• Title/Summary/Keyword: Swarming System

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Swarming Differentiation of Vibrio vulnificus Downregulates the Expression of the vvhBA Hemolysin Gene via the LuxS Quorum-Sensing System

  • Kim Moon-Young;Park Ra-Young;Choi Mi-Hwa;Sun Hui-Yu;Kim Choon-Mee;Kim Soo-Young;Rhee Joon-Haeng;Shin Sung-Heui
    • Journal of Microbiology
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    • v.44 no.2
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    • pp.226-232
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    • 2006
  • Swarming has proven to be a good in vitro model for bacterial surface adherence and colonization, and the swarming differentiation of a bacterium has been shown to be coupled with changes in the expression of virulence factors associated with its invasiveness, particularly in the early stages of infection. In this study, we attempted to determine whether the expression of vvhA, which encodes for hemolysin/cytolysin (VvhA), is either upregulated or downregulated during the swarming differentiation of V. vulnificus. The insertional inactivation of vvhA itself exerted no detectable effect on the expression of V. vulnificus swarming motility. However, in our lacZ-fused vvhA transcriptional reporter assay, vvhA expression decreased in swarming V. vulnificus as compared to non-swarming or planktonic V. vulnificus. The reduced expression of vvhA in swarming V. vulnificus increased as a result of the deletional inactivation of luxS, a gene associated with quorum sensing. These results show that vvhA expression in swarming V. vulnificus is downregulated via the activity of the LuxS quorum-sensing system, suggesting that VvhA performs no essential role in the invasiveness of V. vulnificus via the adherence to and colonization on the body surfaces required in the early stages of the infection. However, VvhA may playa significant role in the pathophysiological deterioration occurring after swarming V. vulnificus is differentiated into planktonic V. vulnificus.

UAV Swarm Flight Control System Design Using Potential Functions and Sliding Mode Control (포텐셜 함수와 슬라이딩 모드 제어기법을 이용한 무인기 군집비행 제어기 설계)

  • Han, Ki-Hoon;Kim, You-Dan
    • Journal of the Korean Society for Aeronautical & Space Sciences
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    • v.36 no.5
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    • pp.448-454
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    • 2008
  • This paper deals with a behavior based decentralized control strategy for UAV swarming utilizing the artificial potential functions and the sliding mode control technique. Individual interactions for swarming behavior are modeled using the artificial potential functions. The motion of individual UAV is directed toward the negative gradient of the combined potential. For tracking the reference trajectory of UAV swarming, a swarming center is considered as the object of control. The sliding-mode control technique is adopted to make the proposed swarm control strategy robust with respect to the system uncertainties and the varying mission environment. Numerical simulation is performed to verify the performance of the proposed controller.

A Security Protocol for Swarming Technique in Peer-to-Peer Networks (피어 투 피어 네트워크에서 스워밍 기법을 위한 보안 프로토콜)

  • Lee, Kwan-Seob;Lee, Kwan-Sik;Lee, Jang-Ho;Han, Seung-Chul
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.15 no.9
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    • pp.1955-1964
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    • 2011
  • With fast deployment of high-speed networks and various online services, the demand for massive content distribution is also growing fast. An approach that is increasingly visible in communication research community and in industry domain is peer-to-peer (P2P) networks. The P2P swarming technique enables a content distribution system to achieve higher throughput, avoid server or network overload, and be more resilient to failure and traffic fluctuation. Moreover, as a P2P-based architecture pushed the computing and bandwidth cost toward the network edge, it allows scalability to support a large number of subscribers on a global scale, while imposing little demand for equipment on the content providers. However, the P2P swarming burdens message exchange overheads on the system. In this paper, we propose a new protocol which provides confidentiality, authentication, integrity, and access control to P2P swarming. We implemented a prototype of our protocol on Android smart phone platform. We believe our approach can be straightforwardly adapted to existing commercial P2P content distribution systems with modest modifications to current implementations.

Study on Analysis of Queen Bee Sound Patterns (여왕벌 사운드 패턴 분석에 대한 연구)

