• Bulletin of the Korean Chemical Society
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• v.32 no.5
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• pp.1645-1649
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• 2011

[ ${\beta}$ ]Ketoacyl acyl carrier protein synthase I (KAS I) is involved in the elongation of unsaturated fatty acids in bacterial fatty acid synthesis and a therapeutic target of designing novel antibiotics. In this study, we performed receptor-oriented pharmacophore-based in silico screening of E. coli KAS I (ecKAS I) with the aim of identifying novel inhibitors. We determined one pharmacophore map and selected 8 compounds as candidates ecKAS I inhibitors. We discovered one antimicrobial compound, YKAe1008, N-(3-pyridinyl) hexanamide, displaying minimal inhibitory concentration (MIC) values in the range of 128-256 ${\mu}g/mL$ against MRSA and VREF. YKAe1008 was subsequently assessed for binding to ecKAS I using saturation-transfer difference NMR spectroscopy. Further optimization of this compound will be carried out to improve its antimicrobial activity and membrane permeability against bacterial cell membrane.

• Journal of the Korean Chemical Society
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• v.55 no.5
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• pp.830-834
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• 2011

This paper is on the industrial synthesis process of anti-oxidant RD ((2,2,4-trimethyl-1,2-dihydroquinoline polymer $(C_{12}H_{15}N)_n$. n=2-4)).The content of dimer, trimer and tetramer of RD as the inspection targets, using the orthogonal design method - take the ratios of keto-amine, the reaction time, the reaction temperatures and the ratios of catalyst acid-amine as inspect factors - to optimized the reaction condition. The results indicate that the best ratio of keto-amine is 2:1, the time of salification and condensation is 3 hours and 7.5 hours. The range of temperature of salification and condensation is $135^{\circ}C$ and $120-125^{\circ}C$, and that the best ratio of acid-amine is 0.2: 1 (the proportion is the concentration ratio for mole). Under the optimization conditions, the yield of RD was stabilized and content of RD more than 45%.

• Journal of Applied Biological Chemistry
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• v.58 no.1
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• pp.25-29
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• 2015

The determination of the optimum conditions for the conversion of ergosterol to vitamin $D_2$ in shitake mushrooms (Lentinula edodes) was studied using response surface methodology (RSM). The effects of the three main variables ambient temperature ($20-40^{\circ}C$), exposure time (60-180 min), and irradiation intensity ($0.6-1.8W/m^2$) were investigated. According to the RSM ridge analysis, the optimum conditions were as follows: ambient temperature of $34.2^{\circ}C$, exposure time of 175.6 min, and irradiation intensity of $1.41W/m^2$. Under these optimum conditions, the maximum vitamin $D_2$ content of $117.93{\mu}g/g$ in shitake mushrooms was obtained, which agreed fairly well with the predicted value of $122.60{\mu}g/g$.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.10 no.9
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• pp.4405-4418
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• 2016

Automatic image completion techniques have difficulty processing images in which the target region has multiple planes or is non-facade. Here, we propose a new image completion method that uses belief propagation based on planar priorities. We first calculate planar information, which includes planar projection parameters, plane segments, and repetitive regularity extractions within the plane. Next, we convert this planar information into planar guide knowledge using the prior probabilities of patch transforms and offsets. Using the energy of the discrete Markov Random Field (MRF), we then define an objective function for image completion that uses the planar guide knowledge. Finally, in order to effectively optimize the MRF, we propose a new optimization scheme, termed Planar Priority-belief propagation that includes message-scheduling-based planar priority and dynamic label cropping. The results of experiment show that our approach exhibits advanced performance compared with existing approaches.

• Journal of Power Electronics
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• v.18 no.5
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• pp.1523-1535
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• 2018

High-performance Phase-Locked Loops (PLLs) are critical for grid synchronization in grid-tied power electronic applications. In this paper, a new single-phase All Digital Phase-Locked Loop (ADPLL) is proposed. It features fast transient response and good robustness under distorted grid conditions. It is designed for Field Programmable Gate Array (FPGA) implementation. As a result, a high sampling frequency of 1MHz can be obtained. In addition, a new OSG is adopted to track the power frequency, improve the harmonic rejection and remove the dc offset. Unlike previous methods, it avoids extra feedback loop, which results in an enlarged system bandwidth, enhanced stability and improved dynamic performance. In this case, a new parameter optimization method with consideration of loop delay is employed to achieve a fast dynamic response and guarantee accuracy. The Phase Detector (PD) and Voltage Controlled Oscillator (VCO) are realized by a Coordinate Rotation Digital Computer (CORDIC) algorithm and a Direct Digital Synthesis (DDS) block, respectively. The whole PLL system is finally produced on a FPGA. A theoretical analysis and experiments under various distorted grid conditions, including voltage sag, phase jump, frequency step, harmonics distortion, dc offset and combined disturbances, are also presented to verify the fast dynamic response and good robustness of the ADPLL.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.13 no.11
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• pp.5512-5526
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• 2019

In sports video games, especially ball games, motion capture techniques are used to reproduce the ball-driven performances. The amount of motion data needed to create different situations in which athletes exchange balls is bound to increase exponentially with resolution. This paper proposes how avatars in virtual worlds can not only imitate professional athletes in ball games, but also create and edit their actions effectively. First, various ball-handling movements are recorded using motion sensors. We do not really have to control an actual ball; imitating the motions is enough. Next, motion is created by specifying what to pass the ball through, and then making motion to handle the ball in front of the motion sensor. The ball's occupant then passes the ball to the user-specified target through a motion that imitates the user's, and the process is repeated. The method proposed can be used as a convenient user interface for motion based games for players who handle balls.

