• 제목/요약/키워드: TFC

검색결과 164건 처리시간 0.032초

오이풀잎 에탄올 추출물에 대한 기능성 연구 (Biofunctional Activities of Sanguisorbae officinalis L. Leaves Ethanol Extract)

  • 박성진;나영아
    • 한국조리학회지
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    • 제21권5호
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    • pp.171-179
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    • 2015
  • 본 연구의 목적은 한국에서 재배된 70% 오이풀 에탄올 추출물을 이용하여 총페놀, 총플라보노이드, 페놀릭 화합물과 항산화성(DPPH, ABTS, FRAP, 환원력)에 대해 실험결과를 제공하는 것이다. 연구결과 총페놀과 총플라보노이드의 함량은 각각 $119.3{\pm}1.54mg$ gallic acid and $59.6{\pm}1.43mg$ rutin를 나타내었다. 또한, 추출물에 함유된 페놀릭 화합물의 주된 물질은 Catechin으로 나타났으며, 항산화성을 나타내는 DPPH, ABTS, FRAP, 환원력은 농도의존적으로 증가하는 경향을 나타내었다. 이상의 결과로 오이풀 추출물은 천연 항산화성 및 기능성 식품 첨가물로서 좋은 재료가 될 것으로 판단된다.

The Effect of Pre-Treated Black Garlic Extracts on the Antioxidative Status and Quality Characteristics of Korean Ginseng Chicken Soup (Samgyetang)

  • Barido, Farouq Heidar;Jang, Aera;Pak, Jae In;Kim, Yeong Jong;Lee, Sung Ki
    • 한국축산식품학회지
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    • 제41권6호
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    • pp.1036-1048
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    • 2021
  • This study investigated the possible improvement in the antioxidative status and quality characteristics of ready-to-eat (RTE) Samgyetang after adding various black garlic (BG) extracts. The antioxidant activity, total phenolic content (TPC), total flavonoid content (TFC), meat quality indexes, and lipid oxidation rates were measured after receiving one of five different treatments consisting of conventional Samgyetang broth as a negative control, raw garlic (RG) extract as a positive control, BG, oven-dried BG, and maltodextrin-encapsulated BG extract as treatments. Employing retort cooking, fat trimmed carcasses were added to the initially prepared broth together with a phenolic extract that was set at 5% (w/w). A significant intensification of red and yellow color was observed in breast and thigh meat treated with BG extracts, regardless of pretreatment, compared to the negative control and RG. The moisture percentage was affected by the addition of BG extracts, where the encapsulation group retained the highest water content after retorting. In terms of antioxidative status, maltodextrin-encapsulated BG extract was as effective as an oven-dried extract to scavenge free radicals and showed the highest score among samples (p<0.01). The concentration of TFC was found to be the highest and did not differ between encapsulation and oven-dried groups, followed by BG, RG, and the negative control. However, the addition of encapsulated BG extract was the most effective in delaying the formation of malondialdehyde among the samples. Therefore, pre-treatment of BG extract through encapsulation is recommended to develop a higher antioxidative status and quality characteristics of Samgyetang.

몽골과 미얀마 식물 14종의 3T3-L1 및 HepG2 세포에서 지질 축적 억제효과 (Inhibitory Effects of 14 Plants from Mongolia and Myanmar on Lipid Accumulation in 3T3-L1 and HepG2 Cells)

  • 김숙진;김건희
    • 한국식생활문화학회지
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    • 제36권1호
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    • pp.130-142
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    • 2021
  • This study examined the antioxidative and lipid accumulation inhibitory effects of 14 plants from Mongolia and Myanmar on 3T3-L1 and HepG2 cells. The total phenolic and flavonoid contents (TPC and TFC) of 14 plant extracts were measured, and the antioxidative activities were analyzed using DPPH, ABTS, FRAP, and ORAC. After measuring the pancreatic lipase levels and performing the thiobarbituric acid assay, the degree of lipid accumulation was determined by lipid (Oil Red O) staining and triglyceride assay in 3T3-L1 and HepG2 cells. M. paniculate (259.43 mgGAE/g) and C. benghalensis (130.78 mgNAE/g) had the highest TPC and TFC, respectively, among the 14 plants. R. acicularis Lindl. had the highest antioxidant activity in DPPH. The ABTS, FRAP, and ORAC results showed that the antioxidant activity of 11 species was higher than that of the positive control. The pancreatic lipase inhibitory effect of C. angustifolium Scop. was reduced to 23.65% at 0.1 mg/mL, and the level of lipid peroxidation of C. abrorescens Lam. was 0.63 nmol/mg. Five selected plants inhibited the lipid accumulation and triglyceride content, respectively, in 3T3-L1 and HepG2 cells. These results provide scientific evidence for developing functional foods using 14 plants from Mongolia and Myanmar, which have antioxidant activities and lipid accumulation reduction effects.

