• 분석과학
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• 제28권2호
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• pp.79-85
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• 2015

TLC (SiO₂) 상에서 3가지 유사 암페타민 류 아민 화합물과 3가지 유도체화 시약 사이의 반응으로부터 얻어진 아민유도체의 이미지 분석으로부터 암페타민 류 화합물에 대한 신속하고 경제적인 반정량적인 분석방법을 제시하기 위하여 선행 실험을 하였다. TLC상 직접적인(in situ, co-spot) 유도체화 반응의 적정화를 시도하였고 그 TLC 결과물들을 디지털카메라로 촬영하여 이미지를 얻은 후 2가지의 이미지 분석 프로그램(CP Atlas 2.0 및 ImageJ)에 적용하여 재현성(RSD; %)과 상관성(R²)을 각각 확인 및 비교하였다. 그 결과, 반응 조건에 대한 조정이 필요하였고(반응 온도 등) 각 시료별 2가지의 농도(0.5 mg/mL 및 0.01 mg/mL)에서 반응의 재현성은 0.69~5.50% 범위이었으며 반응(2 µL per spot)의 생성물과 아민의 농도에 대한 상관성은 0.1~0.005 mg/mL의 농도 구간에서 가장 높게 나타났다(R²> 0.9906). 또한, 두 가지 프로그램 모두 재현성과 직선성에 대한 유사한 결과를 나타내어 직접적인 유도체화 반응과 TLC 이미지에 대한 이들 프로그램을 사용한 분석이 아민 화합물에 대한 반정량적인 분석으로 사용될 수 있는 가능성을 보였다.

• 동력기계공학회지
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• 제20권6호
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• pp.51-57
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• 2016

Experiment is carried out to investigate the mixed convective flow in three-dimensional horizontal rectangular channels filled with high viscous fluid. The particle image velocimetry(PIV) with thermo-sensitive liquid crystal tracers is used for visualizing and analysis. Quantitative data of temperature and velocity are obtained by applying the color-image processing to a visualized image, and neural network is applied to the color-to-temperature calibration. In this study, the fluid used is silicon oil(Pr=909), the aspect ratio(channel width to heigh) is 4 and Reynolds number is $2{\times}10^{-2}$. From the present study, we can visualize the quantitative temperature and velocity of mixed convective flow in three-dimensional horizontal rectangular channels simultaneously.

• 동력기계공학회지
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• 제9권2호
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• pp.33-39
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• 2005

Experiments are performed to study the mixed convection flow and heat transfer in an inclined parallel plates with the upper part cooled and the lower part heated uniformly. The Reynolds number ranges from $4.0{\times}10^{-3}\;to\;6.2{\times}10^{-2}$, the angle of inclination, ${\theta}$, from 0 to 45 degree from the horizontal line, and Pr of the high viscosity fluid is 909. In this paper, the PIV(Particle image velocimetry) with TLC(Thermo-sensitive liquid crystal) tracers is used for visualizing and analysis. This method allows simultaneous measurement of velocity and temperature field at a given instant of time. Quantitative data of the temperature and velocity are obtained by applying the color-image processing to a visualized image, and neural network is applied to the color-to-temperature calibration. This paper describes the methods, and presents the quantitative visualization of mixed convection. From this study, it is found that the flow pattern can be classified into three patterns which are affected by Reynolds number and the angle of inclination.

• 설비공학논문집
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• 제18권9호
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• pp.698-706
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• 2006

Experiment and numerical calculation have been peformed to investigate mixed convection heat transfer between inclined parallel plates. Particle image velocimetry (PIV) with thermo-sensitive liquid crystal (TLC) tracers is used for visualizing and analysis. This method allows simultaneous measurement of velocity and temperature fields at a given instant of time. Quantitative data of the temperature and velocity are obtained by applying the color-image processing to a visualized image, and neural network is applied to the color-to-temperature calibration. The governing equations are discretized using the finite volume method. The results are presented for the Reynolds number ranges from 0.004 to 0.062, the angle of inclination, ${\Theta}$, from 0 to 45 degree and Prandtl number of the high viscosity fluid is 909. The results show velocity, temperature and mean Nusselt numbers distributions. It is found that the periodic flow of mixed convection between inclined parallel plates is shown at $0^{\circ}{\leq}{\Theta}<30^{\circ}$, Re<0.062, and the flow pattern can be classified into three patterns which depend on Reynolds number and the angle of inclination. The minimum Nusselt numbers occur at Re=0.05 regardless of the angle of inclination.

• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 2003년도 춘계학술대회 논문집
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• pp.1071-1075
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• 2003

In a closed square cavity filled with a liquid, a cooled the upper horizontal wail and a heated the lower horizontal wall, the flow isn't generated under the ground-based condition when Rayleigh number is lower than 1700. In such case the flow phenomena near an air bubble under a cooled horizontal wall were investigated. The temperature and the flow fields were studied by using the Thermo-sensitive Liquid-Crystal and the image processing. The qualitative analysis for the temperature and the flow fields were carried out by applying the image processing technique to the original data. Injecting bubble at the center point of upper cooled wall, the symmetry shape of two vortexes near an air bubble was observed. The bubble size increased, the size of velocity and the magnitude of velocity increased. In spite of elapsed time, a pair of two vortexes was the unique and steady-state flow in a square cavity and wasn't induce to the other flow in the surround region.

