• IMMUNE NETWORK
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• 제15권4호
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• pp.199-205
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• 2015

T-bet is a critical transcription factor that regulates differentiation of Th1 cells from $CD4^+$ precursor cells. Since T-bet directly binds to the promoter of the IFN-${\gamma}$ gene and activates its transcription, T-bet deficiency impairs IFN-${\gamma}$ production in Th1 cells. Interestingly, T-bet-deficient Th cells also display substantially augmented the production of IL-2, a T cell growth factor. Exogenous expression of T-bet in T-bet deficient Th cells rescued the IFN-${\gamma}$ production and suppressed IL-2 expression. IFN-${\gamma}$ and IL-2 reciprocally regulate Th cell proliferation following TCR stimulation. Therefore, we examined the effect of T-bet on Th cell proliferation and found that T-bet deficiency significantly enhanced Th cell proliferation under non-skewing, Th1-skewing, and Th2-skewing conditions. By using IFN-${\gamma}$-null mice to eliminate the anti-proliferative effect of IFN-${\gamma}$, T-bet deficiency still enhanced Th cell proliferation under both Th1- and Th2-skewing conditions. Since the anti-proliferative activity of T-bet may be influenced by IL-2 suppression in Th cells, we examined whether T-bet modulates IL-2-independent cell proliferation in a non-T cell population. We demonstrated that T-bet expression induced by ecdysone treatment in human embryonic kidney (HEK) cells increased IFN-${\gamma}$ promoter activity in a dose dependent manner, and sustained T-bet expression considerably decreased cell proliferation in HEK cells. Although the molecular mechanisms underlying anti-proliferative activity of T-bet remain to be elucidated, T-bet may directly suppress cell proliferation in an IFN-${\gamma}$- or an IL-2-independent manner.

• IMMUNE NETWORK
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• 제20권1호
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• pp.5.1-5.15
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• 2020

The γδ T cells are unconventional lymphocytes that function in both innate and adaptive immune responses against various intracellular and infectious stresses. The γδ T cells can be exploited as cancer-killing effector cells since γδ TCRs recognize MHC-like molecules and growth factor receptors that are upregulated in cancer cells, and γδ T cells can differentiate into cytotoxic effector cells. However, γδ T cells may also promote tumor progression by secreting IL-17 or other cytokines. Therefore, it is essential to understand how the differentiation and homeostasis of γδ T cells are regulated and whether distinct γδ T cell subsets have different functions. Human γδ T cells are classified into Vδ2 and non-Vδ2 γδ T cells. The majority of Vδ2 γδ T cells are Vγ9δ2 T cells that recognize pyrophosphorylated isoprenoids generated by the dysregulated mevalonate pathway. In contrast, Vδ1 T cells expand from initially diverse TCR repertoire in patients with infectious diseases and cancers. The ligands of Vδ1 T cells are diverse and include the growth factor receptors such as endothelial protein C receptor. Both Vδ1 and Vδ2 γδ T cells are implicated to have immunotherapeutic potentials for cancers, but the detailed elucidation of the distinct characteristics of 2 populations will be required to enhance the immunotherapeutic potential of γδ T cells. Here, we summarize recent progress regarding cancer immunology of human γδ T cells, including their development, heterogeneity, and plasticity, the putative mechanisms underlying ligand recognition and activation, and their dual effects on tumor progression in the tumor microenvironment.

• 대한기계학회논문집A
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• 제35권11호
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• pp.1415-1422
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• 2011

운송기계구조물의 제작에 사용되는 판재 알루미늄 합금재료는 일정한 피로응력조건하에서 두께에 따라 균열진전속도의 차이를 보인다. 이러한 두께효과는 판재 알루미늄합금의 주요한 피로파괴특성 중 하나이다. 본 연구에서는 일정한 피로응력조건하에서 실시한 후판 및 박판 Al 2024-T3 합금재료의 피로 시험을 통하여 두께효과를 파악하고, 이를 형상인자인 두께비, $R_t$ 및 하중인자인 두께별 등가유효응력확대비, $U_{i}^{equ}$에 의한 상호관계식, $U_{i}^{equ}=f(R_t)$로 나타내었다. 그리고 두께효과에 의한 후판 대비 박판시험편의 균열진전 지연거동을 ${\Delta}K$ 환산법을 사용하여 정량적으로 분석하였다. 두께효과의 경향을 정량으로 나타내기 위해 두께감소율(DoT)과 응력확대계수범위, ${\Delta}K$의 감소율(DoS) 등의 값을 구하여 이들 상호관계를 규명하였다.

