• Analytical Science and Technology
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• v.24 no.1
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• pp.51-59
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• 2011

Genetic Identification has become an important forensic investigation method which discerns identity through analysis of physical samples discovered in various crime scenes. Recently more samples are being requested to undergo A-STR analysis of low copy number (LCN) DNA, which is known as touch evidence-type sample and left on various objects such as a pen briefly used by the criminal, the gear of the car used for driving, the handle, and various buttons inside a car. This research attempted to extract the LCN DNA of the touch evidencetype left on crushed fingerprints on firearms, etc. and examine the genotyping success rate. Four types of firearms (M16, K1A, COLT 45 Pistol, M29 Revolver) were fired individually and physical samples were gathered from four parts of each firearm. Subsequently, in order to extract the LCN DNA, Microkit and $Prepfiler^{TM}$ were used to compare and analyze the quantity of DNA extracted and the genotyping success rate. Analysis results showed that the quantity of DNA extracted by $Prepfiler^{TM}$ was on average 1.7 times higher than that of Microkit, and in genotype analysis success rate $Prepfiler^{TM}$ also demonstrated 24.9% on average in contrast to 0% for Microkit. In regards to the grip part of the K1A, $Prepfiler^{TM}$'s success rate was as high as 50.6%.

• Journal of Pharmaceutical Investigation
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• v.34 no.1
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• pp.49-55
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• 2004

To develop a aceclofenac soft capsule, four preparations with various solubilizers were prepared and their dissolution test was carried out. Among four preparations tested, a preparation with ethanolamine was selected as a formula of aceclofenac soft capsule (Clanza $S^{TM}$), since it showed the fastεst dissolution rate. Bioequivalence of aceclofenac tablet, $Airtal^{TM}$ (Dae-Woong Pharmaceutical Co., Ltd.) and aceclofenac soft capsule, Clanza $S^{TM}$ (Korea United Pharmaceutical Co., Ltd.) was evaluated according to the guideline of KA Fourteen normal male volunteers (age 20 - 25 years old) were divided into two groups and a randomized $2{\times}2$ cross-over study was employed. After oral administration of one tablet or capsule containing 100 mg of aceclofenac, blood was taken at predetermined time intervals and the concentration of aceclofenac in plasma was determined with an HPLC method under UV detector The pharmacokinetic parameters ($C_{max}$ and $AUC_t$) were calculated and ANOVA was utilized for the statistical analysis of parameters using logarithmetically transformed $AUC_t$, $C_{max}$ and $T_{max}$. The results showed that the differences in $AUC_t$, $C_{max}$ and $T_{max}$ between Aral tablet and Clanza soft capsule were 2.89%, 0.18% and 43.0%, respectively. There were no sequence effects between two formulations in these parameters. The 90% confidence intervals using logarithmically transformed data were within the acceptance range of log(0.8) to log(15) (e.g. log(0.81) -log(1.23) ad log(0.89) -log(1.4)) fo $AUC_t$ and $C_{max}$, respectively. Thus, the criteria of the KFDA guidelines for the equivalence was satisfied, indicating that Clanza $S^{TM}$ soft capsule is bioequivalent to$Airtal^{TM}$ tablet.

• Proceedings of the KSRS Conference
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• 2003.11a
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• pp.13-14
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• 2003

Burgess?s concentric zone model is a famous theory in the geography of city. However, the zone border line drawing method was uncertain. We have developed the method of drawing the border using land use images.

• Proceedings of the Korea Water Resources Association Conference
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• 2006.05a
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• pp.1940-1944
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• 2006

