• Korean Journal of Plant Tissue Culture
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• v.21 no.6
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• pp.327-332
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• 1994

The effect of methylglyoxal-bis (guanylhydrazone) (MGBG) and ethylene synthesis inhibitors on adventitious root formation from soybean cotyledon in relation to ethylene production and endogenous polyamine content was investigated. Cotyledon explants cultured on rooting medium formed numerous adventitious rook on the cut surfaces after 2 weeks of culture. However when cultured on rooting medium supplemented with MGBG, the root formation was strongly inhibited, its inhibitory effect was reserved when cultured on medium with MGBG + spermine, MGBG + CoCl$_2$ and MGBG + spermine+CoC1$_2$. A slight reversion of the rooting inhibition was observed in 10$^{-3}$ M MGBG +10$^{-5}$ M spermine treatment, whereas it caused a significant effect in 10$^{-3}$ M MGBG +10$^{-4}$ M treatment .Ethylene production and endogenous polymine content was investgated in 10$^{-3}$ M MGBG , 10$^{-3}$ M MGBG +10$^{-5}$ Mspermine, 10$^{-3}$ M MGBG +10$^{-4}$ M CoCl$_2$and 10$^{-3}$ M MGBG +10$^{-5}$ M spermine +10$^{-4}$ M CoCl$_2$treatments. Ethylene production highest in 10$^{-3}$ M MGBG +10$^{-5}$ M spermine treatment was higher than control. In 10$^{-3}$ M MGBG +10$^{-5}$ M spermine + 10$^{-4}$ M CoCl$_2$ treatment, ethilene production was lowest, whereas polyamine level was highest.

• YAKHAK HOEJI
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• v.54 no.6
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• pp.441-448
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• 2010

In present study, classification and quality control of Genus Polygonatum were developed using the isolated from Polygonati Odorati Rhizoma and Polygonati Rhizoma. 3 components were isolated from Butanol fractions of Polygonati Rhizoma, and 2 components were isolated from Hexane and Butanol fractions of Polygonati Odorati Rhizoma. All the components were obtained using silica gel and ODS column chromatography. The compounds were identified as adenosine, 14-hydroxylfurost-5-ene-3-O-${\beta}$-D-glucopyranosyl-($1{\rightarrow}2$)-O-${\beta}$-D-glucopyranosyl-($1{\rightarrow}4$)-O-${\beta}$-D-galactopyranosyl-26-O-${\beta}$-D-glucopyranoside, 22-O-methyl-14-hydrocxyfurost-5-ene-3-O-${\beta}$-D-glucopyranosyl-($1{\rightarrow}2$)-O-${\beta}$-D-glucopyranosyl-($1{\rightarrow}4$)-O-${\beta}$-Dgalactopyranosyl-26-O-${\beta}$-D-glucopyranoside, ${\beta}$-Sitosteryl-3-O-${\beta}$-D-D-glucopyranoside, 14-hydoxylfurost-5-ene-3-O-${\beta}$-Dglucopyranosyl-($1{\rightarrow}2$)-O-[${\beta}$-D-xylopyranosyl-($1{\rightarrow}3$)]-O-${\beta}$-D-glucopyranosyl-($1{\rightarrow}4$)-O-${\beta}$-D-galactopyranoside through physicochemical data, spectroscopic methods ($^1H$-NMR, $^{13}C$-NMR, Mass) according references. The quality control of genus Polygonatum were conducted using HPLC quantitative analysis of 14-hydroxylfurost-5-ene-3-O-${\beta}$-D-glucopyranosyl-($1{\rightarrow}2$)-O-${\beta}$-D-glucopyranosyl-($1{\beta}4$)-O-${\beta}$-D-galactopyranosyl-26-O-${\beta}$-D-glucopyranoside, 14-hydoxylfurost-5-ene-3-O-${\beta}$-D-glucopyranosyl-($1{\rightarrow}2$)-O-[${\beta}$-D-xylopyranosyl-($1{\rightarrow}3$)]-O-${\beta}$-D-glucopyranosyl-($1{\rightarrow}4$)-O-${\beta}$-D-galactopyranoside in 30 samples collected throughout Korea and China. This method provided a tool for standardization of mix or misusing the commercial Odorati Rhizoma and Polygonati Rhizoma. As a result, contained quantity of 14-hydroxylfurost-5-ene-3-O-${\beta}$-D-glucopyranosyl-($1{\rightarrow}2$)-O-${\beta}$-D-glucopyranosyl-($1{\rightarrow}4$)-O-${\beta}$-D-galactopyranosyl-26-O-${\beta}$-D-glucopyranoside was measured $0.008{\pm}0.006%$ and 14-hydoxylfurost-5-ene-3-O-${\beta}$-D-glucopyranosyl-($1{\rightarrow}2$)-O-[${\beta}$-D-xylopyranosyl-(13)]-O-${\beta}$-D-glucopyranosyl-($1{\rightarrow}4$)-O-${\beta}$-Dgalactopyranoside was measured $0.026{\pm}0.012%$.

