• Microbiology and Biotechnology Letters
• /
• v.47 no.2
• /
• pp.259-268
• /
• 2019

In this study, the extracellular metalloprotease from Serratia marcescens PPB-26 was purified to homogeneity via ethanol fractionation and DEAE-cellulose column chromatography. Thus, a 3.8-fold purification was achieved with a 20% yield and specific activity of 76.2 U/mg. The purified protease was a 50-kDa monomer whose optimum pH and temperature for activity were 7.5 and $30^{\circ}C$ respectively; however, it was found to remain active in the 5-9 pH range and up to $40^{\circ}C$ for 6 h. The protease had a half-life of 15 days at $4^{\circ}C$, an optimum reaction time of 10 min, and an optimum substrate (casein) concentration of 0.25%. Furthermore, the Michaelis constant ($K_m$) and reaction velocity ($V_{max}$) of the protease were calculated to be 0.28% and $111.11{\mu}moles/(min{\cdot}mg)^{-1}$, respectively. The protease was stable when subjected to metal ions (2 mM), showing increased activity with most (especially $CoCl_2$ and $MgSO_4$ (30.54% increase)). It was also stable when exposed to oxidizing agents, bleaching agents, and detergents (5% v/v for 60 min). It retained 93% of its activity in non-ionic detergents (Tween-20, Tween-80, and Triton X-100). Moreover, wash performance analysis in commercial detergents (Ariel and Tide) showed that not only was the protease capable of protein stain removal, but also reduced cleaning time by 80% when added to detergents. Thus, the Serratia marcescens PPB-26 metalloprotease appears to be a promising new candidate as a laundry additive in the detergent industry.

• Proceedings of the Korea Water Resources Association Conference
• /
• 2019.05a
• /
• pp.271-271
• /
• 2019

하천에서의 식생은 하천의 조도 및 흐름특성을 변화시키는 요인 중 하나로서 하천 내의 식생밀도 변화로 인하여 발생한 하상변동은 장기적으로 하천내의 하중도가 발생하거나, 식생으로 인해한 방향으로 편중된 흐름으로 인하여 제내지 방향으로의 하천 침식이 발생하고, 이에 따라 구조물의 안정성에 영향을 미치는 등 하천의 중 장기 계획 시 고려해야할 중요한 요인이다. 이에 따라 식생 모형을 이용한 다양한 흐름특성 분석 실험들이 수행되었으나, 대부분의 연구에서는 식생을 단순화하여 모형으로 사용하였기 때문에 실제 식생에 대한 영향성을 보기에는 한계가 존재한다. 건설기술연구원의 하천실증연구센터에서는 이러한 식생의 영향을 분석하기 위하여 네덜란드의 Deltares, 핀란드의 Aalto 대학 등과 함께 다년간 국제공동연구를 수행하였으며, 금번 소개하는 실험에서는 식생밀도에 따른 흐름변화, 하상변동 및 환경 변화 등의 검토를 목적으로 하였다. 실험은 총 2가지 유량 조건에 대하여 수행되었으며, 실험 중 흐름의 안정화를 확인하기 위하여, 유출수조에는 6개의 압력식 수위계를 설치하여 실험기간동안 유출수조에서의 수심변화를 측정하였고, 하도에는 실험구간 상 하류에 SonTek의 SL-1500 및 SL-3000을 이용하여 지속적으로 수심을 측정하고, 이 외에도 하류단에 2개의 압력식 수위 측정장비를 설치하여 수심을 모니터링하였다. 실험에 사용된 식생은 높이 약 1.5m의 2가지 밀도로 폭 2m, 길이 4m의 규모로 설치하였고, 광파기와 3차원 레이저 스캐너를 이용하여 실험 전 후의 식생 주변 및 전 실험구간의 하상을 측량하였다. 흐름특성 분석을 위한 수리 측정은 Nortek사의 Vectrino를 이용하여 측정하였고, 총 24개 측선에 대하여 각 측선별 5 ~ 10개의 유속자료를 측정하였으며, 각 측점별로 90초간 자료를 수집하였다. 흐름특성 분석에 사용된 유속자료는 이상치를 제거한 후 수행되었으며, 이상치의 제거는 표준편차의 3배 이상의 편차를 갖거나, 측정자료의 자기상관도가 70% 이하인 값들은 제거한 후 분석을 수행하였다. 흐름 분석을 위한 측정자료를 이 외에도 캠코더를 이용한 표면유속영상 측정기법과 ADCP를 이용한 측정도 병행하였으나, 본 연구에서는 Vectrino로 측정된 결과만을 소개하고자 한다.

