• Journal of Microbiology and Biotechnology
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• v.23 no.3
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• pp.322-328
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• 2013

In this study, we show the expression and purification of the human recombinant farnesoid X receptor (FXR)- ligand binding domain (LBD) protein in E. coli using a double cistronic vector, pACYCDuet-1, as a soluble form. We describe here the expression and characterization of a biologically active $FXR-LBD_{(248-476)}$. When expressed in the influence of bacterial promoters ($P_{T7}$ and $P_{Tac}$) of the single cistronic expression vectors, the human recombinant $FXR-LBD_{(248-476)}$ was found to be totally insoluble. However, by using a double cistronic expression vector, we were able to obtain the human recombinant $FXR-LBD_{(248-476)}$ in a soluble form. To allow for biological activities, we have subcloned into the pACYCDuet-1 vector, expressed in E. coli cells at some optimized conditions, and purified and characterized the human recombinant active $FXR-LBD_{(248-476)}$ proteins using the fluorescence polarization assay. This suggests that the expression of FXR-LBD in a double cistronic vector improves its solubility and probably assists its correct folding for the biologically active form of the proteins. We suggest that this may represent a new approach to high expression of other nuclear receptors and may be useful as well for other classes of heterodimeric protein partners.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.54 no.6
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• pp.1023-1029
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• 2021

The Japanese common squid Todarodes pacificus is one of the fish species within the total allowable catch (TAC) system which requires further investigation. In this study, the acoustic survey method was used to analyze the distribution of the Japanese common squid Todarodes pacificus across all the seas of South Korea. The sea area within Korea was investigated using the research vessels 20, 21, and 22 of the National Institute of Fisheries Science. The acoustic surveys were carried out from July to September 2019 and February to May 2020. The acoustic systems used in the survey had frequencies of 38 kHz and 120 kHz (EK60, EK80, Simrad, Norway) of the split-beam scientific echosounder. The results showed that, in spring, 277 m²/nmi² was the highest in the east sea area, and the same in the summer season 880 m²/nmi² was the highest in the east sea area. In autumn, the highest nautical area scattering coefficient (NASC) value was observed in the coastal portion of the south sea, and in winter, the NASC values were generally low in all the sea area.

• Safety and Health at Work
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• v.14 no.1
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• pp.118-123
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• 2023

Background: Chronic exposure to silica is related with the provocation of an inflammatory response and oxidative stress mechanism. Vitamin D has multiple benefits in biological activities particularly respiratory system disease. Method: In this research, 20 male Wistar rats were randomly allocated into four groups (5 rats /group) as follow: Group1 received saline as (negative control) group. The group 2 received a single IT instillation of silica (positive control) group; the group 3 was co-administrated with single IT silica and Vitamin D (20 mg/kg/day) daily for a period of 90 days. The rats of group 4 received Vitamin D daily for a period of 90 days. Results: Silica significantly increased serum and lung total Oxidant Status (TOS). Meanwhile, silica reduced serum and lung total antioxidant capacity (TAC), GSH and tumor necrosis factor-α (TNF-a). Vitamin D treatment meaningfully reversed oxidative stress, antioxidants status and inflammatory response. Also, Vitamin D improved histopathological changes caused by silica. Conclusion: These findings indicate that Vitamin D exerts protective effects against silica-induced lung injury. It seems that Vitamin D has potential use as a therapeutic object for silica induced lung injure.

• Journal of the Korean Society of Marine Environment & Safety
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• v.28 no.6
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• pp.890-898
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• 2022

Dispersion of suspended sediment, caused by coastal and marine development, is a key item in assessing marine environmental impact as it adversely affects marine life by increasing the level of turbidity and decreasing the amount of sunlight in seawater. However, its estimation has not been reliable because of the absence of a standard for the data measurement and divergent approaches to the impact assessment. In this study, we examined the estimation models from 58 Marine Environmental Impact Statements (MEISs, 2012-2014) to identify the gaps in the assessment and devise ways of improving the estimation. We developed four index items-grid system; unit load, particle size, and settling velocit-to evaluate their reliability in the estimation. The mean reliability score of each index was overall low-25 for grid system, 60 for unit load, 34 for particle size, and 17 for settling velocity. To ensure high reliability, it is important to develop a standard guideline that defines precise measurement of suspended sediment for unit load and settling velocity by particle size, followed by a grid system with compatible size for modelling. This can improve the estimation and thus underlie coherent impact assessment of suspended sediment dispersion on marine environment.