• Journal of Microbiology and Biotechnology
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• v.26 no.4
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• pp.717-724
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• 2016

Methane (CH₄) is the most abundant component in natural gas. To reduce its harmful environmental effect as a greenhouse gas, CH₄ can be utilized as a low-cost feed for the synthesis of methanol by methanotrophs. In this study, several methanotrophs were examined for their ability to produce methanol from CH₄; including Methylocella silvestris, Methylocystis bryophila, Methyloferula stellata, and Methylomonas methanica. Among these methanotrophs, M. bryophila exhibited the highest methanol production. The optimum process parameters aided in significant enhancement of methanol production up to 4.63 mM. Maximum methanol production was observed at pH 6.8, 30℃, 175 rpm, 100 mM phosphate buffer, 50 mM MgCl₂ as a methanol dehydrogenase inhibitor, 50% CH₄ concentration, 24 h of incubation, and 9 mg of dry cell mass ml^-1 inoculum load, respectively. Optimization of the process parameters, screening of methanol dehydrogenase inhibitors, and supplementation with formate resulted in significant improvements in methanol production using M. bryophila. This report suggests, for the first time, the potential of using M. bryophila for industrial methanol production from CH₄.

• Journal of Microbiology and Biotechnology
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• v.30 no.10
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• pp.1574-1582
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• 2020

Flavonoids have diverse biological functions in human health. All flavonoids contain a common 2-phenyl chromone structure (C6-C3-C6) as a scaffold. Hence, in using such a scaffold, plenty of high-value-added flavonoids can be synthesized by chemical or biological catalyzation approaches. (2S)-Naringenin is one of the most commonly used flavonoid scaffolds. However, biosynthesizing (2S)-naringenin has been restricted not only by low production but also by the expensive precursors and inducers that are used. Herein, we established an induction-free system to de novo biosynthesize (2S)-naringenin in Escherichia coli. The tyrosine synthesis pathway was enhanced by overexpressing feedback inhibition-resistant genes (aroG^fbr and tyrA^fbr) and knocking out a repressor gene (tyrR). After optimizing the fermentation medium and conditions, we found that glycerol, glucose, fatty acids, potassium acetate, temperature, and initial pH are important for producing (2S)-naringenin. Using the optimum fermentation medium and conditions, our best strain, Nar-17LM1, could produce 588 mg/l (2S)-naringenin from glucose in a 5-L bioreactor, the highest titer reported to date in E. coli.

• JSTS:Journal of Semiconductor Technology and Science
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• v.17 no.4
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• pp.505-513
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• 2017

As mobile systems are performing various functionality in the IoT (Internet of Things) era, network-on-chip (NoC) plays a pivotal role to support communication between the tens and in the future potentially hundreds of interacting modules in system-on-chips (SoCs). Owing to intensive research efforts more than a decade, NoCs are now widely adopted in various SoC designs. Especially, studies on application-specific NoCs (ASNoCs) that consider the heterogeneous nature of modern SoCs contribute a significant share to use of NoCs in actual SoCs, i.e., ASNoC connects non-uniform processing units, memory, and other intellectual properties (IPs) using flexible router positions and communication paths. Although it is not difficult to find the prior works on ASNoC synthesis and optimization, little research has addressed the issues how to convert different protocols and data widths to make a NoC compatible with various IPs. Thus, in this paper, we address important issues on ASNoC implementation to support and convert multiple interfaces. Based on the in-depth discussions, we finally introduce our FPGA-proven full-custom ASNoC.

• Bulletin of the Korean Chemical Society
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• v.32 no.9
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• pp.3219-3222
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• 2011

Three types of ${\beta}$-ketoacyl acyl carrier protein synthase (KAS) are important for overcoming the bacterial resistance problem. Recently, we reported the discovery of a antimicrobial flavonoid, YKAF01 (3,6-dihydroxyflavone), which exhibits antibacterial activity against Gram-positive bacteria through inhibition of ${\beta}$-ketoacyl acyl carrier protein synthase III (KAS III). In this report, we suggested that YKAF01 can be an inhibitor ${\beta}$-ketoacyl acyl carrier protein synthase I (KAS I) with dual inhibitory activity for KAS I as well as KAS III. KAS I is related to the elongation of unsaturated fatty acids in bacterial fatty acid synthesis and can be a good therapeutic target of designing novel antibiotics. We performed docking study of Escherichia coli KAS I (ecKAS I) and YKAF01, and determined their binding model. YKAF01 binds to KAS I with high binding affinity ($2.12{\times}10^6$) and exhibited an antimicrobial activity against the multidrug-resistant E. coli with minimal inhibitory concentration (MIC) value of 512 ${\mu}g$/mL. Further optimization of this compound will be carried out to improve its antimicrobial activity and membrane permeability against bacterial cell membrane.