• Journal of the Korean Wood Science and Technology
• /
• v.30 no.3
• /
• pp.1-11
• /
• 2002

This study was conducted to investigate the effects of reaction pH conditions and hardener types on the reactivity, chemical structure and adhesion performance of UF resins. Three different reaction pH conditions, such as traditional alkaline-acid (7.5 → 4.5), weak acid (4.5), and strong acid (1.0), were used to synthesize UF resins which were cured by adding three different hardeners (ammonium chloride, ammonium citrate, and zinc nitrate) to measure adhesion strength. Fourier transform infrared (FT-IR) and carbon-13 nuclear magnetic resonance (¹³C-NMR) spectroscopies were employed to study chemical structure of the resin prepared under three different reaction pH conditions. Adhesion strength of the resins cured with three different hardeners was determined with lap shear specimens in tension. The gel time of UF resins decreased with an increasing in the amount of both ammonium chloride and ammonium citrate added in the resins. However, the gel time increased for zinc nitrate. Both FT-IR and ¹³C-NMR spectroscopies showed that the strong reaction pH condition produce uronic structures in UF resin, while both alkaline-acid and weak acid conditions produce quite similar chemical species in the resins. The maximum adhesion strength was occurred with the resin prepared under strong acid pH condition. However, this study indicated that the weak acid reaction condition provide a balance between increasing resin reactivity and improving adhesion strength of UF resin. The measurement of formaldehyde emission from the panels bonded with the UF resins prepared is planned for future work.

• Journal of the Korean Institute of Gas
• /
• v.27 no.2
• /
• pp.49-56
• /
• 2023

As AI (Artificial Intelligence)-related technologies are highly developed due to the 4th industrial revolution, cases of applying AI in various fields are increasing. The main reason is that there are practical limits to direct processing and analysis of exponentially increasing data as information and communication technology develops, and the risk of human error can be reduced by applying new technologies. In this study, after collecting the data received from the 'remote potential measurement terminal (T/B, Test Box)' and the output of the 'remote rectifier' at that time, AI was trained. AI learning data was obtained through data augmentation through regression analysis of the initially collected data, and the learning model applied the value-based Q-Learning model among deep reinforcement learning (DRL) algorithms. did The AI that has completed data learning is put into the actual city gas supply area, and based on the received remote T/B data, it is verified that the AI responds appropriately, and through this, AI can be used as a suitable means for electricity management in the future. want to verify.

• The Journal of the Institute of Internet, Broadcasting and Communication
• /
• v.22 no.6
• /
• pp.21-30
• /
• 2022

This paper describes the design, manufacture, and testing of servos applicable to small tracking radars. First, Chapter 1 describes the necessity of this study. Chapter 2 describes the development of servos applicable to future tracking radars in small missile systems. Chapter 3 describes the design and test results for current control of brushed DC motors, brushless DC motors, and permanent magnet synchronous motors. And Chapter 4 describes the design and test results for speed control of the test wheel. And in Chapter 5, the results of the previous tests are summarized. In this paper, some pictures were intentionally blurred for security reasons, and the control result of test wheel was described, not the test with the developed gimbals.

• Proceedings of the Korea Water Resources Association Conference
• /
• 2022.05a
• /
• pp.264-264
• /
• 2022

하천의 합류부는 두 개 이상의 하천이 하나로 합쳐지는 구간으로 서로 다른 특성으로 인해 급격한 흐름의 변화와 수리학적 지형변화가 발생하는 구간이다. 하천의 합류부에서는 유체의 물리화학적인 특성과 흐름 구조의 변화가 발생할 수 있다. 흐름 구조의 변화로 인한 유사 이송으로 세굴과 같은 지형적인 변화가 발생할 수 있다. 합류부의 혼합을 이해하기 위해서는 본류와 지류의 다양한 유입조건에 따른 공간적인 패턴을 분석하는 것이 중요하다. 그러나, 대부분의 합류부 연구들은 실측에 기반한 공간적인 패턴 분석의 어려움으로 인해 실내실험 또는 수치모형에 의존하여 연구가 수행되어, 실측자료에 기반한 공간적인 수체혼합의 분석은 매우 제한적이었다. 따라서, 본 연구에서는 하천 합류부의 혼합 현상을 규명하는 인자로 흐름 방향 유속, 2차류와 수심 등 기본적인 수리학적 인자들 외에 연직, 수평 방향으로 측정한 수질 자료와 드론 영상을 활용하여 합류부의 혼합 특성을 해석하고자 하였다. 수질 자료 중 하천의 혼합을 가장 잘 확인할 수 있는 인자로써 전기전도도와 온도를 활용하였다. SonTek ADCP를 이동식으로 횡단하여 측정해 흐름 방향 유속과 2차류, 수심을 확인하였다. ADCP를 운용함과 동시에 YSI의 수질센서를 활용하여 연직, 수평 방향으로의 전기전도도와 온도의 분포를 확인하였다. 또한, 합류부의 2차원 공간적인 분포를 확인하기 위해 드론 영상을 촬영하였다. ADCP, YSI, 드론의 계측자료는 한국의 낙동강과 남강 합류부에서 측정되었고, 분석 결과 계측장비 간의 경향성이 일치하였다. 또한, 이전에 진행된 해외의 합류부 연구 결과와 유사한 결과가 관측되었으나, 일부 부분에서는 다른 결과를 보였다.