• Journal of the Korean Society of Marine Environment & Safety
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• v.27 no.5
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• pp.584-593
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• 2021

The sediment makes up the sea floor and plays an important role as a habitats for living things in the sea. However, pollution of coastal sediment due to internal and external factors such as inflow of contaminants from land and self-pollution becoming more serious, the needs for management of sediment that play an important role in ecology, is increasing. In particular, the review and evaluation of the effects of sediment in marine development projects including reclamation and dredging, have significance in terms of proactive protection and management of surrounding ecosystems. This study proposed the improvement measures for marine sediment management standards on the sea area utilization consultation for the development of public water. For the purpose, The evaluation and management standards of domestic and overseas marine sediments were reviewed and compared. The sediment environment guidelines under the Marine Environment Management Act of Korea were reviewed. Accordingly, the cases of Canada and NOAA, which have various evaluation standards consist of comprehensive factors, were analyzed. For analysis of operational cases, the port and fishing port development projects for the last five years (2016-2020) that are considered to have the greatest impact on sediments among the sea area use consultations were also reviewed. Finally, this study suggested that the assessment factors for ecological hazards and potential human risks should be considered in the sediment environmental standards in the sea area utilization consultation system.

• Journal of the Korean Society of Marine Environment & Safety
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• v.21 no.4
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• pp.361-371
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• 2015

This study analyzed the 91 cases of the statements for the Sea Area Utilization Consultation according to the developmental projects of ports and fishery harbors for 2012 2014 and the status of the record of document, and suggested the improvement way to go. The marine environmental timpact assessment items both marine chemistry such as water quality, sediment and marine biology such as benthic animal, plankton, and fisheries show highly rate of site survey. But, the utilization of those data through site survey is too low, and it is necessary to adopt the QA/QC for the reliability of survey data. The items of marine physics such as tide, tidal current analyzed based on references not a site survey. However, the simulation performed actively without calibration and verification compared to the result of site survey. When the projects of port and fishery harbor perform, it is necessary to monitor the physical parameter such as wave, tide and tidal current especially. Based on the scale and the type of project, we need introduce the system of scoping for prediagnosis the key assessment items and checklists.

• Journal of Radiation Protection and Research
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• v.18 no.1
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• pp.63-70
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• 1993

Double ionization of the K- shell accompanying K- shell electron capture of the 0.035 MeV transition of $^{125}I$ has been studied by counting coincidences between $K_{\alpha}$ hypersatellite X-rays and Ka satellite X-rays emitted when double vacancies are filled. The $^{125}I\;and\;^{125}Te^m$ source materials were used in the measurement. We obtained the coincidence spectrum using two NaI(T1) detectors and a Ge(Li) detector and TAC(Time-to-Amplitude Converter), and then analysed the measured coincidence number $N(K_{\alpha}^{II},\;K_{\alpha}^s)$, the total number $N(K_{\alpha})$ of K X-ray. The probability per K-shell electron capture that a double vacancy is formed, $P_{KK}$ is formed, $P_{KK}$ is found to be $2.15{\times}10^{-4}$.

• Journal of the Korean Society for Marine Environment & Energy
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• v.18 no.3
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• pp.179-188
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• 2015

This study reviewed the type of the project and developmental plan related to ocean energy development in the coastal land boundaries and the ocean, and suggested the efficient scoping method through the diagnosis of the key items of environmental impact assessment (EIA) in the coastal area. The major projects are the construction of tidal power plant, tidal current power plant, and offshore wind power plant in the public water, and also those are the construction of solar power plants in the coastal land boundaries. While the project plans on a large scale such as the construction of tidal power plant, it is important to consider both property of usefulness of the designated areas and harmony analysis with marine space availability based on the adequacy of the site selection and relevance of plan with the master plan for reclamation and strategic environmental assessment (SEA). And also it needs to be considered the careful checkup on the EIA checklist referring to the type of project, effective post-monitoring, and suggestion of mitigating methods to minimize the environmental impacts during the stage of actual environmental impact assessment. Introduction of a system of integrated marine environmental impact assessment should be considered for reasonable and effective manage to developmental projects on the marine spatial area.

