• Title/Summary/Keyword: Surface migration

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Occurrence, Seasonal Changes and Vertical Distribution of Silica-scaled Chrysophytes in a Small Fish-free Pond in Japan

  • Kim, Han-Soon;Noriko Takamura
    • Animal cells and systems
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    • v.5 no.2
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    • pp.117-126
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    • 2001
  • Occurence, seasonal changes and vertical distribution of the silica-scaled chrysophytes in a small fish-free pond were studied using electron microscopy (EM) and light microscopy (LM) from October 1998 to July 1999. The phytoplankton community was characterized by Chrysophyta and Cryptophyta. Ten species of the silica-scaled chrysophytes of genera Mallomonas, Synura, Chrysosphaerella and Spiniferomonas were identified by EM, and the most abundant species were Mallomonas akrokomos and M. portae-ferreae. The maximum population density of M. akrokomos was observed in December and several peaks appeared periodically at about one to two month intervals, whereas M. portae-ferreae developed the maximum density in March. The diurnal vertical distribution of M. akrokomos exhibited clear downward migration at night and slightly upward migration in the morning. A complex interaction among physico-chemical and biological factors seemed to affect the vertical distribution of M. akrokomos. However, the seasonal changes of M. akrokomos did not show significant corre1ations with the physico-chemical and some biologica1 factors. Although M. akrokomos was evenly distributed throughout the water column during some experimental periods, the diurnal patterns found in the two diurnal cycles showed consistency in that it clearly avoided the surface water during the day. This suggested that M. akrokomos may be a shade plankton with maximum densities below surface layer.

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Bacitracin Inhibits the Migration of U87-MG Glioma Cells via Interferences of the Integrin Outside-in Signaling Pathway

  • Li, Songyuan;Li, Chunhao;Ryu, Hyang-Hwa;Lim, Sa-Hoe;Jang, Woo-Youl;Jung, Shin
    • Journal of Korean Neurosurgical Society
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    • v.59 no.2
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    • pp.106-116
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    • 2016
  • Objective : Protein disulfide isomerase (PDI) acts as a chaperone on the cell surface, and it has been reported that PDI is associated with the tumor cell migration and invasion. The aims of this study are to investigate the anti-migration effect of bacitracin, which is an inhibitor of PDI, and the associated factor in this process. Methods : U87-MG glioma cells were treated with bacitracin in 1.25, 2.5, 3.75, and 5.0 mM concentrations. Western blot with caspase-3 was applied to evaluate the cytotoxicity of bacitracin. Adhesion, morphology, migration assays, and organotypic brain-slice culture were performed to evaluate the effect of bacitracin to the tumor cell. Western blot, PCR, and gelatin zymography were performed to investigate the associated factors. Thirty glioma tissues were collected following immunohistochemistry and Western blot. Results : Bacitracin showed a cytotoxicity in 3rd (p<0.05) and 4th (p<0.001) days, in 5.0 Mm concentration. The cell adhesion significantly decreased and the cells became a round shape after treated with bacitracin. The migration ability, the expression of phosphorylated focal adhesion kinase (p-FAK) and matrix metalloproteinase-2 (MMP-2) decreased in a bacitracin dose- and time-dependent manner. The U87-MG cells exhibited low-invasiveness in the 2.5 mM, compared with the untreated in organotypic brain-slice culture. PDI was expressed in the tumor margin, and significantly increased with histological glioma grades (p<0.001). Conclusion : Bacitracin, as a functional inhibitor of PDI, decreased the phosphorylated FAK and the secreted MMP-2, which are the downstream of integrin and play a major role in cell migration and invasion, might become one of the feasible therapeutic strategies for glioblastoma.

Dominant Migration Element in Electrochemical Migration of Eutectic SnPb Solder Alloy in D. I. Water and NaCl Solutions (증류수 및 NaCl 용액내 SnPb 솔더 합금의 Electrochemical Migration 우세 확산원소 분석)

  • Jung, Ja-Young;Lee, Shin-Bok;Yoo, Young-Ran;Kim, Young-Sik;Joo, Young-Chang;Park, Young-Bae
    • Journal of the Microelectronics and Packaging Society
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    • v.13 no.3 s.40
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    • pp.1-8
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    • 2006
  • Higher density integration and adoption of new materials in advanced electronic package systems result in severe electrochemical reliability issues in microelectronic packaging due to higher electric field under high temperature and humidity conditions. Under these harsh conditions, metal interconnects respond to applied voltages by electrochemical ionization and conductive filament formation, which leads to short-circuit failure of the electronic package. In this work, in-situ water drop test and evaluation of corrosion characteristics for SnPb solder alloys in D.I. water and NaCl solutions were carried out to understand the fundamental electrochemical migration characteristics and to correlate each other. It was revealed that electrochemical migration behavior of SnPb solder alloys was closely related to the corrosion characteristics, and Pb was primarily ionized in both D.I. water and $Cl^{-}$ solutions. The quality of passive film formed at film surface seems to be critical not only for corrosion resistance but also for ECM resistance of solder alloys.