• 생명과학회지
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• 제33권11호
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• pp.851-858
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• 2023

Unique cartilage matrix-associated protein (UCMA)은 γ-카르복실화(Gla) 잔기가 풍부한 간외 비타민 K 의존 단백질이다. UCMA는 조골세포 분화를 촉진하고 뼈 형성을 강화한다고 보고되고 있지만 고혈당 스트레스 하에서 조골세포에 미치는 영향에 대해서는 아직 알려진 바가 없다. 본 연구에서는 고혈당 조건하에서의 MC3T3-E1 조골세포에서 UCMA 효과를 조사하기 위해 MC3T3-E1 조골세포를 높은 포도당에 노출한 후 재조합 UCMA 단백질을 처리하였다. MC3T3-E1 세포에서 활성 산소종(ROS)의 생성은 고혈당 조건하에서 증가했으나 UCMA 단백질 처리 후 감소했음을 CellROX 및 MitoSOX 염색으로 확인하였다. 또한 고혈당 조건에서 UCMA 단백질을 함께 처리한 MC3T3-E1 세포에서 정량적 중합효소 연쇄반응 결과, 항산화 유전자인 nuclear factor erythroid 2-related factor 2 와 superoxide dismutase 1 발현이 증가하였다. 동일 조건하에서 UCMA 단백질 처리에 의해 heme oxygenase-1 발현 감소와 함께 세포질에서 핵으로의 전위가 감소되었고, 미토콘드리아 분열에 관여하는 dynamin-related protein 1 발현이 증가하였으며, AKT 신호 활성은 억제되었다. 종합적으로 UCMA는 고혈당에 노출된 조골세포에서 ROS 생성을 완화하고, 항산화 유전자 발현을 증가시키고, 미토콘드리아 역학에 영향을 미치며, AKT 신호를 조절하는 것으로 보인다. 본 연구는 UCMA의 세포 메커니즘에 대한 이해를 돕고, 대사 장애 관련한 골 합병증에 대한 새로운 치료제로서의 잠재적 사용 가능성을 제시하고 있다.

• 한국과학교육학회지
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• 제33권6호
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• pp.1237-1247
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• 2013

이 연구의 목적은 이효녕 등(2013)에 의해 개발된 시스템 사고 측정 검사지의 타당도 재검 및 시스템 사고 측정 검사지를 활용하여 과학영재학생과 일반학생의 시스템 사고에 유의미한 차이가 있는지 알아보는 것이다. 과학고등학교 학생 116명과 인문계 고등학교 학생 553명에게 시스템 사고 측정 검사지를 투입하고 SPSS 18.0을 활용한 탐색적 요인분석과 Amos를 활용한 확인적 요인분석을 실시하였다. 그리고 SPSS 18.0을 활용해 과학영재학생과 일반학생 두 집단의 검사지 평균을 독립표본 t-test를 수행하여 시스템 사고에 유의미한 차이가 나타나는지 살펴보았다. 탐색적 요인분석 결과 5개의 요인구조가 나타났으며 각 요인별 4문항씩 적재되었다. 시스템 사고 측정도구의 전체 신뢰도는 .875였으며, 각 하위 요인의 신뢰도는 .693~.751로 신뢰로운 검사지로 나타났다. 확인적 요인분석에서도 ${\chi}^2/df$값이 2.765, TLI는 .907, CFI 는 .929, IFI는 .930, RMSEA는 .044로 모형 적합도 5개 수치에서 적합한 값을 보여주어 모형을 수용할 수 있다. 그리고 과학영재학생과 일반학생의 시스템 사고를 t-test를 이용해 비교해 본 결과 시스템 사고 전체 문항 뿐 아니라 모든 하위 영역에서 과학영재학생이 일반학생에 비하여 우수한 시스템 사고를 하고 있음을 보여주었다. 이 연구가 더 낳은 성과를 얻기 위해서는 학생들의 시스템 사고 향상을 살펴보는 연구에서 다른 질적 연구 도구와 함께 활용될 필요가 있으며, 나아가 다양한 학교 급에 적용 가능한 시스템 사고 측정 도구도 함께 개발될 필요가 있다.

• 복식
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• 제28권
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• pp.137-150
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• 1996

The objectives of this study were to classify daily clothing selection motives and to group men into life style types and to examine the differences in daily clothing selection motives according to the life style types. Questionnaire comprised of three sections: 36 Likert type items of life style measure; 29 Likert type items of daily clothing selection motives measure: and 4 demographic variables. Samples were 267 salary men in gheir 20's to 30's in Seoul Korea. The data were analyzed using factor analy-sis cluster analysis one-way ANOVA Dun-can's multiple range test and t-test. 1. Five factors of life style were derived by factor analysis ; F. 1 'appearance oriented' f. 2. 'positive activity' F. 3 'economy oriented': F.4 'active-leisure';F. 5 'contemporary' Four types of life style of men were defined by cluster analysis of the five factors: T. 1'posi-tive activity'; T. 2.'economy interest'; T. 3'contemporary' : T 2'economy interest' T.3'contemporary' ; T. 4 'appearance interest' 2. Four factors of daily clothing selection motives were derived by factor analysis: F.1 'clothing harmony'; F.2 'clothing utility'; F.3 'psychological influence'; F.4 'exogenous situ-ation adaptation 3. There were significant differences in daily clothing selection motives according to the life style types. The types of positive activity economy interest and contemporary had 'clothing utility' motive more than appearance interest type. Economy interest type had 'hpsychological influence' motive more than other three types. Economy interest type and appearance interest type had 'exogenous situ-ation adaptation' motive more than other two types 4. 20's and unmarried men had'psychological influence' motive more than 30's and married. Upper class had 'clothing harmony' and 'ex-ogeneous situation adaptation' motives more than middle and low. Men with less education had ' clothing utility' and 'exogenous situation adaptation' motives more than did more edu-cation.