Inhibitory Effect of Ceylon Black Tea Extract on the Melanogenesis in 𝛼-MSH Stimulated B16F10 Melanoma Cells

  • Rathnayake, Anuruddhika Udayangani;Wickramasinghe, Indira;Byun, Hee-Guk
    • 한국해양바이오학회지
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    • 제14권1호
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    • pp.25-32
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    • 2022
  • The desire to be light skinned is universal among women. Asia has a long history of using skincare formulations as whitening agents. There is an imperative need to develop novel cosmetics from herbal sources due to several unpleasant side effects and high costs. As a result, this study aims to investigate the effect of Ceylon black tea extracts on melanogenesis. Five different Ceylon black tea extracts were prepared and examined for total phenolic content (TPC), total flavonoid content (TFC), DPPH radical scavenging activity, and tyrosinase inhibitory activity. Furthermore, B16F10 melanoma cells were treated with these extracts and tested for cytotoxicity and protein suppression levels. According to the results of this study, the highest TPCs were obtained from ethanol and acetone extractions (240.303 ± 1.389 ㎍/g and 240.202 ± 4.700 ㎍/g, respectively), whereas the highest TFC was obtained from acetone extraction (57.484 ± 0.413 ㎍/g). Ceylon black tea extracted with ethanol exhibited the highest inhibitory activity on tyrosinase with an IC50 value of 0.277 ± 0.017 mg/mL and the highest DPPH radical scavenging activity with an EC50 value of 0.009 ± 0.000 mg/mL. Furthermore, western blot results revealed that tyrosinase, TRP-1, TRP-2, and MITF protein expression levels were dose-dependently suppressed, indicating the applicability of Ceylon black tea extract as a novel melanogenesis inhibitor.

추출 온도 및 용매에 따른 아메리카동애등에(Hermetia illucens) 유충 추출물의 항산화 효과 (Antioxidant Effects of Hermetia illucens Larvae Extracts Using Different Extraction Temperatures and Solvents)

  • 박지영;구본우;김용순;박관호
    • 한국환경과학회지
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    • 제32권4호
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    • pp.221-232
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    • 2023
  • This study investigated the antioxidant effect of Hermetia illucens larvae using different extraction temperatures and solvents. We found significant differences in total phenolic content (TPC), total flavonoid content (TFC), an in three antioxidant indexes 2,2-diphenyl-1-picrylhydrazyl (DPPH), 1,1'-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid (ABTS), and ferric-reducing antioxidant power (FRAP) contents, among the samples depending on the extraction temperature and solvent used. The sample extracted with water at 45℃ (HIW-45℃) showed the highest TPC, DPPH, ABTS, and FRAP contents, while the sample extracted with water at 90℃ (HIW-90℃) showed the highest TFC. These differences can be due to the different chemical structures of the extracted components. Based on these results, HIW-45℃ was the optimal extraction method for Hermetia illucens. We intend to further investigate the availability of functional materials for Hermetia illucens using this method.

토사 함량에 따른 자갈 성토재료의 침하특성 분석 (The Influence of Soil Content on the Settlement Behavior of Gravel Embankement)

  • 이수형;김지호;김범준;윤찬영
    • 한국지반환경공학회 논문집
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    • 제24권11호
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    • pp.41-49
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    • 2023
  • 본 연구에서는 토사가 혼합된 암성토 제방의 침하 특성을 분석하기 위하여 실트질 재료 혼합에 따른 토사재료의 물리적 특성을 확인하고, 토사재료 혼합 비율에 따른 자갈재료의 압축특성을 분석하였다. 이를 위하여 사질토에 실트질 재료를 혼합하여 토사재료의 압축특성을 분석하였으며, 도상자갈과 유사한 입도분포를 갖는 암성토 재료에 다양한 비율의 토사를 혼합하여 지반을 조성하고 중형챔버를 이용한 일차원 압축실험을 수행하였다. 실험결과, 혼합토사 재료의 경우 Transition Fine Content(TFC)는 하중 조건에 따라서 21~26% 범위로 나타났으며, 토사가 혼합된 암성토 재료의 경우, 자갈 시료 내 토사의 공극 채움비율이 증가함에 따라 총압축량과 크리프 압축이 모두 감소하다가 50% 혼합비 이후에는 다시 침하량이 증가하는 것으로 나타났다.

Comparison of Bioactive Compounds and Antioxidant Activity according to Culture Systems in Artemisia fukudo

  • Eun Bi Jang;Jong-Du Lee;Hyejin Hyeon;Yong-Hwan Jung;Weon-Jong Yoon
    • 한국자원식물학회:학술대회논문집
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    • 한국자원식물학회 2022년도 추계학술대회
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    • pp.99-99
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    • 2022
  • Artemisia fukudo is a biennial plant and has been reported to have anticancer, anti-melanogenesis, and anti-inflammatory effects. However, it is difficult to produce biomass from A. fukudo, so it is not used as a material for cosmetics or pharmaceuticals. In vitro culture can stably produce biomass throughout the year. In this study, the culture system for producing the highest biomass and bioactive substances was compared. Ex vitro plants were collected in Pyoseon-eup, Jeju island in May 2021, and in vitro culture was harvested after culturing for 8 weeks (plantlet) and 4 weeks (adventitious roots), respectively. After harvest, total polyphenol content (TPC), total flavonoid content (TFC), and DPPH scavenging activity were analyzed. In biomass production, adventitious roots (FW: 5.1 g·100 ml-1, DW: 0.6 g·100 ml-1) were about 4 times higher than that of plantlets (FW: 1.8 g·200 ml-1, DW: 0.3 g·200 ml-1). Both TPC and TFC were highest in ex vitro plants (9.2 ㎍·mL-1, 31.6 ㎍·mL-1), and were 3.0 times and 1.8 times higher than those of plantlets (3.0 ㎍·mL-1, 17.8 ㎍·mL-1), respectively. The IC50 value of DPPH scavenging activity was also the best in ex vitro plants (69.8 ㎍·mL-1), followed by root root (184.4 ㎍·mL-1) and plants (325.3 ㎍·mL-1) in that order. Through additional elicitor treatment, scale-up, and advanced compounds analysis such as HPLC, it can be used as an industrial material.

