• Title/Summary/Keyword: System Production Process

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Cultural Performances of Two Escherichia coli Host- vector Systems for Production of $\beta$-Galactosidase ($\beta$-Galactosidase 생산을 위한 두 대장균 숙주-벡터의 배양 특성)

  • Choi, D.K;Park, Y.H.
    • Microbiology and Biotechnology Letters
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    • v.15 no.6
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    • pp.396-401
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    • 1987
  • Protein productivities of a cloned gene ($\beta$-galactosidase) and the cultural performances of two recombinant Escherichia coli strains, which use different host-vector systems, were studied. E. coli JM109/pTBG10 strain which carries Tac promoter had higher protein productivity than E. coli MH3000 (pRKc1857)/pASI(lacZ) strain which carries pL promoter. Induction of protein syn-thesis was optimum at the initial-and mid-logarithmic growth phases for both strains. Oxygen demand was observed to be very high during the cloned gene expression, and could be alleviated to some extent through pH control. The ratio of specific growth rates of plasmid-harboring to plasmidfree cell, $\mu$+ /$\mu$-, of the high productivity strain was observed to be lower than that of the low productivity one. Plasmid stability was analyzed for 20-30 generations, and it was found that the traction of plasmid-harboring cells dropped to l0% level in about 25 generations for both strains when the cloned gene expression was induced.

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Factors affecting the degree of Satisfaction with Workers' Working Environment engaging in Painting Process (도장 근로자의 작업 환경 만족도에 영향을 미치는 요인)

  • Lim, Nam Gu;Roh, Jaehoon;Chang, Kyu Yeob;Song, Jae Suk;Won, Jong Uk;Kim, Chi Nyon
    • Journal of Korean Society of Occupational and Environmental Hygiene
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    • v.9 no.1
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    • pp.73-86
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    • 1999
  • This study was performed to find out factors affecting degree of satisfaction with workers' working environment. The study subjects were 165 workers who were working at painting process of twelve furniture manufacturing factories in Incheon area. We used self-administered questionnaire containing general characteristics, working conditions, the degree of satisfaction with working environment, and the degree of satisfaction with working condition. In addition, we measured the exposure levels of occupational hazardous agents. The results were as follows; 1. Correlations between the degree of satisfaction with workers' working environment and the measured of occupational hazardous agents did not affect the degree of satisfaction with workers' working environment. 2. Factors affecting the degree of satisfaction with workers' working environment were not the exposure levels of occupational hazardous agents, but the degree of satisfaction with working condition, size of industry, health status, and types of ventilation system. As the degree of satisfaction with working condition was increased, the degree of satisfaction with working environment was increased. Also, for the workers who had good health status, the degree of satisfaction with work environment was appeared to be high. To increase the degree of satisfaction, with workers' working environment, firstly, effective work management and production control are required. Also, the worker's effort to negotiate continuously with top manager on improving working conditions is required. Secondly, developing heath promotion program to be appropriate for the characteristics of worksite is required. Moreover, leading worker's participation in health promotion program is very important to increase the degree of satisfaction with workers' working environment.

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Recovery of high quality external carbon sources using crystallization from pretreated excess activated sludge by alkali and ozone (알칼리-오존 동시 전처리된 잉여슬러지로부터 결정화를 이용한 고품질 외부탄 소원 회수)

