• Journal of Periodontal and Implant Science
• /
• v.25 no.3
• /
• pp.720-732
• /
• 1995

The use of transforming growth $factor-{\beta}1$ which functions as a potent biologic mediator regulating numerous activities of wound healing has been suggested for the promotion of periodontal regeneration. The mitogenic effects of transforming growth $factor-{\beta}1$ on human periodontal ligament cells and human gingival fibroblasts were evaluated by determining the incorporation of $[^3H]-thymidine$ into DNA of the cells dose-dependently. Cells were prepared with primary cultured fibroblasts and periodontal ligament cells from humans, and used in experiments were the fourth or sixth subpassage. Cells were seeded with serum free Dulbecco's modified Eagle medium containing 0.1% bovine serum albumine. The added concentrations of transforming growth $factor-{\beta}1$ were 0.25, 0.5, 1, 2.5, 5ng/ml and transforming growth $factor-{\beta}1$ were added to the quiescent cells for 24hours, 48hours, 72hours. They were labeled with lnCi/ml $[^3H]$ thymidine for the last 24hour of the each culture. The results were presented as the mean counts per minute (CPM) per well and S.D. of four determinations. The results were as follows. : The DNA synthetic activity of human gingival fibroblasts was increased dose-dependently by transforming growth $factor-{\beta}1$ at 24 hours, 48 hours and 72 hours. The maximum mitogenic effects were at the 48 hour application of transforming growth $factor-{\beta}1$. The DNA synthetic activity was generally more decreased at the 72 hour application than at the 48 hour the application of transforming growth $factor-{\beta}1$. The DNA synthetic activity of human periodontal ligament cells was increased dose-dependently by transforming growth $factor-{\beta}1$ at 24 hours and 48 hours. But the DNA synthetic activity was decreased at 5ng/ml of the 72 hour application. The maximum mitogenic effects were also at the 48 hour application of transforming growth $factor-{\beta}1$. The DNA synthetic activity of human periodontal ligament cells was generally more decreased at the 72 hour application than at the 48 hour application of transforming growth $factor-{\beta}1$. In the comparision of DNA synthetic activity between the human gingival fibroblasts and human periodontal ligament cells, the human gingival fibroblasts had more activity than the human periodontal ligament cells at all time application with the concentration of transforming growth $factor-{\beta}1$. In conclusion, transforming growth $factor-{\beta}1$ has an important roles in the stimulation of DNA synthesis in human periodontal ligament cells and human gingival fibroblasts, which means an increase in collagen synthesizing cells and thus, may be useful for clinical application in periodontal regenerative procedures.

• Magazine of the Korea Concrete Institute
• /
• v.18 no.1 s.90
• /
• pp.36-43
• /
• 2006

• Journal of the Korean Graphic Arts Communication Society
• /
• v.12 no.1
• /
• pp.47-65
• /
• 1994

Synthetic photoresist have partially displaced the natural materials, but bichromated casein have remained in use until recently at etching industry such as chemical milling. The object of this paper is that the mutual relations among factors and suitable latitude in pattern formation or etching process when is to be applied the casein ammonium bichromate photoresist.

• Polymer Science and Technology
• /
• v.23 no.3
• /
• pp.282-288
• /
• 2012

• Proceedings of the PSK Conference
• /
• 2002.04a
• /
• pp.55-56
• /
• 2002

• Bulletin of the Korean Chemical Society
• /
• v.35 no.4
• /
• pp.985-986
• /
• 2014

• Proceedings of the Petrological Society of Korea Conference
• /
• 2009.05a
• /
• pp.35-35
• /
• 2009

• KSBB Journal
• /
• v.15 no.5
• /
• pp.415-422
• /
• 2000

Enzymatic resolution is becoming increasingly important in the production of optically active pharmaceutical drugs and is now challenging the traditional synthetic methods for production of a variety of chiral intermediates and products. This article reviews the recent advances in chirotechnology using enzymes as a catalyst to resolve chiral compounds. The review focuses on the recent trends in chirotechnology and the application of enzymes to the production of industrially valuable pharmaceutical drugs.

• Proceedings of the Korean Society of Dyers and Finishers Conference
• /
• 2007.11a
• /
• pp.73-74
• /
• 2007

• The Journal of Korean Institute of Electromagnetic Engineering and Science
• /
• v.20 no.2
• /
• pp.183-192
• /
• 2009

SAR(Synthetic Aperture Radar) is an all-weather imaging radar and is widely used in military and civil application. Especially high-resolution SAR images are very important in military purpose because it can be used at target recognition application. LIG Nex1 developed a SAR system called NexSAR with bandwidth of 600 MHz and resolution of 30 cm to obtain technologies required for high-resolution SAR. To achieve 600 MHz bandwidth of waveform generator, two DDSs are used and its output signals are SSB modulated. And deramp technique is used to reduce the sampling rate of ADC at high resolution mode. NexSAR has stripmap and spotlight modes and its functionality and performances are evaluated through ground and flight tests.