• Journal of Animal Science and Technology
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• v.47 no.4
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• pp.491-500
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• 2005

To test applicability to the Hanwoo traceability system, twenty microsatellite markers were selected and analyzed. MSA, CERVUS, FSTAT, GENEPOP, API_CALC and PHYLIP software was employed serially to estimate heterozygosity, polymorphic information content, F-statistics, identity probability, exclusion probability and genetic distance. Eleven microsatellite markers(TGLA53, TGLA227, ETH185, TGLA122, BM4305, INRA23, ILSTS013, BMS1747, BM2113, BL1009, and ETH3) were selected based on their high heterozygosity values. Identity probability using these markers is one hundred times higher than when using StockMakersTM of Applied Biosystems. This indicates the selected microsatellite markers are appropriate and effective for use in the Hanwoo traceability system. Additionally, estimates of DA genetic distance and pairwise-FST can be utilized to identify genetic relationships between adjacent farms.

• Journal of Veterinary Clinics
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• v.26 no.5
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• pp.419-422
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• 2009

We performed the PARR (PCR to detect antigen receptor rearrangements) test on DNA isolated from twelve archival canine cytological slides including nine lymphoma, two reactive lymphocytes and one sample from Ehrlichia canis infected dog. As a result, our PCR control gene, $C{\mu}$, was successfully amplified from all of the DNA samples. Six out of nine lymphoma samples showed a clonal rearrangement of immunoglobulin gene whereas three samples did a clonal rearrangement of T cell receptor gamma ($TCR{\gamma}$) gene. However, we observed no visible or clear bands from PCR conducted using our antigen receptor rearrangement primers on DNA from a reactive lymphoid cell proliferation used as a negative control. False-positive amplification in $TCR{\gamma}$ gene was observed only in one sample from E. canis infection. The use of archival cytological specimens demonstrated in this study offers potential advantages for cost-effective specimen acquisition and efficient high-fidelity DNA analysis.

• International Journal of Highway Engineering
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• v.10 no.4
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• pp.1-8
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• 2008

Seoul metropolitan government has introduced Bus Rapid Transit (BRT) systems in some major urban corridors on July 1st, 2004, and has reported that it was successful on the ground that bus speed has been increased in the corridors with BRT system and that it has also resulted in the increase of bus patrons. However, there comes a negative claim that BRT has increased the number of fatalities in the same corridors. This study first aims at developing safety evaluation indicators of pedestrian crossings, then compares values of those indicators from pedestrian crossings with and without BRT systems. The safety evaluation indicators of pedestrian crossing are developed as: the number (or ratio) of conflicts between pedestrians and vehicles, the number (or ratio) of vehicles violating pedestrian signals, the number (or ratio) of pedestrians remaining crossings at red signals, and the number (ratio) of pedestrians walking beyond crossing areas. When we compare values of these indicators for both pedestrian crossings with and without BRT system, it has turned out that crossings with BRT systems has more number of conflicts than the crossings without BRT systems, but it cannot be said that the ratio of conflicts will be different between two crossing types. It means that the number of conflict will increase as the number of pedestrians and vehicles in the crossings. When we note that BRT system intrinsically attract more number of pedestrians who get-in or get-off bus stops, we cannot said that BRT system will be safer for pedestrians.

• Journal of Korean society of Dental Hygiene
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• v.10 no.6
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• pp.1107-1119
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• 2010

Objectivs : The purpose of this study is to provide basic data for the use of oral hygiene devices and to improve oral health care continuously by encouraging the use of oral hygiene devices. Methods : The survey was carried out by questionnaire research targeting 395 respondents in metropolitan area in June-August 2009. The collected data is analyzed by statistics program SPSS version 12.0. 1. General characteristics of subjects are analyzed by percentage and descriptive statistics. 2. Recognition about how to use oral hygiene devices, usage of oral hygiene devices, recognition of dental brush usage, Effect after using oral hygiene devices are analyzed by mean difference test(t, F verification). 3. Effect after using oral hygiene devices, relation of oral hygiene devices usage with subjective oral health status, oral hygiene devices usage and participation in education are analyzed by Correlation, Regression analysis. Results : 1. Investigation shows that most oral health devices are rarely used except toothbrush(4.57) in case of the use of oral hygiene devices. 2. In the case of awareness of oral hygiene devices usage, almost everyone knows how to use dental brushes(MEAN 4.16) the most. And the next ones are like these-tooth picks(MEAN 3.38), dental floss(MEAN 3.28), mouth rinse(MEAN 3.24). And they don't know how to use the other oral hygiene devices usage. 3. respondents who use dental floss(${\beta}$=.238, p<.001), interdental brush(${\beta}$=.242, p<.001) and water pik(${\beta}$ =.180, p<.05) recognize that the result and effect after using them are more positive than the others in different case. 4. Respondents who use toothbrush(${\beta}$=.119), dental floss(${\beta}$=.120) and interdental brush(${\beta}$=.136). frequently evaluate their subjective oral health status positively in case of frequency of using oral hygiene devices and subjective oral health status(p<.05). 5. Respondents who use toothbrush and interdental brush properly and frequently, who are older than the others show more concern about participating in education of "How to use oral hygiene devices" than the others(p<.05). Conclusions : In conclusion, to prevent oral disease, the opportunity of participating in education about correct knowledge and proper way should be expanded. And we should contribute to oral health improvement through developing, executing and informing systematic, popular oral health education with active one.

