• Journal of Microbiology
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• v.42 no.2
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• pp.111-116
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• 2004

It is known that Bacillus subtilis glutamyl-tRNA synthetase (GluRS) mischarges E. coli $tRNA_{1}$$^{Gln}$ with glutamate in vitro. It has also been established that the expression of B. subtilis GluRS in Escherichia coli results in the death of the host cell. To ascertain whether E. coli growth inhibition caused by B. subtilis GluRS synthesis is a consequence of Glu-$tRNA_{1}$$^{Gln}$ formation, we constructed an in vivo test system, in which B. subtilis GluRS gene expression is controlled by IPTG. Such a system permits the investigation of factors affecting E. coli growth. Expression of E. coli glutaminyl-tRNA synthetase (GlnRS) also amelio-rated growth inhibition, presumably by competitively preventing $tRNA_{1}$$^{Gln}$ misacylation. However, when amounts of up to 10 mM L-glutamine, the cognate amino acid for acylation of $tRNA_{1}$$^{Gln}$, were added to the growth medium, cell growth was unaffected. Overexpression of the B. subtilis gatCAB gene encoding Glu-$tRNA^{Gln}$ amidotransferase (Glu-AdT) rescued cells from toxic effects caused by the formation of the mis-charging GluRS. This result indicates that B. subtilis Glu-AdT recognizes the mischarged E. coli Glu-$tRNA_{1}$$^{Gln}$, and converts it to the cognate Gln-$tRNA_{1}$$^{Gln}$ species. B. subtilis GluRS-dependent Glu-$tRNA_{1}$$^{Gln}$ formation may cause growth inhibition in the transformed E. coli strain, possibly due to abnormal protein synthesis.

• International Journal of Oral Biology
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• v.43 no.3
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• pp.113-121
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• 2018

Taste is closely related to intake of food. Taste perception is also influenced by type of food ingested, and nutrition and health status. Bitter taste plays an important role in the survival of human and animals to avoid probable toxic and harmful substances. Vertebrate animals recognize bitter taste through type 2 taste receptors (T2Rs). Several T2Rs have been expressed extra-oral such as the gastrointestinal tract, respiratory tract, urogenital tract, brain and immune cells, and parts of their functions are being revealed. This review will discuss physiological roles of T2Rs in relation to innate immunity, secretion and smooth muscle contraction expressed in extra-oral cells and tissues, and we summarize relationships between polymorphisms in T2Rs and general or oral diseases. It is not a coincidence that animals pay much genetic costs for taste and smell during evolution.

• Proceedings of the IEEK Conference
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• 2001.09a
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• pp.245-248
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• 2001

본 논문에서는 연집 오류(burst error)에 우수한 정정 능력을 보이는 고속 RS(Reed-Solomon) 복호기를 제안한다. 제안된 RS 복호기는 RS(n, k, t); (37 < n ≤ 255, 21 < k ≤ 239, t = 8)의 사양을 지원하며 수정 유클리드 알고리즘(modified Euclid´s algorithm)을 이용한 시스톨릭 어레이(systolic array) 방식의 병렬처리 구조로 설계되었다. 고속 RS 복호기의 효율적인 VSLI 설계를 위하여 새로운 방식의 수정 유클리드 알고리즘 연간 회로를 제안한다. 제안된 수정 유클리드 알고리즘 회로는 2t + 1의 연산 지연 시간을 갖으며 기존 구조의 연산 지연 시간인 3t + 37에 비하여 t = 8 인 경우 약 72%의 연산 지연이 감소하였다. 제안된 구조를 VHDL을 이용하여 설계하였으며 SAMSUNG 0.5㎛(KG80) 라이브러리를 이용하여 논리 합성과 타이밍 검증을 수행하였다. 합성된 RS 복호기의 총 게이트 수는 약 77,000 개이며 최대 80MHz의 동작 속도를 나타내었다.

