• 한국식품영양과학회지
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• 제35권10호
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• pp.1456-1460
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• 2006

변질된 팥앙금에서 분리한 균주는 광학 및 전자현미경을 통하여 관찰한 결과 그람양성 간균으로 포자를 형성하고 형성된 집락은 원형으로 점질성의 물질을 생산하는 mucoid type이었으며, 표면은 smooth 하였고 광택을 나타내었다. 미생물 동정 시스템($MicroLog^{TM}$ System, Release 4)인 VITEK 2 COMPACT을 이용하여 분리된 균주의 46개의 생화학적 분석을 비교한 결과 팥앙금의 부패 원인균은 Bacillus subtilus로 동정 (97.0%)되었으며, 보다 더 정확한 동정을 위하여 부패균의 세포벽 지방산 조성을 분석하여 Midi sherlock시스템의 databank와 비교 분석시 그 유사성이 0.724로 분석되었다. Bacillus subtilus의 포자의 D-value는 $115^{\circ}C$에서의 4.85분이 었고, $121^{\circ}C$에서는 0.69분, $125^{\circ}C$에서는 0.48분이었으며, Z-value는 $9.71^{\circ}C$이었다. $45^{\circ}C$에서 수분활성도에 따른 분리균의 생육 상태를 관찰한 결과 수분활성도 0.92이하에서는 균이 증식되지 않았고, 0.94에서는 증식되는 것으로 관찰되었다.

• Journal of Periodontal and Implant Science
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• 제38권3호
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• pp.475-482
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• 2008

Purpose: To evaluate the safety and efficiency of bone regenerative abilities of silk fibroin nanomembrane(Nanoguide-S) Material and Methods: The objects were 38 patients who had large defect at extraction sockets caused by chronic periodontitis and silk fibroin nano matrix were used on experimental group(N=19) and PLA/PLGA matrix were used on control group(N=19). The width, height, and length by crown-apical direction(socket depth) of defects were measured with the occlusal plane as a reference plane, and tooth axis direction, perpendicular to tooth axis direction were measured on radiographs at 3 months pre-operative, 3 months post-operative. Result: Tissue response to silk fibroin nano matrix and Biomesh were clinically satisfactory and complications such as swelling, exudation, ulceration and vesicles were not found except the ordinary discomfort of operated portion. 3 months later, the width, height, and length by crown-apical direction (socket depth) of defects were clinically improved in both groups with no significant difference. 3 months later radiolucency of tooth axis direction and perpendicular to tooth axis direction were all increased in both groups with no significant difference. Conclusion: By these results biodegradadable silk fibroin nano matrix was efficient in GBR on alveolar bone resorption caused by periodontitis compared to Biomesh.

• 대한공간정보학회지
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• 제10권1호
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• pp.83-90
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• 2002

GPS 상대측위기법에 있어 고정점으로 이용되는 기준점에 과대오차가 내재되어 있다면, GPS의 우수한 성능에도 불구하고 신설점에 미치는 오차의 영향은 다양한 형태의 오차로 전파 될 수 있다. 따라서 본 연구에서는 신설점 선정에 기준점으로 이용될 삼각점들의 오차 분석을 위해 GPS관측을 2개의 세션으로 나누어 수행하고 기선해석과 망조정을 통해 TM 좌표를 구하고 다시 GPS관측이 이루어진 삼각망에 대해 각각의 기선을 EDM으로 관측하고 또한 현행성과를 수평거리로 환산하여 각각 거리를 비교 하였고 좌표분석은 EBM에 의해 관측된 기선을 최소제곱법의 관측방정식을 이용하기 위해 Visual Basic 6.0을 이용하여 좌표변환 프로그램을 개발하여 좌표를 산출하고 GPS 성과 및 현행삼각점 성과와 비교 분석하여 최적기준점을 선정하였다.

• 한국학교ㆍ지역보건교육학회지
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• 제17권2호
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• pp.1-16
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• 2016

