• 제목/요약/키워드: TB10.4

검색결과 373건 처리시간 0.029초

흉부 X-선 검사를 통한 노숙인 폐결핵 감염률 현황조사 (Survey on the Incidence of Homeless Pulmonary Tuberculosis Infection Rate through Chest X-ray Examination)

  • 김미영;신성례;류영환;임환열
    • 방사선산업학회지
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    • 제10권4호
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    • pp.249-255
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    • 2016
  • This study, Seoul City shelter, you are trying to seek medical cooperation and cure rate increase proposal Yu findings's current situation and tuberculosis of homeless tuberculosis. Inspector, and has a total 591 people is targeted to implement an interview after acquiring utilization agreement in studies conducted chest X-ray photography. Of the interview questions, three or more protons, it is determined that the TB symptomatic conducted sputum examination, chest X-ray examination confirms the physician radiology, when sputum examination primarily chromatic findings the double implemented and conducted by requesting the ship inspection also said inspection sputum acid-fast bacteria if it is true one, respectively. confirmed case result of checking whether there is a difference due to risk factors(Jb) at the chi square black, it was found that there is no statistically significant difference at 95% confidence level. (${\chi}^2=0.276$, p>0.05), suspected case (Ac, Ae) results of examining whether there is a difference due to risk factors in chi square black, that there is a statistically significant difference at 99% confidence level is I found (${\chi}^2=9.414$, p<0.01). The nature of the homeless tuberculosis screening and directed to the distance homeless specific location are likely to evaluate the actual incidence low and aggressive or management needs, the rationale is allowed insufficient reality is. Through this research, future, for tuberculosis high risk tuberculosis patient, such as homeless to expand the tuberculosis screening of infectious tuberculosis patients in private medical institutions, and one-stop service that chest X-ray examination and sputum examination is carried out at the same time introduced immediately to prevent the inspection and examination, cure, and should establish a foundation that can be up to post administration.

무거운 란탄족 이온 착물의 전기화학적 연구 (Electrochemical Studies on the Heavy Lanthanide Complexes)

  • 박종민;김재균;손병찬
    • 분석과학
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    • 제5권3호
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    • pp.249-261
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    • 1992
  • 무거운 란탄족 이온($Gd^{3+}$, $Tb^{3+}$, $Dy^{3+}$, $Ho^{3+}$, $Er^{3+}$, $Tm^{3+}$, $Yb^{3+}$$Lu^{3+}$)과 alixarin red S(ARS) 사이에 생성한 착물의 전기화학적 거동을 직류폴라로그래피, 펄스차폴라로그래피 및 순환전압전류법으로 연구하였다. 0.1M LiCl 지지전해질 용액에서 착화제인 ARS는 2전자 1단계의 가역성환원반응으로 $30^{\circ}C$까지는 확산 지배적이었으며 전극반응에서 흡착이 거의 일어나지 않았다. 란탄족 이온과 ARS는 1:3 착물을 형성하였으며 이 착물의 환원파는 펄스차이폴라로그래피 및 순환전압전류법으로 흡착성 착물파임을 확인하였다. 착물파의 환원전위는 ARS의 환원파보다 음전위에서 나타났으며 란탄족이온의 농도의 증가에 따른 ARS의 봉우리전류($P_1$)의 감소와 착물의 봉우리전류($P_2$)의 증가는 pH=9.5, 0.1M LiCl 및 ARS의 농도 $1{\times}10^{-3}M$일 때 란탄족 이온의 농도 $2{\times}10^{-6}M{\sim}6.4{\times}10^{-5}M$ 범위에서 직선적으로 변화하였다.

