• Journal of Microbiology and Biotechnology
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• 제16권6호
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• pp.855-862
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• 2006

Hexavalent chromium ($Cr^{6+}$) is a highly harmful pollutant, which can be detoxified and precipitated through reduction to $Cr^{3+}$. Bacillus megaterium TKW3 previously isolated from chromium-contaminated marine sediments was capable of reducing $Cr^{6+}$ in concomitance with metalloids ($Se^{4+}$, $Se^{6+}$, and $As^{5+}$). Notwithstanding approximately 50% inhibition, it was the first report of simultaneous bacterial reduction of $Cr^{6+}$ and $Se^{4+}$ (to elemental Se). No significant difference was observed among electron donors (glucose, maltose, and mannitol) on $Cr^{6+}$ reduction by B. megaterium TKW3. The reduction was constitutive and determined to be non-plasmid mediated. Peptide mass fingerprints (PMF) revealed a novel aerobic membrane-associated reductase with $Cr^{6+}$-induced expression and specific reductive activity (in nmol $Cr^{6+}$/mg protein/min) of 0.220 as compared with 0.087 of the soluble protein fraction. Respiratory inhibitor $NaN_3$ did not interfere with the reductase activity. Transmission electron microscopy with energy dispersive X-ray (TEM-EDX) analysis confirmed the aggregation of reduced chromium along the intracellular membrane region. Future identification of the N-terminal amino acid sequence of this reductase will facilitate purification and understanding of its enzymatic action.

• 인터넷정보학회논문지
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• 제11권5호
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• pp.119-126
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• 2010

본 논문은 변환키 비대칭 워터마킹 시스템의 강인성을 분석하고 이에 대한 개선을 제안한다. 먼저 비대칭 워터마킹 시스템의 공개 검출을 불가능하게 하는 빼기공격의 상황을 가정하고 변환키 비대칭 워터마킹 시스템의 검출성능에 대한 척도를 제안한다. 다음으로 빼기공격에 강인하기 위하여 변환키 비대칭 워터마킹 시스템이 갖추어야 할 최적의 조건을 분석한다. 또한 변환키 비대칭 워터마킹 시스템 전체의 검출 성능을 개선하기 위하여 새로운 비공개 검출 방법을 제안한다. 제안하는 개선된 방식은 빼기공격 뿐만이 아니라 Wu의 공격에도 강인함을 보인다.