• IMMUNE NETWORK
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• v.21 no.4
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• pp.28.1-28.14
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• 2021

Lung-resident memory T cells (T_RM) play an essential role in protecting against pulmonary virus infection. Parenteral administration of DNA vaccine is generally not sufficient to induce lung CD8 T_RM cells. This study investigates whether intramuscularly administered DNA vaccine expressing the nucleoprotein (NP) induces lung T_RM cells and protects against the influenza B virus. The results show that DNA vaccination poorly generates lung T_RM cells and massive secondary effector CD8 T cells entering the lungs after challenge infection do not offer sufficient protection. Nonetheless, intranasal administration of non-replicating adenovirus vector expressing no Ag following priming DNA vaccination deploys NP-specific CD8 T_RM cells in the lungs, which subsequently offers complete protection. This novel 'prime and deploy' strategy could be a promising regimen for a universal influenza vaccine targeting the conserved NP Ag.

• Journal of Microbiology and Biotechnology
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• v.34 no.2
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• pp.280-288
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• 2024

The fusogenic membrane glycoprotein (FMG) derived from the human endogenous retrovirus-W (HERV-W) exhibits fusogenic properties, making it a promising candidate for cancer gene therapy. When cells are transfected with HERV-W FMG, they can fuse with neighboring cells expressing the receptor, resulting in the formation of syncytia. These syncytia eventually undergo cell death within a few days. In addition, it has been observed that an HERV-W env mutant, which is truncated after amino acid 483, displays increased fusogenicity compared to the wild-type HERV-W env. In this study, we observed syncytium formation upon transfection of HeLa and TE671 human cancer cells with plasmids containing the HERV-W 483 gene. To explore the potential of a semi-replication-competent retroviral (s-RCR) vector encoding HERV-W 483 for FMG-mediated cancer gene therapy, we developed two replication-defective retroviral vectors: a gag-pol vector encoding HERV-W 483 (MoMLV-HERV-W 483) and an env vector encoding VSV-G (pCLXSN-VSV-G-EGFP). When MoMLV-HERV-W 483 and pCLXSN-VSV-G-EGFP were co-transfected into HEK293T cells to produce the s-RCR vector, gradual syncytium formation was observed. However, the titers of the s-RCR virus remained consistently low. To enhance gene transfer efficiency, we constructed an RCR vector encoding HERV-W 483 (MoMLV-10A1-HERV-W 483), which demonstrated replication ability in HEK293T cells. Infection of A549 and HT1080 human cancer cell lines with this RCR vector induced syncytium formation and subsequent cell death. Consequently, both the s-RCR vector and RCR encoding HERV-W 483 hold promise as valuable tools for cancer gene therapy.

• Korean Journal of Veterinary Research
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• v.52 no.3
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• pp.183-191
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• 2012

The maintenance of peripheral immune tolerance and prevention of chronic inflammation and autoimmune disease require $CD4^{+}CD25^{+}$ T cells (regulatory T cells). The transcription factor Foxp3 is essential for the development of functional, regulatory T cells, which plays a prominent role in self-tolerance. Retroviral vectors can confer high level of gene transfer and transgene expression in a variety of cell types. Here we observed that following retroviral vector-mediated gene transfer of Foxp3, transductional Foxp3 expression was increased in the liver, lung, brain, heart, muscle, spinal cord, kidney and spleen. One day after vector administration, high levels of transgene and gene expression were observed in liver and lung. At 2 days after injection, transductional Foxp3 expression level was increased in brain, heart, muscle and spinal cord, but kidney and spleen exhibited a consistent low level. This finding was inconsistent with the increase in both $CD4^{+}CD25^{+}$ T cell and $CD4^{+}Foxp3^{+}$ T cell frequencies observed in peripheral immune cells by fluorescence-activated cell-sorting (FACS) analysis. Retroviral vector-mediated gene transfer of Foxp3 did not lead to increased numbers of $CD4^{+}CD25^{+}$ T cell and $CD4^{+}Foxp3^{+}$ T cell. These results demonstrate the level and duration of transductional Foxp3 gene expression in various tissues. A better understanding of Foxp3 regulation can be useful in dissecting the cause of regulatory T cells dysfunction in several autoimmune diseases and raise the possibility of enhancing suppressive functions of regulatory T cells for therapeutic purposes.

• Parasites, Hosts and Diseases
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• v.56 no.5
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• pp.515-519
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• 2018

Triatoma rubrofasciata is a wide-spread vector of Chagas disease in Americas. In this study, we completed the mitochondrial genome sequencing of T. rubrofasciata. The total length of T. rubrofasciata mitochondrial genome was 17,150 bp with the base composition of 40.4% A, 11.6% G, 29.4% T and 18.6% C. It included 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes and one control region. We constructed a phylogenetic tree on the 13 protein-coding genes of T. rubrofasciata and other 13 closely related species to show their phylogenic relationship. The determination of T. rubrofasciata mitogenome would play an important role in understanding the genetic diversity and evolution of triatomine bugs.

• Journal of the Korean Mathematical Society
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• v.55 no.3
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• pp.593-604
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• 2018

Recently, a new version of expansiveness which is closely attached to some certain weak version of hyperbolicity was given for $C^1$ vector fields as following: a $C^1$ vector field X will be called rescaling expansive on a compact invariant set ${\Lambda}$ of X if for any ${\epsilon}$ > 0 there is ${\delta}$ > 0 such that, for any $x,\;y{\in}{\Lambda}$ and any time reparametrization ${\theta}:{\mathbb{R}}{\rightarrow}{\mathbb{R}}$, if $d({\varphi}_t(x),\,{\varphi}_{{\theta}(t)}(y)){\leq}{\delta}{\parallel}X({\varphi}_t(x)){\parallel}$ for all $t{\in}{\mathbb{R}}$, then ${\varphi}_{{\theta}(t)}(y){\in}{\varphi}_{(-{\epsilon},{\epsilon})}({\varphi}_t(x))$ for all $t{\in}{\mathbb{R}}$. In this paper, some equivalent definitions for rescaled expansiveness are given.

