• The Journal of the Korea Contents Association
• /
• v.18 no.2
• /
• pp.269-279
• /
• 2018

The purpose of this research is to investigate how an elementary English class is presented in terms of communicative properties using COLT and TALOS because previous studies mainly used COLT. A lack of TALOS has shown on the previous studies. Also, this study takes a close look into whether the English class is communicative or not communicative since previous studies criticized in that elementary English classes are not communicative. For the purposes of this research, COLT part B and TALOS low-inference were used to analyze one elementary English class which had won the grand prize at English class contest. The result of this study revealed that the class is communicative in terms of high quantity and quality of students utterances, high ratio of students' discourse initiation, students' unpredictable information giving utterances and extension of utterances. Findings from this study revealed the good elementary English class characteristics of this class: students' participation, focus on affective atmosphere, students-directed activities, and unconscious internalization of target expressions through repetition.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
• /
• 2015.10a
• /
• pp.427-429
• /
• 2015

Standard missile, which is operated by our navy force by loading on DDH-II and DDG, is representative interceptors of U.S. Navy. To defend from rapidly increased air threats after World War II, U.S. Navy had accelerated the development of interceptors, and consequently 3Ts series(Tartar, Talos, Terrier) were developed. Since Standard Missile(SM) series were developed on the back of 3Ts, SM series has been positioned as main surface-to-air interceptors. In this paper, types and technical characteristics of Standard Missile Series from SM-1 to SM-6 are investigated and described.

• Journal of the Korean Magnetic Resonance Society
• /
• v.11 no.1
• /
• pp.30-41
• /
• 2007

The backbone molecular dynamics of the C-terminal part of the mouse Cdt1 protein (tCdt1, residues 420-557) was studied by high field NMR spectroscopy. The Secondary structure of this protein was suggested by analyzing of chemical shift of backbone atoms with programs TALOS and PECAN, together with NOE connectivities from 3D $^{15}N-HSQC-NOESY$ data. Measurement of dynamic parameters $T_1,\;T_2$ and NOE and limited proteolysis experiment provided information for domain organization of tCdt1(420-557). Analysis of the experimental data showed that the C-terminal part of the tCdt1 has well folded domain for residues 455-553. The residues 420-453 including ${\alpha}-helix$ (432-441) are flexible and probably belong to other functional domain in intact full length Cdt1 protein.

• Journal of Convergence for Information Technology
• /
• v.8 no.6
• /
• pp.231-236
• /
• 2018

Recently, a wide variety of IoT environment is being created due to the rapid development of information and communication technology. And accordingly in a variety of network structures, a countless number of attack techniques and new types of vulnerabilities are producing a social disturbance. In May of 2018, Talos Intelligence, the Cisco threat intelligence team has newly discovered 'VPN-Filter', which constitutes a large-scale IoT-based botnet, is infecting consumer routers in over 54 countries around the world. In this paper, types of IoT-based botnets and the attack techniques utilizing botnet will be examined and the countermeasure technique through EXIF metadata removal method which is the cause of connection method of C & C Server will be proposed by examining the characteristics of attack vulnerabilities and attack scenarios of VPN-Filter.

• Journal of the Korean Magnetic Resonance Society
• /
• v.20 no.2
• /
• pp.50-55
• /
• 2016

Backbone $^1H$, $^{13}C$ and $^{15}N$ resonance assignments are presented for the anticodon binding domain (residues 557-674) of human glycyl-tRNA synthetase (GRS). Role of the anticodon binding domain (ABD) of GRS as an anticancer ligand has recently been reported and its role in other diseases like Charcot-Marie-Tooth (CMT) and polymyositis have increased its interest. NMR assignments were completed using the isotope [$^{13}C/^{15}N$]-enriched protein and chemical shifts based secondary structure analysis with TALOS+ demonstrate similar secondary structure as reported in X-ray structure PDB 2ZT8, except some C-terminal residues. NMR signals from the N-terminal residues 557 to 571 and 590 to 614 showed very weak or no signals exhibiting dynamics or conformational exchange in NMR timescale.

• Proceedings of the Korean Society of Propulsion Engineers Conference
• /
• 2002.04a
• /
• pp.57-58
• /
• 2002

램제트는 다른 여러 추진시스템에 비하여 획기적으로 증가된 사거리를 가지므로 가용범위의 확대는 물론, 목표물 타격 시 높은 명중률과 낮은 격추율을 가지는 효과적인 추진 시스템이다. 램제트 엔진을 장착한 미사일로는 프랑스의 Griffon, ASMP, 미국의 Bomarc, Talos, 영국의 Bloodhound, Sea dart, 소련의 SA4, SA6 등을 비롯하여 많은 종류가 개발되어 실전 배치되었다. 근래 들어 램제트는 군사전략과 전술적인 목적 이외에도 민간용으로도 그 실용성이 강조되고 있어 그 중요성은 날로 더할 것으로 예측된다. 램제트는 일반적인 공기흡입식 엔진과는 달리 엔진 내부에 기계적으로 구동되는 부분이 없이 충격파를 통과하면서 공기의 압력이 높아지는 현상인 램압축 현상을 이용하여 공기를 압축하게 되므로 엔진의 구조가 간단하고, 상대적으로 높은 비추력과 추력/중량비를 가진다. 램제트는 정지 상태에서는 작동되지 않으며 사용 가능한 최소의 압력비를 줄 수 있는 비행 마하수에 도달해야 램제트가 작동하게 된다. 따라서 이러한 비행속도를 줄 수 있는 별도의 추진장치가 필요하게 되는데 이와 같은 보조 추진장치로 부스터를 사용한다. 부스터가 엔진의 내부에 장착된 램제트를 일제형 램제트 (IRR: integral Rocket Ramjet)라 부르며, 현대의 전략미사일과 민간용 초음속 항공기의 엔진에 도입되어 활발한 연구가 진행 중이다. 램압력을 이용하여 압축하므로 램제트의 설계시 설계점 비행 속도에서 전압력 손실이 최소가 되도록 설계되어야 하며, 이를 실험이나 수치해석을 통해 확인하여야 한다.

