• 대한기계학회논문집B
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• 제36권11호
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• pp.1119-1126
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• 2012

세균의 집단 행동은 생물막의 형성에 중요한 역할을 하고 있다. 본 연구에서는 대장균(E. coli) 및 고초균(B. subtilis) 군집에서 형성된 유동성 생물막의 유체역학적 특성을 비교하고자 초기에 아가 플레이트 내에서 층을 이루다가 성장하는 군집 표면 위로 자발적으로 분포되는 200 nm의 형광입자를 가시화 하였다. 대장균 군집에서는 유동하지 않는 200 nm 크기의 형광입자를 이용하여 성장하는 세균 군집의 3차원 형상 프로파일을 측정하였다. 고초균 군집의 경계에서 나타나는 와류 패턴은 고초균이 분비하는 계면활성제 내에서 유동하는 형광입자를 추적하여 가시화하였다. 본 연구는 세균의 생리 기능을 조절하는 새로운 물리적인 요소를 밝혀내고 세균의 증식 및 군집 이동에 영향을 미치는 유동성 생물막의 효과를 파악하는 첫걸음이 될 것이다.

• The Plant Pathology Journal
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• 제25권1호
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• pp.62-69
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• 2009

In this study, we characterized the bacterial strain KJ2C12 in relation with its biocontrol activity against Phytophthora capsici on pepper, and identified this strain using morphological, physiological, biochemical, fatty acid methyl ester, and 16S rRNA gene sequence analyses. Strain KJ2C12 significantly (P=0.05) reduced both final disease severity and areas under the disease progress curves of 5-week-old pepper plants inoculated with P. capsici compared to buffer-treated controls. As for the production of antibiotics, biofilms, biosurfactant, extracellular enzyme, HCN, and swarming activity, strain KJ2C12 produced an extracellular enzyme with protease activity, but no other productions or swarming activity. However, Escherichia coli produced weak biofilm only. Strain KJ2C12 could colonize pepper roots more effectively in a gnotobiotic system using sterile quartz sand compared to E. coli over 4 weeks after treatments. However, no bacterial populations were detected in 10 mM $MgSO_4$ buffer-treated controls. Strain KJ2C12 produced significantly higher microbial activity than the $MgSO_4$-treated control or E. coli over 4 weeks after treatments. Bacterial strain KJ2C12 was identified as Bacillus luciferensis based on morphological, physiological, and biochemical characteristics as well as FAME and 16S rRNA gene sequence analyses. In addition, these results suggested that B. luciferensis strain KJ2C12 could reduce Phytophthora blight of pepper by protecting infection courts through enhanced effective root colonization with protease production and an increase of soil microbial activity.

• 한국지능시스템학회논문지
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• 제19권3호
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• pp.355-362
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• 2009

본 논문은 다수의 에이전트가 있는 스웜 시스템에서 효과적인 그룹행동을 다루는 연구를 한 내용이다. 많은 에이전트들이 그룹 행동을 할 때 효율적인 연합 행동을 할 수 있도록 인공 포텐셜 함수(Artificial Potential Function, 이하 APF)를 사용하였다. 제안된 연구에서는 균일한 에이전트간의 포메이션 형성, 신속한 목표물 이동, 그리고 에이전트간의 충돌 회피를 만족시키는 동적 연합(Dynamic Association, 이하 DA)알고리즘을 소개 한다. 동적 연합을 바탕으로 조직화된 단일 연합법(Systematic Singular Association, 이하 SSA)을 제안하였다. 제안된 계획에서는 장애물과 목표물 사이에도 직선시야(Line Of Signt, 이하 LOS)를 고려했다. 제안된 SSA 규칙과의 비교를 위해, 에이전트 간의 LOS만 고려하는 근거리 에이전트 선택 단일연합(Singular Association, 이하 SA)과 다(多) 연결 에이전트 선택 SA 알고리즘을 사용하였다. 비교의 결과로 제안된 방법에서 두개의 중요한 장점을 확인했다. 첫째, SSA규칙은 동료 에이전트를 잃을 가능성이 상당히 낮고 빠른 에이전트들의 빠른 이동을 만족시킨다. 둘째, 장애물과 목표물 사이의 LOS고려로 인해서 SSA규칙의 간소화는 특히 그룹 이동시 유리하다. 제안된 알고리즘의 효율성을 자세히 보여주기 위하여 다른 알고리즘과의 비교 시뮬레이션을 제공한다.