• Asian Pacific Journal of Cancer Prevention
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• v.14 no.10
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• pp.5839-5842
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• 2013

Aim: We assessed the association between genetic variants of XPG, XPA, XPD, CSB, XPC and CCNH in the nucleotide excision repair (NER) pathway and risk of prostate cancer. Methods: We genotyped the XPG, XPA, XPD, CSB, XPC and CCNH polymorphisms by a 384-well plate format on the MassARRAY(R) platform. Multivariate logistical regression analysis was used to assess the associations between the six gene polymorphisms and risk of prostate cancer. Results: Individuals carrying the XPG rs229614 TT (OR=2.01, 95%CI=1.35-3.27) genotype and T allele (OR=1.73, 95%CI=1.37-2.57) were moderately significantly associated with a higher risk of prostate cancer. Subjects with XPD rs13181 G allele had a marginally increased risk of prostate cancer, with adjusted OR(95%CI) of 1.53 (1.04-2.37). Moreover, individuals carrying with CSB rs2228526 GG genotype (OR=2.05, 95% CI=1.23-3.52) and G allele (OR=1.56, 95%CI=1.17-2.05) were associated with a higher increased risk of prostate cancer. The combination genotype of XPG rs2296147 T and CSB rs2228526 G allele had accumulative effect on the risk of this cancer, with an OR (95% CI) of 2.23(1.37-3.59). Conclusions: Our study indicates that XPG rs2296147 and CSB rs2228526 polymorphisms are significantly associated with increased risk of prostate cancer, and that combination of XPG rs2296147 T allele and CSB rs2228526 G allele is strongly associated with an increased risk.

• Journal of the Institute of Electronics Engineers of Korea TC
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• v.41 no.3
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• pp.187-187
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• 2004

In this paper, we present an efficient architecture of a versatile Reed-Solomon (RS) decoder which can be programmed to decode RS codes continuously with my message length k as well as any block length n. This unique feature eliminates the need of inserting zeros for decoding shortened RS codes. Also, the values of the parameters n and k, hence the error-correcting capability t can be altered at every codeword block. The decoder permits 3-step pipelined processing based on the modified Euclid's algorithm (MEA). Since each step can be driven by a separate clock, the decoder can operate just as 2-step pipeline processing by employing the faster clock in step 2 and/or step 3. Also, the decoder can be used even in the case that the input clock is different from the output clock. Each step is designed to have a structure suitable for decoding RS codes with varying block length. A new architecture for the MEA is designed for variable values of the t. The operating length of the shift registers in the MEA block is shortened by one, and it can be varied according to the different values of the t. To maintain the throughput rate with less circuitry, the MEA block uses both the recursive technique and the over-clocking technique. The decoder can decodes codeword received not only in a burst mode, but also in a continuous mode. It can be used in a wide range of applications because of its versatility. The adaptive RS decoder over GF($2^8$) having the error-correcting capability of upto 10 has been designed in VHDL, and successfully synthesized in an FPGA chip.

• Journal of the Institute of Electronics Engineers of Korea TC
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• v.41 no.3
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• pp.29-38
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• 2004

In this paper, we present an efficient architecture of a versatile Reed-Solomon (RS) decoder which can be programmed to decode RS codes continuously with my message length k as well as any block length n. This unique feature eliminates the need of inserting zeros for decoding shortened RS codes. Also, the values of the parameters n and k, hence the error-correcting capability t can be altered at every codeword block. The decoder permits 3-step pipelined processing based on the modified Euclid's algorithm (MEA). Since each step can be driven by a separate clock, the decoder can operate just as 2-step pipeline processing by employing the faster clock in step 2 and/or step 3. Also, the decoder can be used even in the case that the input clock is different from the output clock. Each step is designed to have a structure suitable for decoding RS codes with varying block length. A new architecture for the MEA is designed for variable values of the t. The operating length of the shift registers in the MEA block is shortened by one, and it can be varied according to the different values of the t. To maintain the throughput rate with less circuitry, the MEA block uses both the recursive technique and the over-clocking technique. The decoder can decodes codeword received not only in a burst mode, but also in a continuous mode. It can be used in a wide range of applications because of its versatility. The adaptive RS decoder over GF(2$^{8}$ ) having the error-correcting capability of upto 10 has been designed in VHDL, and successfully synthesized in an FPGA chip.

• Journal of Mushroom
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• v.16 no.3
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• pp.225-230
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• 2018

This study was conducted to reuse spent mushroom substrates (SMS) of Pleurotus ostreatus and improve their nitrogen content by bacterial treatment. Two kinds of bacteria were used to investigate the increase in total nitrogen (T-N) content. Bacillus sp. (GM20-4) was isolated from SMS of oyster mushroom, and Rhodobacter sphaeroides (RS) was obtained from Gwangju Si Agricultural Technology Center. SMS samples were collected from three oyster mushroom cultivation farms located in Gyeonggi-do province, Korea. When dried SMS was inoculated with 30% culture broth of GM20-4 and RS and incubated at room temperature ($25{\pm}2^{\circ}C$) for 5 days, T-N content increased. To investigate the T-N content of other SMS, three dried SMS samples (A, B, and C) were treated by the same method using GM20-4 and RS. As a result, the T-N content of sample B was 20% higher than that of the control, whereas the T-N content of samples A and C increased to 17% and 12%, respectively. The change in T-N content by bacterial treatment of wet SMS was slightly higher than that of the control. The changes in amino acid content were also found to be higher than those in the control in all SMS samples by GM20-4 and RS treatment. Aspartic acid and glutamic acid contents were the highest among all amino acid compositions. Especially, the aspartic and glutamic acid contents of sample B increased by 2.9 folds higher than the control.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.10
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• pp.4319-4323
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• 2014

