• Korean Journal of Plant Resources
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• v.31 no.3
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• pp.195-201
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• 2018

This study was executed to evaluate the phenolic content, DPPH radical scavenging rate, and the cytotoxic effect in human cancer cell, 3T3-L1 cell from C. lanceolata extracts at various ethanol concentration. Total polyphenol and flavonoid content of the C. lanceolata at various ethanol concentration showed the high amount in 70%, 100% ethanol extract. The DPPH radical scavenging activity progressively increased in a dose-dependent manner, and showed the highest in 100% ethanol extract. The cytotoxic effect against human cancer cell of the C. lanceolata was higher in 50% and 70% ethanol extracts. In particular, the cytotoxic effect in MCF-7 cell was relatively higher than in other cells. The $IC_{50}$ (concentration causing 50% cell death) value showed the highest on MCF-7 cell ($538.39{\mu}g/m{\ell}$ in 70% ethanol extract, and exhibited significant activity against Hela cell ($637.87{\mu}g/m{\ell}$, Calu-6 cell ($728.64{\mu}g/m{\ell}$. The extract of 70% ethanol at $1,000{\mu}g/m{\ell}$ exhibited a pronounced cytotoxic effect on 3T3-L1 cell comparable to that of the other extracts, and reduced in a concentration-dependent manner.

• Journal of the Korean Chemical Society
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• v.47 no.5
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• pp.460-465
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• 2003

Structural alteration of p53 and overexpression of p53 protein are the most common genetic abnormalities in various kinds of human cancer. Mutations in the p53 tumor-suppressor gene are usually associated with an advanced development of colorectal cancer characterized by the transition from the adenoma to carcinoma stage. Mutations in exons 5-8 of the p53 gene were analyzed by the polymerase chain reaction-single strand conformation polymorphism(PCR-SSCP) and denaturing high performance liquid chromatography(DHPLC). SSCP analysis detected 7 mutations(C13109>T) in 50 colorectal cancer samples(14%) at exon 5, and DHPLC analysis detected 7 mutations (C13109>T) and 2 mutation(C13202>A, C13204>G) in 50 colorectal cancer samples(18%) at exon 5. All of 9 mutations were proved by sequencing analysis. We conclude that DHPLC is a highly sensitive and specific method for p53 gene mutations.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.31-31
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• 2017

Understanding the mechanism(s) to overcome or prevent seed ageing deterioration during storage is of fundamental interest to seed physiologists. Vitamin E (tocols) is known as a key metabolite to efficiently scavenge lipid peroxy radicals which cause membrane breakdown resulting in seed ageing. However, in rice research this hypothesis has been tested for very few lines only without considering intraspecific variation in genomic structure. Here, we present a correlation study between tocols and seed longevity using a diverse rice panel. Seeds of 20 rice accessions held in the International Rice Genebank at the International Rice Research Institute, representing aus, indica, temperate japonica and tropical japonica subpopulations, were used for tocols analysis (quantification of ${\alpha}$-, ${\beta}$-, ${\gamma}$-, ${\delta}$-tocopherol/tocotrienol by ultra performance liquid chromatography) and storage experiments at $45^{\circ}C$ and 10.9% seed moisture content (sample taken for germination testing every 3 days up to 60 days). To examine interactions between DNA sequences and phenotype, the 700k high-density single-nucleotide polymorphism marker data-set was utilized. Both seed longevity (time for viability to fall to 50%; $p_{50}$) and tocols content varied across subpopulations due to heterogeneity in the genetic architecture. Among eight types of tocol homologues, ${\alpha}$-tocopherol and ${\gamma}$-tocotrienol were significantly correlated with $p_{50}$ (negatively and positively, respectively). While temperate japonica varieties were most abundant in ${\alpha}$-tocopherol, indica varieties recorded 1.3 to 1.7-fold higher ${\gamma}$-tocotrienol than those of other subpopulations. It was highlighted that specific ratio of tocol homologues rather than total tocols content plays an important role in the seed longevity mechanism.