현지관측을 통한 지속적이고 광범위한 지역에 대해 정확하고 정밀하게 조사하여 종합적인 분석과 예측, 결정과정에 있어서, 복잡한 해양의 특성, 여러가지 조사 작업상의 난점, 경제적, 시간적으로 많은 어려움이 따르게 된다. 하지만, 위성원격탐사와 GIS를 이용한 해양환경파악기법은 현지관측에서 얻을 수 있는 제한적인 자료이외의 다량의 자료를 정성 및 정량적으로 데이터베이스화하여 분석함과 동시에 가시화함으로써 해양개발로 인해 불가피하게 초래될 수밖에 없는 환경을 보다 정확하게, 객관적으로 분석하여 장기적으로 예측할 수 있는 고도화된 환경조사 및 평가 기술이라고 할 수 있다. 본 연구에서는 고해상도 위성자료인 Landsat TM 영상과 NOAA AVHRR 자료를 이용하여 수온 및 클로로필을 추출하였으며, GIS를 이용하여 현지관측자료 및 수치해도를 기초로 공간분포도를 작성함으로서 그 외의 수질환경요소를 산출하였다. 위성영상분석은 현장조사와 같은 시점의 Landsat TM 위성영상을 획득하여, 위성 영상은 지구의 곡률과 자전, 위성체의 자세와 고도 및 속도, 그리고 센서의 기하 특성으로 인하여 실제의 지형에 대하여 기하학적 왜곡을 가지고 있으므로 지형도에서 지상기준점(Ground Control Point, GCP)를 추출하여 ERDAS Imagine으로 UTM좌표체계에 따른 기하보정(Geometric Correction)을 실시하였으며, 동일한 시기의 NOAA AVHRR영상을 데이터로 처리하여 수온자료를 추출하였다. 표층수온과 현장관측에 의한 클로로필을 수치 지도화하기 위하여 열적외선영역인 TM band 6의 분광특성값(Digital Number)과 동일한 위치의 수온자료를 기초로 회귀분석을 실시함으로써 수온추출 알고리즘을 도출하여, 분석데이터의 신뢰도를 검증하였으며, 수온, 클로로필, 투명도 등을 위성원격탐사 자료와 GIS를 이용하여 공간분석을 실시하고, 공간분포도를 작성함으로써 대상해역의 해양환경을 파악하였다. 본 연구결과, 분석된 위성자료가 현장조사에 의한 검증이 이루어지지 않을 경우, 영상자료분석을 통한 표층수온 추출은 대기 중의 수증기와 에어로졸에 의한 계산치의 오차가 반영되기 때문에 실측치 보다 낮게 평가 될 수 있으므로, 반드시 이에 대한 검증이 필요함을 알 수 있었다. 현지관측에 비해 막대한 비용과 시간을 절약할 수 있는 위성영상해석방법을 이용한 방법은 해양수질파악이 가능할 것으로 판단되며, GIS를 이용하여 다양하고 복잡한 자료를 데이터베이스화함으로써 가시화하고, 이를 기초로 공간분석을 실시함으로써 환경요소별 공간분포에 대한 파악을 통해 수치모형실험을 이용한 각종 환경영향의 평가 및 예측을 위한 기초자료로 이용이 가능할 것으로 사료된다.

• Journal of Life Science
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• v.14 no.2
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• pp.309-314
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• 2004

Cyclic AMP receptor protein (CRP) is involved in the transcriptional regulation of more than 100 genes in E. coli. CRP dimer is converted into active form via the sequential conformation change of cAMP binding pocket, hinge region and HTH DNA binding motif by binding of cAMP. The temperature gradient gel electrophoresis (TGGE) was applied to CRP protein to know whether it was an efficient technique to study the conformational transitions and the thermal stability. TGGE showed the unfolding process of wild-type and S83G CRP proteins with the temperature gradient set from 29 to 71$^{\circ}C$ on nondenaturing polyacrylamide gel. Melting temperature (Tm) was 57$\pm$1 and 55$\pm$1$^{\circ}C$ for wild-type and S83G CRP, respectively in acidic buffer[89.8 mM Glycine and 24 mM Boric acid (pH 5.8)].

• Journal of the Korea Society for Simulation
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• v.22 no.2
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• pp.63-71
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• 2013

Developing future weapons systems has become increasingly complicated and costly. There, modeling and simulation techniques have been highly interested in developing the defense systems. Modeling and simulation techniques provide a means to simulate military training, strategies, military doctrines, and weapons acquisition. In this paper, we proposed a small scale engagement scenario generation method for engagement M&S model. Generated scenario is one of critical factors in the field of commander training, operational analysis, and tactical evaluation. The objective of this paper is to develop a scenario generation method for small scale engagement using the FSA(Finite State Automata) and DFS(Depth First Search) algorithm. The proposed method is verified using a one-on-one combat engagement scenario between assault ship and reconnaissance ship. Also, we are visualized using Delta3D$^{TM}$.

