabbits to prepared their antisera, and then their homologous and heterologous titers of the antisera were measured. The homologous titers were ranged from 1:6,400 to 1:12,800, but the heterologous titers were very low. The H-antigen of the wild type strain was not agglutinated to 4 heterologous antisera, ts-U23 not to 7, ts-U3l not 5, ts-U32 not to 4, ts-U33 not to 7, ts-U7l not to 4, ts-U73 not to 6, ts-U74 not to 6, ts-U91 not to 4 and ts-U603 not to 4 antisera.

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In situ side-aspect target strength of Japanese anchovy (Engraulis japonicus) in northwestern Pacific Ocean (북서 태평양 멸치(Japanese anchovy)에 대한 측면 음향 반사강도 특성)

  • Lee, Hyung-Been;Kang, Don-Hyug
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.46 no.3
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    • pp.248-256
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    • 2010
  • Acoustic side-aspect target strength (TS) of living Japanese anchovy (Engraulis japonicus) was measured at 120kHz during in situ experiments. The data were collected by lowering and horizontally projecting the splitbeam transducer into the anchovy school. For analysis and interpretation of the side-aspect TS data, acoustic theoretical model, based on the fish morphology, and dorsal-aspect TS data were used. Total length of the anchovy ranged from 6.6 to 12.8cm (mean length 9.3cm). The side-aspect TS distributed between -40 and -55dB, has an obvious length dependency. The mean side-aspect TS of the anchovy was -47.8dB, and the TS was about 2dB higher than mean TS generated from dorsal-aspect measurements. With reference to maximum TS, the results of the side-aspect TS were distributed within the range of the theoretical and dorsal-aspect TS. Apparently these tendency indicates that side-aspect TS measured from the study is useful data. These in situ measurements of side-aspect TS can be applied to improve acoustic detection and estimates of the anchovy, and is necessary to measure with a various frequency and length for making enhance data.

An Overhead Comparison of MMT and MPEG-2 TS in Broadcast Services (방송 서비스에서 MMT와 MPEG-2 TS의 오버헤드 비교)

  • Park, MinKyu;Kim, Yong Han
    • Journal of Broadcast Engineering
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    • v.21 no.3
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    • pp.436-449
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    • 2016
  • This paper compares the transport overhead of MMT (MPEG Media Transport) with that of MPEG-2 TS (Transport Stream). MPEG-2 TS is globally used in multiplexing compressed audio and video data in digital broadcast industry, including areas of DTV (Digital Television), IPTV (Internet Protocol Television), and DMB (Digital Multimedia Broadcasting). It was the early 1990s when MPEG-2 TS standard was established. After more than two decades of years since its first establishment, many parts of MPEG-2 TS turned out to be inappropriate to today's broadcast and communication environment. Given the situations, in 2014 MPEG (ISO/IEC JTC 1 SC 29/WG 11) standardized MMT as the next-generation multimedia transport standard hopefully that can replace MPEG-2 TS. In this paper, with assumptions of broadcast service scenarios we applied both MMT and MPEG-2 TS to each scenario and we calculated their transport overheads. We used a software program that counts the transport overhead, which was developed in our laboratory for this paper. And we conducted a comparative analysis based on the calculated result of transport overhead.