• 한국항공우주학회지
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• 제42권9호
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• pp.752-761
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• 2014

뛰어난 기동성으로 인한 최근 쿼드로터에 대한 관심의 증대는 다양한 형태의 멀티로터 비행체의 개발 및 상용화를 이끌었으며, 레져용으로 사용자들이 쉽게 사용할 수 있는 다양한 쿼드로터 제품들이 등장하고 있다. 이 중에서 최근 Parrot사에서 제작한 AR.Drone은 사용자를 위한 내부 안정화 루프를 탑재하고 있기 때문에 초보자들도 쉽게 제어할 수 있는 장점이 있다. 이러한 AR.Drone의 시스템을 이용하면 다양한 형태의 다수 무인기 시스템을 쉽게 구축할 수 있다. 이러한 특성을 바탕으로 한국항공우주연구원에서는 다수의 AR.Drone을 이용한 실내 군집비행 연구를 진행 중에 있다. 이러한 다수의 무인비행체를 이용한 실내 군집비행을 위해서는 개개의 비행체에 대한 위치제어 및 서로간의 충돌을 방지하기 위한 알고리즘이 필요하다. 이를 위해서 본 논문에서는 다수의 실내 무인기의 원활한 기동을 위해 개발한 충돌회피 기동 제어기를 소개하고자 한다.

• The Plant Pathology Journal
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• 제35권5호
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• pp.445-458
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• 2019

The lipopolysaccharide (LPS) composed of lipid A, core, and O-antigen is the fundamental constituent of the outer membrane in gram-negative bacteria. This study was conducted to investigate the roles of LPS in Burkholderia glumae, the phytopathogen causing bacterial panicle blight and seedling rot in rice. To study the roles of the core oligosaccharide (OS) and the O-antigen region, mutant strains targeting the waaC and the wbiFGHI genes were generated. The LPS profile was greatly affected by disruption of the waaC gene and slight reductions were observed in the O-antigen region following wbiFGHI deletions. The results indicated that disruption in the core OS biosynthesis-related gene, waaC, was associated with increased sensitivity to environmental stress conditions including acidic, osmotic, saline, and detergent stress, and to polymyxin B. Moreover, significant impairment in the swimming and swarming motility and attenuation of bacterial virulence to rice were also observed in the waaC-defective mutant. The motility and virulence of O-antigen mutants defective in any gene of the wbiFGHI operon, were not significantly different from the wild-type except in slight decrease in swimming and swarming motility with wbiH deletion. Altogether, the results of present study indicated that the LPS, particularly the core OS region, is required for tolerance to environmental stress and full virulence in B. glumae. To our knowledge, this is the first functional study of LPS in a plant pathogenic Burkholderia sp. and presents a step forward toward full understanding of B. glumae pathogenesis.

• 한국어병학회지
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• 제34권1호
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• pp.105-110
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• 2021

We experienced mortality of dark-banded rockfish (Sebastes inermis) maintained in the rearing facility located in Gangneung, after transportation. Moribund fish showed various symptoms such as exophthalmia, skin ulcers, tail rots, gill rots, discoloration of liver with petechiae, yellowish fluid in intestines and ascites. Two different colonies were dominantly appeared after spreading the lesions on the agar plates and incubation. One isolate (SI_1) showed swarming movement on TSA, and formed yellow colonies on TCBS agar. The other (SI_2) showed no swarming motility and green colonies on TCBS agar. Both of them were Gram-negative. All of these results are similar with those of Genus Vibrio. They were identified as V. harveyi and V. gigantis by PCR with subsequent sequencing of 3 different genes (16 rDNA, recA, rpoA). V. harveyi is well-known as a serious pathogen of marine fish and invertebrates, while V. gigantis is known to be often isolated from marine invertebrates, but the pathogenicity is still unknown. We suspect V. harveyi as the cause of the mortality of dark-banded rockfish, but challenge experiments with these 2 Vibrio species are thought to be necessary to make a clear conclusion.

• The Plant Pathology Journal
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• 제39권1호
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• pp.123-135
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• 2023

Previously, Pseudomonas plecoglossicida YJR13 and Pseudomonas putida YJR92 from a sequential screening procedure were proven to effectively control Phytophthora blight caused by Phytophthora capsici. In this study, we further investigated the anti-oomycete activities of these strains against mycelial growth, zoospore germination, and germ tube elongation of P. capsici. We also investigated root colonization ability of the bacterial strains in square dishes, including cell motility (swimming and swarming motilities) and biofilm formation. Both strains significantly inhibited mycelial growth in liquid and solid V8 juice media and M9 minimal media, zoospore germination, and germ tube elongation compared with Bacillus vallismortis EXTN-1 (positive biocontrol strain), Sphingomonas aquatilis KU408 (negative biocontrol strain), and MgSO₄ solution (untreated control). In diluted (nutrient-deficient) V₈ juice broth, the tested strain populations were maintained at >10⁸ cells/ml, simultaneously providing mycelial inhibitory activity. Additionally, these strains colonized pepper roots at a 10⁶ cells/ml concentration for 7 days. The root colonization of the strains was supported by strong swimming and swarming activities, biofilm formation, and chemotactic activity towards exudate components (amino acids, organic acids, and sugars) of pepper roots. Collectively, these results suggest that strains YJR13 and YJR92 can effectively suppress Phytophthora blight of pepper through direct anti-oomycete activities against mycelial growth, zoospore germination and germ tube elongation. Bacterial colonization of pepper roots may be mediated by cell motility and biofilm formation together with chemotaxis to root exudates.