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오메가 계열 지방산 급여가 산란노폐계육의 지방산 조성 및 저장성에 미치는 영향 (Effects of Dietary $\omega$-Fatty Acids on Fatty Acids Composition and Storage Characteristics of Meats from Spent Hens)

  • 박구부;김진형;이한기;김영직;김용곤;이정일;박태선;정태철
    • 한국가금학회지
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    • 제24권2호
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    • pp.73-83
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    • 1997
  • This study was conducted to investigate the influence of dietary $\omega$-fatty acids on fatty acids composition and storage characteristics in meat samples of spent hens. Spent hens were randomly assigned to one of the four dietary treatments : 1) Control (commercial feed), 2) T1 (commercial feed supplemented with 10% olive oil, 3) T2 (commercial feed with 10% canola oil), and 4) T3 (commercial feed with 10% sardine oil). They were fed one of the experimental diets for three weeks and slaughtered. The meat samples were stored at 4 ${\pm}$ 1 ˚C The storage characteristics and fatty acid contents were analyzed for meat samples stored over a period of 1, 3, 5, 7 and 9 days. The pH of all treatments significantly increased during the storage periods(P<0.05). The per-oxide values (POV) of all treatments were significantly increased up to 5 days ; after that the POV decreased significantly (P<0.05). The POV of treated groups were significantly higher than those of the Control in the thigh meat(P<0.05). The TBARS showed the highest POV among all treatments. Although the breast meat tended to he lower in POV than the thigh meat, no significant difference was detected between the two meats. The TBARS(thiobarbituric acid reactive substances) of all treatments were significantly increased as the storage period extended(P<0.05). After 3 days, the TB ARS of oil-treated groups were aignificantly higher than that of the Control (P<0.05). The T3 showed the highest TBARS among all treatments (P<0.05). The TBARS of the breast meat was lower than that of the thigh, but no statistical difference was found be- tween them. The unsaturated fatty acid content of the breast and thigh meats in all treatments were slightly decreased as the storage period extended. The oleic acid was higher in Ti, and the linoleic acid and linolenic acid were higher in T2 than the other treatments. The eicosapentaenoic acid and docosahexaenoic acid were higher in T3 than the other treatments. The unsaturated fatty acid contents of the breast meat were slightly lower than those of the thigh meat. The n-6 fatty acid contents of the breast and thigh meats were slightly increased as the storage periods ex-tended. The n-3 fatty acid content of T3 was the highest among all treatments. The n-6 fatty acid content of breast meat was lower than that of thigh meat. The n-6 fatty acid content of the breast meat was slightly lower than that of the thigh meat.

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점적관개에서 관개율이 Sandy Loam토양의 습윤양상에 미치는 영향 (Effects of the irrigation Rate on Wetted Patterns in Sandy Loam Soil Under Trickle irrigation Condition)