• Journal of the Korean Society for Marine Environment & Energy
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• v.19 no.3
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• pp.227-235
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• 2016

This paper emphasized the necessity of the marine spatial planning (MSP) through the analysis of the major developmental projects which could make a contradiction based on the adequacy of the site selection and environmental impacts. The conflicting affairs between space utilization and management plan happen in the following ways: marine renewable energy development, sand mining, reclamation, construction of golf course in coastal area, thermal effluent and waste heat, erosion causing port development. The conflict of stakeholder continues caused by the accumulated environmental impact. For the reasons mentioned above, we found two things. First, it is necessary to comprehend the fact of developmental planning and MSP. Second, it is still unsatisfactory to connect the relevance of laws related to the spatial planning. For the reinforcement of marine environmental policy management, it is necessary to consolidate the property of site selection and assessment of developmental scale. Especially, while the strategic environmental assessment is in progress based on site selection and property of scale, consistent diagnosis is needed in the following concerns: the fact of the marine spatial planning, the relevance between national developmental plan and regional developmental plan, fisheries regulation, marine protected animals. For the environmentally sound and sustainable development (ESSD), MSP should have to be prepared based in a way of top-down including coastal and EEZ plan, relevance of ocean-use zoning and sector planning, 3-D spatial information. And also integrated information system have to be prepared through high-tech marine spatial information. In conclusion, consistent and relevant strategy for MSP should have to include the whole information related to the maritime affairs such as harbor, fishing port, fishing ground, coastal management, marine ecosystem generally.

• Journal of Fisheries and Marine Sciences Education
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• v.10 no.2
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• pp.139-161
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• 1998

This thesis includes a study on the legal regulation, the system and formalities on the control of illegal fishing. And the author analyzed the details of the lists of illegal fishing controlled by fishing patrol vessels of Ministry of Maritime Affairs and Fisheries from 1994 to 1996 in adjacent sea area of Korea. The results are summarized as follows ; 1. The fishing patrol vessels controlled total 826 cases in 2,726 days of 292 voyages by 17 vessels in 1994, total 1,086 cases in 3,060 days of 333 voyages by 18 vessels in 1995 and total 933 cases in 3,126 days of 330 voyages by 19 vessels in 1996. 2. The fishing period of illegal fishing was generally concentrated from April to September. But year after year, illegal fishing was scattered throughout the year. 3. The most controlled sea area of illegal fishing was the south central sea area in the sea near Port of Tongyeong. The sea area occupied about 36~51% of totality and the controlled cases were gradually increased every year. The second was the south western sea area in the sea near Port of Yosu. The sea area occupied about 18-27% and the controlled cases were a little bit increased every year. The third was the south eastern sea area in the sea near Pusan. The sea area occupied about 13~23% and the controlled cases were gradually decreased year by year. 4. The most controlled kind of illegal fishing was the small size bottom trawl. This occupied about 81-95% of totality and the controlled cases were gradually increased year by year. The second was the medium size bottom trawl. This occupied about 4-7% and the controlled cases were gradually decreased year by year. The third was the trawl of the coastal sea, this occupied about 2~4% and the controlled cases were a little bit decreased every year. 5. The most controlled address of illegal fishing manager was Pusan city which occupied about 33-51% of totality. The second was Cheonnam which occupied about 24-29%. The third was Kyungnam which occupied about 16~35%. 6. The most controlled violation of regulations was Article 57 of the Fisheries Act which occupied about 56-64% of totality. The second was Article 23 of Protectorate for Fisheries Resources which occupied about 21-36%. And the controlled cases by it were gradually increased every year.