  • Kim Joon Ho;Han Wook
    • The Journal of the Convergence on Culture Technology
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    • v.9 no.5
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    • pp.867-874
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    • 2023
  • Recently, many problems are occurring in the bee ecosystem due to rapid climate change. The decline in the bee population and changes in the flowering period are having a huge impact on the harvest of bee-keepers. Since it is impossible to continuously observe the beehives in the hive with the naked eye, most people rely on knowledge based on experience about the state of the hive.Therefore, interest is focused on smart beekeeping incorporating IoT technology. In particular, with regard to swarming, which is one of the most important parts of beekeeping, we know empirically that the swarming time can be determined by the sound of the queen bee, but there is no way to systematically analyze this with data.You may think that it can be done by simply recording the sound of the queen bee and analyzing it, but it does not solve various problems such as various noise issues around the hive and the inability to continuously record.In this study, we developed a system that records queen bee sounds in a real-time cloud system and analyzes sound patterns.After receiving real-time analog sound from the hive through multiple channels and converting it to digital, a sound pattern that was continuously output in the queen bee sound frequency band was discovered. By accessing the cloud system, you can monitor sounds around the hive, temperature/humidity inside the hive, weight, and internal movement data.The system developed in this study made it possible to analyze the sound patterns of the queen bee and learn about the situation inside the hive. Through this, it will be possible to predict the swarming period of bees or provide information to control the swarming period.

An Illegal Drone Tracking Scheme Using Swarming Flight (군집 비행을 이용한 불법 드론 추적 기법)

  • Kim, Ryun-Woo;Song, Hong-Jong;Ban, Tae-Won
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.26 no.6
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    • pp.943-948
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    • 2022
  • Drones have been widely used in various fields due to the rapid development of done-related technologies, which causes various problems. The schemes which can track target drones by using signal transmitted by target drones have been investigated as a key technology for anti-drone systems to solve these problems. In this paper, we investigate an illegal drone tracking system based on swarming flight that consists of multiple small drones in order to resolve the limitations of a conventional system that consists of a single drone. In addition, we also propose a scheme with which we can adaptively adjust the separation distance between small drones in a swarm according to channel situations. We analyzed the performance of the proposed scheme in terms of success ratio and the number of movements. The proposed scheme can improve the success ratio and the number of movements by 170% and 63% respectively, compared to the conventional scheme.

Systematic Singular Association for Group Behaviors of a Swarm System (스웜 시스템의 그룹 행동을 위한 조직화된 단일 연합법)

  • Jung, Hah-Min;Kim, Dong-Hun
    • Journal of the Korean Institute of Intelligent Systems
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    • v.19 no.3
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    • pp.355-362
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    • 2009
  • In this paper, we present a framework for managing group behaviors in multi-agent swarm systems. The framework explores the benefits by dynamic associations with the proposed artificial potential functions to realize complex swarming behaviors. A key development is the introduction of a set of flocking by dynamic association (DA) algorithms that effectively deal with a host of swarming issues such as cooperation for fast migration to a target, flexible and agile formation, and inter-agent collision avoidance. In particular, the DA algorithms employ a so-called systematic singular association (SSA) rule for fast migration to a target and compact formation through inter-agent interaction. The resulting algorithms enjoy two important interrelated benefits. First, the SSA rule greatly reduces time-consuming for migration and satisfies low possibility that agents may be lost. Secondly, the SSA is advantageous for practical implementations, since it considers for agents even the case that a target is blocked by obstacles. Extensive simulation presents to illustrate the viability and effectiveness of the proposed framework.

Collision Avoidance Maneuver Design for the Multiple Indoor UAV by using AR. Drone (AR. Drone을 이용한 실내 군집비행용 충돌회피 기동 설계)

  • Cho, Dong-Hyun;Moon, Sung Tae;Jang, Jong Tai;Rew, Dong-Young
    • Journal of the Korean Society for Aeronautical & Space Sciences
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    • v.42 no.9
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    • pp.752-761
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    • 2014
  • With increasing of interest in quad-rotor which has excellent maneuverability recently, a various types of multi-rotor aircraft was developed and commercialized, and there are many kinds of leisure products to be easily operated. In these products, the AR.Drone manufactured by Parrot has an advantage that it is easily operated by user due to the its internal stabilization loop in the on-board computer. Thus it is possible to design the unmanned UAV system easily by using this AR.Drone and its inner loop for the stabilization. For this advantage, KARI(Korea Aerospace Research Institute) has been developing the indoor swarming flight system by using multiple AR.Drones. For this indoor swarming flight, it is necessary that not only the position controller for each AR.Drone, but also the collision avoidance algorithm. Therefore, in this paper, the collision avoidance controller is provided for the swarm flight by using these AR.Drones.

