• Title/Summary/Keyword: T1/E1

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Study of the Kinetics and Mechanisms of Alkoxy Radical Reactions in the Gas Phase (I). Arrhenius Parameters for t-Butoxy Radical Reactions with Isobutane and Cyclohexane

  • Song, Se-Ahn;Choo, Kwang-Yul
    • Bulletin of the Korean Chemical Society
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    • 제5권1호
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    • pp.16-21
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    • 1984
  • The relative Arrhenius parameters for t-butoxy radical decomposition (log $A_d$, $E_d$) and hydrogen abstraction of t-butoxy radical from hydrogen donor (log $A_d$, $E_d$) by competitive method were obtained as follows: for cyclohexane; log $A_a/A_d$ = -4.17 mole/l and $E_d-E_a$ = 9.01 kcal/mole, for isobutane; log $A_a/A_d$ = -5.70 mole/l and $E_e-E_a$= 11.0 kcal/mole. From the reported Arrhenius parameters for t-Butoxy radical decomposition reactions the parameters for t-Butoxy radical reactions with isobutane and cyclohexane are estimated to be log $A(l/mol{\cdot}sec)$ = 8.4, $E_a$ = 4.3 kcal/mol and $log A (l/mol{\cdot}sec)= 9.9,\;E_a$ = 6.3 kcal/mol, respectively.

Nd-Fe-B 자석의 정자기장이 MC3T3-E1 세포의 alkaline phosphatase 활성도에 미치는 영향 (The effect of a static magnetic field of Nd-Fe-B magnet on alkaline phosphatase activity of MC3T3-E1 cells)

  • 김숙희;권오원;류현모
    • 대한치과교정학회지
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    • 제30권2호
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    • pp.205-214
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    • 2000
  • Nd-Fe-B 자석의 정자기장 (Static Magnetic Field)이 MC3T3-E1 세포의 alkaline phosphase (ALP) 활성도에 미치는 영향을 알아보기 위하여, MC3T3-El세포를 12 well 세포배양접시의 1열과 3열에 접종하고 1열과 3열의 첫째 칸 하방에 Nd-Fe-B 자석을 가하여 7일, 13일, 19일, 25일간 배양한 후 세포의 ALP 활성도를 측정하였으며, 100 mm 세포배양접시의 한 쪽 가장자리 하방에 Nd-Fe-B 자석을 가하여 7일, 13일, 19일, 25일간 배양한 후 ALP 염색을 하여 다음과 같은 결과를 얻었다. 1. 정자기장을 가한 13, 19, 25일에 각각 100 mT에서는 대조군에 비해 ALP활성도가 감소된 반면 4.6 mT와 0.5 mT에서는 대조군에 비해 ALP 활성도가 증가되었다 (P<0.01). 2. ALP염색에서 전체적으로는 19일까지 ALP가 증가되었다가 25일에 다소 감소되는 양상을 나타내었으며, 각 세포배양접시에서는 7일에는 뚜렷한 차이를 보이지 않았으나 13, 19, 25일에는 자석이 놓인 부위 (100 mT) 가 그 반대편(0.5 mT)에 비해 ALP가 감소되었음을 육안으로나 도립위상차현미경으로 관찰할 수 있었다. 이상의 결과 0.5 mIT와 4.6 mT의 낮은 자기장에서는 ALP 활성도가 증가되었으며 100 mT의 높은 자기장에서는 ALP활성도가 감소되어, Nd-Fe-B자석의 정자기장이 MC3T3-E1 세포의 ALP 활성도에 영향을 미쳐 골조직의 형성 및 개조에 영향을 미칠 수 있을 것으로 생각된다.

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INTEGRAL BASES OVER p-ADIC FIELDS

  • Zaharescu, Alexandru
    • 대한수학회보
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    • 제40권3호
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    • pp.509-520
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    • 2003
  • Let p be a prime number, $Q_{p}$ the field of p-adic numbers, K a finite extension of $Q_{p}$, $\bar{K}}$ a fixed algebraic closure of K and $C_{p}$ the completion of K with respect to the p-adic valuation. Let E be a closed subfield of $C_{p}$, containing K. Given elements $t_1$...,$t_{r}$ $\in$ E for which the field K($t_1$...,$t_{r}$) is dense in E, we construct integral bases of E over K.