  • 김철수;이근후
    • 한국농공학회지
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    • 제31권2호
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    • pp.104-115
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    • 1989
  • In an effort to clarify the wetted patterns of sandy loam soil under trickle irrigation conditions, the distance of wetted zone, infiltration capacity and soil wetted patterns, etc. were measured by gypsum block as soil moisture sensor located every 5 cm vertically and horizontaly in the soil bin under the such conditions as a). irrigation rates set to 2, 4, 6, 8 liters per hour b). total amount of water applied fixed to 14.62 liters per soil bin c) the hearing force of soil measured by plate penetrometer ranging from 1.04 to 1.22kg/cm$_2$ The results can be summarized as follows ; 1. The wetted distance in horizontal direction(H), the wetted distance in vertical direction(D), the horizontal infiltration capacity (iH) and the vertical infiltration capacity(in)could by explained as a function of time t. 2. The horizontal wetted distance (H) is explained by an exponetial function H= a$.$ t where b was found ranging from 021 to 026 under surface trickle irrigation, which was considered a lotlower than the classical value of 0.5 and these measurements were indifferent to the increasing irrigation rates. 3. As for the surface trickle irrigation where horizontal infiltration capacity(iH) is explained as iH = A $.$ t h, the coefficient A increases with respect to irrigation rates within the limits of 0.89~1.34. 4. In terms of surface trickle irrigation of the ratio of Dm Which is maximum vertical wetted distance to Hm, which is maximum horizontal wetted distance, found to be within range of 1.0 to 1.21. It was also noted that the value of Dm decreses when irrigation rates increases while the value of Hm changes the opposite direction. 5. The optimum location of sensors from emitter for surface trickle irrigation should he inside of hemisphere whose lateral radius is 28~30cm long and vertical radius is 10~12cm long. The distance between emitters should be within 60cm long. 6. In the study of vertical wetted distance( D) where D= a $.$ tb, the exponential coefficient b ranged from 0.61 to 0.75 in surface trickle irrigation, and from 0A9 to 0.68 for subsurface trickle irrigation. These measurements showed an increasing tendency to with respect to irrigation rates. 7. In case of vertical infiltration capacity( in), where iD= A $.$ t 1-h, the coefficient A for surface trickle irrigation found to be within range of 0.16 to 0.19 and did not show any relationships with varying degree of irrigation rates. However, the coefficient was varying from 0.09 to 0.22 and showed a tendency to increase vis-a-vis irrigation rates for subsurface trickle irrigation, in contrast. 8. In the observation of subsurface trickle irrigation, it was found that Dm/Hm ratio was within 1.52 to 1.91 and showed a decreasing tendency with respect to increasing rates of irrigation. 9. The location of sensors for subsurface trickle irrigation follows same pattern as above, with vertical distance from emitter being 10~17cm long and horizontal 22~25cm long. The location of emitter should be 50 cm. 10.The relationship between VS which is the volume of wetted soil and Q which is the total amount of water when soil is reached field capacity could be explained as VS= 2.914Q0.91and the irrigation rates showed no impacts on the above relationship.

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소아 잠복 결핵 감염 진단에 있어서 투베르쿨린 피부반응 검사와 결핵 특이항원 자극 Interferon-γ 분비능 측정의 비교 (Comparison of a whole blood Interferon-γ assay and A tuberculin skin test for detecting latent tuberculosis infection in children)

  • 전진경;김창기;김현숙;정귀영;인요한;김기환;이택진;전지현;김동수
    • Clinical and Experimental Pediatrics
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    • 제51권9호
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    • pp.971-976
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    • 2008
  • 목 적 : 결핵 감염 관리에 있어서 잠복 결핵 감염을 찾아 내어 예방적 화학요법을 시행하는 것은 주요 핵심이다. 결핵에 노출된 소아에서 잠복 결핵 감염 진단을 위해 투베르쿨린 피부반응과 최근 널리 쓰이기 시작한 체외 검사법으로 결핵 특이항원 자극에 의한 T 세포의 $IFN-{\gamma}$ 분비능 측정 (QFT-G IT)이 있다. 잠복 결핵 감염 진단에 있어 두 검사법의 차이를 알아보고자 본 연구를 진행하였다. 방 법 : 2006년 10월 1일부터 2007년 4월 30일까지 6개월 간 총 111명의 소아를 대상으로 투베르쿨린 반응과 QFT-G IT 검사를 시행하였고, 동일 기간 내인 2개월 간 진단 된 성인 결핵 환자 29명에서 동일한 검사 결과를 후향적으로 알아보았다. 결 과 : 결핵 환자와의 가까운 접촉력을 주소로 내원한 무증상의 환자 33명 중 15% (5명)에서 QFT-G IT 검사 양성 소견을 보였고, 투베르쿨린 피부반응은 42% (14명)에서 양성결과를 보여 두 검사 간의 일치율은 낮았다(${\kappa}=0.39$). 성인 결핵 환자에서 QFT-G IT 양성율은 86.2%였고, 결핵균 배양 검사 양성율은 48.2%였다. 배양검사 양성이면서 QFT-G IT 음성이었던 환자는 12.5%였다. 결 론 : 소아에서 잠복 결핵 감염을 진단함에 있어 가족력을 근저에 둔 투베르쿨린 피부반응 검사를 시행하되 접촉력이 불분명하거나 지속적이지 않았을 경우 혹은 BCG 접종 효과로 의심되는 경우 결핵균 특이항원 자극 $IFN-{\gamma}$ 분비능 측정이 보조적 수단으로 사용 될 수 있을 지는 아직 확실하지 않으며 향후 수년 간 다 기관 연구가 필요하다.