Objectives: The objective of this study is to evaluate the correlates of depression and perception gap of adolescents' Health-Related Quality of Life (HRQOL) between Parents and Children. Methods: Research targets are all students and their parents who are enrolled in from 1st~3rd grade of middle school to $1^{st}$ grade of high school which was selected intentionally. The survey was conducted from May $16^{th}$ to June $9^{th}$ in 2014 by research staffs equipped with structured questionnaire. $PedsQL^{TM}4.0$ Generic Core Scales and Korean form of the Kovacs' Children's Depression Inventory were the main tool for measuring the HRQOL and depression. Data of 985 respondents, both of parents and children, was used for analysis of this study. Results: In terms of gender difference of depression, 5.8% of adolescents, 3.2% of male and 8.5% of female (p<0.001), are shown depression symptom. Depression rate of the low level students of academic achievement was 11.0% which is the lowest rate to compare to 4.3% of middle level and 2.6% of high level students (p<0.001). The case that parents overestimated their children's overall HRQOL is 3.699 times more likely to have depression symptom than the case which is in concurrence between parents and children. Especially the overestimation in social, emotional, and academic area of HRQOL promote depression 3.918 times, 3.371 times, and 2.258 times more than the agreement occurred of each areas. In contrast underestimation in physical area of HRQOL reduce depression 0.266 times less than the agreement occurred of the area. Conclusion: The perception gap of adolescents' HRQOL between parents and children is the most influential factor to adolescents' depression among all variables which was evaluated in this study.

• 한국수자원학회논문집
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• 제32권2호
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• pp.163-176
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• 1999

GIS기법을 이용하여 추출되는 수문지형정보는 기존 주제도의 선정, 사용된 알고리즘 등에 따라 달라질 수 있다. 따라서 본 연구에서는 토양도 및 토지이용도의 선정에 따른 유출수문곡선번호의 변화를 분석하여 미계측 유역의 강우유출모의에 효과적인 GIS의 활용방안을 제시하고자 한다. 이를 위해 미계측 유역에서의 유효강우량 산정에 필요한 GIS 공간자료(개략토양도, 정밀토양도, 토지이용계획도,위성영상)를 구축하였다. 미국 토양보존국(SCS)의 유출곡선번호(runoff curve number; CN)방법의 적용을 위한 수문학적 속성의 입력과정에서 발생할 수 있는 문제점을 분석하였다. 또한 SCS CN값 산정을 위한 GIS 공간자료의 선정에 따른 유출응답특성을 검토하였다. 실측 수문곡선과의 검증을 통해 미계측 유역에서의 강우유출모의에서 GIS의 적용성을 확인할 수 있었다. SCS CN값을 산정하기 위한 GIS 공간자료로서는 정밀토양도와 위성영상자료를 이용하는 것이 적합한 것으로 나타났다.

• 생태와환경
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• 제42권1호
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• pp.67-74
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• 2009

To study the influence of the rotifer Brachionus calyciflorus on harmful algal bloom suppression, we focused on assessing the rotifer's abilities using several prey species : Microcystis aeruginosa, Synechocystis sp., Chlorella vulgaris and Coelastrum sp. of the warm-weather species and the cold-weather centric diatom Stephanodiscus hantzchii. Grazing effects and growth rates of rotifers B. calyciflorus were 94.5% and $1.29d^{-1}$, respectively, for Synechocystis sp., 87.4% and $0.60d^{-1}$, respectively, for M. aeruginosa, 95.2% and $0.65d^{-1}$, respectively, for C. $vulgaris^{TM}$, 78.6% and $0.45d^{-1}$, respectively, for C. vulgaris UTEX., 86.5% and $0.99d^{-1}$, respectively, for Coelastrum sp., and 82.6% and $0.40d^{-1}$, respectively, for S. hantzchii. Of these, although the growth of Synechocystis and Coelastrum was effectively suppressed by rotifer grazing, efficient suppression effects on Stephanodiscus blooms were unexpected. The present study revealed that reproduction of B. calyciflorus was greatly influenced by its food types in the initial stages and the efficiencies of bio-agents as sole food sources vary depending on the target algae and the agent.

• Biomedical Engineering Letters
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• 제8권4호
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• pp.365-371
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• 2018

Uninterrupted monitoring of multiple subjects is required for mass causality events, in hospital environment or for sports by medical technicians or physicians. Movement of subjects under monitoring requires such system to be wireless, sometimes demands multiple transmitters and a receiver as a base station and monitored parameter must not be corrupted by any noise before further diagnosis. A Bluetooth Piconet network is visualized, where each subject carries a Bluetooth transmitter module that acquires vital sign continuously and relays to Bluetooth enabled device where, further signal processing is done. In this paper, a wireless network is realized to capture ECG of two subjects performing different activities like cycling, jogging, staircase climbing at 100 Hz frequency using prototyped Bluetooth module. The paper demonstrates removal of baseline drift using Fast Fourier Transform and Inverse Fast Fourier Transform and removal of high frequency noise using moving average and S-Golay algorithm. Experimental results highlight the efficacy of the proposed work to monitor any vital sign parameters of multiple subjects simultaneously. The importance of removing baseline drift before high frequency noise removal is shown using experimental results. It is possible to use Bluetooth Piconet frame work to capture ECG simultaneously for more than two subjects. For the applications where there will be larger body movement, baseline drift removal is a major concern and hence along with wireless transmission issues, baseline drift removal before high frequency noise removal is necessary for further feature extraction.