아동의 복합운동이 착지 시 하지 손상요인에 미치는 영향 (Effects of Combined Exercise on Injury Risk Factors of Lower Extremity during Landing)

  • 하성희;류시현;김주년;길호종;류지선;윤석훈;박상균
    • 한국운동역학회지
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    • 제24권2호
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    • pp.173-180
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    • 2014
  • The purpose of this study was to investigate the effect of combined exercise on injury risk factors of lower extremity during landing. Ten sports talented athletes participated in this study. Sports talented athletes participated in a combined exercise (sports talented exercise, coordination) for 16 weeks. A three-dimensional motion analysis was performed using eight infrared cameras (sampling rate of 100 Hz), one force plate, and electromyography system (sampling rate of 1000 Hz) during landing. Kinetic, and kinematics analysis including average impulsive force, angle of lower extremity, vertical stiffness, onset of muscle activation were calculated by Matlab2009a software. Paired t-test was performed at alpha=.05. The average impulsive force in landing phase was not statistically significant (t=-.748, p=.474). The hip joint angle was more decreased in post test compared to pre test (E1: t=2.682, p=.025, E2: t=5.609, p=.000, E3: t=2.538, p=.032). The knee joint (E1: t=-.343, p=.739, E2: t=1.319, p=.220, E3: t=.589, p=.570) and ankle joint (E1: t=.081, p=.937, E2: t=.784, p=.453, E3: t=.392, p=.704) angle were tended to decrease after combined exercise. The vertical stiffness was tended to decrease after combined exercise (t=1.972, p=.080). Onset of quadriceps femoris (t=.698, p=.503) and medial gastocnemius (t=1.858, p=.096) were tended to be faster than biceps femoris (t=-.333, p=.747) after combined exercise. Although thses findings were not statistically significant except on a hip joint angle, risk factors of lower extremity such as joint angle, vertical stiffness and onset of quadriceps femoris, medial gastrocnemius were positively changed after the combined exercise but an additional training for improved onset of biceps femoris would be required in the future.

광양만에 분포하는 농조개, Paphia undalata (Born)의 연령과 성장에 관한 기초연구 (Preliminary Survey of Age and Growth of the Short-necked Clam, Paphia undalate(Born), in Kwangyang Bay, Korea)

  • 김영혜;장대수;박영철
    • 한국패류학회지
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    • 제17권1호
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    • pp.7-12
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    • 2001
  • 전라남도 광양시 송도 주변해역에서 2000년 12월에 형망에 의해 채집한 농조개의 연령과 성장을 조사하였다. 연령형질은 패각을 사용하였으며, 윤문은 패각에 나타난 불투명대에서 투명대로 이행하는 경계로 하였다. 출현한 개체의 각장은 13.1-44.2mm이었고, 평균은 19.6mm이었으며, 연령은 2세까지 출현하였다. 각장 (SL) 과 각고 (SH) 간의 상대성장은 SL = 0.2105 + 1.7569 $\times$ SH이었고, 각장 (SL)과 전중 (TW) 간의 상대성장은 TW = 2.5824$\times$ $10^{-4}$ $\times$ S $L^{2.6769}$이었다. Von Bertalanffy 성장식은 $L_{t}$ = 81.46(1-e $^{-0}$.20(t+1.19)/), $W_{t}$ = 33.68(1-e $^{0.20(t+1.19)}$)$^{2.6769}$ 추정되었으며, 극한 각장 ( $L_{\infty}$) 은 81.46 mm, 성장계수 (K) 0.20/year 이었다. 0.20/year 이었다..

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Signal Transduction of C-Terminal Phosphorylation Regions for Equine Luteinizing Hormone/Chorionic Gonadotropin Receptor (eLH/CGR)