• Proceedings of the Korean Society of Computer Information Conference
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• 2017.01a
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• pp.73-74
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• 2017

SVM(Support Vector Machine) 알고리즘은 대표적인 기계 학습 분류 알고리즘으로 감정 분석, 제스처 인식 등 다양한 분야의 문제를 해결하기 위해 사용되고 있다. SVM 알고리즘은 분리경계면(Hyper-Plane) 또는 분리경계면 집합 중 지지벡터(Support Vector)라 불리는 특정한 점들로 이루어진 두 그룹 간의 거리 차이(Margin)를 최대로 하는 분리경계면을 이용하여 데이터를 분류하는 알고리즘이다. 높은 정확도를 제공하지만 처리 속도가 느리며 학습을 위해 대량의 데이터 및 메모리가 필요하기 때문에 자원이 제한적인 IoT 환경에서 사용이 어렵다. 본 논문에서는 자원이 제한된 IoT 노드를 기반으로 효율적으로 데이터를 학습하기 위해 K-means 알고리즘을 이용하여 SVM 알고리즘의 저전력화 방안을 연구한다.

• Journal of Advanced Marine Engineering and Technology
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• v.28 no.2
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• pp.185-191
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• 2004

This study describes the scheme of vector drive system without speed sensor for AC servo motor using theory of a flux observer and based on the field oriented vector control. The new method of speed estimation is presented from operate with the position and magnitude of the secondary flux which obtain from the voltage reference and detected current. As the estimated speed is settled by the flux and the machine-specific parameters. this method don't need to adjust the gain of the parameter. Based on the derived theory for vector control. the scheme for sensorless vector drive of AC servo motor is designed and realized. And the experiment verifies it passable to realize the sensorless vector drive based on a field-oriented type.

• Journal of applied mathematics & informatics
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• v.28 no.3_4
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• pp.697-710
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• 2010

In this paper, we prove existence of infinitely many positive and concave solutions, by means of a simple approach, to $3^{th}$ order three-point singular boundary value problem {$x^{\prime\prime\prime}(t)=\alpha(t)f(t,x(t))$, 0 < t < 1, $x(0)=x'(\eta)=x^{\prime\prime}(1)=0$, (1/2 < $\eta$ < 1). Moreover with respect to multiplicity of solutions, we don't assume any monotonicity on the nonlinearity. We rely on a combination of the analysis of the corresponding vector field on the phase-space along with Knesser's type properties of the solutions funnel and the well-known Krasnosel'ski$\breve{i}$'s fixed point theorem. The later is applied on a new very simple cone K, just on the plane $R^2$. These extensions justify the efficiency of our new approach compared to the commonly used one, where the cone $K\;{\subset}\;C$ ([0, 1], $\mathbb{R}$) and the existence of a positive Green's function is a necessity.

• Journal of the Korean Mathematical Society
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• v.45 no.5
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• pp.1443-1482
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• 2008

In this paper we define a Myller configuration in a Finsler space and use some special configurations to obtain results about Finsler subspaces. Let $F^{n}$ = (M,F) be a Finsler space, with M a real, differentiable manifold of dimension n. Using the pull back bundle $({\pi}^{*}TM,\tilde{\pi},\widetilde{TM})$ of the tangent bundle $(TM,{\pi},M)$ by the mapping $\tilde{\pi}={\pi}/TM$ and the Cartan Finsler connection of a Finsler space, we obtain an orthonormal frame of sections of ${\pi}^{*}TM$ along a regular curve in $\widetilde{TM}$ and a system of invariants, geometrically associated to the Myller configuration. The fundamental equations are written in a very simple form and we prove a fundamental theorem. Important lines in a Finsler subspace are defined like special lines in a Myller configuration, geometrically associated to the subspace: auto parallels, lines of curvature, asymptotes. Torse forming vector fields with respect to the Cartan Finsler connection are characterized by means of the invariants of the Frenet frame of a versor field along a curve, and the new notion of torse forming vector fields in the sense of Myller is introduced. The particular cases of concurrence and parallelism in the sense of Myller are completely studied, for vector fields from the distribution $T^m$ of the Myller configuration and also from the normal distribution $T^p$.

• The Journal of Fisheries Business Administration
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• v.46 no.1
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• pp.93-107
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• 2015

This study aims to analyze causalities among Hairtail prices by distribution channel using a vector autoregressive model. This study applies unit-root test for stability of data, uses Granger causality test to know interaction among Hairtail Prices by distribution channel, and employes the vector autoregressive model to estimate statistical impacts among t-2 period variables used in model. Analyzing results of this study are as follows. First, ADF, PP, and KPSS tests show that the change rate of Hairtail price by distribution channel differentiated by logarithm is stable. Second, a Granger causality test presents that the producer price of Hairtail leads the wholesale price and then the wholesale price leads the consumer price. Third, the vector autoregressive model suggests that the change rate of Hairtail producer price of t-2 period variables statistically, significantly impacts change rates of own, wholesale, and consumer prices at current period. Fourth, the impulse response analysis indicates that impulse responses of the structural shocks with a respectively distribution channel of the Hairtail prices are relatively more powerful in own distribution channel than in other distribution channels. Fifth, a forecast error variance decomposition of the Hairtail prices points out that the own price has relatively more powerful influence than other prices.