• 태양에너지
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• 제6권2호
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• pp.3-14
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• 1986

It is desirable to collect the solar thermal energy at relatively high temperature in order to minimize the size of thermal storage system and to enlarge the scope of solar thermal energy utilization. In this study, to develop a solar collector that has both advantages of collecting solar thermal energy at high temperature and fixing conveniently the collector system for long term period, a cylindrical parabolique concentrating solar collector (M.C.P.C.S.C) was designed, which has several rows of parabolique reflectors and thin thickness such as the flat-plate solar collector, maintaining the optical form of concentrating solar collector. The thermal performance of the M.C.P.C.S.C. newly designed in this study was analysed theoretically and experimentally. The results are summarized as follows: 1) prediction equation for outlet temperature, $T_o$, of heat transfer fluid and for the thermal efficiency, ${\eta}$, of the collector were derived as; o $$T_o=[C+B1_n(\frac{I_c(t)}{pv^3})]T_i$$ o $${\eta}=\frac{A}{A_c}\dot{m}[(C-1)+B1_n(E{\cdot}di^6\frac{I_c(t)}{\dot{m}^3})]\frac{T_i}{I_c(t)}$$ 2) When the insolation on the tilted solar collector surface, $I_c$, was $900-950W/m^2$ and the heat transfer fluid was not circulated in tubular absorber, the maximum temperature on the absorber surface was $100-118^{\circ}C$, this result suggested that the heat transfer fluid could be heated up to $98-116^{\circ}C$. The maximum temperature on the absorber surface was decreased with the increase of the collector shape factor, $L_p/L_w$ 3) There was a good agreement between the experimental and theoretical value of solar collector efficiency, ${\eta}$, which was proportional to the collector shape factor, $L_p/L_w$ 4) It is desirable to continue the study on the relationship between the collector shape factor, $L_p/L_w$, and the thermal efficiency of solar collector.

• 대한병리학회지
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• 제52권6호
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• pp.386-395
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• 2018

Background: Previous studies on synchronous colorectal carcinoma (SCRC) have reported inconsistent results about its clinicopathologic and molecular features and prognostic significance. Methods: Forty-six patients with multiple advanced tumors (T2 or higher category) who did not receive neoadjuvant chemotherapy and/or radiotherapy and who are not associated with familial adenomatous polyposis were selected and 99 tumors from them were subjected to clinicopathologic and molecular analysis. Ninety-two cases of solitary colorectal carcinoma (CRC) were selected as a control considering the distributions of types of surgeries performed on patients with SCRC and T categories of individual tumors from SCRC. Results: SCRC with multiple advanced tumors was significantly associated with more frequent nodal metastasis (p=.003) and distant metastasis (p=.001) than solitary CRC. KRAS mutation, microsatellite instability, and CpG island methylator phenotype statuses were not different between SCRC and solitary CRC groups. In univariate survival analysis, overall and recurrence-free survival were significantly lower in patients with SCRC than in patients with solitary CRC, even after adjusting for the extensiveness of surgical procedure, adjuvant chemotherapy, or staging. Multivariate Cox regression analysis revealed that tumor multiplicity was an independent prognostic factor for overall survival (hazard ratio, 4.618; 95% confidence interval, 2.126 to 10.030; p<.001), but not for recurrence-free survival (p=.151). Conclusions: Findings suggested that multiplicity of advanced T category-tumors might be associated with an increased risk of nodal metastasis and a risk factor for poor survival, which raises a concern about the guideline of American Joint Committee on Cancer's tumor-node-metastasis staging that T staging of an index tumor determines T staging of SCRC.

• 대한기계학회논문집
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• 제11권1호
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• pp.132-144
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• 1987

본 논문에서는 Isida의 이론해석 방법을 참고로 하여 유한한한 폭을 갖는 두 께가 변화하는 판재에서의 응력확대계수를 2차원 이론해석한 것이다.

• Wind and Structures
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• 제27권3호
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• pp.187-197
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• 2018

In this study, aerodynamic characteristics of a horizontal axis wind turbine (HAWT) were evaluated and discussed in terms of measured data in existing onshore wind farm. Five wind turbines (T1, T2, T3, T4 and T5) were selected, and hub-height wind speed, $U_D$, wind turbine power output, P and turbine rotational speed, ${\Omega}$ data measured from these turbines were used for evaluation. In order to obtain characteristics of axial flow induction factor, a, power coefficient, $C_p$, thrust force coefficient, $C_T$, thrust force, T and tangential flow induction factor, a', Blade Element Momentum (BEM) theory was used. According to the results obtained, during a year, probability density of turbines at a rotational speed of 16.1 rpm was determined as approximately 45%. Optimum tip speed ratio was calculated to be 7.12 for most efficient wind turbine. Maximum $C_p$ was found to be 30% corresponding to this tip speed ratio.