Journal of Veterinary Clinics (한국임상수의학회지)

The Korean Society of Veterinary Clinics (한국임상수의학회)
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Detection of Canine Lymphoma by the Amplification of Antigen Receptor Gene Rearrangements

재배열 항원 수용체 유전자 증폭을 통한 개 림프종의 진단

  • Yu, Do-Hyeon (College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University) ;
  • Li, Ying-Hua (College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University) ;
  • Lee, Jong-Hyun (College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University) ;
  • Noh, Dong-Ho (College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University) ;
  • Song, Ru-Hui (College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University) ;
  • Lee, Mi-Jin (College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University) ;
  • Choi, Ul-Soo (College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University) ;
  • Park, Jin-Ho (College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University)
  • Published : 2009.10.31
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Abstract

We performed the PARR (PCR to detect antigen receptor rearrangements) test on DNA isolated from twelve archival canine cytological slides including nine lymphoma, two reactive lymphocytes and one sample from Ehrlichia canis infected dog. As a result, our PCR control gene, $C{\mu}$, was successfully amplified from all of the DNA samples. Six out of nine lymphoma samples showed a clonal rearrangement of immunoglobulin gene whereas three samples did a clonal rearrangement of T cell receptor gamma ($TCR{\gamma}$) gene. However, we observed no visible or clear bands from PCR conducted using our antigen receptor rearrangement primers on DNA from a reactive lymphoid cell proliferation used as a negative control. False-positive amplification in $TCR{\gamma}$ gene was observed only in one sample from E. canis infection. The use of archival cytological specimens demonstrated in this study offers potential advantages for cost-effective specimen acquisition and efficient high-fidelity DNA analysis.

개의 림프종을 분자유전자학적으로 진단하고자 본 연구를 수행하였다. 이를 위하여 12개의 염색 및 고정된 도말 표본으로부터 DNA를 추출한 후, 임파육종에 특이적인 재배열된 항원 수용체 유전자를 중합효소연쇄반응으로 증폭시켰다. 그 결과 6개의 type-B 림프종에서 type-B 세포의 림프종에 특이적인 IgH (immunoglobulin H) 주유전자(major gene)와 type-B 림프종의 부유전자(minor gene)가 증폭되었다. 또한, 3개의 type-T 세포의 림프종의 경우에도 이에 특이적인 $TCR{\gamma}$ 유전자가 증폭되었다. 한편, 에를리키아증에 이환된 표본은 $TCR{\gamma}$ 유전자가 위양성으로 증폭되었지만, 활성화된 림프절 샘플의 경우에는 어떤 유전자의 재배열도 관찰되지 않았다. 따라서 림프종의 진단에 있어서 혈액 또는 조직 도말 표본의 DNA를 이용하여 재배열된 항원 수용체 유전자의 검출을 시도한다면, 특이적이고 객관적으로 림프종을 감별진단 할 수 있을 것이며, 나아가 회고적 분석에도 유용하게 활용될 수 있을 것으로 사료된다.

Keywords

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