• Restorative Dentistry and Endodontics
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• v.25 no.3
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• pp.390-398
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• 2000

The purpose of this study was to evaluate the apical fitness of non-standardized gutta-percha cones in root canals prepared with rotary Ni-Ti root canal instruments of various tapers and apical tip sizes. Simulated sixty curved root canals of plastic blocks were prepared with crown-down technique using rotary root canal instruments of Maillefer ProFile$^{(R)}$ .04 and .06 taper (Maillefer Instrument SA, Switzerland). Specimens were divided into six groups and prepared as follows: Group 1, prepared up to size 25 of .04 taper ; Group 2, prepared up to size 30 of .04 taper ; Group 3, prepared up to size 35 of .04 taper ; Group 4, prepared up to size 25 of .06 taper ; Group 5, prepared up to size 30 of .06 taper ; Group 6 ; prepared up to size 35 of .06 taper. After cutting off the coronal portion of plastic, blocks perpendicular to the long axis of the canal with the use of a diamond saw, apical 5mm of canal space was analyzed. Prepared apical canal spaces were duplicated using rubber base impression material to evaluate two dimensional total area of apical canal space. Various sized gutta-percha cones were applied in the 5mm-apical canal space, which were size 25, size 30 and size 35 standardized gutta-percha cone, Diadent Dia-Pro ISO-.04$^{TM}$ and .06$^{TM}$(Diadent, Korea), and medium-fine (MF), fine (F), fine-medium (FM) and medium (M) sized non-standardized gutta-percha cones (Diadent, Korea). Coronal excess gutta-percha were cut off with a sharp blade. Photographs of impressed apical canal spaces and gutta-percha cones were taken with a CCD camera under a stereomicroscope and stored in a computer. Areas of the total canal space and gutta-percha cones were calculated using a digitalized image analysing program, CompuScope (Sungjin Multimedia Co., Korea). Ratio of apical fitness was obtained by calculating the area of gutta-percha cone to the total area of the canal space. The data were analysed statistically using One-way Analysis of Variance and Duncan's Multiple Range Test. The results were as follows: 1. In canals prepared up to size 25 ProFile$^{(R)}$ of .04 taper, non-standardized MF and F cones occupied significantly more canal space than Dia-Pro ISO-.04$^{TM}$ or size 25 standardized ones (p<0.05). 2. In canals prepared up to size 30 ProFile$^{(R)}$ of .04 taper, non-standardized F cones occupied significantly more canal space than Dia-Pro ISO-.04$^{TM}$ or size 30 standardized ones (p<0.05), and non-standardized MF cones occupied more canal space than size 30 standardized ones (p<0.05). 3. In canals prepared up to size 35 ProFile$^{(R)}$ of .04 taper, there was no significant difference in canal space occupation among non-standardized MF and F, size 35 standardized, and Dia-Pro ISO-.04$^{TM}$ cones (p>0.05). 4. In canals prepared up to size 25 ProFile$^{(R)}$ of .06 taper, non-standardized MF and F cones occupied significantly more canal space than Dia-Pro ISO-.06$^{TM}$, or size 25 standardized ones (p<0.05), and Dia-Pro ISO-.06$^{TM}$, cones occupied significantly more space than size 25 standardized ones (p<0.05). 5. In canals prepared up to size 30 ProFile$^{(R)}$ of .06 taper, non-standardized FM cones occupied significantly more canal space than Dia-Pro ISO-.06$^{TM}$ or size 30 standardized ones (p<0.05), and non-standardized F cones occupied significantly more canal space than size 30 standardized ones (p<0.05). 6. In canals prepared up to size 35 ProFile$^{(R)}$ of .06 taper, non-standardized M and FM, Dia-Pro ISO-.06$^{TM}$ occupied significantly more canal space than size 35 standardized ones (p<0.05). In summary, in both canals prepared with .04 or .06 taper ProFile$^{(R)}$, non-standardized cones showed better fitness than Dia-Pro ISO$^{TM}$ or standardized ones, which was more characteristic in smaller canals.

• Korean Journal of Soil Science and Fertilizer
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• v.42 no.2
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• pp.79-87
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• 2009