  • Byambaragchaa, Munkhzaya;Joo, Hyo-Eun;Kim, Sang-Gwon;Kim, Yean-Ji;Park, Gyeong-Eun;Min, Kwan-Sik
    • 한국발생생물학회지:발생과생식
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    • 제26권1호
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    • pp.1-12
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    • 2022
  • This study aimed to investigate the signal transduction of phosphorylation sites at the carboxyl (C)-terminal region of equine luteinizing hormone/chorionic gonadotropin receptor (eLH/CGR). The eLH/CGR has a large extracellular domain of glycoprotein hormone receptors within the G protein-coupled receptors. We constructed a mutant (eLH/CGR-t656) of eLH/CGR, in which the C-terminal cytoplasmic tail was truncated at the Phe656 residue, through polymerase chain reaction. The eLH/CGR-t656 removed 14 potential phosphorylation sites in the intracellular C-terminal region. The plasmids were transfected into Chinese hamster ovary (CHO)-K1 and PathHunter Parental cells expressing β-arrestin, and agonist-induced cAMP responsiveness was analyzed. In CHO-K1 cells, those expressing eLH/CGR-t656 were lower than those expressing eLH/CGR wild-type (eLH/CGR-wt). The EC50 of the eLH/CGR-t656 mutant was approximately 72.2% of the expression observed in eLH/CGR-wt. The maximal response in eLH/CGR-t656 also decreased to approximately 43% of that observed in eLH/CGR-wt. However, in PathHunter Parental cells, cAMP activity and maximal response of the eLH/CGR-t656 mutant were approximately 173.5% and 100.8%, respectively, of that of eLH/CGR-wt. These results provide evidence that the signal transduction of C-terminal phosphorylation in eLH/CGR plays a pivotal role in CHO-K1 cells. The cAMP level was recovered in PathHunter Parental cells expressing β-arrestin. We suggest that the signal transduction of the C-terminal region phosphorylation sites is remarkably different depending on the cells expressing β-arrestin in CHO-K1 cells.

한국 멸치어업의 어획량 분석과 예측 ARIMA 모델 및 스펙트럼 해석 (Analysis and Prediction of Anchovy Fisheries in Korea ARIMA Model and Spectrum Analysis)

  • 박해훈;윤갑동
    • 한국수산과학회지
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    • 제29권2호
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    • pp.143-149
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    • 1996
  • 우리나라 멸치어업에서의 1971~1992년 동안의 22년간 월별 어획량 자료를 시계열 분석하여 어획량을 분석, 예측하였다. 시계열 분석은 다른 생물학적, 해양학적, 사회 경제적인 요소가 없어도 단지 어획량 자료만으로 분석과 예측이 가능하다. 첫 20년간인 1971~1990년 사이의 월별 멸치 어획량 자료를 ARIMA 시계열 모형에 적용시켜 구한 결과는 다음과 같다. 로그 (대수) 변환시켰을 때의 ARIMA 모형: $$(1-0.381B)(1-0.027B^{12}+0.141B^{24})(1-B^1)(1-B^{12})Z_t=(1-0.968B)(1-0.727B^{12})e_t$$, Box-Cox 변환시켰을 때의 ARIMA 모형: $$(1-0.431B)(1-B^{12})Z_t=(1-0.882B^{12})e_t$$, 위의 두 모형중 Box-Cox 변환시킨 것이 로그 (대수) 변환시킨 것보다 예측오차가 적었으며, Box-Cox 변환식은 $Y'=(Y^{0.58}-1)/0.58$ 이었다. 위의 두 모형 중 후자의 모형을 이용하여 1991~1992년 사이의 월별 어획량을 예측하였다. 예측 어획량과 실제 어획량과의 월별 오차범위는 1.0~63.2% (1991년에 1.6~63.2%이고, 1992년에는 1.0~60.4%)였다. 예측 어획량이 각 연도별로 148,201M/T과 148,834M/T인데 비해, 실제 어획량은 170,293M/T, 168,234M/T이었다. 2년 동안의 총어획량에 대한 오차는 12.3%였다. 또한 스펙트럼 분석은 순환변동의 주기가 2.2개월, 6.1개월, 10.2개월, 12개월, 14.7개월에서 상대적으로 큰 성분이 있음을 나타내었다 이 순환변동 성분은 적절한 ARIMA 모형을 결정하는 데도 도움이 된다.

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Nitric Oxide-Induced Autophagy in MC3T3-E1 Cells is Associated with Cytoprotection via AMPK Activation

