• KSII Transactions on Internet and Information Systems (TIIS)
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• v.17 no.9
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• pp.2550-2572
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• 2023

Epidemiological survey is an important means for the prevention and control of infectious diseases. Due to the particularity of the epidemic survey, 1) epidemiological survey in epidemic prevention and control has a wide range of people involved, a large number of data collected, strong requirements for information disclosure and high timeliness of data processing; 2) the epidemiological survey data need to be disclosed at different institutions and the use of data has different permission requirements. As a result, it easily causes personal privacy disclosure. Therefore, traditional access control technologies are unsuitable for the privacy protection of epidemiological survey data. In view of these situations, we propose a black box-assisted fine-grained hierarchical access control scheme for epidemiological survey data. Firstly, a black box-assisted multi-attribute authority management mechanism without a trusted center is established to avoid authority deception. Meanwhile, the establishment of a master key-free system not only reduces the storage load but also prevents the risk of master key disclosure. Secondly, a sensitivity classification method is proposed according to the confidentiality degree of the institution to which the data belong and the importance of the data properties to set fine-grained access permission. Thirdly, a hierarchical authorization algorithm combined with data sensitivity and hierarchical attribute-based encryption (ABE) technology is proposed to achieve hierarchical access control of epidemiological survey data. Efficiency analysis and experiments show that the scheme meets the security requirements of privacy protection and key management in epidemiological survey.

• Asian-Australasian Journal of Animal Sciences
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• v.33 no.5
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• pp.836-847
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• 2020

Objective: We investigated the temporal expression profiles of long noncoding RNA (lncRNA) and mRNA in the peripheral blood of pigs during development and identified the lncRNAs that are related to the blood-based immune system. Methods: Peripheral blood samples were obtained from the pigs at 0, 7, 28, and 180 days and 2 years of age. RNA sequencing was performed to survey the lncRNA and mRNA transcriptomes in the samples. Short time-series expression miner (STEM) was used to show temporal expression patterns in the mRNAs and lncRNAs. Gene ontology and Kyoto encyclopedia of genes and genomes analyses were performed to assess the genes' biological relevance. To predict the functions of the identified lncRNAs, we extracted mRNAs that were nearby loci and highly correlated with the lncRNAs. Results: In total of 5,946 lncRNA and 12,354 mRNA transcripts were identified among the samples. STEM showed that most lncRNAs and mRNAs had similar temporal expression patterns during development, indicating the expressional correlation and functional relatedness between them. The five stages were divided into two classes: the suckling period and the late developmental stage. Most genes were expressed at low level during the suckling period, but at higher level during the late stages. Expression of several T-cell-related genes increased continuously during the suckling period, indicating that these genes are crucial for establishing the adaptive immune system in piglets at this stage. Notably, lncRNA TCONS-00086451 may promote blood-based immune system development by upregulating nuclear factor of activated T-cells cytoplasmic 2 expression. Conclusion: This study provides a catalog of porcine peripheral blood-related lncRNAs and mRNAs and reveals the characteristics and temporal expression profiles of these lncRNAs and mRNAs during peripheral blood development from the newborn to adult stages in pigs.

• Journal of Periodontal and Implant Science
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• v.46 no.5
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• pp.320-328
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• 2016

Purpose: Human saliva, as a vital part of the immune defense system, contains a number of distinct proteins and peptides. Recently human common salivary protein 1 (CSP1) has been identified as an abundant salivary protein and may play a role in promoting the binding of cariogenic bacteria to salivary pellicles. However, nothing else is known regarding the role of CSP1 in periodontology. The aim of this study was to quantify and compare CSP1 levels between healthy subjects and periodontal patients. Methods: This controlled clinical study was conducted in periodontally healthy individuals and patients with chronic periodontitis Chonbuk National University Hospital, with Institutional Review Board approval. Whole saliva samples were collected from 36 healthy subjects and 33 chronic periodontitis patients and analyzed. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immune blotting were conducted to ensure that anti-CSP1 monoclonal antibody (mAb) binds to CSP1 in human saliva. A sandwich enzyme-linked immunosorbent assay (ELISA) system was house-fabricated using mAb-hCSP1#14 and mAb-hCSP1#4 as a capture and a detector mAb, respectively. The CSP1 concentrations in saliva from 36 healthy subjects and 33 periodontal patients were quantified using the CSP1 sandwich ELISA system, and the results were analyzed using the Student's t-test. Results: Immunoblot analysis using mAb-hCSP1 as a probe confirmed that CSP1 in human saliva existed as a single band with a molecular weight of approximately 27-kDa. The quantification of CSP1 concentrations by CSP1 ELISA showed that the median values (25th to 75th percentiles) of periodontal patients and healthy subjects were 9,474 ng/mL (range, 8,434.10,139 ng/mL) and 8,598 ng/mL (range, 7,421.9,877 ng/mL), respectively. The Student's t-test indicated the presence of a statistically significant difference between the 2 groups (P=0.024). Conclusions: The presence of a significant difference in CSP1 levels between healthy subjects and periodontal patients suggests that CSP1 may be a potential biomarker for the detection or screening of periodontitis patients.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.38 no.12
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• pp.1427-1430
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• 2014