• Journal of the Korean Magnetic Resonance Society
• /
• v.17 no.1
• /
• pp.59-66
• /
• 2013

BldD, a developmental transcription factor from Streptomyces coelicolor, is a homodimeric, DNA-binding protein with 167 amino acids in each subunit. Each monomer consists of two structurally distinct domains, the N-terminal domain (BldD-NTD) responsible for DNA-binding and dimerization and the C-terminal domain (BldD-CTD). In contrast to the BldD-NTD, of which crystal structure has been solved, the BldD-CTD has been characterized neither in structure nor in function. Thus, in terms of structural genomics, structural study of the BldD-CTD has been conducted in solution, and in the present work, mainchain NMR assignments of the recombinant BldD-CTD (residues 80-167 of BldD) could be achieved by a series of heteronuclear multidimensional NMR experiments on a [$^{13}C/^{15}N$]-enriched protein sample. Finally, the secondary structure prediction by CSI and TALOS+ analysis using the assigned chemical shifts data identified a ${\beta}-{\alpha}-{\alpha}-{\beta}-{\alpha}-{\alpha}-{\alpha}$ topology of the domain. The results will provide the most fundamental data for more detailed approach to the atomic structure of the BldD-CTD, which would be essential for entire understanding of the molecular function of BldD.

• Journal of the Korean Magnetic Resonance Society
• /
• v.14 no.2
• /
• pp.105-116
• /
• 2010

SH3YL1, a novel protein containing one Src homology 3 domain at the carboxyl terminus was first detected in mouse anagen skin cDNA. This protein had a significant homology with YHRO 16c/Ysc 84, the yeast Src homology 3 domain-containing protein. The sequence identity was remarkable at the carboxyl and amino-terminal Src homology 3 domain, suggesting that the novel protein is a mouse homolog of the yeast protein and thus was termed as SH3YL1. SH3YL1 is composed of two domains, a DUF500 at N-termini and a SH3 domain at C-termini. In our study we cloned the SH3 domain in bacterial expression system in Escherichia coli using pET32a vector with TEV protease cleavage site and purified as a monomer using affinity chromatography. The N-terminal poly-Histidine tag was cleaved with TEV protease and target protein was used for backbone studies. Our study showed that SH3 domain primarily consists of $\beta$-sheet which is in consistence with previous result performed on the truncated SH3 domain of SH3YL1.

• Journal of the Korean Magnetic Resonance Society
• /
• v.16 no.2
• /
• pp.162-171
• /
• 2012

SAV2228 has an OB (Oligomer-Binding)-motif which is frequently used for nucleic acid recognition. To characterize the activity of translation initiation factor-1 (IF-1) from Staphylococcus aureus, SAV2228 was expressed and purified in Escherichia coli. We acquired 3D NMR spectra showing well dispersed and homogeneous signals which allow us to assign 94.4% of all $^1HN$, $^{15}N$, $^{13}C{\alpha}$, $^{13}C{\beta}$ and $^{13}CO$ resonances. We could predict a secondary structure of SAV2228 using TALOS and CSI from NMR data. SAV2228 was consisted of one ${\alpha}$-helix and five ${\beta}$-sheets. The predicted secondary structure, ${\beta}-{\beta}-{\beta}-{\alpha}-{\beta}-{\beta}$, was similar to other bacterial IF-1, but it was not completely same to the eukaryotic one. Assigned NMR peaks and secondary structre prediction can be used for the study on interaction with nucleic acid in the future.

• Journal of the Korean Magnetic Resonance Society
• /
• v.17 no.2
• /
• pp.105-110
• /
• 2013

HP1492 is a NifU-like protein of Helicobacter pylori (H. pylori) and plays a role as a scaffold which transfer Fe-S cluster to Fe-S proteins like Ferredoxin. To understand how to bind to iron ion or iron-sulfur cluster, HP1492 was expressed and purified in Escherichia coli (E. coli). From the NMR measurement, we could carry out the sequence specific backbone resonance assignment of HP1492. Approximately 91% of all resonances could be assigned unambiguously. By analyzing results of CSI and TALOS from NMR data, we could predict the secondary structure of HP1492, which consists of three ${\alpha}$-helices and three ${\beta}$-sheets. This study is an essential step towards the structural characterization of HP1492.