• Journal of Advanced Marine Engineering and Technology
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• 제33권7호
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• pp.987-993
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• 2009

An experimental study is performed to investigate unsteady mixed convection in a horizontal channel with a heat source. Particle image velocimetry (PIV) with thermo-sensitive liquid crystal (TLC) tracers is used for visualization and analysis. This method allows simultaneous measurement of velocity and temperature fields at a given instant of time. Quantitative data of the temperature and velocity are obtained by applying the color-image processing to a visualized image, and neural network is applied to the color-to-temperature calibration. It is found that the periodic flow of mixed convection in a cavity appears at very low Reynolds numbers (Re<0.4), and the period decreases with increasing Reynolds numbers and increases with increasing aspect ratio.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2003년도 추계학술대회
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• pp.85-89
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• 2003

In a closed square cavity filled with a liquid, a cooled the upper horizontal wall and a heated the lower horizontal wall, the flow isn't generated under the ground-based condition when Rayleigh number is lower than 1700. In such case the flow phenomena near an air bubble under a cooled horizontal wall were investigated. The temperature and the flow fields were studied by using the Thermo-sensitive Liquid-Crystal and the image processing. The qualitative analysis for the temperature and the flow fields were carried out by applying the image processing technique to the original data. Injecting bubble at the center point of upper cooled wall, the symmetry shape of two vortexes near an air bubble was observed. The bubble size increased, the size of velocity and the magnitude of velocity increased. In spite of elapsed time, a pair of two vortexes was the unique and steady-state flow in a square cavity and wasn't induce to the other flow in the surround region.

• 공업화학
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• 제29권3호
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• pp.342-349
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• 2018

본 연구에서는 올리브 오일의 에스터 교환반응으로 생성된 복합소재(olive oil esters)의 화장품 응용을 위해 물리화학적 성질, 유화력, 보습력 및 세포독성을 평가하였다. 실험에는 반응시간이 짧은 olive oil esters S와 반응시간이 긴 olive oil esters L을 사용하였다. 먼저 TLC 이미지 분석으로 조성비를 확인한 결과, olive oil esters S의 조성은 mono-, di-, tri-glyceride가 5.2, 24.1, 46.4%이었고, fatty acid ethyl ester는 21.9%이었다. Olive oil esters L은 mono-, di-, tri-glyceride가 4.1, 24.7, 40.6%이었고, fatty acid ethyl ester는 28.8%이었다. 올리브 오일 에스터를 화장품에 사용하기 위한 기준 및 시험방법 설정을 위해 요오드가, 산가, 검화가, 불검화물, 굴절률, 비중 및 순도시험을 수행하였고 표준화시켰다. 또한 올리브 오일 에스터의 유화력 평가로서, 계면활성제 없이 O/W 에멀전을 제조하여 유화 입자를 확인하였다. 올리브 오일 에스터를 사람 피부에 도포 5일 후, 피부 수분 보유량은 초기보다 13.1% 개선되었다. 피부 세포에 대한 독성을 평가한 결과, 올리브 오일 에스터는 $0.2-200{\mu}g/mL$에서 세포 생존율 90% 이상을 나타내 안전성이 확인되었다. 결과적으로 올리브 오일 에스터는 화장품 산업에서 천연/무독성 원료로서 응용가능성이 있음을 시사하였다.

• 미생물학회지
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• 제35권2호
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• pp.146-152
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• 1999

충치 원인균의 일종인 Streptococcus sobrinus ATCC27351 균주를 자외선 조사와 NTG 처리에 의하여 bacitracin 내성변이주를 얻었다. 선발된 변이주들 중 glucosyltransferase 활성이 모균주보다 증가된 변이주 4균주를 선발하였다. 이들의 bacitracin 내성은 모균주 보다 5~48배, glucosyltransferase 활성은 약 2배 증가하였다. 모균주의 glucosyltransferase를 이용하여 maltose acceptor 반응을 하여 \ulcorner 와 image analysis 로 분석하였다. 그 결과 sucrose 와 acceptor를 사용하여 반응을 시킨 결과 모균주와 변이주의 glucose 전이효소 사이에는 acceptor 특이성에 차이가 있었다. 모균주의 효소와는 반응하지 않는 acceptor 인 fructose를 함유하는 1-kestose와 nystose 그리고 turanose를 acceptor 로 하여 변이주 S. sobrinus BR24C 균주의 glucosyltransferase와 반응을 시켰다. 얻어진 acceptor 산물을 정제하여 \sup 1\H, \sup 13\C-NMR 분석을 한 결과, 자연계에서는 발견되지 않는 새로운 구조의 올리고당 \6^{3}$-$\alpha$-D-glucopyranosyl \3^{2}$-O-$\alpha$-D-glucopyranosyl-D-fructose와 \6^{4}$-$\alpha$-D-glucopyranosyl \1^{3}$-$\beta$-D-fructofuranosyl sucrose을 확인하였다.