The Relationship between Temperature Patterns and Urban Morfometri in the Jakarta City, Indonesia

  • Maru, Rosmini;Ahmad, Shaharuddin
    • Asian Journal of Atmospheric Environment
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    • 제9권2호
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    • pp.128-136
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    • 2015
  • Sky View Factor (SVF) is one of the urban morfometri parameters that impact on the Urban Heat Island (UHI). SVF analisys was conducted in the city of Jakarta to investigate the relationship between urban temperature with urban morfometri. Jakarta City is the most populous city in the world that has a surrounding area $66,152km^2$ and the total population around 23 million people. The population of the city is the sixth highest in the world today. SVF measurements done by taking pictures at the six stations that have different morphological characteristics namely (1) the narrow streets Apartment Cempaka Mas (JS ITC), (2) the width of the road Apartment Cempaka Mas (JL ITC), (3) in front of Colleges Kanisius (DKK), (4) in front of office Journalist of Indonesia (DKWI), (5) Utan Kayu (UK), and (6) Tambun (TB). SVF value is obtained from the photgraphic image. Taking pictures at the location using a Nikon D90 camera with a Nikon Fisheye Nikkor 10.5 mm 1 : 2.8 G ED, further processed through a global mapper program. Therefore, the SVF derived from the six stations that vary 0.21 to 0.78. Temperature measurement is done during daylight hours from 06:00 am to 18:00 pm during the Western Part of Indonesia (WIB). Measurements performed at three different times, namely working days (HK) regular holidays (HCB) national holidays (HCN). The results showed that the highest average temperature of $33.32^{\circ}C$, occurring at UK station (SVF=0.45) at the time of HCB. Meanwhile, the average low temperature of $31.22^{\circ}C$ occurred at JLITC station (SVF=0.42). The two-time occurred on ordinary holidays. Maximum temperature of $38.4^{\circ}C$ occurred in Utan Kayu station (SFV=0.45) that occurred at 11.00 hrs, normal holidays. Furthermore minimum temperature 24.5 occurred at Tambun station (SVF=0.78) at 06.00 hrs in the morning at the usual holidays and national holidays. In general, the results showed that areas with large SVF has a lower temperature compared with areas with smaller SVF. Though, are not the only factors that matter, but this research may show that an increase in temperature in the city of Jakarta. Therefore, it is necessary to mitigate the serious from the government or society.

한국주요빙계의 소유역에 대한 순간단위권 유도에 관한 연구 (I) (Studies on the Derivation of the Instantaneous Unit Hydrograph for Small Watersheds of Main River Systems in Korea)