  • Yang, Jung Yoon;Park, Min Young;Park, Sam Young;Yoo, Hong Il;Kim, Min Seok;Kim, Jae Hyung;Kim, Won Jae;Jung, Ji Yeon
    • The Korean Journal of Physiology and Pharmacology
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    • 제19권6호
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    • pp.507-514
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    • 2015
  • Nitric oxide (NO) is important in the regulation of bone remodeling, whereas high concentration of NO promotes cell death of osteoblast. However, it is not clear yet whether NO-induced autophagy is implicated in cell death or survival of osteoblast. The present study is aimed to examine the role of NO-induced autophagy in the MC3T3-E1 cells and their underlying molecular mechanism. The effect of sodium nitroprusside (SNP), an NO donor, on the cytotoxicity of the MC3T3-E1 cells was determined by MTT assay and expression of apoptosis or autophagy associated molecules was evaluated by western blot analysis. The morphological observation of autophagy and apoptosis by acridine orange stain and TUNEL assay were performed, respectively. Treatment of SNP decreased the cell viability of the MC3T3-E1 cells in dose- and time-dependent manner. SNP increased expression levels of p62, ATG7, Beclin-1 and LC3-II, as typical autophagic markers and augmented acidic autophagolysosomal vacuoles, detected by acridine orange staining. However, pretreatment with 3-methyladenine (3MA), the specific inhibitor for autophagy, decreased cell viability, whereas increased the cleavage of PARP and caspase-3 in the SNP-treated MC3T3-E1 cells. AMP-activated protein kinase (AMPK), a major autophagy regulatory kinase, was activated in SNP-treated MC3T3-E1 cells. In addition, pretreatment with compound C, an inhibitor of AMPK, decreased cell viability, whereas increased the number of apoptotic cells, cleaved PARP and caspase-3 levels compared to those of SNP-treated MC3T3-E1 cells. Taken together, it is speculated that NO-induced autophagy functions as a survival mechanism via AMPK activation against apoptosis in the MC3T3-E1 cells.

양식넙치(Paralichthys olivaceus)의 복수증에 관한 연구 - Edwardsiella tarde의 일부 특성과 병원성 및 대책 - (Studies on the Edwardsiellosis of cultured flounder, Paralichthys oliuaceus;- Characteristics, pathgenicity and control of Edwardsiella tarda -)

  • 한재철;김영진;서형석;김영길;이근광;안병목
    • 한국동물위생학회지
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    • 제20권1호
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    • pp.11-18
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    • 1997
  • Characteristics, pathogenicity and control of the causative organisms isolated front diseased cultured flounder, Paralichthys olivaceus were studied. The causative organisms were identified as E tarda by biochemical and biophysical characteristics. Also, it strains were named as E tarda KBF-1 and E tarda KMF-1, and optimal pH of E tarda KBF-1 and E tarda KMF-1 were 8.0, and optimal concentration of NaCl. E tarda KBF-1 was 0% and E tarda KMF-1 was 1%. In the pathogenicity test, 0~10 of the flounders of artificially infected group(E tarda KBF-1) with $1.0{\times}10^7$ cfu/fish were died within 60 hrs, but 0~9 flounders infected group with 41.0{\times}10^6$ cfu/fish were died within 60 hrs. Also, 0~10 flounders infected group(E tarda KMF-1) with $1.0{\times}10^7$ cfu/fish were died within 36 hrs, while 0~7 flounders infected with $1.0{\times}10^6$ cfu/fish were died within 60 hrs. Drug sensitivity of E tarda KBF-1 strain was resistant to AM, CF and N, and intermediate to E, K and S, and sensitivity to C, G, SxT and FF. But E tarda KMF-1 strain was resistant to CF, E and V, and intermediate to AM, C, N and SxT, and sensitivity to GM and FF.

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MC3T3-E1 세포에서 BMP2에 의한 조골세포의 분화에 일주기 유전자 Per1이 미치는 영향 (Circadian Clock Gene Per1 Mediates BMP2-induced Osteoblast Differentiation in MC3T3-E1 Cells)

  • 민현영;장원구
    • 생명과학회지
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    • 제27권5호
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    • pp.501-508
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    • 2017
  • Bone morphogenetic proteins (BMPs)는 다양한 세포기능을 조절하는 중요한 사이토카인 중 하나이다. 최근 BMP와 일주기 유전자들이 연관되어 있다는 연구결과들이 보고되고 있지만 조골세포에서 일주기 유전자인 Per1의 역할은 아직 명확하지 않다. 본 연구에서는 조골세포 분화에서 Per1의 역할을 조사하였다. MC3T3-E1 세포에서 BMP2 처리에 의해 Per1 mRNA 발현과 luciferase 활성이 증가하는 것을 확인하였다. 또한 Per1 과발현 실험을 통해서 Per1 유전자가 Runx2, ALP, OC의 발현을 증가시켰으며 ascorbic acid와 ${\beta}$-glycerophosphate에 의한 ALP 염색과 석회화가 Per1 과발현에 의해 더욱 증가하는 것을 확인하였다. 이상의 결과는 일주기 리듬을 조절하는 Per1 유전자가 조골세포의 분화를 촉진하는 인자로 작용함을 시사한다.