• Korean Journal of Environmental Biology
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• v.26 no.2
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• pp.76-86
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• 2008

The seasonal variations of primary production were investigated by phyto-PAM chlorophyll fluorometry as well as classical methods (standing crops of phytoplanktons and chlorophyll ${\alpha}$) in the Beopsu Marsh, Haman-gun, Gyeongsangnam-do. The amounts of turbidity, SS, T-N, T-P, BOD, COD, Ca$^{2+}$ and Cl$^-$ were the highest at the station 3, where located in flowout site of wastewater treated by the filtration plants. The water quality was the third level by the standard of BOD and COD. The amount of chlorophyll a (268.8 mg L$^{-1}$) was the highest at the station 2 in April because the cell density (2,677 cells mL$^{-1}$) of Micractinium pusillum increased suddenly from February (180 cells mL$^{-1}$). The patterns of primary production of phytoplankton by phyto-PAM chlorophyll fluorometry were fallen in with those of standing crops and chlorophyll a of phyto-planktons. The primary production was varied according to stations and seasons. The water environments of the Beopsu Marsh as a natural mounument should be under the control of a regular examination in order to preserve the ecosystem.

• Journal of fish pathology
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• v.36 no.2
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• pp.263-275
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• 2023

Chum salmon (Oncorhynchus keta) is a species which returns to Korea for spawning and was produced as seed production at the Fisheries Resources Agency located in Uljin-gun, Gyeongsangbuk-do to preserve the species. However, farmed chum salmon showed symptoms of bacterial infection. Therefore, in this study, bacteria were isolated to identify the causative agent from chum salmon in October 2021. The isolated bacteria were identified based on the sequences of 16S rDNA, rpoD (RNA polymerase sigma factor σ⁷⁰), and vapA (A-layer) genes. Also, salinity-growth curve, biochemical characterization, antibiotic susceptibility test, and pathogenicity analysis were performed in four strains. As a result, four isolated strains were identified as Aeromonas salmonicida subsp. salmonicida. Additionally, the bacterial strains showed a decrease in growth as the salt concentration increased in the medium. All of the isolated strains exhibited γ-hemolysis, and the same biochemical properties. In the antimicrobial susceptibility test, all strains showed an inhibition zone of 40 to 44 mm for oxolinic acid, flumequine, and florfenicol. Pathogenic factors were assessed by RT-PCR at the mRNA level, and found that the four strains expresses the outer membrane ring of T3SS (ascV), inner membrane ring of T3SS (ascC), vapA, enterotoxin (act), and lipase (lip) genes which are well known to significantly contribute to the pathogenicity of A. salmonicida. The results of this study can be used as basic data to prevent A. salmonicida subsp. salmonicida occurring in sea-chum salmon in the future.

• Korean Journal of Soil Science and Fertilizer
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• v.45 no.2
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• pp.317-324
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• 2012

To reduce non-point source pollutants in Juam Lake eco-wetlands, purification efficiencies of pollutants were investigated at three different systems. The constructed wetlands (CWs) consisted of A system, B system and C system. A system consisted of $1^{st}$ free water surface (FWS) CW, $2^{nd}$ FWS CW, $3^{rd}$ FWS CW, $4^{th}$ subsurface flow (SSF) CW and $5^{th}$ SSF CW. B system consisted of $1^{st}$ FWS CW, $2^{nd}$ FWS CW, $3^{rd}$ FWS CW, $4^{th}$ FWS CW and $5^{th}$ SSF CW. C system consisted of $1^{st}$ FWS CW, $2^{nd}$ FWS CW, $3^{rd}$ FWS CW, $4^{th}$ FWS CW and $5^{th}$ SSF CW. The concentrations of BOD, COD, SS, T-N and T-P in inflow ranged 0.20 ~ 0.91, 1.24 ~ 8.00, 0.60 ~ 8.60, 0.04 ~ 2.50 and $0.001{\sim}0.685mg\;L^{-1}$ from March to October in 2011, respectively. Removal rates of BOD, SS, T-N and T-P were high in Autumn, Spring, Spring and Summer, respectively. In A system, $1^{st}$ FWS CW, $2^{nd}$ FWS CW and $3^{rd}$ FWS CW were dominated by Leersia oryzoides. In B system, $1^{st}$ FWS CW, $3^{rd}$ FWS CW and $4^{th}$ FWS CW were dominated by Leersia oryzoides. In C system, $2^{nd}$ FWS CW and $3^{rd}$ FWS CW were dominated by Nymphaea teragona.