• Journal of the Korean Society of Radiology
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• v.10 no.2
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• pp.73-79
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• 2016

The purpose of this analysis is to compare 2D T1 FEE and 3D T1 THRIVE for demonstration of the pancreas. A total of 85(45 men, 40 women; 58 years) PACS network datum were analysis clinically indicated pancreas MRI at 1.5 T. The SNRs and CNRs of 3D T1 THRIVE(SNR: $46.42{\pm}0.67$, CNR: $28.16{\pm}0.50$) showed significantly higher values than those from 2D T1 FEE(SNR: $53.84{\pm}1.20$, CNR: $35.48{\pm}0.70$), p<0.05, The image quality of the 3D T1 THRIVE($2.63 {\pm}0.14$) was significantly superior to that with the 2D T1 FEE($2.2{\pm}0.05$), but 3D T1 THRIVE revealed several artifacts resulting in poor quality. In conclusion, The 3D T1 THRIVE technique with a 1.5 T resulting in improved SNRs, CNRs and image quality was demonstrated.

• Parasites, Hosts and Diseases
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• v.54 no.2
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• pp.139-145
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• 2016

Toxoplasmosis is a serious disease caused by Toxoplasma gondii, one of the most widespread parasites in the world. Lipid metabolism is important in the intracellular stage of T. gondii. Stearoyl-CoA desaturase (SCD), a key enzyme for the synthesis of unsaturated fatty acid is predicted to exist in T. gondii. Sterculic acid has been shown to specifically inhibit SCD activity. Here, we examined whether sterculic acid and its methyl ester analogues exhibit anti-T. gondii effects in vitro. T. gondii-infected Vero cells were disintegrated at 36 hr because of the propagation and egress of intracellular tachyzoites. All test compounds inhibited tachyzoite propagation and egress, reducing the number of ruptured Vero cells by the parasites. Sterculic acid and the methyl esters also inhibited replication of intracellular tachyzoites in HFF cells. Among the test compounds, sterculic acid showed the most potent activity against T. gondii, with an $EC_{50}$ value of $36.2{\mu}M$, compared with $EC_{50}$ values of $248-428{\mu}M$ for the methyl esters. Our study demonstrated that sterculic acid and its analogues are effective in inhibition of T. gondii growth in vitro, suggesting that these compounds or analogues targeting SCD could be effective agents for the treatment of toxoplasmosis.

• Journal of Korean Public Health Nursing
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• v.16 no.1
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• pp.135-147
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• 2002

It is essential to improve the quality of life of the elderly in aging society. Regular exercise as nursing intervention is needed to prevent diseases, maintain and promote their health, and thus promote the quality of life for the elderly, especially who generally had poor cognition for their health maintenance and promotion in a low class community. Subjects consisted of 30 women. over 65 years old in a community welfare center, who lived in the low class apartments financially supported by the local government in G city, Gangwon Province, Korea. The program consisted of dancing on music for approximately 50 minutes, 3 times a week for 10 weeks. Experimental data were analyzed with $x^2-test$, t-test, mean values, standard deviation, percentage of change and paired t-test using SAS program. After the recreation dance, the mean value of the experimental group was decreased from 7.62 to 7.44 (t=-0.258, P=0.799), and that of the control group was also decreased from 7.00 to 6.58 (t=-0.971, P=0.349) in depression. However, there were no statistically significant differences in scores of depression between two groups. In life satisfaction, the mean value of the experimental group was increased from 19.07 to 26.50 (t=2.392, P=0.030), but that of the control group was decreased from 15.92 to 13.71 (t=-1.060, P=0.305). Thus it shows that there was a statistically significant improvement on life satisfaction after the recreation dance.

• Journal of Nutrition and Health
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• v.5 no.2
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• pp.83-89
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• 1972

A survey on the nutritional state was conducted from June 11 to Nov. 30, 1971, on 673 women workers $(16{\sim}25\;yrs)$ Jorking in a factory of food industry. Total body fat content was measured by skin-fold thickness (S. F. T.) with caliper at three region of the body. And it was calculated by regression equation, with the following conclusions were abtained. 1. The mean with standard deviation body height and weight of the Korean women workers were $155.0{\pm}4.21\;cm$ and $50.1{\pm}5.31\;kg$; respectively. 2. Their mean skin-fold thickness with their standard deviation triceps region of upper arm, 14.3{\pm}3.42$; Subscapular region, $18.0{\pm}5.57mm$; Abdominal region, $19.3{\pm}4.87mm$ and total aveager, 17.2{\pm}3.91mm$. 3. The body fat contents according to body fat weight regression equation were;fat weight. 10.7 kg; fat-free weight, 39.4kg; fat weight/ fat-free weight, 27.5%; fat-free mass/ total body mass 77.6%, and fat weight/ total body mass,21.6%. 4. Since the sknin-fold thickness between the triceps and upper abdominal region and between the former and Subscapular region were correlated with the coefficients of 0.838 and 0.615 respectively. and the following regression equations were established. a) S. F. T. of Subscapular region $(mm)=0.929{\times}$S. F. T. of triceps region (mm)+4.707. b) S. F. T. of Abdominal $region(mm)=1.24{\times}$S. F. T. of triceps region(mm)+1.50. Nutritional survey on the women workers in a fact ory of food industry, performed as above by the S. F. T. method, elucidated the fact that their nutritional state is as good as the control healthy female, and that their energy uptake may well be said to be satisfactory.