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블랜칭 처리가 비트의 품질특성과 항산화 활성에 미치는 영향 (Effect of Blanching Treatment on Quality Characteristics and Antioxidant Activity of Beetroots)

  • 최해연;김수빈;고은성;추지혜;전희경;최진희
    • 한국식생활문화학회지
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    • 제38권6호
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    • pp.434-441
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    • 2023
  • This study investigated quality characteristics and antioxidant activity of beetroots after blanching. Beetroots were blanched in distilled water, 2% NaCl water, and 2% citric acid water at 100℃ for 3 minutes (the blanched group). The moisture content was highest in the control (CON) at 91.30% (p<0.05), and cooking loss was lowest in the water-blanched beetroot (BW) at 5.39% (p<0.01). Chromaticity decreased after blanching compared to CON (p<0.001). Total polyphenol contents (TPC) and total flavonoid contents (TFC) decreased after blanching, and as a result of comparing the True retention (TR) of the blanching treatment group, BW had the highest with TPC TR 91.22% and TFC TR 70.51%. DPPH and ABTS+radical scavenging activities were highest in the CON, and in the blanching group BW was highest scavenging activity. The total number of microorganisms in the CON group was 2.97 log CFU/g, whereas no microorganisms were detected in the blanched groups. Therefore, this study, blanching in water without additives is the most appropriate method for preserving physiologically active substances and nutrients in beetroots and inhibiting microbial growth.

디지털 지형 고도 데이터를 이용한 자동 지형 추종 시뮬레이터 개발 (Development of Automatic Terrain Following Simulator Using Digital Terrain Elevation Data)

  • 이지수;유문규;이현주;송기훈;전동익;이상철
    • 항공우주시스템공학회지
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    • 제18권1호
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    • pp.88-98
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    • 2024
  • 본 논문에서는 디지털 지형 고도 데이터를 이용한 자동 지형 추종 시뮬레이터를 제안하였다. ATF Simulator는 Flight Simulator, Radar Simulator, TFC Simulator로 구성하였다. 지형 추종에 필요한 지형 정보로 디지털 지형 고도 데이터와 디지털 지형 고도 데이터를 이용하여 생성한 레이다 스캔 데이터를 활용하였다. ATF Simulator는 디지털 지형 고도 데이터를 이용하는 Passive mode, 레이다 스캔 데이터를 이용하는 Active mode, 두 가지 모두 이용하는 Hybrid mode로 세 가지 운용모드를 제공한다. LabVIEW 개발 환경과 MATLAB App Designer 개발 환경을 통해 지형 추종 시스템 개발에 소요되는 비용 및 시간을 줄일 수 있는 ATF Simulator를 개발하였으며 기능요구사항을 충족하는지 확인하여 검증하였다.

Effect of pretreatment conditions on quality characteristics and antioxidant activity in pak choi (Brassica rapa L. ssp. chinensis)

  • Jin-Hee Choi;Hyun-do Ahn;Hae-Yeon Choi
    • 한국식품저장유통학회지
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    • 제30권6호
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    • pp.969-982
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    • 2023
  • This study evaluated the impact of blanching pak choi stems and leaves in various solutions (distilled water, 2% NaCl, and 2% citric acid) at 100℃ on their quality characteristics. The highest stem heating loss was observed in the sodium chloride solution (S-NB) at 15.10% (p<0.001), and the highest leaf heating loss was in L-NB at 11.44% (p<0.001). No significant variation was found in the moisture content of both stems and leaves. Relative to the control, the L value (lightness) of the stem decreased while the b value (yellowness) increased (p<0.05). In the leaves, the a value (redness/greenness) and b value increased in L-CB, whereas they decreased in other groups (p<0.05). S-NB exhibited the highest true retention (TR) of total polyphenol content (TPC) in stems (p<0.01) and the highest TR of total flavonoid content (TFC) as well (p<0.001). For leaves, the highest TR of TPC and TFC was in L-WB (p<0.001). In terms of antioxidant activity, S-NB in stems and L-WB in leaves showed the highest scavenging activity measured by DPPH and ABTS+ assays (p<0.001). Microorganisms were absent in all pretreatment groups but present in the control. The results suggest that blanching pak choi stems in 2% NaCl solution and leaves in water optimally preserves biologically active compounds and nutrients.