• 미생물학회지
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• 제38권4호
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• pp.247-253
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• 2002

환경오염원으로서 폭약 2,4,6-trinitrotoluene (TNT)에 대한 TNT 분해세균 Stenotrophomonas sp. OK-5의 세포반응에 대하여 조사하였다. 아치사조건의 TNT농도와 노출시간에 따른 균주 OK-5의 생존율을 분석한 결과, 이 세균의 생존율은 스트레스 충격 단백질의 생성과 비례하였다. 총세포 지방산 조성분석에서 균주 OK-5는 tryp-ticase soy agar에서 자랄 때보다 TNT 배지에서 자랄 때 여러 가지 종류의 지방산이 생성되거나 사라지는 것이 밝혀졌다. 주사전자현미경하에서 TNT에 노출된 세포는 쭈글쭈글하고 불규칙적인 간상형으로 나타났다. Anti-DnaK와 anti-GroEL을 이용하여 SDS-PAGE와 Western blot을 통한 분석으로 균주 OK-5는 70 kDa DanK와 60 kDa GroEL을 포함하는 몇가지 스트레스충격단백질을 생성하는 것으로 밝혀졌다. TNT에 노출된 OK-5 배양에서 수용성 단백질 분획에 대하여 2-D PAGE를 실시하였으며, pH 3에서 pH 10의 범위에서 약 300여 개 spot들이 silver로 염색된 gel상에서 관찰되었다. 이들 가운데 TNT의 반응으로 현저하게 유도되고 발현된 10개의 spot들을 확인하였으며, 2개의 단백질, spot #1과 spot #10에 대한 내부아미노산 서열을 ESI-Q TOF로 분석한 결과, Xylella fastidiosa의 DnaK protein XF2340와 Mesorhizobium loti의 스트레스 유도단백질로 각각 밝혀졌다.

• 미생물학회지
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• 제38권2호
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• pp.67-73
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• 2002

폭약 2,4,6-trinitrotoluene (TNT)스트레스 조건하에서 토양세균 Pseudomonas sp. HK-6의 세포반응에 대하여 조사하였다. 다양한 농도의 TNT에 노출됨으로서 약 70-kDa DnaK와 60-kDa GroEL의 스트레스 충격단백질 (stress shock proteins, SSPs)이 단떠질이 유도되었다. 이들 SSPs의 존재는 SDS-PAGE과 anti-DnaK와 anti-GroEL monoclonal antibodies를 이용한 Western bolt을 통하여 확인되었다. SSPs은 0.5 mM TNT로 6-12 시간 처리된 세포에서 나타났으며, TNT에 노출 후8시간대 에서 최대의 단백질 유도가 관찰되었다. $30^{\circ}C$에서 $42^{\circ}C$로 열변환충격을 주었을 때의 SSPs는 TNT노출에서와 유사한 유도양상을 보여주었다. TNT에 노출된 Pseudomonas sp. HK-6세포에서 유도된 SSPs의 존재는 배양된 세포의 수용성 단백질 분획에 대하여 2-D PAGE를 통하여 확인되었다. Coomassie brilliant blue R25O로 염색된 젤로부터 pH 3-10 범위에서 약 450 개의 spots이 탐침되었으며, 이들 가운데 12 개의 spots이 TNT 스트레스에 대하여 현저하게 유도되었다. Gel상에서 가장 짙게 나타난 대표적 인 spot에 대한 N-말단 아미노산 서열을 분석한 결과, $^1XXAKDVKFGDSARKKML^17$로서, Pseudomonas putida의 GroEL의 N-말단 아미노산 염기서열인 $^1XXAKDVKFGDSARKKML^17$과 동일한 것으로 분석되었다.