  • Seo, In S.;Kim, Hong S.;Kim, Byung G.;Kim, Youn K.
    • Journal of Korean Society of Water and Wastewater
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    • v.22 no.6
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    • pp.641-646
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    • 2008
  • In this research, recovery of high quality organics from excess activated sludge and its potential as a external carbon sources for BNR process was studied. By simultaneous treatment of alkali and ozone, TSS concentration was reduced by 32%, and RBDCOD fraction was increased by 76.2%, and major constitute of produced organic were acetic acid and propionic acid. Also, nitrogen and phosphorus were greatly solubilized. However, because acid-hydrolyzable phosphorus(AHP) was major part of solubilized phosphorus, $NH_4{^+}-N$ and $PO_4{^3}-P$ concentration were insufficient for effective formation of crystal like as MAP(Magnesium Ammonium Phosphate) and hydroxyapatite. By placing BPR reactor before alkali-ozone treatment reactor, $PO_4{^3}-P$ concentration in pretreated sludge was increased by 1.8 times, and improved potential of phosphorus recovery by crystallization. In experiment of crystallization, hydroxyapatite formation was more easily applied than MAP. By hydroxyapatite formation, $SCOD/PO_4-P$ ratio was greatly increased from 32.7 at control to 141.9 at $Ca^{2+}/PO{_4}^{3-}-P$ mole ratio of 2.4. The results based on this study indicated that the proposed system configuration has potential to reduce the excess sludge production, to recover phosphorus in usable forms as well as utilize organics as a external carbon source in BNR process.

Size Tailored Nanoparticles of ZrN Prepared by Single-Step Exothermic Chemical Route

  • Lee, Sang-Ki;Park, Kyung-Tae;Ryu, Hong-Youl;Nersisyan, Hayk H.;Lee, Kap-Ho;Lee, Jong-Hyeon
    • Korean Journal of Materials Research
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    • v.22 no.5
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    • pp.243-248
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    • 2012
  • ZrN nanoparticles were prepared by an exothermic reduction of $ZrCl_4$ with $NaN_3$ in the presence of NaCl flux in a nitrogen atmosphere. Using a solid-state combustion approach, we have demonstrated that the zirconium nitride nanoparticles synthesis process can be completed in only several minutes compared with a few hours for previous synthesis approaches. The chemistry of the combustion process is not complex and is based on a metathesis reaction between $ZrCl_4$ and $NaN_3$. Because of the low melting and boiling points of the raw materials it was possible to synthesize the ZrN phase at low combustion temperatures. It was shown that the combustion temperature and the size of the particles can be readily controlled by tuning the concentration of the NaCl flux. The results show that an increase in the NaCl concentration (from 2 to 13 M) results in a temperature decrease from 1280 to $750^{\circ}C$. ZrN nanoparticles have a high surface area (50-70 $m^2/g$), narrow pore size distribution, and nano-particle size between 10 and 30 nm. The activation energy, which can be extracted from the experimental combustion temperature data, is: E = 20 kcal/mol. The method reported here is self-sustaining, rapid, and can be scaled up for a large scale production of a transition metal nitride nanoparticle system (TiN, TaN, HfN, etc.) with suitable halide salts and alkali metal azide.

A novel method for manufacturing macroscale patterns to enhance electrical efficiency by Triboelectric generator (마찰전기 발전기의 전기 효율을 향상하기 위한 macroscale 패턴 제조 방식 연구)

  • Yang, Jun-Ho;Lee, Jaeyoung
    • Journal of Energy Engineering
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    • v.29 no.1
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    • pp.75-84
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    • 2020
  • This study investigates a simple, yet effective and affordable, manufacturing method to increase the electrical efficiency by triboelectric generator (TEG) applying 3D printers. In this study, we propose the newly manufacturing method for producing a macroscale surface patterning. Overall experiments were conducted in designed test-bed chamber system which can control the magnitude and frequency of the frictional force and the relative humidity. Furthermore, we can demonstrate the voltage enhancement of macroscale surface patterns about 1.6-fold. The peak voltage producing by TEG was as high as 18 V. In comparison with conventional process that employ micro- and nanoscale patterns, the proposed process by 3D printer is faster and more suitable for mass production.

