• The Plant Pathology Journal
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• 제15권1호
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• pp.38-43
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• 1999

Mutants of the monopartite geminivirus beet curly top virus (BCTV) have been screened for infectivity, systemic movement, replication and symptom development in Arabidopsis thaliana. As known by coding for coat protein, R1 mutant was not infectious and did not move systemically. R2, R3 and L2/L3 mutants produced milder symptoms compared to wild type BCTV but the infectivity was reduced by 40% to 60%. R2 ORF is thought to be involved in the regulation of ssDNA and dsDNA accumulation because only dsDNA was accumulated on R2-infected organs. Disruption of ORF L4 resulted in reduced infections, but the viral DNA was accumulated in infected organs from roots to shoot tips as much as wild type BCTV on Sei-O. In addition, 4 mutants did not produce callus-like tissues on infected organs, suggesting that L4 ORF may play a role in the induction of host cell divisions by virus infection. This result was supported by the patterns of mRNA expression and promoter analysis of the cell cycle marker gene, cycl, on Arabidopsis. cycl mRNA was accumulated on symptomatic organs by wild type BCTV infections but not by L4 mutant. We conclude that the BCTV L4 ORF is essential for symptom developments, specially callus-like formation on infected organs.

• Perspectives in Nursing Science
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• 제10권2호
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• pp.87-96
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• 2013

Purpose: The purpose of this study was to investigate the relationships of activity status, anxiety, depression, social support, symptom experience, and functional status in patients with lung cancer based on the theory of unpleasant symptoms. Methods: The participants for this study were 101 lung cancer patients who visited the out-patient department for treatment or follow-up at one hospital in Seoul. Data were collected from January 1 to February 8, 2013 using self-reported questionnaires and clinical records. To measure variables, the functional scale and symptom scale of the European Organization for Research and Treatment of Cancer (EORTC) Quality of Life Questionnaire-Core 30, EORTC QLQ-Lung Cancer 13, Eastern Cooperative Oncology Group Performance Status, Hospital Anxiety and Depression Scale, and Multidimensional Scale of Perceived Social Support were used in this study. The data were analyzed using SPSS 19.0 software for Windows. Results: The symptom experience showed more severity in patients with lower activity status, higher anxiety and depression. With lower activity status and social support, functional status was lower. When anxiety, depression, and symptom experience were higher, functional status was also lower. The significant factors predicting symptom experience were depression, anxiety, activity status, and social support, which explained 57.8% of the variance. Conclusion: These results suggest that psychological factors such as anxiety and depression had a negative influence on the symptom experience of lung cancer patients. Therefore, providing emotional support based on the patients' needs prior to providing symptom management could be a useful strategy for improving symptom experience and functional status.

• 한국콘텐츠학회논문지
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• 제9권5호
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• pp.24-32
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• 2009

우리 사회는 급속히 고령화되고 있으며 소득수준은 점점 향상되어 가고 있다. IT 기반 융합기술의 발전에 따라 u-헬스케어 서비스를 위한 인프라스트럭처가 구축되면서, 민간요법으로 알려진 수지침 처방의 중요성이 부각되고 있다. 본 논문에서는 u-헬스케어에서 상황에 따른 자가진단을 이용한 수지침 처방을 제안하였다. 제안된 방법에서는 사용자의 상황과 환경을 정의하였고 협력적 필터링을 이용하여 자가진단에 따른 적합한 수지침 처방 서비스를 예측하였다. 사용자는 제안된 시스템에 단지 병명의 입력만으로도 그에 대한 자가진단으로 정확한 수지침 처방을 얻을 수 있게 된다. 이를 GUI로 구축하여 논리적 타당성과 유효성을 검증하기 위해 실험적인 적용을 시도하였다. 따라서 상황정보 및 자가진단을 제공하여 수지침 처방에 대한 만족도와 서비스의 질을 향상시켰다.

• 한국식물병리학회지
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• 제14권5호
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• pp.507-512
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• 1998

Molecular analysis has been done for characterization of the interactions between three beet curly top virus (BCTV) strains and two Arabidopsis ecotypes in terms of virus inducible disease symptoms and infectivities. The total DNA was isolated from three tissues (shoot tips, infection origins and roots) of virus infected plants and this DNA was analyzed by quantitatively and qualitatively to elucidate virus movement and symptom development. CTV-Worland infected Col-O and Sei-O showed only symptom shown in hypersusceptible ecotype Sei-O by BCTV-worland was shoot tip stunting. Kinetics of virus DNA accumulation of three different viruses indicated that roots contained more virus DNA than shoot tips or infection origins, and that disease symptom severity was strongly correlated with virus DNA accumulation. These results suggest that the mild and Worland-specific symptoms shown in Sei-O by BCTV-worland are caused by the interactions of host factors provided by hypersusceptible ecotype and viral factors of mild strain.