• Korean Journal of Food Science and Technology
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• v.40 no.1
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• pp.42-46
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• 2008

The physical properties of ${\kappa}/{\lamda}$- and ${\kappa}/{\iota}$-carrageenan films, mixed with ${\lamda}$- or ${\iota}$-carrageenan (${\kappa}:{\lamda}$ or ${\kappa}:{\iota}$ = 2:1, 1.5:1.5, 1.2:1.8) and with polyethylene glycol, were compared to those of ${\kappa}$-, ${\lamda}$-, or ${\iota}$-carrageenan films. The tensile strengths of the ${\kappa}/{\lamda}$- and ${\kappa}/{\iota}$-carrageenan films decreased with increasing mixing ratios of ${\kappa}/{\lamda}$- or ${\kappa}/{\iota}$-carrageenan, respectively, and were lower than those of the ${\lamda}$- and ${\iota}$-carrageenan films. The elongations and water vapor permeabilities of the ${\kappa}/{\lamda}$- and ${\kappa}/{\iota}$-carrageenan films increased with increasing mixing ratios of ${\lamda}$- and ${\iota}$-carrageenan, respectively, and were higher than those of the ${\lamda}$- or ${\iota}$-carrageenan films.

• Journal of the Korea Society of Computer and Information
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• v.17 no.4
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• pp.139-145
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• 2012

Generally, Quicksort selects the pivot from leftmost, rightmost, middle, or random location in the array. This paper suggests Quicksort using middle range pivot $P_0$ and continually divides into 2. This method searches the minimum value $L$ and maximum value $H$ in the length n of list $A$. Then compute the initial pivot key $P_0=(H+L)/2$ and swaps $a[i]{\geq}P_0$,$a[j]<P_0$ until $i$=$j$ or $i$>$j$. After the swap, the length of list $A_0$ separates in two lists $a[1]{\leq}A_1{\leq}a[j]$ and $a[i]{\leq}A_2{\leq}a[n]$ and the pivot values are selected by $P_1=P_0/2$, $P_2=P_0+P_1$. This process repeated until the length of partial list is two. At the length of list is two and $a$[1]>$a$[2], swaps as $a[1]{\leftrightarrow}a[2]$. This method is simpler pivot key process than Quicksort and improved the worst-case computational complexity $O(n^2)$ to $O(n{\log}n)$.

• Korean journal of food and cookery science
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• v.14 no.1
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• pp.21-24
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• 1998

The purpose of this study was to evaluate the microbiological quality of kitchen utensils in institutional foodservices in Seoul. Total plate count of plastic container, knife, wiping clothes and cutting board are 1${\times}$$10^3$-1${\times}$10/sup/5(CFU/100 $cm^2$). There were many coliforms in plastic container (2${\times}$$10^1$CFU/100 $m^2$), knife (2-3${\times}$$10^1$CFU/100 $cm^2$), wiping clothes (4-6${\times}$$10^1$CFU/100 $cm^2$) and cutting board (4-9${\times}$$10^1$CFU/100 $cm^2$). The results of microbiological test of kitchen utensils indicated that the sanitary conditions of plastic container, knife, wiping clothes and cutting board should be improved promptly. Electron microscopic observation showed that there were too many bacteria in plastic containers.

• Korean Journal of Materials Research
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• v.5 no.6
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• pp.723-731
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• 1995

P $b_2$S $r_2$( $Y_{1-x}$ C $a_{x}$)C $u_3$ $O_{8+}$$\delta$/ samples were prepared with x=0.4~0.6 and small $\delta$. To minimize the extent of oxidative decomposition reaction which occurs during the preparation of this phase, two annealing steps were adopted : First, sintered samples of P $b_2$S $r_2$( $Y_{1-x}$ C $a_{x}$)C $u_3$ $O_{8+}$$\delta$/ are oxygenated under 100% $O_2$, which leads to a large $\delta$(e.g., $\delta$=1.8). Second, the resulting samples are deoxygenated under 0.1~1.0% $O_2$in $N_2$, lowering $\delta$ to desired values. This two-step annealing procedure minimized the extent of oxidative decomposition. However, even with the two-step annealing procedure, the oxidative decomposition of P $b_2$S $r_2$( $Y_{1-x}$ C $a_{x}$)C $u_3$ $O_{8+}$$\delta$/ cannot be completely suppressed if $\delta$ is to be reduced to maximize $T_{c}$. Electrical resistivity data show that $T_{c}$(onset) is a function of hole concentration in the Cu $O_2$layer, and the optimum hole concentration for the maximum $T_{c}$ is achieved when $Ca^{2+}$is substituted for $Y^{3+}$between 0.5 and 0.6 A $T_{c}$(onset)=80K has been observed for one such sample, and this is the highest $T_{c}$(onset) yet reported for this compound.ed for this compound.nd.