• Journal of Korean Society on Water Environment
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• v.27 no.5
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• pp.719-728
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• 2011

The results of investigation on the water quality of the unit watersheds in the Yeongsang/Seomjin River indicate that $BOD_5$, SS, T-P decrease in most of the unit watersheds. However, $COD_{Mn}$ and T-N increase since the Total Maximum Daily Loads (TMDLs). It is thought that $COD_{Mn}$, which is included non-biodegradable matters, is difficult to decrease only using by conventional biological treatment facilities and T-N is affected by non-point source, etc. The results of assessment on 3 years $BOD_5$ indicate that most of the unit watersheds were being improved except Yeongbon A, Seombon C and Yocheon B. In addition, it was found that T-P were also being improved except Yocheon B and Hwangryeong A. Consequently, water qualities of the unit watersheds have been improved in many cases since the TMDLs.

• Journal of Wetlands Research
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• v.2 no.1
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• pp.87-94
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• 2000

The purpose of this study was to analysis the pollutant loading of Chin yang Reservoir according to the variation of outflow. Regression equation of the pollutant loading and outflow was represented as $L=a\;Q^b$ in which L = pollutant loading(kg/day), a and b = regression coefficient, and Q = outflow($m^3/day$). Regression coefficients ($R^2$) of Sanchung, Sinan and Changchon site was in range of 0.8376 to 0.9818. Therefore the pollutant loading was good correlated with outflow. Changchon site had minimum b value because outflow of pollutant was little compared with rainfall. The SS was the highest b value 1.621~1.7834 among water quality parameters because the pollutant loading of SS was much affected by outflow. Also, the pollutant loadings per area could be calculated and compared in case of the dry season, normal season and flood season. The pollutant loading in the normal and flood season except the dry season were higher in order of Sanchung, Sinan and Changchon site. Pollutant loading per area were higher in order of Sinan, Sanchung and Changchon site. When it compared with pollutant loading per area calculated using pollutant unit loading, T-N was much different each other.

• Asian Oncology Nursing
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• v.6 no.1
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• pp.15-26
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• 2006

Purpose: This study was to assess relationships among the uncertainty, medical staff's support, and anxiety perceived by family members with cancer patients while the family members were waiting for their patients undergoing surgery. Method: The data were collected from the family members of cancer patients who were undergoing surgery in D University Hospital at B city from February 1 to April 12, 2005. The used instruments were the State Anxiety Scale of Spielberger's(1975) STAI, Mishel's Uncertainty in Illness Scale (MUIS)(1981), and Relationship Questionnaires (Lee, 1978). The collected data was analyzed by using t-test, ANOVA, Pearson's coefficients, and stepwise multiple regression. Results: As the result, the most influential variable explaining anxiety of family members was uncertainty $({\ss}=0.37)$, followed by perceived illness state $({\ss}=-0.27)$. These two variables simultaneously explained 29.3% of the variance in anxiety. Conclusion: We suggest to develop a nursing intervention program to reduce the uncertainty through the medical staff's support and o test its effects.

• Korean Journal of Ecology and Environment
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• v.40 no.2
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• pp.264-272
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• 2007

Runoff of non-point pollutants has affected bad influence to water quality of river as reaching within short time. For this reason, reducing them prior to reaching aquatic systems or treating them after collection from discharge process of pollutants are desirable for efficient treatment of pollutants. This study was carried out to develop an ecotechnological method to prevent further aggravation of water quality by non-point source through vegetation filter strips. This study has placed a focus on improving infiltration capacity of soil for the optimum condition of vegetation filter strips. Therefore, we used titled soil filter strips instead of vegetation filter strips in this study. The three types of soil tilter strips were used in a bench scale experiment before applying to the field. The reduction efficiency of pollutants in soil filter strips (SS $84.5{\sim}92.5%$, BOD $67.9{\sim}80.6%$, T-N $43.4{\sim}76.6%$, T-P $40.6{\sim}87.4%$, Cu $28.3{\sim}48.1%$ Fe $92.1{\sim}97.7%$, Pb $81.4{\sim}97.3%$) was much higher than that of the controled group. And non-point pollutants reduction efficiency by soil filter strip's forms was estimated to be distinguishing in order of bio material, mixture of sand and gravel and lastly the whole gravel. In the event, the whole reduction efficiency of pollutants on the soil filter strips disclosed good results.

