• 한국발생생물학회지:발생과생식
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• 제15권1호
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• pp.61-69
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• 2011

Previously, we have shown that Bcl2l10 as a member of Bcl-2 family, key regulators of the apoptotic process, is dominantly expressed in oocytes of ovary but several member of the Bcl-2 family are not expressed in oocytes. Recent our studies had been processed about roles and regulatory mechanisms of Bcl2l10 in oocytes. Microinjection of Bcl2l10 RNAi into the cytoplasm of germinal vesicle oocytes resulted in metaphase I (MI) arrest and exhibited abnormalities in their spindles and chromosome configurations (Yoon et al., 2009). The present study was conducted to elucidate the downstream genes regulated by Bcl2l10 and signaling networks in Bcl2l10 RNAi microinjected oocytes by using microarray analysis. Surprisingly, we found that a large proportion of genes regulated by Bcl2l10 RNAi were involved in the cell cycle and actin skeletal system regulation as important upstream genes of Bcl2l10. Among the transcripts with highly significant fold changes more than 2-fold, Tpx2 and Cep192 are 16.1- and 8.2-fold down regulated respectively by Bcl2l10 RNAi. Tpx2 and Cep192 are known as cofactors that control Aurora A kinase activity and localization. Therefore, we concluded that Bcl2l10 may have important roles during oocyte meiosis as functional upstream regulator of Tpx2 and Cep192.

• 유기물자원화
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• 제10권1호
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• pp.128-138
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• 2002

폐기물 재활용은 폐기물 관리에 있어서 감량화 다음으로 중요한 개념이다. 폐기물 중 특히 포장폐기물은 재활용시 환경성과 경제성을 지니고 있어 재활용이 용이하다. 그러나 그 중에서도 포장폐기물 중 점점 더 증가 추세에 있는 화장품용기는 재활용품목이 아닌 일반폐기물로 분류되어 버려지고 있다. 화장품 산업의 규모와 비례하여 나타나는 산업쓰레기의 처리문제는 심각한 환경문제를 유발하는 요인이 되고 있다. 또한 화장품은 대부분 플라스틱과 유리 등 복합재료로 만들어져 있어 재활용하기가 어렵다. 화장품용기는 분리수거 하기도 힘이 들고 분리 수거한 용기들도 재질이 복합적이기 때문에 재생이 어려운 경우가 많다. 이에 본 연구에서는 현재의 화장품 용기 재활용과 관련하여 갖고 있는 소비자들의 의식을 조사하였다. 조사결과에 대한 용기 재활용을 위한 증진방안은 다음과 같다. 첫째, 화장품 용기 관련 법 및 제도개선 분야로 화장품법 및 예치금, 부담금 제도 개선과 화장품 용기류 재활용을 위한 기술개발, 리필제품의 확대, 화장품용기만의 회수체계 확립, 생산자의 화장품 재활용 적극 참여 유도가 이루어져야 한다. 둘째, 화장품 용기 디자인 개선 분야로 용기 재질 단순화 및 규격화,화장품 라이프 사이클 확대, 재활용 가능한 재료 선택, 화장품 용기의 비용감소, 기업의 재활용 용기 개발 적극 참여가 이루어져야한다. 결론적으로 포장폐기물 중 화장품 용기의 재활용을 증진하기 위해서는 관련 제도 개선 뿐만 아니라 정부와 기업의 적극적 참여,그리고 소비자들의 적극적 재활용의식이 종합적으로 발전을 이루어야 한다. 그리고 무엇보다도 기업에서 화장품 품질 개선을 위한 투자와 개발 뿐만 아니라 화장품 용기의 환경친화적인 용기 개발을 위하여 많은 노력이 우선시 되어야 할 것이다.

• 대한간호
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• 제34권4호
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• pp.70-82
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• 1995

During the middle age of a woman's life cycle. several health changes and problems occur. Therefore. middle aged women must manage their health and maintain quality life by coping with bodily changes. However. today there is not enough research and health programs for middle aged women. Data from the study will be used for health promotion program development of middle aged women. Data was collected from January 21 to 24. 1995 by telephone interview. Four hundred middle aged women between 40 and 59 years old and living in Chon Ju City were interviewed. The results of this study were as follow: 1. Frequency rate of menopausal symptoms was 38.4%. The most serious menopausal symptom was psychosomatic symptom. Mean score orders of experienced symptoms were back pain, joint pain(1.80). nervousness(1.80). general weakness(1.67). 2. The most important problems as perceived by the clients were children(45.8%), health (24.0%) and economics(7.8%). The most serious health problems were concerning the muscle-skeletal system(45%) such as arthritis. spinal disk problems and osteoporosis. Adult diseases04.S%) such .as hypertension and diabetes were also health concerns. Health management activities reported were exercise(22.5%), social activity02%) and inactivity(53%)' 33% of clients were interested in health groups and they wanted a program of health education, exercise and social activity to be provided. 3. General characteristic variables were significantly related to the level of menopausal symptoms as follows: age(t=-2.06, p=0.040), status of marriage(t=-3.56, p=0.000), educational level (F=4.35. p=0.05) and menopausal status(t=4.37, p=0.000).

