• The Korean Journal of Nuclear Medicine
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• v.26 no.2
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• pp.346-354
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• 1992

Cancer cells have several tumor-associated antigens on the cell surfaces, and antibodies against these antigens have been developed by many investigators. Radiolabeled antibodies have been used as new methods to diagnose and treat malignant tumors. Especially anti-carcinoembryonic antigen (CEA) is the most popular antibody for these purposes. In this investigation, we tried to label $^{131}I$ and $^{99m}Tc $ to anti-CEA monoclonal antibodies which were developed in the Seoul National University College of Medicine. We found CEA-79 and CEA-92 antibodies had the better immunological characteristics among 8 anti-CEA monoclonal antibodies. And radioiodination of CEA-79 could be performed by chloramine-T method, while radioiodination of CEA-92 by iodogen method. To label these antibodies with $^{99m}Tc $, we used pretargeting transchelation as direct labeling method. At first, $^{99m}Tc $ was bound to glucaric acid, and monoclonal antibody was reduced by $\beta-mercaptoethanol$. When these were incubated together. $^{99m}Tc $ bound to glucarate was switched to monoclonal antibody because of higher affinity. We established conditions of several steps in this method. Anti-CEA monoclonal antibodies labeled with $^{131}I$ and $^{99m}Tc $ are expected to be used valuably in the detection and treatment of malignant tumors.

• The Korean Journal of Nuclear Medicine
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• v.26 no.2
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• pp.274-279
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• 1992

Clinical role of $^{99m}Tc-MIBI$ myocardial scintigraphy in the diagnosis of coronary artery disease (CAD) is now well accepted, however, the role of it in the identification of viable myocardium in patients with chronic CAD has not yet been clarified. To determine the usefulness of rest-injected $^{99m}Tc-MIBI$ scan as a marker of myocardial viability, the regional uptake of this agent at rest was compared with that of $^{201}Tl$ on reinjection and 24 hours after reinjection images. Subject patients were 13 chronic CAD patients who showed irreversible perfusion defect(s) on standard pharmacologic (dipyridamole) stress-redistribution images. Immediately after the redistribution images were obtained, 37 MBq thallium was injected at rest, and images were reacquired at 10 minutes and 24 hours after reinjection. After then 740 MBq $^{99m}Tc-MIBI$ was injected, and 1 hour later rest MIBI myocardial imaging was performed. Five sets of imagestress, redistribution, reinjection, delayed images of thallium, and rest image of MIBI) were then analyzed qualitatively and quantitatively. Left ventricle was arbitrarily divided into 9 segments (apex, basal and apical portions of anterior, septal, inferior, and lateral walls). Seven patients and 30 regions showed a fixed perfusion defect on the stress-redistribution images. Among 30 regions, 15 showed positive uptakes and 6 showed negative uptakes on both $^{201}Tl$ reinjection/delayed images and $^{99m}Tc-MIBI$ rest images. Five regions showed only thallium uptake and were regarded as viable clinically. Of four regions which showed only $^{99m}Tc-MIBI$ uptake, two were regarded as viable, while the other two were regarded as a nonviable scar tissue clinically. In conclusion, $^{201}Tl$ reinjection technique was more reliable in the identification of viable myocardium. However, the role of $^{99m}Tc-MIBI$ in identification of viable myocardium was still remained to be clarified because 2 of 9 regions showed only $^{99m}Tc-MIBI$ uptake and were regarded as viable tissues.

• Advances in Traditional Medicine
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• v.9 no.1
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• pp.58-66
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• 2009

Allergic conjunctivitis is the most common allergic disease. These diseases are severe & frequent which requires search of new treatments. The aim of the study was to investigate the effects of Tinospora cordifolia (TC), Rubia cordifolia Linn. (RC) on experimentally induced allergic conjunctivitis in rats. In this study, dried water soluble extracts of TC and RC. (250 and 500 mg/kg, p.o. for 7 days) were evaluated for their antiallergic activity in Wistar rats. They were tested for inhibition of egg albumin-induced vascular permeability, inhibition of histamine release from the rat conjunctiva as well as in histamine content in tears. TC and RC showed significant (P < 0.05) inhibition in vascular permeability, inhibition in histamine release from the rat conjunctiva which is reflected by reduced level of histamine content in tears. The activities were found to be comparable to azelastine hydrochloride. These results suggest that the inhibitory effect on egg albumin-induced experimental allergic conjunctivitis in rat may be due to the antihistaminic activity of TC and RC. Our studies provide evidence that TC and RC may be beneficial in the treatment of allergic conjunctivitis.

• Bulletin of the Korean Chemical Society
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• v.27 no.8
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• pp.1189-1193
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• 2006

In order to develop radiopharmaceuticals for targeting a serotonin receptor such as $5-HT_{1A}$, histidine-$C_n$-arylpiperazines (AP) (C = alkyl, n = 2, 3, 4) were prepared in five steps with yields of 25%, 37% and 51%, respectively, and radiolabeled with the $[^{99m}Tc(CO)_3(H_2O)+3]^+$. The $^{99m}Tc(CO)_3$-Histidine-Cn-APs were prepared with a high yield (>99%) and characterized as a tridentate complex with a neutral charge to pass through the blood-brain barrier (BBB). The rhenium complexes with $Re(CO)_3$ were also synthesised and comparative experiments were achieved to evaluate the nature of the $^{99m}Tc$ complexes.