Background: Phospholipase C epsilon 1 (PLCE1) encodes a member of the phospholipase family of proteins that play crucial roles in carcinogenesis and progression of several cancers including esophageal cancer (EC). In two large scale genome-wide association studies (GWAS) single nucleotide polymorphisms (SNP, rs2274223A>G, rs3765524C>T) in PLCE1 were identified as novel susceptibility loci of esophageal cancer (EC) in China. The aim of the present study was to investigate this finding in Kashmir Valley, a high risk area. Materials and Methods: We determined genotypes of three potentially functional SNPs (rs2274223A>G, rs3765524C>T and rs7922612C>T) of PLCE1 in 135 EC patients, and 195 age and gender matched controls in Kashmiri valley by PCR RFLP method. Risk for developing EC was estimated by binary logistic regression using SPSS. Results: The selected PLCE1 polymorphisms did not show independent association with EC. However, the $G_{2274223}T_{3765524}T_{7922612}$ haplotype was significantly associated with increased risk of EC (OR=2.92; 95% CI=1.30-6.54; p=0.009). Smoking and salted tea proved to be independent risk factors for EC. Conclusions: Genetic variations in PLCE1 modulate risk of EC in the high risk Kashmiri population.

• Genomics & Informatics
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• v.18 no.3
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• pp.27.1-27.12
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• 2020

The prevalence of early-onset type 2 diabetes (EOT2D) is increasing in Asian countries. Genome-wide association studies performed in European and various other populations have identified associations of numerous variants with type 2 diabetes in adults. However, the genetic component of EOT2D which is still unexplored could have similarities with late-onset type 2 diabetes. Here in the present study we aim to identify the association of variants with EOT2D in South Indian population. Twenty-five variants from 18 gene loci were genotyped in 1,188 EOT2D and 1,183 normal glucose tolerant subjects using the MassARRAY technology. We confirm the association of the HHEX variant rs1111875 with EOT2D in this South Indian population and also the association of CDKN2A/2B (rs7020996) and TCF7L2 (rs4506565) with EOT2D. Logistic regression analyses of the TCF7L2 variant rs4506565(A/T), showed that the heterozygous and homozygous carriers for allele 'T' have odds ratios of 1.47 (95% confidence interval [CI], 1.17 to 1.83; p = 0.001) and 1.65 (95% CI, 1.18 to 2.28; p = 0.006) respectively, relative to AA homozygote. For the HHEX variant rs1111875 (T/C), heterozygous and homozygous carriers for allele 'C' have odds ratios of 1.13 (95% CI, 0.91 to 1.42; p = 0.27) and 1.58 (95% CI, 1.17 to 2.12; p = 0.003) respectively, relative to the TT homozygote. For CDKN2A/2B variant rs7020996, the heterozygous and homozygous carriers of allele 'C' were protective with odds ratios of 0.65 (95% CI, 0.51 to 0.83; p = 0.0004) and 0.62 (95% CI, 0.27 to 1.39; p = 0.24) respectively, relative to TT homozygote. This is the first study to report on the association of HHEX variant rs1111875 with EOT2D in this population.

• Molecules and Cells
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• v.22 no.2
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• pp.127-132
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• 2006

In the post genome project era, it is well established that the human genome contains a smaller number of genes than expected. The complexity found in higher organisms can be explained if proteins are multifunctional. Indeed, recent studies are continuing to reveal proteins that are capable of a broad repertoire of functions. A good paradigm for multifunctionality can be found in the amino-acyl tRNA synthetases (aaRSs), an ancient conserved family of proteins. This unique family, which is comprised of 20 different enzymes, is well known for its participation in protein synthesis. Several studies have described numerous examples of these "housekeeping" proteins taking part in extensive critical cellular activities. In this review, we focus on a member of that family, lysyl-tRNA synthetase (LysRS), which has been shown to have a dual functionality. In addition to its contribution to the translation process, LysRS also takes part in the regulation of MITF and USF2 target genes. This phenomenon was first described in mast cells.