• Ecology and Resilient Infrastructure
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• v.3 no.4
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• pp.231-237
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• 2016

The purpose of this study is to analyze phosphate adsorption characteristic of the filter media applied in water purification technology. And it is also to observe the removal efficiency of the technology that was developed by combining the purification abilities of filter media and the vegetation mat. The filter media, Adphos, is the subject of this study. The result of adsorption test shows that $PO_4{^{3-}-P}$ adsorption characteristics of Adphos is determined by the Langmuir isotherm model fitting and the $q_m$ (maximum adsorption amount) value is calculated as 1.162 mg/g. To verify the removal efficiency of the purification technology, a lab-scale reactor, including a 400 mm depth of filter bed filled by Adphos, was manufactured. Yellow flag Iris (Iris pseudacours L.) was planted on the vegetation bed and the coir-roll was used to fix the root of vegetation. The reactor ran 8 hours per day over 71 days, and the results of filtration experiment show that average removal efficiency of SS, T-N and T-P were calculated as 94%, 41% and 64% respectively. With these results, it was proved that the purification technology is highly effective. And for the long-time use, a maintenance guide is also required.

• Asian-Australasian Journal of Animal Sciences
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• v.29 no.3
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• pp.413-418
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• 2016

Myostatin (MSTN) is a secreted growth factor expressed in skeletal muscle and adipose tissue that negatively regulates skeletal muscle mass. Gene knockout of MSTN can result in increasing muscle mass in sheep. The objectives were to investigate whether myostatin gene can be edited in sheep by transcription activator-like effector nucleases (TALENs) in tandem with single-stranded DNA oligonucleotides (ssODNs). We designed a pair of TALENs to target a highly conserved sequence in the coding region of the sheep MSTN gene. The activity of the TALENs was verified by using luciferase single-strand annealing reporter assay in HEK 293T cell line. Co-transfection of TALENs and ssODNs oligonucleotides induced precise gene editing of myostatin gene in sheep primary fibroblasts. MSTN gene-edited cells were successfully used as nuclear donors for generating cloned embryos. TALENs combined with ssDNA oligonucleotides provide a useful approach for precise gene modification in livestock animals.

• Journal of Environmental Science International
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• v.24 no.2
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• pp.221-228
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• 2015

In this research traditional Buddhist dietary life style was studied as an alternative for both saving water and decreasing water pollution from household. A traditional dietary life style for the Buddhist monks, called Balwoo Gongyang, and its modernized one for citizens, called Dish Gongyang were examined with water use amount, wastewater quality and pollutant loading rate, and were compared with the kitchen wastewater from household. And several types of natural detergent were also examined to compare their effect on the wastewater quality and pollutant loading rate. This research was carried out using the wastewater from Balwoo and Dish Gongyang in J building located in Seoul. Wastewater generation from Balwoo Gongyang that was about $1.0{\ell}$/capita/day was very low compare to that from dish washing in normal household($32.6{\ell}$/capita/day). In case of Dish Gongyang, water generation was 1.8 times higher than that from Balwoo Gongyang, but it was just 1/19.2 of normal household. When the wastewater quality of Balwoo Gongyang was compared with the kitchen wastewater of normal household, SS was 1/16($15mg/{\ell}$), COD was 1/7($22.1mg/{\ell}$), BOD was 1/9($24.1mg/{\ell}$) and T-N was 1/16($1.7mg/{\ell}$). Pollutant loading rate from Balwoo Gongyang was very low, COD 18.0 mg/capita/day, SS 12.3 mg/capita/day and it was 1/290, 1/639 of that from dish washing wastewater from normal household, respectively. Pollutant loading rate from Dish Gongyang was also low 1/13(SS)~1/144(TN) compared to dish washing wastewater from normal household. As a natural detergent, used water from washing rice showed very low pollutant loading rate. It was concluded that Dish Gongyang that was a modernized and simplified dietary life style of Balwoo Gongyang, was very eco-friendly. Especially when it was combined with natural detergents using by-product from cooking process, its effect on the water pollution was very low.