• Journal of Microbiology and Biotechnology
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• v.16 no.2
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• pp.205-211
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• 2006

A quantitative analytical method to detect new lines of genetically modified (GM) maize, NK603 and TC1507, has been developed by using a real-time polymerase chain reaction (PCR). To detect these GM lines, two specific primer pairs and probes were designed. A plasmid as a reference molecule was constructed from an endogenous DNA sequence of maize, a universal sequence of a cauliflower mosaic virus (CaMV) 35S promoter used in most GMOs, and each DNA sequence specific to the NK603 and TC1507 lines. For the validation of this method, the test samples of 0, 0.1, 0.5, 1.0, 3.0, 5.0, and 10.0% each of the NK603 and TC1507 GM maize were quantitated. At the 3.0% level, the biases (mean vs. true value) for the NK603 and TC1507 lines were 3.3% and 15.7%, respectively, and their relative standard deviations were 7.2% and 5.5%, respectively. These results indicate that the PCR method developed in this study can be used to quantitatively detect the NK603 and TC1507 lines of GM maize.

• Journal of the Society of Disaster Information
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• v.11 no.2
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• pp.287-296
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• 2015

In this study, international standards in disaster management and the management of the relevant International Standards Organizations(ISO), in particular ISO / TC 292 (Security- Safety) research subcommittee to look at international standards of relevant international standards for disaster management trends, each international domestic law related to the standard were researched. National Disaster Management Authority as a Ministry of Public Safety and Security (formerly the National Emergency Management Agency) has continued to prepare a plan for applying national disaster management relevant to the international standards. As the disaster management international standard continues to be established, disaster management agencies need to continue to keep pace with the international community to find ways to introduce international standards.

• International Journal of Fluid Machinery and Systems
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• v.8 no.1
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• pp.23-35
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• 2015

This paper presents the rotordynamic performance measurement of oil-free turbocharger (TC) supported on gas foil bearings (GFBs) for 2 liter class diesel vehicles and comparison to floating ring bearings (FRBs). Oil-free TC was designed and developed via the rotordynamic analyses using dynamic force coefficients from GFB analyses. The rotordynamics and performance of the oil-free TC was measured up to 85 krpm while being driven by a diesel vehicle engine, and compared to a commercial oil-lubricated TC supported on FRBs. The test results showed that the GFBs increased the rotor speed by ~ 20% at engine speeds of 1,500 rpm and 1,750 rpm, yielding the reduction of turbine input energy by more than 400 W. Incidentally, an external shock test on the oil-free TC casing was conducted at the rotor speed of 60 krpm, and showed a good capability of vibration damping due to the well-known dry friction mechanism of the GFBs.

• Transactions of the Korean hydrogen and new energy society
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• v.27 no.3
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• pp.245-255
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• 2016

Commercial production line of FCEV was built in 2013 by Hyundai and followed by Toyota in 2014, with additional manufacturers planning to enter the market around 2017. Now there is limited hydrogen infrastructure over the world, but more hydrogen stations are planned, particularly in Japan, Korea, Germany, UK and USA. ISO TC 197 which was created in 1990 focused standardization in the field of systems and devices for the production, storage, transport, measurement and use of hydrogen. Total number of published ISO standards related to the TC and its SCs (number includes updates) is 17. Now there are 20 participating countries and 13 observing countries. The current status of activity of ISO TC197 was reviewed and particularly issues on hydrogen quality and hydrogen refueling station were discussed.

• Journal of the Korean Society of Food Science and Nutrition
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• v.33 no.6
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• pp.1017-1021
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• 2004

To isolate a mutant which overproduces threonine and tryptophan, mutants of Saccharomyces cerevisiae were screened after UV and EMS mutagenesis. Hydroxynorvaline, a Thr analogue was used for selection of a Thr-overproducing mutant after UV mutagenesis. Among 31 mutants, TC 5-1 was selected as the strain candidate, based on amino acid analysis. TC 5-1 was then treated by EMS mutagenesis for Trp overproduction. Eight mutants were selected using fluorotryptophan for Thr and Trp overproducing strains. Amino acid analysis results showed that TC 6-1 was the best strain since it had the highest amount of Thr and Trp among mutants.

• The Korean Journal of Food And Nutrition
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• v.32 no.2
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• pp.133-137
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• 2019

Curcumin is a hydrophobic polyphenol extracted from turmeric that exhibits a variety of biological functions has albeit with limited efficacy as a functional food material owing to its low absorption when administered orally. The newly developed curcumin powder formulation exhibits improved absorption rate in vivo. This study evaluates the anti-oxidant effects of $Theracurmin^{(R)}$ (TC), which is highly bio-available in curcumin powder. The antioxidant activity of TC was investigated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity, ferrous reducing antioxidant power (FRAP) assays, NO radical, superoxide radical, $H_2O_2$ scavenging activity, and total antioxidant capacity (TAC). Additionally, we evaluated the antioxidant activity of TC in high-fat diet (HFD)-fed streptozotocin (STZ)-induced Type 2 diabetic rats. As a result of oral administration of TC for 13 weeks in type 2 diabetic rats, the group administration of 2,000 mg/kg significantly increased FRAP, superoxide dismutase (SOD), and reduced the level of glutathione (GSH) in liver tissue 1.9, 1.2, and 1.2-times, respectively. Furthermore, serum TAC levels increased by 1.3-fold after the rats were administered with a dose of 500 mg/kg. These results were consistent with the in vitro assay results. In conclusion, TC exhibited its potential as a functional food material through its antioxidant properties.