Spherical UO3 Gel Preparation Using the External Gelation Method (External Gelation 방법을 이용한 구형 UO3 Gel 입자 제조)

  • Jeong, KyungChai;Kim, YeonKu;Oh, SeungChul;Cho, Moon-Sung;Lee, YoungWoo;Chang, JongWha
    • Journal of the Korean Ceramic Society
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    • v.42 no.11 s.282
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    • pp.729-736
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    • 2005
  • HTGR (High Temperature Gas-cooled Reactor) is spotlighted to next generation nuclear power plant for producing the clean hydrogen gas and the electricity. In this study, the spherical $UO_3$ gel particles were prepared by the external gelation process, and the characteristics of these particles were analyzed the particle shape, composition of precipitate, and thermal decomposition characteristics with the Streoscope, FT-IR, and X-ray diffractometer. Raw material of the ADUN (Acid Deficient Uranyl Nitrate) solution, which has [$NO_3$]/[U] mole ratio = 1.75, was obtained from dissolution of the $U_{3}O_{8}$ powder with concentrated $HNO_3$, and its concentration is 3.5 M-U/l. The broth solution is prepared with the ADUN, urea, PVA, and THFA solution. The droplets of the broth solution was made through a nozzle system. From this study, we obtained the following results; 1) an externel chemical gelation process is a suitable method in the spherical $UO_3$ particle production, 2) the particle shape are changed by an urea mixing time, THFA volume, and the viscosity of the broth solution, 3) the amorphous $UO_3$ particles obtained from these experiments was converted to $U_{3}O_{8}$ and then $UO_2$ by heat treatment in hydrogen atmosphere at $600^{\circ}C$.

- A Study on KS A ISO 2859-2 and 2859-3 for the Best Companies in Safety management - (안전경영 우수기업을 위한 KS A ISO 2859-2 및 2859-3에 대한 고찰)

  • Lim Jae Geun;Cho Byung Sun;Jung Soo Il
    • Journal of the Korea Safety Management & Science
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    • v.6 no.4
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    • pp.83-97
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    • 2004
  • The growing importance of product quality is becoming more and more daily apparent as we enter the age of globalization around the world. And the product safety is getting more focus as well as product quality. As a result, many domestic companies are putting a lot of emphasis on safety measures and management activities and these companies' products are mainly superior to other companies' product. When we separate the product quality procedure into 3 steps, i.e. quality secure-quality confirm-quality guarantee, of course the 1st step is the most important, but also End step of product quality confirm process is important. And in mass production environment, sampling inspection is more desirable than the 100 % inspection procedure. As a part of globalization trend, KS system is also being revised and reestablished based on ISO, IEC, etc. which are based on international standard. Conventional KS sampling inspection standards were in many areas quite different from ISO sampling standards, only KS A 3102, 3104, 3151 are left and the rest become obsolete, and even the ones that are still around are planned to the gone step by step. It has been already 3 years since the new KS A ISO 2859-0-3 sampling inspection process has been established which the abolition of the popular KS A 3101, KS A 3105, KS A 3109, yet the implementation rate is very slow. This study will attempt to analyze the new KS A ISO 2859-2 and KS A ISO 2859-3, and try to understand the difference as that the new standard can be easily understood and used widely among companies, by using examples. Our attempt is to help implement with the companies with active safety involvement but the final result can be spread among other companies as well in the near future.

Development of an Injection Molded Disposable Chaotic Micromixer: Serpentine Laminating Micromixer (II) - Fabrication and Mixing Experiment - (사출 성형된 일회용 카오스 마이크로 믹서의 개발: 나선형 라미네이션 마이크로 믹서 (II) - 제작 및 혼합 실험 -)