• Journal of the Korean Ceramic Society
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• v.40 no.9
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• pp.859-866
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• 2003

When calcium sulfoaluminate-based expansive cement was hydrated, ettringite and monosulfate were mainly formed. The crack of hardened cement was prevented by compensating drying shrinkage due to formation of the above hydrates. In order to study the hydration properties of calcium sulfoaluminate-based expanding cement, 3CaOㆍ3Al$_2$O$_3$ㆍCaSO$_4$(C$_4$A$_3$S) was prepared by chemical synthesis, and then the hydration of $C_4$A$_3$S-Ca(OH)$_2$-CaSO$_4$.$2H_2O$-C$_3$A system_was characterized. Good $C_4$A$_3$S phase was prepared at $1300^{\circ}C$ by chemical synthesis, and the main hydration product of $C_4$A$_3$S-Ca(OH)$_2$-CaSO$_4$.2$H_2O$ system was ettringite. In the case of hydration $C_4$A$_3$S-Ca(OH)$_2$-CaSO$_4$ㆍ 2$H_2O$-C$_3$A system, ettringite was formed in the early period and it was transformed into monosulfate while consumed gypsum.

• Korean Journal of Materials Research
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• v.12 no.7
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• pp.555-559
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• 2002

Blue-emitting $Sr_{10}$($PO$)$_{6}$ $Cl_2$:$Eu^{2+}$ and $_{(Sr,Mg) }$ 10/($PO_4$)$_{6}$ $Cl_2$:$Eu^{2+}$ phosphor particles for application of long-wavelength UV LED were prepared by ultrasonic spray pyrolysis. The luminescence characteristics under long- wave-length ultraviolet of the $Sr_{10}$ ($PO_4$)$_{6}$ $Cl_2$:$Eu^{2+}$ and (Sr,Mg)$_{10}$ ($PO_4$)$_{6}$ $Cl_2$:$^Eu{2+}$ phosphor particles prepared by the spray pyrolysis were compared with that of the commercial product. The PL intensity of the $Sr_{10}$ ($PO_4$)$_{6}$ $Cl_2$:$Eu^{2+}$ particles prepared by the spray pyrolysis was lower than that of the commercial $Sr_{10}$ ($PO_4$)$_{6}$ $Cl_2$:$Eu^{2+}$ particles because prepared $Sr_{10}$ ($PO_4$)$_{6}$ $Cl_2$:$Eu^{2+}$ phosphor particles had porous structure and hollow morphology. However, the PL intensity of the (Sr,Mg)$_{10}$($PO_4$)$_{6}$ $Cl_2$:$Eu^{2+}$ phosphor particles prepared by the spray pyrolysis was 8% higher than that of the commercial one. The high brightness of $(Sr,Mg)_{10}$ ($PO_4$)$_{6}$ $Cl_2$:Eu$^{2+}$ phosphor particles prepared by spray pyrolysis is due to the dense structure and high crystallinity of particles. The TEX>$(Sr,Mg)<_{10}$ ($PO_4$)$_{6}$ /$Cl_2$:$Eu^{ 2+}$ phosphor particles had main emission peak t 448 nm under long- wavelength ultraviolet.

• Journal of Korean Theatre Studies Association
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• no.53
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• pp.89-128
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• 2014