• 한국임상수의학회지
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• 제34권5호
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• pp.347-352
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• 2017

Pain, an adaptive but unpleasant sensation, is the most common symptom of numerous diseases in humans and animals. Although animal patients express this symptom frequently, a lack of communication abilities hinders its recognition by veterinary physicians, thereby leading to unsatisfactory management of the symptom. On the other hand, pain itself has its own neurological mechanisms, regardless of the disease that causes it. Thus, a physician may need to know the mechanisms underlying pain development in order to properly manage the symptom in a particular disease. In this review, we attempt to provide a brief introduction to the anatomical, physiological, and neurological basis of pain transmission and sensation. Although most knowledge about these mechanisms comes from studies in humans and laboratory animals, it is generally applicable to pet, farm, or zoo animals. In addition, we summarize pain behavior in several pet, farm, and laboratory animals for its proper identification. This information will help to identify and manage pain, and thus improve welfare, in animals.

• International Journal of Industrial Entomology and Biomaterials
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• 제12권1호
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• pp.21-28
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• 2006

Peroxidase activity was measured in brown leaf spot pathogen (Myrothecium roridum) inoculated potted mulberry (Morus alba) during pre-symptomatic to various symptom development stages and compared with corresponding healthy leaf tissues. The enzyme showed a pH optimum of 7.0 and the activity was linearly increased up to 15 min of incubation. The peroxidase had a broad substrate specificity and the rates of oxidation were in the rank of pyrogallol> guaiacol> ascorbate at pH 7.0. Catechol at 10 mM inhibited 89% of guaiacol-peroxidase and 76% pyrogallol-peroxidase activities, indicated higher non-specific peroxidation in pyrogallol dependent assay system in mulberry than guaiacol. The optimum requirement for the guaiacol dependent assay was 0.2 ml (${\approx}40-60{\mu}g$ equivalent of protein) of crude enzyme source. Excepting the 8th leaf from the apex, the peroxidase activity did not vary appreciably in different leaf positions. In pre-symptomatic phases, an initial (1 to 5 min) rise of peroxidase activity was noticed in inoculated leaves, and then maintained a plateau up to 300 min. In contrary, non-infected tissue showed a slightly increased trend of enzyme level up to 420 min. In infected tissue, a sharp transient increase (3.1 fold) of peroxidase activity appeared between 300 - 420 min post infections. Afterwards, significantly different but steady maintenance of enzyme levels were observed in two treatments. On the other hand, during symptom development, a sharp increase in peroxidase activity was noticed up to 4th grade of lesion appearance (25.1 % to 50% of leaf area infection), and then declined slightly. However, in non-infected but same age healthy leaves, such huge fluctuations of enzyme level did not apparent. A high positive correlation $(R^2=0.92)$ between peroxidase activity and leaf spot development grades was also marked. The result implies that pre-symptomatic burst (between 1 - 5 and 300 - 420 min) and subsequent increased trend of guaiacol peroxidase activity may require for the symptomatic manifestation of Myrothecium leaf spot in mulberry.

• 식물병연구
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• 제28권1호
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• pp.54-56
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• 2022

2021년 7월, 고창 땅콩 재배포장에서 시들음 증상을 보이는 땅콩 지상부를 발견하였다. 병징은 잎이 갈색으로 시들어 말라 죽은 것처럼 보였으며, 채집한 식물체의 지제부를 잘라 표면소독 후 멸균수에 넣었을 때 ooze 현상을 관찰하였다. 땅콩에서 순수분리된 병원균은 16s rRNA 유전자 염기서열과 phylotype 분류, 유연관계 분석을 통해 분리된 균주가 Ralstonia pseudosolanacearum이라는 것을 확인하였다. 현재까지 국내에 보고된 풋마름병은 고추, 토마토, 감자 등을 기주로 발생한다고 알려져 있다. 본 연구는 국내 처음으로 R. pseudosolanacearum에 의해 발생한 땅콩 풋마름병을 보고하고자 한다.