• 한국수소및신에너지학회논문집
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• 제29권4호
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• pp.339-356
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• 2018

Although fuel cell systems have advantages in terms of electric efficiency and environmental impact compared with conventional power systems, fuel cell systems have not been deployed widely due to their low reliability and high price. In order to guarantee the lifetime of 10 years, which is the commercialization goal of Polymer electrolyte fuel cells (PEFCs), it is necessary to improve durability and reliability through optimized operation and maintenance technologies. Due to the complexity of components and their degradation phenomena, it's not easy to develop and apply the diagnose and prognostic methodologies for PEFCs. The purpose of the paper is to show the current state on PEFC prognostic technology for condition based maintenance. For the prognostic of PEFCs, the model driven method, the data-driven, and the hybrid method can be applied. The methods reviewed in this paper can contribute to the development of technologies to reduce the life cycle cost of fuel cells and increase the reliability through prognostics-based health management system.

• Journal of Microbiology and Biotechnology
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• 제19권1호
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• pp.83-92
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• 2009

Individual immunochromatographic assays (ICG) for ochratoxin A (OTA) and zearalenone (ZEA) were optimized and used in the development of a one-step simultaneous immunochromatographic assay (OS-ICG) for the rapid multianalysis of two mycotoxins in corn samples. The nitrocellulose membrane of the OS-ICG was treated with OTA-bovine serum albumin (BSA), ZEA-ovalbumin (OVA), and anti-mouse IgG in the OTA test, ZEA test, and control zones, respectively. Monoclonal antibody-gold conjugates (OTA3 MAb-gold and ZEA2C5 MAb-gold) were sprayed onto the conjugate pad. The visual detection limits were 2.5 and 5 ng/ml for OTA and ZEA, respectively, and the results were obtained within 15 min after starting the analysis. An efficient, simple, and rapid extraction method using 30% MeOH/PBS was established and validated by analyzing the corn samples spiked with OTA/ZEA mixtures (0/0, 5/10, 10/20, and $20/30\;{\mu}g/kg$). The cut-off values of the OS-ICG for the spiked corn were 5 and $10\;{\mu}g/kg$ for OTA and ZEA, respectively. Natural corn samples were analyzed by OS-ICG, direct competitive enzyme-linked immunosorbent assay (DC-ELISA), and HPLC. Results of the OS-ICG were in good agreement with those obtained by DC-ELISA and HPLC. The developed OS-ICG offers a rapid, easy-to-use, and portable analytical system and can be used as a convenient qualitative tool for the on-site simultaneous determination of OTA and ZEA in cereals, food, and agricultural products in one analytical cycle.

• 한국신뢰성학회지:신뢰성응용연구
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• 제17권4호
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• pp.280-288
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• 2017

Purpose: PBA buried in underwater requires high reliability because of its mission critical characteristic and harsh operational environment during its life cycle. Therefore, various reliability improvement activities are necessary. The defect on PBA manufacturing process have been studied, as a result, many activities and standards have been presented. However, there are less studies regarding failure pattern on physical features based on design. In this paper, we studied a possible failure patten based on physical features that is related with manufacturing process of PBA. And reliability improvement design based on PoF (Physical of Failure) were intruduced in this paper. Methods: A reliability prediction simulation were performed on the components A and B of the H system using Sherlock Software which is a PoF commercial tool from DFR solution. Solder fatigue and PTH fatigue analysis based on thermal cycling profiles and random vibration was analyzed on three earthquake response spectrum. Result: It was validated that life time and reliability improvement design through solder fatigue and PTH fatigue analysis in case of component. For compoenet B, random vibration fatigue was additionally analyzed and validated reliability for earthquakes profile. Conclusion: In design stage prior to manufacturing, PoF can be analyzed, and it is possible to make a reliability improvement/validated design using design data. This study can be applied in every design step and contribute to make more stable development product.