• 한국지하수토양환경학회지:지하수토양환경
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• 제20권6호
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• pp.73-84
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• 2015

Nine plant species were selected through vegetation survey at three military shooting ranges at northern Gyeonggi Province. Plants were germinated in normal soil and three seedlings were transplanted to a bottom sealed pot containing sandy loam soils contaminated with either RDX (291 mg/kg) or TNT (207 mg/kg). Planted, blank (without plant), and control (without explosive compound) pots were grown in triplicate at a green house for 134 days. During cultivation, transplanted plants exhibited chlorosis and necrosis in flower and leaf by explosive toxicity and stress. Only three plants, Wild soybean, Amur silver grass, Reed canary grass, survived in TNT treated pot, while seven plant species except for field penny cress and jimson weed, thrived in RDX treated pot. Appreciable amount of TNT (61.6~241.2 mg/g-D.W.) was detected only in plant roots. Up to 763.3 mg/g-D.W. along with 4-amino-2,6-dinitrotoluene, an intermediate of TNT, accumulated in the root of wild soybean. In addition, azoxy compounds, abiotic intermediates of TNT, were detected in TNT treated soils. RDX absorbed average 1,839.95 mg/kg in shoot and 204.83 mg/kg in root. Most of TNT in plant was accumulated in underground part whereas RDX was localized in aerial part. Material balance calculation showed that more than 95% of the initial TNT was removed in the planted pots whereas only 60% was removed in the blank pot. The amount of RDX removed from soil was in the order of Amur Silver Grass (51%) > Chickweed (43%) > Evening primrose (38%). Based on the results of pot cultures, Amur silver grass and Reed canary grass are selected as tolerant remedial plants for explosive toxicity.

• 한국동물학회:학술대회논문집
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• 한국동물학회 2000년도 한국생물과학협회 학술발표대회
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• pp.234.1-234
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• 2000

No Abstract, See Full Text

• Coupled systems mechanics
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• 제8권4호
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• pp.315-338
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• 2019

The numerical investigations have been carried out on reinforced concrete slab against blast loading to demonstrate the accuracy and effectiveness of the finite element based numerical models using commercial package ABAQUS. The response of reinforced concrete slab have been studied against the influence of weight of TNT, standoff distance, boundary conditions, influence of air blast and surface blast. The results thus obtained from simulations were compared with the experiments available in literature. The inelastic behavior of concrete and steel reinforcement bar has been incorporated through concrete damage plasticity model and Johnson-cook models available in ABAQUS were presented. The predicted results through numerical simulations of the present study were found in close agreement with the experimental results. The damage mechanism and stress response of target were assessed based on the intensity of deformations, impulse velocity, von-Mises stresses and damage index in concrete. The results indicate that the standoff distance has great influence on the survivability of RC slab against blast loading. It is concluded that the velocity of impulse wave was found to be decreased from 17 to 11 m/s when the mass of TNT is reduced from 12 to 6 kg. It is observed that the maximum stress in the concrete was found to be in the range of 15 to $20N/mm^2$ and is almost constant for given charge weight. The slab with two short edge discontinuous end condition was found better and it may be utilised in designing important structures. Also it is observed that the deflection in slab by air blast was found decreased by 60% as compared to surface blast.

• 한국가스학회지
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• 제26권5호
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• pp.58-65
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• 2022

Gas explosion accidents could cause a catastrophe. we need specialized and systematic accident investigation techniques to shed light on the cause and prevent similar accidents. In this study, we had performed LPG explosion simulation using AUTODYN which is the commercial explosion program and predicted the damage characteristics of the structures by LNG explosive power. In the first step, we could get LPG's physical and chemical explosion properties by calculation using TNT equivalency method. And then, by applying TNT equivalency value about the explosion limit concentration of LPG on the 2D-AUTODYN simulation, we could get the explosion pressure wave profiles (explosion pressure, explosion velocity, etc.). In the last step, we performed LPG explosion simulation by applying to the explosion pressure wave profiles as the input data on the 3D-AUTODYN simulation. As a result, we had performed analyzing of the explosion characteristics of LPG in accordance with concentration through the 3D-AUTODYN simulation in terms of the explosion pressure behavior and structure destruction and damage behavior. The analyses showed that the generated stresses of the structures were lower than the compressive strengths in cases 1(two lane) and 2(four lane), while the generated stress in case 3(six lane) was 8.68e3 kPa, which exceeded the compressive strength of 5.89e3 kPa.