  • 이순혁
    • 한국농공학회지
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    • 제19권1호
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    • pp.4296-4311
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    • 1977
  • This study was conducted to derive an Instantaneous Unit Hydrograph for the accurate and reliable unitgraph which can be used to the estimation and control of flood for the development of agricultural water resources and rational design of hydraulic structures. Eight small watersheds were selected as studying basins from Han, Geum, Nakdong, Yeongsan and Inchon River systems which may be considered as a main river systems in Korea. The area of small watersheds are within the range of 85 to 470$\textrm{km}^2$. It is to derive an accurate Instantaneous Unit Hydrograph under the condition of having a short duration of heavy rain and uniform rainfall intensity with the basic and reliable data of rainfall records, pluviographs, records of river stages and of the main river systems mentioned above. Investigation was carried out for the relations between measurable unitgraph and watershed characteristics such as watershed area, A, river length L, and centroid distance of the watershed area, Lca. Especially, this study laid emphasis on the derivation and application of Instantaneous Unit Hydrograph (IUH) by applying Nash's conceptual model and by using an electronic computer. I U H by Nash's conceptual model and I U H by flood routing which can be applied to the ungaged small watersheds were derived and compared with each other to the observed unitgraph. 1 U H for each small watersheds can be solved by using an electronic computer. The results summarized for these studies are as follows; 1. Distribution of uniform rainfall intensity appears in the analysis for the temporal rainfall pattern of selected heavy rainfall event. 2. Mean value of recession constants, Kl, is 0.931 in all watersheds observed. 3. Time to peak discharge, Tp, occurs at the position of 0.02 Tb, base length of hlrdrograph with an indication of lower value than that in larger watersheds. 4. Peak discharge, Qp, in relation to the watershed area, A, and effective rainfall, R, is found to be {{{{ { Q}_{ p} = { 0.895} over { { A}^{0.145 } } }}}} AR having high significance of correlation coefficient, 0.927, between peak discharge, Qp, and effective rainfall, R. Design chart for the peak discharge (refer to Fig. 15) with watershed area and effective rainfall was established by the author. 5. The mean slopes of main streams within the range of 1.46 meters per kilometer to 13.6 meter per kilometer. These indicate higher slopes in the small watersheds than those in larger watersheds. Lengths of main streams are within the range of 9.4 kilometer to 41.75 kilometer, which can be regarded as a short distance. It is remarkable thing that the time of flood concentration was more rapid in the small watersheds than that in the other larger watersheds. 6. Length of main stream, L, in relation to the watershed area, A, is found to be L=2.044A0.48 having a high significance of correlation coefficient, 0.968. 7. Watershed lag, Lg, in hrs in relation to the watershed area, A, and length of main stream, L, was derived as Lg=3.228 A0.904 L-1.293 with a high significance. On the other hand, It was found that watershed lag, Lg, could also be expressed as {{{{Lg=0.247 { ( { LLca} over { SQRT { S} } )}^{ 0.604} }}}} in connection with the product of main stream length and the centroid length of the basin of the watershed area, LLca which could be expressed as a measure of the shape and the size of the watershed with the slopes except watershed area, A. But the latter showed a lower correlation than that of the former in the significance test. Therefore, it can be concluded that watershed lag, Lg, is more closely related with the such watersheds characteristics as watershed area and length of main stream in the small watersheds. Empirical formula for the peak discharge per unit area, qp, ㎥/sec/$\textrm{km}^2$, was derived as qp=10-0.389-0.0424Lg with a high significance, r=0.91. This indicates that the peak discharge per unit area of the unitgraph is in inverse proportion to the watershed lag time. 8. The base length of the unitgraph, Tb, in connection with the watershed lag, Lg, was extra.essed as {{{{ { T}_{ b} =1.14+0.564( { Lg} over {24 } )}}}} which has defined with a high significance. 9. For the derivation of IUH by applying linear conceptual model, the storage constant, K, with the length of main stream, L, and slopes, S, was adopted as {{{{K=0.1197( {L } over { SQRT {S } } )}}}} with a highly significant correlation coefficient, 0.90. Gamma function argument, N, derived with such watershed characteristics as watershed area, A, river length, L, centroid distance of the basin of the watershed area, Lca, and slopes, S, was found to be N=49.2 A1.481L-2.202 Lca-1.297 S-0.112 with a high significance having the F value, 4.83, through analysis of variance. 10. According to the linear conceptual model, Formular established in relation to the time distribution, Peak discharge and time to peak discharge for instantaneous Unit Hydrograph when unit effective rainfall of unitgraph and dimension of watershed area are applied as 10mm, and $\textrm{km}^2$ respectively are as follows; Time distribution of IUH {{{{u(0, t)= { 2.78A} over {K GAMMA (N) } { e}^{-t/k } { (t.K)}^{N-1 } }}}} (㎥/sec) Peak discharge of IUH {{{{ {u(0, t) }_{max } = { 2.78A} over {K GAMMA (N) } { e}^{-(N-1) } { (N-1)}^{N-1 } }}}} (㎥/sec) Time to peak discharge of IUH tp=(N-1)K (hrs) 11. Through mathematical analysis in the recession curve of Hydrograph, It was confirmed that empirical formula of Gamma function argument, N, had connection with recession constant, Kl, peak discharge, QP, and time to peak discharge, tp, as {{{{{ K'} over { { t}_{ p} } = { 1} over {N-1 } - { ln { t} over { { t}_{p } } } over {ln { Q} over { { Q}_{p } } } }}}} where {{{{K'= { 1} over { { lnK}_{1 } } }}}} 12. Linking the two, empirical formulars for storage constant, K, and Gamma function argument, N, into closer relations with each other, derivation of unit hydrograph for the ungaged small watersheds can be established by having formulars for the time distribution and peak discharge of IUH as follows. Time distribution of IUH u(0, t)=23.2 A L-1S1/2 F(N, K, t) (㎥/sec) where {{{{F(N, K, t)= { { e}^{-t/k } { (t/K)}^{N-1 } } over { GAMMA (N) } }}}} Peak discharge of IUH) u(0, t)max=23.2 A L-1S1/2 F(N) (㎥/sec) where {{{{F(N)= { { e}^{-(N-1) } { (N-1)}^{N-1 } } over { GAMMA (N) } }}}} 13. The base length of the Time-Area Diagram for the IUH was given by {{{{C=0.778 { ( { LLca} over { SQRT { S} } )}^{0.423 } }}}} with correlation coefficient, 0.85, which has an indication of the relations to the length of main stream, L, centroid distance of the basin of the watershed area, Lca, and slopes, S. 14. Relative errors in the peak discharge of the IUH by using linear conceptual model and IUH by routing showed to be 2.5 and 16.9 percent respectively to the peak of observed unitgraph. Therefore, it confirmed that the accuracy of IUH using linear conceptual model was approaching more closely to the observed unitgraph than that of the flood routing in the small watersheds.

