• Title/Summary/Keyword: Surface-applied

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Development of a Matrix-prespotted Plate for Enhancing the Reproducibility of Serum Glycan Analysis by MALDI-TOF-MS

  • Ha, Mi-Young;In, Young-Ha;Maeng, Hye-Sun;Zee, Ok-Pyo;Lee, Jong-Sik;Kim, Yang-Sun
    • Mass Spectrometry Letters
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    • v.2 no.3
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    • pp.61-64
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    • 2011
  • Matrix Assisted Laser Desorption/Ionization-Time-of-Flight mass spectrometry (MALDI-TOF-MS) is the most widely used MS technique for glycan analysis. However, the poor point-to-point and sample-to-sample reproducibility becomes a limit in glycan biomarker research. A prespotted MALDI plate which overcomes the large crystal formation of 2,5-dihydroxybenzoic acid (DHB) has been developed and applied for glycan analysis. A homogeneous matrix coated surface without a crystal structure was formed on a hydrophilic/ hydrophobic patterned surface using a piezoelectric device. The reproducible MALDI-TOF-MS data have been presented using MALDI imaging of beer glycan as well as serum glycan eluted from 10% and 20% ACN elution fractions. The glycan profile from the serum glycan by MALDI-TOF-MS with a DHB prespotted plate was highly conserved for 10 different spectra and the coefficient of variations of significant ion peaks of MALDI data varies from 3.59 to 19.95.

Free surface effects on 2-D airfoils and 3-D wings moving over water

  • Bal, Sakir
    • Ocean Systems Engineering
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    • v.6 no.3
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    • pp.245-264
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    • 2016
  • The iterative boundary element method (IBEM) developed originally before for cavitating two-dimensional (2-D) and three-dimensional (3-D) hydrofoils moving under free surface is modified and applied to the case of 2-D (two-dimensional) airfoils and 3-D (three-dimensional) wings over water. The calculation of the steady-state flow characteristics of an inviscid, incompressible fluid past 2-D airfoils and 3-D wings above free water surface is of practical importance for air-assisted marine vehicles such as some racing boats including catamarans with hydrofoils and WIG (Wing-In-Ground) effect crafts. In the present paper, the effects of free surface both on 2-D airfoils and 3-D wings moving steadily over free water surface are investigated in detail. The iterative numerical method (IBEM) based on the Green's theorem allows separating the airfoil or wing problems and the free surface problem. Both the 2-D airfoil surface (or 3-D wing surface) and the free surface are modeled with constant strength dipole and constant strength source panels. While the kinematic boundary condition is applied on the airfoil surface or on the wing surface, the linearized kinematic-dynamic combined condition is applied on the free surface. The source strengths on the free surface are expressed in terms of perturbation potential by applying the linearized free surface conditions. No radiation condition is enforced for downstream boundary in 2-D airfoil and 3-D wing cases and transverse boundaries in only 3-D wing case. The method is first applied to 2-D NACA0004 airfoil with angle of attack of four degrees to validate the method. The effects of height of 2-D airfoil from free surface and Froude number on lift and drag coefficients are investigated. The method is also applied to NACA0015 airfoil for another validation with experiments in case of ground effect. The lift coefficient with different clearance values are compared with those of experiments. The numerical method is then applied to NACA0012 airfoil with the angle of attack of five degrees and the effects of Froude number and clearance on the lift and drag coefficients are discussed. The method is lastly applied to a rectangular 3-D wing and the effects of Froude number on wing performance have been investigated. The numerical results for wing moving under free surface have also been compared with those of the same wing moving above free surface. It has been found that the free surface can affect the wing performance significantly.

Relation between applied forces and surface characteristics on surface rolling in cast iron (주철의 표면로울링에서 가압력의 영향)

  • 육굉수;박병성;최재승
    • Transactions of the Korean Society of Mechanical Engineers
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    • v.12 no.4
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    • pp.705-711
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    • 1988
  • Surface rolling is one of the plastic deformation processes, which improves surface roughness and increases hardness. The surface rolling tool used in this experiment was specially designed by author for surface rolling on cast iron. The cutting feeds were 0.10mm/rev.and 0.30mm/rev.The applied forces of rolling were in the range of 10kgf to 30kgf with 5kgf differences. The results obtained are as follows. (1) In general the good surface roughness can be obtained with the applied force 25kgf according to surface roughness of the previous turning. (2) The hardness is mainly increased at the first rolling process and is more increased with the increasing applied forces. (3) The reduction of the diameter heavily depends on the original surface roughness. That is, the larger surface roughness is, the larger is the reduction is the reduction of diameter.

Reinforcement effect of surface stabilizer using surface curtain walls on aging reservoirs

  • Song, Sang-Huwon;Cho, Dae-Sung;Seo, Se-Gwan
    • Geomechanics and Engineering
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    • v.28 no.1
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    • pp.1-10
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    • 2022
  • In Korea, accidents related to the collapse of deteriorated aging reservoirs occur every year. The grouting method is generally applied to reinforce an aging reservoir. However, when using this method, different reinforcing effects appear depending on the ground conditions. Thus, new construction methods and materials capable of providing consistent reinforcing effects are required. In this study, the direct shear test (DST), model test, and simulation analysis were performed to evaluate the impact of surface stabilizers, generally used to reinforce roads, rivers, and slopes of roads, applied using surface curtain walls on aging reservoirs. The DST results indicate that when the surface stabilizer was mixed with in-situ soil, the increase in cohesion was the highest at a mixing ratio of 9%. No changes in the friction angle were evident; therefore, 9% was determined to be the optimal mixing ratio. In addition, the model test and simulation analysis showed that when 9% of the surface stabilizer was mixed and applied to the aging reservoir, the seepage quantity of water and the saturated area were reduced by approximately 42% and 73%, respectively. Moreover, the comprehensive analysis of results showed that the grouting method could be completely replaced by surface stabilizers applied through surface curtain walls because the technique could secure stability by decreasing the seepage in the aging reservoir.