• Structural Engineering and Mechanics
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• 제74권6호
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• pp.737-745
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• 2020

Nowadays, composite plates are widely used as high-strength structures to fabricate a dynamic loading-resistant armours. In this study, the shock load is applied by an explosion of spherical TNT charge at a specified distance from the circular composite plate. The composite plate contains a two-layer ceramic-metal armour and a poly-methyl methacrylate (PMMA) target layer. The dynamic behavior of the composite armour has been investigated by measuring the transferred effective stress and maximum deflection into the target layer. For this purpose, the simulation of the blast loading upon the composite structure was performed by using the load-blast enhanced (LBE) procedure in Ls-Dyna software. The effect of main process parameters such as the thickness of layers, and scaled distance has been examined on the specific stiffness of the structure using response surface method. After validating the results by comparing with the experimental results, the optimal values for these parameters along with the regression equations for transferred effective stress and displacement to the target have been obtained. Finally, the optimal values of input parameters have been specified to achieve minimum transferred stress and displacement, simultaneously with reducing the weight of the structure.

• 화약ㆍ발파
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• 제38권4호
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• pp.1-15
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• 2020

국내의 지하공간 활용이 증가함에 따라 지하에 매설되어 있는 가스관과 같은 시설물에서의 폭발 사고가 꾸준히 발생하고 있다. 인구밀도가 높은 도심지에서는 개별의 폭발 사고가 복합적인 큰 사고로 확산될 가능성이 존재한다. 따라서, 도심지에서의 폭발이 지하구조물의 안정성에 미치는 영향을 평가하는 것이 필요하다. 본 연구에서는 터널과 인접한 곳에서의 폭발이 지하구조물의 안정성에 영향력을 미치는 요인들의 영향을 파악하기 위하여 폭발 조건과 암반의 물성을 포함한 8개 인자들에 대한 민감도 분석을 실시하였다. AUTODYN을 이용한 민감도 분석을 통해 각 인자들의 주영향과 교호작용효과를 분석하였다. 분석 결과, 폭발지점과 지하구조물 사이의 거리, 폭약량, 암석의 탄성계수가 터널 주변 응력성분에 큰 영향을 미치는 것으로 나타났다.

• Computers and Concrete
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• 제21권4호
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• pp.399-406
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• 2018

The present study focuses on the performance of basalt fiber reinforced concrete (BFRC) lining in tunnel situated in sandstone rock when subjected to internal blast loading. The blast analysis of the lined tunnel is carried out using the three-dimensional (3-D) nonlinear finite element (FE) method. The stress-strain response of the sandstone rock is simulated using a crushable plasticity model which can simulate the brittle behavior of rock and that of BFRC lining is analyzed using a damaged plasticity model for concrete capturing damage response. The strain rate dependent material properties of BFRC are collected from the literature and that of rock are taken from the authors' previous work using split Hopkinson pressure bar (SHPB). The constitutive model performance is validated through the FE simulation of SHPB test and the comparison of simulation results with the experimental data. Further, blast loading in the tunnel is simulated for 10 kg and 50 kg Trinitrotoluene (TNT) charge weights using the equivalent pressure-time curves obtained through hydrocode simulations. The analysis results are studied for the stress and displacement response of rock and tunnel lining. Blast performance of BFRC lining is compared with that of plain concrete (PC) and steel fiber reinforced concrete (SFRC) lining materials. It is observed that the BFRC lining exhibits almost 65% lesser displacement as compared to PC and 30% lesser displacement as compared to SFRC tunnel linings.

• Coupled systems mechanics
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• 제7권3호
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• pp.333-351
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• 2018

In the present paper, the behavior of the Karaj double curvature arch dam is studied focusing on the effects of structural nonlinearity on the responses of the dam body when an underwater explosion occurred in the reservoir medium. The explosive sources are located at different distances from the dam and the effects of the cavitation and the initial shock wave of the explosion are considered. Different amount of TNT are considered. Two different linear and nonlinear behavior are assumed in the analysis and the dam body is assumed with and without contraction joints. Radial, tangential and vertical displacements of the dam crest are obtained. Moreover, maximum and minimum principal stress distributions are plotted. Based on the results, the dam body responses are sensitive to the insertion of joints and constitutive model considered for the dam body.