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비열 유전체장벽방전 플라즈마의 포도상구균 및 대장균 살균효과 (Bactericidal Efficacy of Non-thermal DBD Plasma on Staphylococcus aureus and Escherichia coli)

  • 김기영;백남원;김용희;유관호
    • 한국산업보건학회지
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    • 제28권1호
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    • pp.61-79
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    • 2018
  • Objectives: The objective of this study was to examine the effect of non-thermal dielectric barrier discharge(DBD) plasma on decontamination of Staphylococcus aureus(S. aureus) and Escherichia coli(E. coli) as common pathogens. Methods: This experiment was carried out in a chamber($0.64m^3$)designed by the authors. The plasma was continuously generated by a non-thermal DBD plasma generator(Model TB-300, Shinyoung Air tech, Korea). Suspensions of S. aureus and E. coli of 0.5 McFarland standard($1.5{\times}10^8CFU/mL$) were prepared using a Densi-Check photometer(bio $M{\acute{e}}rieux$, France). The suspensions were diluted1:1000 in sterile PBS solutions(approximately$10^{4-5}CFU/mL$) and inoculated on tryptic soy agar(TSA) in Petri dishes. The Petri dishes(80mm internal diameter)were exposed to the non -thermal DBD plasma in the chamber. Results: The results showed that 95% of S. aureus colonies were killed after a six-hour exposure to the DBD plasma. In the case of E. coli, it took two hours to kill 100% of the colonies. The gram-negative E. coli had a greater reduction than the gram-positive S. aureus. This difference may be due to the structure of their cell membranes. The thickness of gram-positive bacteria is greater than that of gram-negative bacteria. The S. aureus is more resistant to DBD plasma exposures than is E. coli. It should be noted that average concentrations of ozone, a byproduct of the DBD plasma generator, were monitored throughout the experiment and the results were well below the criteria, 50 ppb, recommended by the Korean Ministry of the Environment. Thus, non-thermal DBD plasma is deemed safe for use in hospital and public facilities. Conclusions: There was evidence that non-thermal DBD plasma can effectively kill S. aureus and E. coli. The results indicate that DBD plasma technology can greatly contribute to the control of infections in hospitals and other public and private facilities.