• 산업경영시스템학회지
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• 제39권3호
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• pp.170-179
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• 2016

In this paper we have tackled the outstanding inventory planning problems over new product launching period in a more holistic manner by addressing first the definition of efficient business rules to effectively control and reduce the inventory risks followed by the rigorous explanations on the implementation guide on suggested inventory planning rules. It is not unusual for many companies in the consumer electronics market to make a great effort to reduce the time to launch a new product because the ability to bring out higher performing products in such a short time period greatly increases the probability for them to remain competitive in the high tech market. Among so many newly developed products, those products with new features and technologies appeal to many potential customers while products which fail to win customers by design and prices rapidly disappear in the market. To adapt to this business environment, those companies have been trying to find the answer to minimize the inventory of old products so they can move to next generation products quickly with less obsolete material. In the experimental implementation of our rule-based inventory planning, Company 'S' reduced the inventory cost for the outgoing products as low as 49% of its peak level of its preceding product version in just 5 month after the adoption of rule-based inventory planning process and system. This paper concluded the subject with a suggestion that the best performance of rule-based inventory planning is guaranteed not from one-time campaign of process improvement along with system development but the decision maker's continuing support and attention even without seeing any upcoming business crisis.

• 한국정보과학회논문지:소프트웨어및응용
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• 제31권8호
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• pp.1043-1053
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• 2004

컴포넌트 기반 소프트웨어에서는 사용될 컴포넌트의 성능이 개발되는 소프트웨어의 품질 확보에 있어서 매우 중요한 요소이다. 컴포넌트 성능에는 흔히 컴포넌트 모델이나 미들웨어에 종속적인 성능측정 도구가 이용된다. 이러한 성능측정 방법에서는 소프트웨어의 개발환경이 변경될 경우 측정 도구도 함께 수정되어야 한다는 문제점을 가지고 있다. 또한, 여러 가지 다른 모델의 컴포넌트들을 한 시스템으로 통합하는 경우에도 유사한 어려움이 존재한다. 본 논문은 이러한 문제에 대한 해결 방법으로 컴포넌트 모델이나 미들웨어에 독립적인 성능측정 방법을 제안한다. 제안된 방법은 미들웨어에 공통적으로 적용 가능한 성능측정기 모델에서 특정 미들웨어를 위한 성능측정기 모델로의 모델변환 과정을 통해 성능데이타간의 상호운용성을 보장한다. 이 방법을 이용하면 동일한 컴포넌트 모델에 따른 컴포넌트들뿐만 아니라 서로 다른 컴포넌트 모델을 기반으로 하는 컴포넌트들에 대한 성능측정이 가능하다.

• 한국정보통신학회:학술대회논문집
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• 한국해양정보통신학회 2000년도 추계종합학술대회
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• pp.67-73
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• 2000

국제 표준화 기구인 ITU, ISO/IEC/JTCl를 비롯해 지역 표준화기구인 ETSI, 국가기구인 committee T1, 한국정보통신기술협회 (TTA) 둥은 표준의 신속한 제정 및 적기의 공급을 위해, 각각의 표준화 절차에 기반한 전자적 문서처리(EDH: Electronic Document Handling) 시스템을 구축하고, 이를 통해 표준화 진행에 관련된 각종 정보를 효율적으로 처리할 수 있는 전자적 환경을 구축하는데 많은 노력을 기울이고 있다. 본 논문에서는 국제기구인 ITU-T, JTCl, 유럽의 ETSI, 미국의 Committee T1 그리고 지난해 7월 조직개편을 단행한 한국정보통신기술협회를 대상으로, 최근 인터넷관련 각종 정보처리기술을 이용하여, 이를 표준화 절차에 활용하고 있는 전자적 작업방법 및 이와 관련된 지침을 조사 ·분석하였다. 또한, 향후 전개될 EDH방법에 대해 논하고 한국의 대응방향 및 차세대 표준정보유통망의 운용구조를 제시한다.

• 한국전산구조공학회논문집
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• 제30권3호
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• pp.223-230
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• 2017

토목시설물 건설사업에 BIM(building information modeling)이 적극적으로 도입되면서 시설물의 생애주기 동안의 활용도를 극대화할 수 있는 방안이 모색되고 있으며, 그 중, 시설물 공사 중 건설장비의 동적 거동을 반영한 검토 사례는 드물고 관련 기술 수준도 낮은 상황이다. 본 연구에서는 BIM 기반으로 구성된 가상현장 내에서 동적인 가상건설 장비 모델을 이용하여 공사단계에서 발생하는 장비운용의 안전성과 효율성을 검토할 수 있는 시뮬레이션 시스템을 개발하고 이를 실제 토목 현장에 적용하여 그 효용성을 분석하였다. 개발된 시뮬레이션 시스템이 건설장비에 대한 효과적인 3D 라이브러리 구축을 통해 공사관리자가 최적의 장비를 선정하고 경제적인 공사를 실현하기 위한 계획수립을 지원할 뿐 아니라 안전한 공사가 수행되도록 다각도의 분석을 가능케 해준다는 것을 확인할 수 있었다.