Analysis of Quorum Sensing-Related Phenotypes of Pseudomonas aeruginosa Clinical Isolates (녹농균 임상균주의 쿼럼 센싱 관련 표현형 분석)

  • Jung, Kyung-Ju;Choi, Yu-Sang;Ha, Chang-Wan;Shin, Jeong-Hwan;Lee, Joon-Hee
    • Korean Journal of Microbiology
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    • v.46 no.3
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    • pp.240-247
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    • 2010
  • Pseudomonas aeruginosa is a Gram (-) opportunistic human pathogen causing a wide variety of infections on lung, urinary tract, eyes, and burn wound sites and quorum sensing (QS), a cell density-sensing mechanism plays an essential role in Pseudomonas pathogenesis. In order to investigate the importance of QS in the Pseudomonas infections of Korean patients, we isolated 189 clinical strains of P. aeruginosa from the patients in Pusan Paik Hospital, Busan, South Korea. The QS signal production of these clinical isolates was measured by signal diffusion assay on solid media using reporter strains. While most clinical strains (79.4%) produced the QS signals as similar level as a wild type strain, PAO1 did, where LasR, the initial QS signal sensor-regulator was fully activated, a minority of them (4.2%) produced much less QS signals at the level to which LasR failed to respond. Similarly, while 72.5% of the clinical isolates produced QS signals enough to activate QscR, an another QS signal sensor-regulator, some few of them (9%) produced the QS signals at much lower level where QscR was not activated. For further analysis, we selected 74 clinical strains that were obtained from the patients under suspicion of Pseudomonas infection and investigated the total protease activity that is considered important for virulence. Interestingly, significant portion of them showed very low protease activity (44.6%) or no detectable protease activity (12.2%). When the biofilm-forming ability that is considered very important in chronic infection was examined, most isolates showed lower biofilm-forming activity than PAO1. Similarly, significant portion of clinical isolates showed reduced motility (reduced swarming activity in 51.4% and reduced twitching activity in 41.9%), or non-detectable motility (swarming-negative in 28.4% and twitching-negative in 28.4%). Our result showed that the clinical isolates that produced QS signals at the similar level to wild type could have significantly reduced activities in the protease production, biofilm formation, and motility, and some clinical isolates had unique patterns of motility, biofilm formation, and protease production that are not correlated to their QS activity.

Characterization of Bacillus luciferensis Strain KJ2C12 from Pepper Root, a Biocontrol Agent of Phytophthora Blight of Pepper

  • Kim, Hye-Sook;Sang, Mee-Kyung;Myung, Inn-Shik;Chun, Se-Chul;Kim, Ki-Deok
    • The Plant Pathology Journal
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    • v.25 no.1
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    • pp.62-69
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    • 2009
  • In this study, we characterized the bacterial strain KJ2C12 in relation with its biocontrol activity against Phytophthora capsici on pepper, and identified this strain using morphological, physiological, biochemical, fatty acid methyl ester, and 16S rRNA gene sequence analyses. Strain KJ2C12 significantly (P=0.05) reduced both final disease severity and areas under the disease progress curves of 5-week-old pepper plants inoculated with P. capsici compared to buffer-treated controls. As for the production of antibiotics, biofilms, biosurfactant, extracellular enzyme, HCN, and swarming activity, strain KJ2C12 produced an extracellular enzyme with protease activity, but no other productions or swarming activity. However, Escherichia coli produced weak biofilm only. Strain KJ2C12 could colonize pepper roots more effectively in a gnotobiotic system using sterile quartz sand compared to E. coli over 4 weeks after treatments. However, no bacterial populations were detected in 10 mM $MgSO_4$ buffer-treated controls. Strain KJ2C12 produced significantly higher microbial activity than the $MgSO_4$-treated control or E. coli over 4 weeks after treatments. Bacterial strain KJ2C12 was identified as Bacillus luciferensis based on morphological, physiological, and biochemical characteristics as well as FAME and 16S rRNA gene sequence analyses. In addition, these results suggested that B. luciferensis strain KJ2C12 could reduce Phytophthora blight of pepper by protecting infection courts through enhanced effective root colonization with protease production and an increase of soil microbial activity.

Partial Discharge Characteristics of the XLPE/EPDM Interface in Power Cable Joint (전력케이블 접속재 XLPE/EPDM 계면의 부분방전 특성)

  • Cho, Kyung-Soon;Hong, Jin-Woong
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.20 no.9
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    • pp.780-786
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    • 2007
  • This paper describes the influence on partial discharge characteristics of defects at the model XLPE/EPDM interfaces of power cable joints. The defects of void and copper which could inadvertently be present at the joint interface. We measured ${\Phi}-n,\;{\Phi}-q$ patterns by a computer-aided partial discharge measuring system. Several parameters i, e, maximum discharge $q_{max}$ [pC], average discharge q [pC/cycle], and average angle of discharge ${\Theta}g$ [deg] were found to depend upon the defect type varying applied voltage. As the result of time evaluation, partial discharges are small different at copper defects, but is decreasing obviously about 20 minutes at void defect. It considered that difference of magnitude of total positive discharge of Q+ [pC] and total negative discharge of Q- [pC] is SPMD(swarming pulsive micro discharges).