항결핵제 감수성 결핵에서의 내성 변화 추이 (The Patterns of Acquiring Anti-Mycobacterial Drug Resistance by Susceptible Strains of Mycobacterium tuberculosis)

  • 이규택;정무상
    • 대한임상검사과학회지
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    • 제53권2호
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    • pp.137-142
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    • 2021
  • 본 연구는 결핵환자의 초기 치료에서 모든 항결핵제에 감수성을 보이는 경우, 치료 과정 중 항결핵제에 대한 내성 전환을 조사하였다. 2010년 1월부터 2019년 12월까지 111개 의료기관에서 녹십자의료재단에 항결핵제감수성 검사를 의뢰한 760건의 환자를 대상으로 하였다. 항결핵제에 모두 감수성인 594명중 추적 기간에 감수성에서 내성으로 전환되는 56명을 분석한 결과 INH, RIF, SM, QUI 순으로 단독 내성 전환율이 가장 높게 나타났으며, INH, RIF에 동시에 복합 내성을 보인 경우는 56명중 17명(30.4%)으로 높은 내성 전환율을 보이고 있다. 전환 시기는 INH 항결핵제는 최소 98일부터 1,862일, 평균 435.6일이며, RIF 항결핵제는 최소 108일부터 1,673일, 평균 457.7일로 분석되었다. 이에 본 연구는 결핵 초기 치료 후 모든 항결핵제에 감수성을 보였다면 3개월이 지난 시점에서 반드시 항결핵제 감수성 검사를 통해 내성 전환 및 다제내성결핵을 확인해야만 한다고 사료되며, 국민보건향상과 국민건겅증진을 위한 국가결핵관리 사업에 도움이 되었으면 한다.

Production of Vanillin from Ferulic Acid Using Recombinant Strains of Escherichia coli

  • Yoon Sang-Hwal;Li Cui;Lee Young-Mi;Lee Sook-Hee;Kim Sung-Hee;Choi Myung-Suk;Seo Weon-Taek;Yang Jae-Kyung;Kim Jae-Yeon;Kim Seon-Won
    • Biotechnology and Bioprocess Engineering:BBE
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    • 제10권4호
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    • pp.378-384
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    • 2005
  • Vanillin is one of the world's principal flavoring compounds, and is used extensively in the food industry. The potential vanillin production of the bacteria was compared to select and clone genes which were appropriate for highly productive vanillin production by E. coli. The fcs (feruloyl-CoA synthetase) and ech (enoyl-CoA hydratase/aldolase) genes cloned from Amycolatopsis sp. strain HR104 and Delftia acidovorans were introduced to pBAD24 vector with $P_{BAD}$ promoter and were named pDAHEF and pDDAEF, respectively. We observed 160 mg/L vanillin production with E. coli harboring pDAHEF, whereas 10 mg/L of vanillin was observed with pDAHEF. Vanillin production was optimized with E. coli harboring pDAHEF. Induction of the fcs and ech genes from pDAHEF was optimized with the addition of 13.3 mM arabinose at 18 h of culture, from which 450 mg/L of vanillin was produced. The feeding time and concentration of ferulic acid were also optimized by the supplementation of $0.2\%$ ferulic acid at 18 h of culture, from which 500 mg/L of vanillin was obtained. Under the above optimized condition of arabinose induction and ferulic acid supplementation, vanillin production was carried out with four different types of media, M9, LB, 2YT, and TB. The highest vanillin production, 580 mg/L, was obtained with LB medium, a 3.6 fold increase in comparison to the 160 mg/L obtained before the optimization of vanillin production.