• Journal of the Korea Concrete Institute
• /
• v.24 no.4
• /
• pp.453-463
• /
• 2012

Steel-concrete composite rigid-frame bridge is a type of integral bridge having advantages in bridge maintenance and structural efficiency from eliminating expansion joints and bridge supports, the main problems in bridge maintenance. The typical steel-concrete composite rigid-frame bridge has the girder-abutment connection where a part of its steel girder is embedded in abutment for integrity. However, the detail of typical girder-abutment connection is complex and increases the construction cost, especially when a part of steel girder is embedded. Recently, a new type of bridge was proposed to compensate for the disadvantages of complex details and cost increase. The compensation are expected to improve efficiency of construction by simplifying the construction detail of the girder-abutment connection. In this study, a static load test has been carried out to examine the behavior of the girder-abutment connection using real-scale specimens. The results of the test showed that the girder-abutment connection of proposed girder bridge has sufficient flexural capacity and rebars to control concrete crack should be placed on the top of abutment.

• Korean Journal of Health Education and Promotion
• /
• v.32 no.2
• /
• pp.1-13
• /
• 2015

Objectives: This study was conducted to investigate derived factors associated with a public service announcement on handwashing to evaluate the effect of this announcement. Methods: In early september, 1,000 persons over 19 years old was investigated by Computer Aided Telephone Interview, at last 993 persons for analysis. Outcomes were the exposure to the public service announcements on handwashing, the comprehension of this announcements, the intention of behavioral change and the practice of handwashing. Results: In particular, the proportion of exposure is more women and highly educated. The comprehension of the announcement was no statistically significant difference. The intention of behavior change showed higher in workers and understanding of the announcements. The practice of handwashing is higher in women and lower in student/unemployed. There was no difference in practice in accordance with the exposure, the comprehension and the intention of behavioral change. Conclusions: About the comprehension of the announcements and the intention of behavioral change, the effects of the public service announcements on handwashing can be very positive. But it does not affect the actual practice of hand washing. The handwashing promotion programs will have to devise a strategy and structure, operation method considering realistic and scalable platform.

• Archives of design research
• /
• v.20 no.3 s.71
• /
• pp.119-128
• /
• 2007

With the development of digital technologies, CAID became an essential part in the industrial design process. Creating photo-realistic images from a virtual scene with 3D models is one of the specialized task for CAID users. This task requires a complex interface of setting the positions and the parameters of camera and lights for optimal rendering results. However, the user interface of existing CAID tools are not simple for designers because the task is mostly accomplished in a parameter setting dialogue window. This research address this interface issues, in particular the issues related to lighting, by developing and evaluating TLS(Tangible Lighting Studio) that uses Augmented Reality and Tangible User Interface. The interface of positioning objects and setting parameters become tangible and distributed in the workspace to support more intuitive rendering task. TLS consists of markers, and physical controller, and a see-through HMD(Head Mounted Display). The user can directly control the lighting parameters in the AR workspace. In the evaluation experiment, TLS provide higher effectiveness, efficiency and user satisfaction compared to existing GUI(Graphic User Interface) method. It is expected that the application of TLS can be expanded to photography education and architecture simulation.

• Archives of design research
• /
• v.18 no.4 s.62
• /
• pp.119-128
• /
• 2005

This paper presents an intuitive 3D modeling interlace based on a field study and prototype development. The process and tools of modeling were observed in workshops of professional design model making, day modeling, wood caning and glass crafting. The Spray Modeling interlace was developed from the observational analysis of the field study. It is a 3D modeling interface which combines particle spraying and day modeling in Virtual or Augmented Reality space. Virtual volume particles are sprayed on frames in Augmented Reality space as day modeling. It adopts a real air spay gun as a tangible interface device which provides coherent sound and air-force feedback. The prototype development and a user study showed that the interface supports new patterns of form development and expression. Control interfaces and requirements of auxiliary devices were found to be improved. This study examines the potential of the new interlace for designers working in 3D virtual and augmented reality. The new spraying interface is also expected to be used as an alternative interface in 3D computer workspace, games, education software and media art.