  • Kim Dong Sung;Lee Se Hwan;Kwon Tai Hun;Ahn Chong H.
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.29 no.10 s.241
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    • pp.1298-1306
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    • 2005
  • In this paper, Part II, we realized the Serpentine Laminating Micromirer (SLM) which was proposed in the accompanying paper, Part I, by means of the injection molding process in mass production. In the SLM, the higher level of chaotic mixing can be achieved by combining two general chaotic mixing mechanisms of splitting/recombination and chaotic advection by the successive arrangement of 'F'-shape mixing units in two layers. Mold inserts for the injection molding process of the SLM were fabricated by SU-8 photolithography and nickel electroplating. The SLM was realized by injection molding of COC (cyclic olefin copolymer) with the fabricated mold inserts and thermal bonding of two injection molded COC substrates. To compare the mixing performance, a T-type micromixer was also fabricated. Mixing performances of micromixers were experimentally characterized in terms of an average mixing color intensity of a pH indicator, phenolphthalein. Experimental results show that the SLM has much better mixing performance than the I-type micromixer and chaotic mixing was successfully achieved from the SLM over the wide range of Reynolds number (Re). The chaotic micromixer, SLM proposed in this study, could be easily integrated in Micro-Total-Analysis- System , Lab-on-a-Chip and so on.

A Study on Framing Techniques of Landscape Assessment Using the Analytic Hierarchy Process - The Assessment on the Landscape Control Points - (AHP 기법을 활용한 경관평가법 작성에 관한 연구 - 경관통제점에서의 평가 -)

  • Suh Joo-Hwan;Yang Hee-Seung
    • Journal of the Korean Institute of Landscape Architecture
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    • v.32 no.4
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    • pp.94-104
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    • 2004
  • This study creates the LCP (Landscape Control Point) through the survey of spot sites for the quality of landscape assessment, which is based on an objective and departmentalized data base; the landscape assessment was achieved by production of weight value with the AHP (Analytic Hierarchy Process) technique, selection of requisites for the landscape assessment with the IVERSON method and visual area analysis with GIS (Geographic Information System). Futhermore, validity of the landscape assessment was verified by analysis of the correlation between physical amount and aesthetic amount. The purpose of this study is to suggest the basic essential data for landscape assessment and landscape planning by the characteristics of landscape based on verification of the suggested landscape assessment methods. The results of this study are summarized below. 1. In the adaptation of landscape assessment using GIS, the landscape assessment points of LCP 18, 17 and 16, which have more visible elements such as hill area, mountain area, and forest and farm land, were indicated to be higher than the others. In contrast, the landscape assessment points of LCP 13, 6 and 10, which have less visible elements, were relatively lower than the others. 2. In the visible preference measuring method, LCP 4, 14, and 16 showed high points of landscape assessment with 3.46, 3.4, and 3.18 each. With the more natural environments such as hill area, mountain area, and forest and farm land, higher results were shown. In contrast, LCP 7, 1, and 9 showed low points of landscape assessment with 2.24, 2.36, and 2.53 each. 3. In this study, a coefficient of 0.746 was gained by the analysis of correlation between the points of landscape assessment method and the points of visual preference from a slide show. This has 99 percent of probability in statistical data. 4. In conclusion, with the demonstration of the correlation between the landscape assessment method based on the AHP technique and the aesthetic amount (preference proportion), the practical use of landscape assessment can be demonstrated by the suggested landscape assessment method.

A study on the advanced method of aging manufacturing factory (노후화된 제조공장의 고도화 방법에 관한 연구)

  • Kim, Jeong-Min;Jang, Jong-Wook
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2018.10a
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    • pp.69-71
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    • 2018
  • Looking at Korea's manufacturing industry, there are many old manufacturing plants. In fact, the manufacturing process of the product inventory management and the unit price of the product are all created by using Excel, and the factory is operated by using it. Also, the operator can not predict the failure of the equipment in order to produce the product at work. Problems related to this may result in the loss of the documents during the instruction and work process between the manager and the worker, and the communication between the manager and the worker can not be properly performed, There is appear a situation in which the operation is continued by using the equipment without recognizing in the failure. In this paper, we propose a method for upgrading the aging manufacturing plant to improve the productivity and productivity of the product by predicting the efficient inventory management, unit price management, production volume, and the operator's failure prediction.

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