TITLE : THE ROLE OF COLLAGEN FIBER IN DENTIN BONDING (치과용복합레진과의 결합에 있어, 상아질 내 교원섬유의 역할에 관한 연구)

  • Park, Seong-Ho
    • Restorative Dentistry and Endodontics
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    • v.22 no.1
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    • pp.470-478
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    • 1997
  • The purpose of this study was to investigate the effects of moistening mechods of dentin on the morphologic states of hybrid layers and on the interfacial bond strength between dentin and composite. Specimens were divided into 6 groups based on the surface moistening methods and materials used. After the dentin surface was conditioned with 10 % phoporic acid and irrigated: 1. The dentin surface was dried. Then bonding agent and composite were applied. 2. The dentin surface was blot-dryed. Then primer, bonding atent and composite were applied. 3. The dentin surface was dryed first. Within 20 seconds, the surface was rewetted, then primer, bonding agent and composite were applied. 4. The dentin surface was dryed. Then primer, bonding agnent and composite were applied. 5. The dentin surface was dryed first. Atter 24hrs, the surface was rewetted, and then primer, bonding agent and composite were applied. 6. The surface was conditioned with NaOCl for 5min. Then primer, bonding agent and composite were applied. To reveal the hybrid layer, scanning electron microscopy was used after the samples were ion beam etched. The shear bond strength of each group was tom pared by ANOVA. In groups 2, 3 and 4, the hybrid layer was clearly visible, but the width was more limited in group 4. In group 1 and 5, the hybrid layer was not found, and a gap was formed between dentin and composite. In group 6, the hybrid layer was not found, but the interface between the dentin and composite was intimate. The shear bond strength of each group was as follows: Group 1 : 4MPa, Group 2 : 14MPa, Group 3 : 12MPa, Group 4 : 14MPa, Group 5 : 5MPa, Group 6 : 9MPa.

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Surface hardness measurement of NiP-plated AA7050

  • Moon, Sungmo;Kim, Juseok
    • Journal of Surface Science and Engineering
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    • v.54 no.4
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    • pp.171-177
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    • 2021
  • This paper is concerned with the surface hardness measurement of NiP-coated AA7050 using different loads from 10 to 100 g. The surface hardness was observed to increase from 180 to 600 Hv with increasing NiP layer thickness, depending on the load applied for indentation. When NiP coating thickness is thinner than 2 ㎛, the surface hardness of NiP-coated AA7050 was mainly determined by AA7050 substrate, while it was significantly increased by NiP coating layer when NiP coating thickness is thicker than 2 ㎛. Hardness of AA7050 substrate itself was not dependent on the applied load but the hardness of NiP-coated AA7050 was largely influenced by the load applied for indentation. The largest difference of hardness between 10 g and 100g of applied loads, was obtained at the NiP thickness of about 8 ㎛ above which the measured hardness at 10 g reached a maximum value of about 600 Hv. It was also observed that indentation-induced plastic deformation next to the indented zone occurs when NiP layer is 5.64 times thicker than the depth of impression formed by indentation.

EXPERIMENTAL STUDIES ON THE SURFACE ROUGHNESS OF GLASS IONOMER CEMENT RESTORATIONS (Glass Ionomer Cement 수복물(修復物)의 표면거칠기에 관한 실험적 연구)

  • Kim, Kwang-Soon;Lee, Seung-Jong;Lee, Chung-Suck
    • Restorative Dentistry and Endodontics
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    • v.17 no.1
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    • pp.166-180
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    • 1992
  • One disadvantage of Glass Ionomer Cement Restoration is the difficulty in polishing. To find the appropriate polishing method, we polished the surface of Glass Ionomer Cement Restorations by 11 combination methods serially using disks shared with large-small particles and evaluated the polishing process in terms of surface roughness, surface roughness curve, and SEM findings. In addition, a visible light curing type bonding material was applied to evaluate the possible improvement in surface properties. The following results were obtained. 1. The disk surface of Glass Ionomer Cement was polished serially by disks with superfine particles, but it didn't become smooth. 2. The surface of Microfilled Composite resin became smoother as using a disk with finer particles. 3. When a visible light curing type bonding material was applied in finishing process, the surface of Glass Ionomer Cement became smooth as much as the applied matrix.

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PROPAGATION OF SURFACE WAVES ON IRREGULAR BED TOPOGRAPHY

  • WARKE A. S.;DAS S. K.;DEBNATH L.
    • Journal of applied mathematics & informatics
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    • v.20 no.1_2
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    • pp.197-208
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    • 2006
  • This paper deals with the exact solution of surface gravity waves in an ocean with irregular bed topography. In order to obtain water surface elevation and run-up of infra-gravity waves when the bed is either wavy or exponential, closed form solutions are obtained. Numerical computations indicate that when solitary wave or sinusoidal wave conditions are applied at the boundary, water surface elevation attains near Gaussian profile.