• 정보과학회 컴퓨팅의 실제 논문지
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• 제22권2호
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• pp.77-82
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• 2016

증상 진단 시스템이라 함은 환자가 자신의 증상을 설명하고, 한의사가 증상에 맞는 질병 진단을 내리는 것을 말한다. 증상 진단 시스템을 자동화하기 위해서는 환자의 자연어로 이루어진 증상 설명에 대한 분석이 필요하다. 이에 본 논문에서는 증상 설명에 대하여 형태소 분석을 수행하고 한의학 병증 데이터와 비교하여 적합한 진단을 내리도록 증상 진단 시스템을 개발하였다. 증상 진단 검색의 효율을 높이기 위해서 Document형 NoSQL인 MongoDB를 이용하여 각각의 병증 데이터를 하나의 도큐먼트로 하고, 그 안의 필드값을 유연하게 관리할 수 있도록 데이터베이스를 구축하였다. 또한, 진단의 근거가 되는 한의사의 병증 설명과 환자의 증상 설명에서 사용되는 용어의 차이를 줄일 수 있도록 환자의 증상 설명을 축적하고 정제하여 일반인에게 친숙한 단어로 구성된 설명데이터를 제공할 수 있게 하였다.

• 한국콘텐츠학회논문지
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• 제16권4호
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• pp.45-55
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• 2016

본 연구는 여자 청소년들의 휴대전화 사용행태와 컴퓨터단말기증후군 증상경험을 파악하기 위한 서술적 조사연구이다. 자료 수집은 2013년 5월 6일부터 6월 3일까지 중학교 및 고등학교에 재학 중인 여학생들을 대상으로 VDT 증후군 자각증상 조사표를 이용하였다. 수집된 자료는 SPSS 21.0 프로그램을 이용하여 기술통계 및 Kruskal-Wallis test, Mann-Whitney U test 등으로 분석하였다. 연구결과 대상자들의 96.3%가 휴대전화를 사용하고 있었으며 컴퓨터단말기증후군 증상을 하나 이상 경험한 비율은 87.7%였으며, 증상경험 점수는 $.39{\pm}.41$점이었다. 눈 관련 증상을 가장 많이 경험하였으며, 근골격계 증상의 정도가 가장 심하였다. 컴퓨터단말기증후군 증상경험은 휴대전화 사용자세, 사용시간, 사용 중 휴식여부에 따라 차이가 있었다. 따라서 휴대전화 사용 시 바른 자세로 사용하고, 사용 중에는 휴식 시간을 갖는 것이 컴퓨터단말기증후군 증상경험 완화에 도움이 될 것이다. 또한 여자 청소년들이 자신의 휴대전화 이용 상태를 객관적으로 점검할 수 있도록 휴대전화 이용 상태 진단 도구의 개발과 주기적인 점검 기회를 제공할 필요가 있으며, 휴대전화 중독 위험집단에게는 중재 프로그램 또는 예방교육이 필요한 것으로 보인다.

• 대한치과기공학회지
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• 제35권2호
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• pp.157-171
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• 2013

Purpose: This study aims to present a database for the development of a healthcare management program based on the survey and analysis of self reported psychosomatic symptoms among the current dental technology students. Methods: Subjects of our study are 480 dental technology major students enrolled in a third year college located Gyeonggi, Chungcheong, and Gangwon province. Using a random sampling, we conducted a self-report survey from August 30, 2011 to October 28, 2011 and 418 reports were collected as feedback and we put an analysis on them. Results: 1. The average physical self symptom was 20.49, which is higher than the average mental self symptom(18.54). Of the subcategories of psychosomatic self symptom, we observed multiple subjective symptoms as the highest one(37.77), and aggression as the lowest(13.77). 2. As to gender, both physical and mental self symptom were statistically significant with the scale score of(p<.001). The scale score of subcategories is as follows; multiple subjective symptoms(I, p<.001), eye and skin(B, p<.001), digestive(C, p<.001), impulsiveness(H, p<.001), lie scale(L, p<.001), mental instability(J, p<.001), depression(K, p<.001), aggression(F, p<.001), irregularity of life(G, p<.001), mouth and anal(D, p<.05), nervousness(E, p<.05). 3. As for obesity, statistical significance was shown with the scale scores of physical self symptom(p<.001), multiple subjective symptoms(I, p<.001), digestive(C, p<.001), aggression(F, p<.001), depression(K, p<.01), irregularity of life(G, p<.01), respiratory(A, p<.05), eye and skin(B, p<.05), impulsiveness(H, p<.05), mental instability(J, p<.05). The scale scores in the environmental quality and life satisfaction were shown as follows; depression(K, p<.001), lie scale(L, p<.01), and irregularity of life(G, p<.05). 4. We employed multiple regression analysis to take account of general factors affecting psychosomatic self symptoms, and drew that the explanatory power of the model was proved with the scales of physical self symptom(4.1%) and mental self-symptom(3.6%). Obesity was a factor that affects physical self symptom with the scale score of(p<.01), and environmental quality and life satisfaction(p<.01) and obesity(p<.05) affect mental self symptom. Conclusion: In this analysis we observed obesity of dental technology students can influence their psychosomatic self symptoms. In this sense, it would be reasonable to develop a healthcare management and education programs that help the students maintain a healthy weight and promote their health.