• Journal of Hospice and Palliative Care
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• v.7 no.2
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• pp.179-188
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• 2004

Purpose: The purpose of this study was to investigate the degree of fatigue and its related factors in cancer patients during chemotherapy. Methods: The subjects of this study consisted of 90 patients over 20 years old who were receiving chemotherapy at the injection room of the o.p.d. and ward admission care unit in a University hospital located in Gwang-ju city and data were collected from August 8th to October 2nd, 2002. Collected data were analysed using SPSS v 10.0. to obtain summary statistics for the descriptive analysis, t-test, ANOVA, pearson correlation, and multiple regression. Results: 1. Fatigue of the subjects was significantly correlated with physical distress score. and 6 items of subscale those were nausea, vomiting, anorexia, pain, and immobility, showed statistically significant correlation. 2. Fatigue of the subjects showed statistically significant differences according to a nap satisfaction. Fatigue of the subjects was significantly correlated with mood state, Also, all 5 items of subscale, which are those were anxiety, confusion, depression, energy, and anger showed statistically significant correlations. 3. Fatigue of the subjects showed statistically significant differences according to metastasis, chemotherapy cycle, post operation existence, post radiation therapy existence. There were significant negative correlation between fatigue and hematocrit and fatigue and weight change. There was no significant correlation between fatigue and spiritual well-being state. With the result to multiple regression, Immobility, Anorexia, Anger explained fatigue by, pain, and immobility showed statistically significant correlation.

• Microbiology and Biotechnology Letters
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• v.31 no.2
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• pp.103-110
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• 2003

The regulation of pyrimidine nucleotide synthesis has been proved to be controlled by a regulatory protein PyrR-mediated attenuation in the Gram-positive bacteria. After several bacterial genome sequencing projects, we have discovered the PyrR orthologues in the databases for Haemophilus influenzae and Synechocystis and sp. PCC6803 genome sequences. To investigate whether these PyrR orthologue proteins regulate pyrimidine nucleotide synthesis as well as the cases of Bacillus, the PyrR regions of each strains were amplified by PCR and cloned with pUC19 or T-vector in Escherichia coli and with a shuttle vector pHPS9 for E. coli and B. subtilis. For the regulation test of the PyrR orthologues, the aspartate-transcarbamylase (ATCase) assay was carried out. From the results of the ATCase assay, it was confirmed that Synechocystis sp. PCC6803 could not restore by pyrimidines to a B. subtilis, PyrR but H. influenzae PyrR could. For Purification of PyrR orthologue proteins, PyrR orthologue genes were cloned into the expression vector (pET14b). Over-expressed product of PyrR orthologue genes was purified and analyzed by the SDS-PACE. The purified PyrR orthologue proteins from H. influenzae and Synechocystis sp. PCC6803 turned out to be molecular mass of 18 kDa and 21 kDa, respectively. The result of uracil phosphoribosyl transferase (UPRTase) assay with purified PyrR orthologue proteins showed that H. influenzae PyrR protein only has UPRTase activity. In addition, we could predict several regulatory mechanisms that PyrR orthologue proteins regulate pyrimidine de novo synthesis in bacteria, through phylogenetic analysis for PyrR orthologue protein sequences.