A thermal barrier coating (TBC) prevents heat directly transferring from a high-temperature flame to a substrate. The TBC system comprises a top coating and bond coating. TBC technology reduces the substrate surface temperature by about $100{\sim}170^{\circ}C$. In the TBC system, internal stress is generated by the difference in thermal expansion coefficients of the substrate and coating. The internal stress also differs according to the shape and position of the blade. In this study, finite element analysis was performed for different curvatures of coin-shaped specimens, which are commonly used for thermal fatigue tests, and the changes in internal stress of the TBC system were compared. Based on the results, the curvature at which the minimum stress occurs was derived, and the thermal stress was confirmed to increase with the difference between a given curvature and the curvature with the minimum stress.

• Journal of Microbiology and Biotechnology
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• v.27 no.7
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• pp.1276-1280
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• 2017

The mcr-1 gene is a new "superbug" gene discoverd in China in 2016 that makes bacteria highly resistant to the last-resort class of antibiotics. The mcr-1 gene raised serious concern about its possible global dissemination and spread. Here, we report a potential anti-resistant strategy using the CRISPR/Cas9-mediated approach that can efficiently induce mcr-1 gene knockout in Escherichia coli. Our findings suggested that using the CRISPR/Cas9 system to knock out the resistance gene mcr-1 might be a potential anti-resistant strategy. Bovine myeloid antimicrobial peptide-27 could help deliver plasmid pCas::mcr targeting specific DNA sequences of the mcr-1 gene into microbial populations.

• (The)Study of the Eastern Classic
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• no.54
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• pp.45-76
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• 2014

The purpose of this paper is to examine Toegye(退溪 李滉, 1501~1570)'s interpretation of Chungyong(中庸) who led the completion of the $Chos{\breve{o}}n$-style acceptance of Neo-Confucianism. This paper is focused on revealing the way that how he understood it according to the system of Neo-Confucianism that was proposed by Chu Hsi, rather than revealing the unique perspective of Toegye. I have the following configuration in this paper. First, I have set two directions of research for understanding of Chungyong, these were derived through the work that is an overview of cases of interpretation of Chungyong of $Chos{\breve{o}}n$. 1) How to understand the overall structure of Chungyong? 2) How to understand key concepts of Chungyong? Next, basing on these directions of research, I analyzed Toegye's interpretation of Chungyong. To grasp the structure of the whole, Toegye followed the segmentation system and structure of Chungyong changgu: Commentary on the Doctrine of the Mean, and to understand key concepts of Chungyong, he conducted in collaboration with concepts of Neo-Confucianism. Concretely, I analyze his work : Chungyong $suk{\breve{u}}i$(中庸釋義) and Chungyong $jil{\breve{u}}i$(中庸質疑) for asserting that he accepted the segmentation system and structure of Chungyong changgu. And I analyze his documents: letters to and from his disciples. This analysis focus on concepts of Chungyong for asserting that his understanding is in the context of Chu Hsi and other Neo-Confucian scholars's commentary. Toegye tried to reduce the diversity of interpretation and present one meaning.

• Journal of Veterinary Science
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• v.21 no.3
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• pp.46.1-46.18
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• 2020

Background: High concentrations of particulate matter less than 2.5 ㎛ in diameter (PM2.5) in poultry houses is an important cause of respiratory disease in animals and humans. Pseudomonas aeruginosa is an opportunistic pathogen that can induce severe respiratory disease in animals under stress or with abnormal immune functions. When excessively high concentrations of PM2.5 in poultry houses damage the respiratory system and impair host immunity, secondary infections with P. aeruginosa can occur and produce a more intense inflammatory response, resulting in more severe lung injury. Objectives: In this study, we focused on the synergistic induction of inflammatory injury in the respiratory system and the related molecular mechanisms induced by PM2.5 and P. aeruginosa in poultry houses. Methods: High-throughput 16S rDNA sequence analysis was used for characterizing the bacterial diversity and relative abundance of the PM2.5 samples, and the effects of PM2.5 and P. aeruginosa stimulation on inflammation were detected by in vitro and in vivo. Results: Sequencing results indicated that the PM2.5 in poultry houses contained a high abundance of potentially pathogenic genera, such as Pseudomonas (2.94%). The lung tissues of mice had more significant pathological damage when co-stimulated by PM2.5 and P. aeruginosa, and it can increase the expression levels of interleukin (IL)-6, IL-8, and tumor necrosis factor-α through nuclear factor (NF)-κB pathway in vivo and in vitro. Conclusions: The results confirmed that poultry house PM2.5 in combination with P. aeruginosa could aggravate the inflammatory response and cause more severe respiratory system injuries through a process closely related to the activation of the NF-κB pathway.