• 대한약침학회지
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• 제26권4호
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• pp.307-318
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• 2023

Objectives: Bacterial biofilm is regarded as a significant threat to the production of safe food and the arise of antibiotic-resistant bacteria. The objective of this investigation is to evaluate the quorum sensing inhibitory effect of Nepeta curviflora methanolic extract. Methods: The effectiveness of the leaves at sub-inhibitory concentrations of 2.5, 1.25, and 0.6 mg/mL on the virulence factors and biofilm formation of P. aeruginosa was evaluated. The effect of N. curviflora methanolic extract on the virulence factors of P. aeruginosa, including pyocyanin, rhamnolipid, protease, and chitinase, was evaluated. Other tests including the crystal violet assay, scanning electron microscopy (SEM), swarming motility, aggregation ability, hydrophobicity and exopolysaccharide production were conducted to assess the effect of the extract on the formation of biofilm. Insight into the mode of antiquorum sensing action was evaluated by examining the effect of the extract on the activity of N-Acyl homoserine lactone (AHL) and the expression of pslA and pelA genes. Results: The results showed a significant attenuation in the production of pyocyanin and rhamnolipid and in the activities of protease and chitinase enzymes at 2.5 and 1.25 mg/mL. In addition, N. curviflora methanolic extract significantly inhibited the formation of P. aeruginosa biofilm by decreasing aggregation, hydrophobicity, and swarming motility as well as the production of exopolysaccharide (EPS). A significant reduction in AHL secretion and pslA gene expression was observed, indicating that the extract inhibited quorum sensing by disrupting the quorum-sensing systems. The quorum-sensing inhibitory effect of N. curviflora extract appears to be attributed to the presence of kaempferol, quercetin, salicylic acid, rutin, and rosmarinic acid, as indicated by LCMS analysis. Conclusion: The results of the present study provide insight into the potential of developing anti-quorum sensing agents using the extract and the identified compounds to treat infections resulting from quorum sensing-mediated bacterial pathogenesis.

• 대한조선학회논문집
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• 제61권3호
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• pp.170-184
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• 2024

With the advancement of autonomous navigation technology in maritime domain, there is an active research on swarming Unmanned Surface Vehicles (USVs) that can fulfill missions with low cost and high efficiency. In this study, we propose a formation control algorithm that maintains a certain shape when multiple unmanned surface vehicles operate in a swarm. In the case of swarming, individual USVs need to be able to accurately follow the target state and avoid collisions with obstacles or other vessels in the swarm. In order to generate guidance commands for swarm formation control, the potential field method has been a major focus of swarm control research, but the method using the potential field only uses the position information of obstacles or other ships, so it cannot effectively respond to moving targets and obstacles. In situations such as the formation change of a swarm of ships, the formation control is performed in a dense environment, so the position and velocity information of the target and nearby obstacles must be considered to effectively change the formation. In order to overcome these limitations, this paper applies a method that considers relative velocity to the potential field-based guidance law to improve target following and collision avoidance performance. Considering the relative velocity of the moving target, the potential field for nearby obstacles is newly defined by utilizing the concept of Velocity Obstacle (VO), and the effectiveness and efficiency of the proposed method is verified through swarm control simulation, and swarm control experiments using a small scaled unmanned surface vehicle platform.

• 한국전기전자재료학회논문지
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• 제20권9호
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• pp.780-786
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• 2007

This paper describes the influence on partial discharge characteristics of defects at the model XLPE/EPDM interfaces of power cable joints. The defects of void and copper which could inadvertently be present at the joint interface. We measured ${\Phi}-n,\;{\Phi}-q$ patterns by a computer-aided partial discharge measuring system. Several parameters i, e, maximum discharge $q_{max}$ [pC], average discharge q [pC/cycle], and average angle of discharge ${\Theta}g$ [deg] were found to depend upon the defect type varying applied voltage. As the result of time evaluation, partial discharges are small different at copper defects, but is decreasing obviously about 20 minutes at void defect. It considered that difference of magnitude of total positive discharge of Q+ [pC] and total negative discharge of Q- [pC] is SPMD(swarming pulsive micro discharges).