Pot experiments using sand culture were conducted in 2004 under greenhouse conditions to evaluate the effect of nitrogen deficiency on red pepper biomass. Nitrogen stress was imposed by implementing 6 levels (40% to 140%) of N in Hoagland's nutrient solution for red pepper. Canopy reflectance measurements were made with hand held spectral sensors including $GreenSeeker^{TM}$, $Crop\;Circle^{TM}$, and $Field\;Scout^{TM}$ Chlorophyll meter, and a spectroradiometer as well as Minolta SPAD-502 chlorophyll meter. Canopy reflectance and dry weight of red pepper were measured at five growth stages, the 30th, 40th, 50th, 80th and 120th day after planting(DAT). Dry weight of red pepper affected by nitrogen stress showed large differences between maximum and minimum values at the 120th DAT ranged from 48.2 to $196.6g\;plant^{-1}$, respectively. Several reflectance indices obtained from $GreenSeeker^{TM}$, $Crop\;Circle^{TM}$ and Spectroradiometer including chlorophyll readings were compared for evaluation of red pepper biomass. The reflectance indices such as rNDVI, aNDVI and gNDVI by the $Crop\;Circle^{TM}$ sensor showed the highest correlation coefficient with dry weight of red pepper at the 40th, 50th, and 80th DAT, respectively. Also these reflectance indices at the same growth station was closely correlated with dry weight, yield, and nitrogen uptake of red pepper at the 120th DAT, especially showing the best correlation coefficient at the 80th DAT. From these result, the aNDVI at the 80th DAT can significantly explain for dry weight of red pepper at the 120th DAT as well as for application level of nitrogen fertilizer. Consequently ground remote sensing as a non-destructive real-time assessment of plant nitrogen status was thought to be a useful tool for in season nitrogen management for red pepper providing both spatial and temporal information.

• Korean Journal of Remote Sensing
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• v.12 no.2
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• pp.139-154
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• 1996

In order to evaluate the sensor`s look direction bias in the Landsat TM image and to estimate trends of primary geological lineaments, we have attempted to systematically compare lineaments in TM image, relief shadowed DEM's, and actual lineaments of geologic and topographic map through the Hough transform technique. Hough transform is known to be very effective to estimate the trend of geological lineaments, and help us to obtain the true trends of lineaments. It is often necessary to compensate the preferential enhancements of terrain lineaments in a TM image occurred by to look direction bias, and that can be achieved by utilizing an auxiliary data. In this study, we have successfully adopted the relief shadowed DEM in which the illuminating azimuth angle is perpendicular to look direction of a TM image for assessing true trends of geological lineaments. The results also show that the sum of four relief shadowed DEM's directional components can possibly be used as an alternative. In Euiseong-gun area where Sindong Group and Mayans Group are mainly distributed, geological lineaments trending $N5^{\circ}$~$10^{\circ}$W are dominant, while those of $N55^{\circ}$~$65^{\circ}$ W are major trends in Cheongsong-gun area where Hayang Group, Yucheon Group and Bulguksa Granite are distributed. Using relief shadowed DEM as an auxiliary data, we found the $N55^{\circ}$~$65^{\circ}$ W lineaments which are not cleanly observed in TM image over Euiseong-gun area. Compared with the trend of Gumchon and Gaum strike-slip faults, these lineaments are considered to be an extension of the faults. Therefore these strike-slip faults possibly extend up to Sindong Group in the northwest parts in the study area.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.40 no.2
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• pp.195-201
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• 2014

Body fluid has been studied for diverse fields like Ringer's solutions, artificial joint fluids, cell growth culture media because it plays a crucial role in controlling body temperature and acts as a solvent for diverse metabolite processes in the body and delivery media of mineral, energy source, hormone, signal and drug from and to cell via blood or lymphatic vessel by osmotic pressure or active uptake. Stratum corneum containing extracellular lipids and NMF (natural moisturizing factor) absorbs atmospheric water residing outside of cells and utilize it to hydrate inside of their own. This process is related to skin barrier function. In this study, we conducted the cell viability test with Cell Bio Fluid $Sync^{TM}$, which mimicks body fluids including amino acids, peptides, and monosaccharides to strengthen skin barrier, and the clinical skin improvement test with cosmetics containing Cell Bio Fluid $Sync^{TM}$. In the cell viability test, HaCaT cell was treated with PBS for 3 hours, followed by the treatment of a cell culture medium (DMEM) and isotonic solution (PBS) and Cell Bio Fluid $Sync^{TM}$ for 3 hours each. Then, MTT assay and image analysis were conducted. In the clinical skin improvement test, twenty-one healthy women participated. Participants applied cosmetics containing Cell Bio Fluid $Sync^{TM}$ on their face for a week and evaluated the skin hydration, skin roughness, brightness and evenness. All measurements were conducted after they washed off their face and took a rest under the constant temperature ($22{\pm}2^{\circ}C$) and constant humidity conditions ($50{\pm}5%$) for 20 minutes. All the data were analyzed by SPSS (version 21) software program. Results showed that Cell Bio Fluid $Sync^{TM}$ improved both the cell viability and in vivo skin conditions such as skin hydration, roughness, brightness and evenness.