• (The)Study of the Eastern Classic
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• no.41
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• pp.7-35
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• 2010

Jang Hyungang(張顯光, 1554~1570) was one of the very famous Neo-Confucianists of T'oegye School in the Chosun dynasty. Jang, nevertheless, refused to accept Toegye's theory and had developed his own theory called li-qi-gyeong-wi-seol(理氣經緯說). So the theory was very unique in the history of Korean Neo-Confucianism. He matained that Tao(道), meaning the universal process which all beings including the human must follow, is accomplished by the unity of li(理, principle) and qi(ch'i, 氣, material force). He compared li and qi to warp(經) and woof(緯). According to his explanation, Tao is fulfilled by combining li and qi, as a textile is woven the combination of warps and woofs. He could, for I know, succeed to emphasize the unity of li and qii through this theory. Lots of Scholars in the Chosun dynasty criticized Jang's theory of li and qi for regarding li and qi as one, and thought that the theory originated from Luo Ch'inshun(羅欽順, 1465~1547), a famous Neo-Confucianist of in Early Ming China. Luo Ch'inshun maintained li-qi metaphysical monism that li and qi is united entity. In this respect, it seems that Jang's theory is similar to Luo's. However, I think that there are very important differences between the two. It is true that the main characteristics of Jang's theory is the unified relations between li and qi in this world. In the system of his philosophy, nevertheless, the topological position of li and qi are different in grade each other. Jang insisted that li is a supreme being to be materialized and realized through the movement of qi'in the process of universal development. Qi is accordingly only a kind of matter which completes li. In this sense, Jang's theory can be regarded as li metaphysical monism that li is the only substance. It means that Jang Hyeongwang's theory is a great contrast to Luo Ch'inshun's li-qi metaphysical monism.

• Journal of Distribution Science
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• v.6 no.1
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• pp.47-62
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• 2008

Joining the WTO in 2001, China became a number of the global economic system. China succeeded in vying to host Beijing 2008 Olympic Games and World Expo 2010 Shanghai. It is China's honor and opportunity to have high economic growth in the coming future. In 2007, the total cost of public logistics decreased by 18.2% than 2006 to 4540.6 billion RMB, accounting for 18.4% of the GDP. So, China logistics is a huge industry and a growing market full of charm. The statistic ratios of China's logistics and growth trends show us it is an important issue to build and run an effective logistics system. However, research on China's logistics systems and supply chain is lacked. This study is focus on the logistic location strategy in China including the study of factories and warehouses geographic strategy concerned with SCM. The core of this study is to propose a New Multi-Facilities Location Decision Model. This study banded the revised gravity center, the standard single facility location decision model(Gravity Center Model) and the transportation model into a new Multi-Facilities Location Decision Model. In addition, this study suggested the gravity center of population, the gravity center of each industry, the location decision graded-list of each industry of china using the gravity center model and the revised gravity center model. The new Multi-Facilities Location Decision Model proposed in this study can be used to solve the location decision problem of more than two facilities. And it can be used in the fields such as the location decision of production facility and service facility, the location of distribution and logistics, the location of broadcast and satellite communications, the location of wireless communication tower and so on.

• Journal of Animal Environmental Science
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• v.10 no.3
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• pp.169-174
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• 2004

The present study investigated the possibility of applying the fermented swine liquid manure handled with TAO system to hydroponic cultivation. The fermented swine liquid manure (FLM) used for the study was in low concentration of phosphoric acid (P). Hence, when doing hydroponic cultivation, the concentration of P and K was supplemented. The concentrations of N, P, and K in compensated FLMs were as fellows; (a) N 58mg/1, P 0.5mg/1 and K 31 mg/1 for FLM-1, (b) N 58mg/1, P 31 mg/l and K 39mg/1 for FLM-2, and (c) N 58mg/1, P 31 mg/1 and K 61 mg/1 for FLM-3, respectively. For hydroponic cultivation, it was possible to use diluted solution by 100 times through experiment of germination index. According to the experiment using lettuce, the compensated FLMs with P and K were superior in leaf width, yield and dry weight to FLM without any treatment. Therefore, it is necessary to supplement some nutrition before applying. In conclusion, it is highly estimated that the fermented swine liquid manure can be ap-plied to hydroponic cultivation. It is also necessary to conduct further researches related to nutritional compensation depending on the vegetables.