• Clinics in Shoulder and Elbow
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• v.22 no.1
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• pp.3-8
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• 2019

Background: This study aimed to compare the subscapularis muscle volume between the intact groups (group I) and supraspinatus tendon tear groups (group T) based on the sex and three different age groups. Methods: Subjects with a group I and subjects with group T without any other lesions were retrospectively evaluated from among patients who received a magnetic resonance imaging (MRI) scan between January 2011 and December 2013. The MRI scans were studied by a consultant radiologist. The subscapularis muscle volume was compared according to the age and sex; the age groups were categorized as patients in their 40s, 50s, and 60s. The volume of subscapularis muscle was measured by three-dimensional reconstructed images acquired through the axial section of 1.5T MRI. Results: No statistically significant differences were observed between subscapularis muscle volume of the group I and group T, except for male patients in their 50s (group I: $100,650mm^3$ vs. group T: $106,488mm^3$) and 60s (group I: $76,347mm^3$ vs. group T: $99,549mm^3$) (p<0.05). Males had a larger mean volume of subscapularis muscle than females, and the subscapularis muscle volume decreased in a linear manner with increasing age. Conclusions: Decrease in subscapularis muscle volume was observed with increasing age, and the impact of supraspinatus tear on subscapularis muscle volume is age and sex dependent.

• Management & Information Systems Review
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• v.35 no.3
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• pp.231-247
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• 2016

This study, according to the amendment of the Act on External Audit, on the basis of the number of input auditors, the content of auditing, and audit times as additional informations, calculates the average of the samples listed companies with assets of one hundred billion KRW. However, since the actual number of the subject corporations of which asset is 100 billion KRW is small, it is difficult to extract data with reference to this asset size. Therefore, the samples were extended to 50 corporations of which asset is 10% less than 100 billion KRW and other 50 more corporations of which asset is 10% more than 100 billion KRW. As a result, a total of 100 corporations were included as samples. To calculate the average of the audit times, a t-test was performed between the two groups. The result of the t-test showed that there is not a significant difference between the two groups. According to the analytical results of the t-test, the average of the number of input auditors and the average of audit times were calculated with respect to all the 100 samples. A further analysis showed that the average of audit content in the present study was compared with the estimated values in the study of Mun (2016). Although the results of this study may not be the optimal number, they may be used as a fundamental index which may be compared with the audit times and the audit fees in the current audit market where there is not an available reference. In addition, the amendment of the Act on External Audit may enhance the independence of auditors and the transparency of accounting system when compared with the previous system where only the total audit times were disclosed.

• Journal of Power Electronics
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• v.18 no.3
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• pp.694-701
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• 2018

This paper proposes a new single-phase power converter topology for changing the frequency of AC voltage. The proposed single-phase frequency converter (SFC) includes a T-type multi-level power converter (TMPC), a frequency decoupling transformer (FDT) and a digital signal processor (DSP). The TMPC can convert a 60 Hz AC voltage to a DC voltage and then convert the DC voltage to a 50 Hz AC voltage. Therefore, the output currents of the two T-type power switch arms have 50 Hz and 60 Hz components. The FDT is used to decouple the 50 Hz and 60 Hz components. The salient feature of the proposed SFC is that only one power electronic converter stage is used since the functions of the AC-DC and DC-AC power conversions are integrated into the TMPC. Therefore, the proposed SFC can simplify both the power circuit and the control circuit. In order to verify the functions of the proposed SFC, a hardware prototype is established. Experimental results verify that the performance of the proposed SFC is as expected.