• 멤브레인
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• 제28권6호
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• pp.414-423
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• 2018

본 연구에서는 염색 염료 산업의 폐수 처리 및 재활용 공정에 분리막을 적용하고자 실란 커플링제를 이용하여 상용화된 나노복합막을 표면 개질하였다. 실란 커플링제는 말단 관능기가 다른 octyltrimethoxysilane (OcTMS)와 (3-aminopropyl) trimethoxysilane (APTMS)을 사용하였으며, 표면 개질을 통해 염료 분리 및 내오염성을 향상시키고자 하였다. XPS, FE-SEM, EDX 분석을 통하여 막 표면의 화학 구조 변화 및 실란 적층을 확인하였고, AFM 분석을 통해 개질막의 표면 모폴로지를 확인하였다. Zeta potential을 통해 실란 개질 막이 상용막 대비 표면 전하가 중성으로 변하는 것을 확인하였다. 그 결과, OcTMS와 APTMS로 개질한 막의 내오염성은 NE70에 비해 약 2배 이상 향상되었다. 또한, 실란으로 표면 개질한 나노복합막은 음이온 염료(Orange II) 용액에서 약 90% 이상, 양이온 염료(Safranin-O) 용액에서 약 98% 이상의 염료 제거율을 나타내어 양이온 염료 용액 처리에 적합한 것을 확인하였다.

• 한국어병학회지
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• 제35권2호
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• pp.157-165
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• 2022

Single-cycle viruses generated by reverse genetic technology are replication-incompetent viruses due to the elimination of gene(s) essential for viral replication, which provides a way to overcome the safety problem in attenuated viruses. Infectious hematopoietic necrosis virus (IHNV) is a major pathogen causing severe damage in cultured salmonid species. In the present study, we generated a single-cycle IHNV lacking the transmembrane and cytoplasmic domain in the G gene (rIHNV-GΔTM) and evaluated the prophylactic potential of rIHNV-GΔTM in rainbow trout (Oncorhynchus mykiss). To produce rIHNV-GΔTM, IHNV G protein-expressing Epithelioma papulosum cyprini (EPC) cells were established. However, as the efficiency of rIHNV-GΔTM production in EPC cell clones was not high, fish were immunized with a low-tittered single-cycle virus (1.5 × 10² PFU/fish). Despite the low dose, the single-cycle IHNV induced significant protection in rainbow trout against IHNV infection, suggesting high immunogenicity of rIHNV-GΔTM. No significant difference in serum ELISA titers against IHNV between the rIHNV-GΔTM immunized group and the control group suggests that the immunized dose of rIHNV-GΔTM might be too low to induce significant humoral adaptive immune responses in rainbow trout. The involvement of adaptive cellular immunity or innate immunity in the present significantly higher protection by the immunization with rIHNV-GΔTM should be further investigated to know the protection mechanism.

• 대한통합의학회지
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• 제10권1호
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• pp.39-48
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• 2022

Purpose : This study aimed to determine the experiences of the disaster response team 9 participants who participated in the disaster after the fire incident occurred in Jecheon Sports Center and their meaning and essence. Methods : Nine disaster response teams were the subjects of the study, and Individual in-depth interviews were conducted. Data were collected online between January 6 and April 30, 2021, from the software Zoom^TM. This study was conducted as a qualitative study by applying Giorgi's phenomenological experiential research method, which has an advantage in revealing the essential structure and meaning of experience. Results : Three major themes were derived from the study results: the stimulus through unfamiliar experiences, the grievances due to work environment and regional characteristics, and dullness resulting from hiding and enduring. The following 17 sub-themes were identified: learning through unfamiliar experiences, frustration due to unexpected circumstances, shock from unfamiliar experiences, doing my best in the present, confidence due to increased experience, disunified system, intervention of various interests, the atmosphere that puts responsibility on others, inactive help, unforgettable regional characteristics, working without time to settle, tolerating it in my own way, memories left in the unconsciousness, sudden suffering from memories that come to mind, movement in anxiety and tension, dullness, work with colleagues in the same situation. Conclusion : This study is meaningful in that it attempted to provide basic data in preparing a long-term strategy for effective policy direction and institutional protection based on the systematic mental health management of the disaster response team. Additionally, this study's results can be used as primary data for future research among Disaster Response Team.