• Korean Journal of Social Welfare
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• v.60 no.3
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• pp.253-274
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• 2008

The link between work life and family life is an essential subject matter in understanding the lives of dual-earner couples. Concepts of spillover and crossover explain the link between work and family. The present study examines both the positive and negative aspects in these processes. The data come from the Sloan Working Families Study conducted by the Alfred P. Sloan Center on Parents, Children, and Work and NORC at the University of Chicago. The Experience Sampling Method employed explores directly the daily life experiences of the participants. The data were analysed using t-test. Both spillover and crossover were found in the lives of dual-earner couples in this study. Men and women brought happy emotions at work to home, but the data provide limited support for spillover of negative emotions. Gender differences were more apparent in examining the process of crossover. Men appear responsive to the positive and negative emotional experiences their spouse brought home while women were found not to be responsive to their spouses' positive emotional experiences at work. Furthermore, the analysis revealed an interesting trend concerning the emotions of working couples in that they generally seem to recover to their average level of emotions once home. This suggests that home can be a respite from strong emotions, a comforting place. By looking closely into the emotions experienced by working parents in their daily lives, this study adds contextual understanding concerning the link between work and family life. The findings on the effects of positive experiences at work invite social work practitioners and researchers to further investigate the phenomena of spillover and crossover processed in greater detail, taking into account this contextual aspect of family life as well as the work life of dual-earner couples.

• Journal of Bio-Environment Control
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• v.29 no.3
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• pp.259-264
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• 2020

In this study, the humidity control effect of a counter-flow ventilator was analyzed in a greenhouse with high relative humidity at night in the winter season. A case of the counter-flow ventilator was 0.96 × 0.65× 0.82(W × D × H, m) and there were heat transfer element and two fans for air supply and exhaust in the counter-flow ventilator. Two counter-flow ventilators were used in this study and the setting humidity of the ventilators was 80%. The temperature and relative humidity at night(18:00-8:00) in the greenhouse were measured. In a greenhouse without a counter-flow ventilator, the average temperature and humidity was 14.9℃, 82.8%, respectively. When the counter-flow ventilator was operated, the corresponding averages were 15.1℃, 79.9%. The independent sample t test of monthly temperature and relative humidity showed no difference in temperature, and a significant difference in relative humidity with 1% of the significance level. Therefore, using the counter-flow ventilator helps to control relative humidity in greenhouse and increase yield.. And further research considering the pros and cons of using the counter-flow ventilator is needed.

• Journal of the Korean Society of Marine Environment & Safety
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• v.21 no.6
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• pp.696-703
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• 2015

This paper is a follow-up research of 'A study on the development of Safety Management Evaluation Index for Shipping Company'. It aims to develop the evaluation items of Safety Management Index(hereafter called as 'SMI') applicable to 'Safety Advantage Point', which is suggested as the sub-index of 'Safety Management Evaluation Index' function in the preceding research and also verify their adequacy. For that purpose, firstly, the 11 evaluation items were developed after collecting the practical opinions from safety management personnels in Korean shipping companies. Secondly, the survey was carried out for the verification of the adequacy of the 11 evaluation items. And lastly, its results was analysed, whether it has statistical meanings of differences in adequacy awareness amongst safety managers grouped depending on business type(coastal, ocean-going) and business capacity(number of owned-operating ships). As a result, there was no relevance between their adequacy awareness and business type of their companies. However, a difference in their adequacy awareness existed depending on their companies' business capacity(small, medium and large sized). In conclusion, when the safety management level of shipping companies is evaluated by SMI, the same indices can be applicable to both coastal and ocean-going shipping companies by a grouping of companies' business capacity.

• Journal of Industrial Convergence
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• v.18 no.1
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• pp.45-52
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• 2020

This study is a descriptive study conducted to investigate the effect of smoking cigarette sign sold in Korea on the smoking cessation effects and to use it as a basis for future smoking cessation and health related health data. To this end, we surveyed 500 men and women over 20 years of age on the Internet Naver web using Naver form questionnaire for 10 days from October 1, 2018 using blogs and cafes. The analyzed data were analyzed by factor analysis, T-test and correlation using SPSS WIN20.0. The main findings were as follows: First, the cigarette warning picture harmful to human body had the biggest influence on the smoking cessation effect. Second, the smoking cessation effect had the highest correlation with the pre-question consciousness about the cigarette warning picture. This indicates that the cigarette pack warning is considered the most sensitive part of smokers. Based on the results of this paper, diversification of cigarette warning picture and regular replacement of warning picture are expected to provide useful basic data for suggesting practical measures to prevent smoking. do. Therefore, the smoking warning picture is widely promoted through the media and the media, which has a positive effect on the practice of quitting smoking to the public, and is also used as a basic data for the health policy for the health promotion of the public.