De notre temps, nous faisons face ${\grave{a}}$ un changement du $th{\hat{e}}atre$ dans son ensemble. Le $th{\acute{e}}atre$ contemporain tente de se $d{\acute{e}}marquer$ des autres formes artistiques, mais aussi des formes $litt{\acute{e}}raires$, lorsque la tendance est ${\grave{a}}$ la fusion des genres. Il existe ainsi de plus en plus de $th{\hat{e}}atre$ ${e}xp{\acute{e}}rimentaux$, $n{\acute{e}}s$ sous le nom de $th{\acute{e}}atre$ anthropologique, $th{\acute{e}}atre$ sociologique, $th{\acute{e}}atre$ ethnologique, $th{\acute{e}}atre$ ${\acute{e}}pist{\acute{e}}miologique$ ou $th{\acute{e}}{\hat{a}}tre$ interculturel. La $m{\acute{e}}thodologie$ doit elle s'adapter ${\grave{a}}$ cette tendance de fusion? Au lieu de trouver une $m{\acute{e}}thode$ d'anlyse de chaque "nouveaux genre" $n{\acute{e}}$ de la fusion, nous nous interrogeons sur la $m{\acute{e}}thodologie$ pour ${\acute{e}}tudier$ le $th{\hat{e}}atre$ dans son ensemble. Si une telle $m{\acute{e}}thodologie$ d'analyse existait, quelle serait la $diff{\acute{e}}rence$ avec la $m{\acute{e}}thodologie$ $d{\acute{e}}j{\grave{a}}$ existante? Et si elle n'existait pas, quelle serait la meilleure $m{\acute{e}}thodologie$ $adapt{\acute{e}}e$? Nous $consid{\acute{e}}rons$ la $m{\acute{e}}thode$ d'analyse de la didascalie comme nouvelle $m{\acute{e}}thodologie$ $adapt{\acute{e}}e$. En effet, la didascalie $conna{\hat{i}}t$ sans cesse des $m{\acute{e}}tamorphoses$ dans $l^{\prime}{\acute{e}}criture$. De nombreux $th{\acute{e}}oriciens$ ont $d{\acute{e}}velopp{\acute{e}}$ des $m{\acute{e}}thodes$ d'analyse afin de mieux comprendre les diverses formes de $th{\acute{e}}atre$ comtemporain et ses didascalies. Notre travail est de rassembler les ${\acute{e}}tudes$ des $diff{\acute{e}}rents$ $th{\acute{e}}oriciens$ et de les ordonner ; afin de proposer un outil d'analyse $adapt{\acute{e}}$ pour $l^{\prime}interpr{\acute{e}}tation$ des $pi{\grave{e}}ces$ du $th{\acute{e}}{\hat{a}}tre$ contemporain.

• Journal of Life Science
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• v.20 no.2
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• pp.260-268
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• 2010

The lactate dehydrogenase (EC 1.1.1.27, LDH) isozymes in tissues from Channa argus were purified and characterized by biochemical, immunochemical and kinetic methods. The activity of LDH in skeletal muscle was the highest at 380.4 units and those in heart, eye and brain tissues were 13.4, 3,5 and 5.4 units, respectively. Citrate synthase (EC 4.1.3.7, CS) activity in heart tissue was the highest at 20.7 units. LDH/CS in skeletal muscle, heart, eye and brain tissues were 172.9, 0.6, 0.32 and 0.47. Protein concentration in skeletal muscle tissue was 14.7 mg/g and specific activities of LDH in skeletal muscle, heart, eye and brain tissues were 25.88, 0.79, 0.31 and 1.38 units/mg, respectively. Therefore, skeletal muscle tissue was anaerobic and heart tissue was aerobic. The LDH isozymes in tissues were identified by polyacrylamide gel electrophoresis, immunoprecipitation and Western blot with antiserum against $A_4$, $B_4$, and eye-specific $C_4$. LDH $A_4$, $A_3B$, $A_2B_2$. $AB_3$ and $B_4$ isozymes were detected in every tissue, $C_4$, $AC_3$, $A_2C_2$ and $A_3C$ were detected in eye tissue, and $A_3C$ was found in brain tissue. LDH $A_4$, $A_3B$, $A_2B_2$, $AB_3$, $B_4$, eye-specific $C_4$ isozymes were purified by affinity chromatography and Preparative PAGE Cells. The LDH $A_4$ isozyme was purified in the fraction from elution with $NAD^+$ containing buffer of affinity chromatography. Eye-specific $C_4$ isozyme was eluted right after $A_4$, after which $B_4$ isozyme was eluted with plain buffer. As a result, one part of molecular structures in $A_4$, $B_4$ and eye-specific $C_4$ were similar, but were different from each other in $B_4$ and $C_4$. Therefore the subunit A may be conservative in evolution, and the evolution of subunit B seems to be faster than that of subunit A. The activity of LDH $A_4$, $A_2B_2$, $B_4$, and eye-specific $C_4$ isozymes remained at 39.98, 21.28, 19.67 and 16.87% as a result of the inhibition by 10 mM of pyruvate, so the degree of inhibition was very high. The $Km^{PYR}$ values were 0.17, 0.27 and 0.133 mM in $A_4$, $B_4$ and eye-specific $C_4$ isozymes, respectively. The optimum pH of LDH $A_4$, $B_4$, eye-specific $C_4$, $A_2B_2$, $A_3B$, and $AB_3$ were pH 6.5, pH 8.5, pH 5.5, pH 6.0-6.5, pH 5.0 and pH 7.5. The $A_4$ and heterotetramer isozymes stabilized a broad range of pH. Especially, LDH activities in skeletal muscle tissue were high, resulting in a high degree of muscle activity.LDH metabolism in eye tissue seems to be converted faster from pyruvate to lactate by eye-specific $C_4$ isozyme as eye-specific $C_4$ have the highest affinity for pyruvate, and right after the conversion, oxidation of lactate was induced by $A_4$ isozyme. It was found that expression of Ldh-C, affinity to substrate and reaction time of $C_4$ isozyme were different according to the ecological environmental and feeding capturing patterns.