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Migration Patterns of Brachymystax lenok tsinlingensis Using Radio Tags in the Upper Part of the Nakdong River (Radio tag을 이용한 낙동강 상류에 서식하는 열목어의 이동양상)

  • Yoon, Ju-Duk;Jang, Min-Ho
    • Korean Journal of Ecology and Environment
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    • v.42 no.1
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    • pp.58-66
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    • 2009
  • The telemetry of eight adult manchurian trouts (Brachymystax lenok tsinlingensis) in the upper part of the Nakdong River, which is the southern limit of distribution of manchurian trout on the Korean peninsula, was used to examine migration patterns and evaluate characteristics of over-wintering and the spawning season between December, 2007 and May, 2008. Based on the tracking data, the tagged fish showed a limited migration between adjacent pools, moving only up to $8.6m\;day^{-1}$ during the winter season (December to February). Hydraulic conditions of over-wintering pool areas were, ca. 1m depth, slow moving surface water with areas of sand and gravel. The migration of tagged individuals was successful, moving up to $96.2m\;day^{-1}$ during the spawning season. Two tagged individuals (BL4, BL6) exhibited upstream migration, whereas others showed downstream movements. The timing of upstream migration of the two individuals was consistent with an increasing phases of water level and discharge. The fishes migrating toward the down stream moved to the wide pool areas downstream, where they spent the summer season for the growth.

Application of TREECS Program to Predict the Fate of TNT and RDX from Firing Range (TREECS 프로그램을 이용한 화약류 오염 군 사격장 토양의 TNT와 RDX 유출 특성 연구)

  • Yu, Gihyeon;Jung, Jae-Woong;Nam, Kyoungphile
    • Journal of Soil and Groundwater Environment
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    • v.20 no.6
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    • pp.133-139
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    • 2015
  • Attention to munitions constituents such as 2,4,6-trinitrotoluene (TNT) and hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) in the firing ranges is increasing due to their toxicity and high mobility to the environment. It is helpful to use a systemic model to predict the amount of contaminants for the establishment of environmental management of firing ranges. This study employed Training Range Environmental Evaluation and Characterization System (TREECS) program to estimate the mobility characteristics of TNT and RDX via groundwater leaching, soil erosion and surface water runoff. The prediction results of the TNT and RDX migration with TREECS showed that 68% of initial TNT and 21% of initial RDX were discharged through the soil erosion and the 20% of initial TNT and 54% of initial RDX ran out the firing range via the groundwater leaching. The rest of the initial TNT and RDX moved to adjacent surface water via surface runoff. The data suggest that soil erosion and surface runoff occupying 80% of TNT to the total amount are important migration pathways. On the other hand, groundwater leachning occupying 54% to the total amount was also important pathway for RDX.

Enzymatic reaction model for the production of symmetrical lipid molecules using the response surface methodology

  • Hong, Joon-Sun;Shin, Jung-Ah;Lee, Ki-Teak
    • Korean Journal of Agricultural Science
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    • v.45 no.2
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    • pp.265-282
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    • 2018
  • The purpose of this research was to produce symmetric (Saturated-Unsaturated-Saturated, SUS) triacylglycerol (TAG) using palm stearin fraction oil (PSFO) and high oleic sunflower oil (HOSO) as substrates to replace cocoa butter. PSFO was blended with HOSO (1 : 2 weight ratio), and $lipozyme^{(R)}$ TLIM (20 weight % of the substrate) was added. Interesterification was carried out in a shaking water bath at $55^{\circ}C$ at 220 rpm for 6 hours. The response surface methodology (RSM) through the central composite face design was employed to observe the optimized SUS-TAG. The independent factors were the reaction temperature ($X_1$: 65, 75 and $85^{\circ}C$), reaction time ($X_2$: 1, 3 and 5 hours) and ratio of TLIM ($X_3$: 10, 15 and 20 weight %). The dependent variables were $Y_1$ = Saturated-Unsaturated-Unsaturated (SUU, area %), $Y_2=SUS$ (area %), $Y_3$ = Saturated-Saturated-Unsaturated (SSU, area %), $Y_4$ = Unsaturated-Unsaturated-Unsaturated (UUU, area %), and $Y_5=sn-2$ unsaturated fatty acid (area %). The optimal conditions from the central composite face design minimized acyl migration while maximizing the presence of unsaturated fatty acid at the sn-2 position (73.43 area %). The optimal conditions were $X_1=65^{\circ}C$, $X_2=1hour$, and $X_3=20weight%$. As a result of the response surface analysis, the lack of fits was found as $Y_1=0.622$, $Y_2=0.438$, $Y_3=0.264$, $Y_4=0.526$, and $Y_5=0.215$, and their $R^2$ were 0.897, 0.944, 0.826, 0.857, and 0.867, respectively.

Patterns of Vertical Distribution and Diel Vertical Migration of Zooplankton in the East Sea of Korea (Sea of Japan)

  • Park, Chul;Lee, Chang-Rae;Hong, Sung-Yun
    • Journal of the korean society of oceanography
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    • v.32 no.1
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    • pp.38-45
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    • 1997
  • To find out the changes in vertical distribution patterns over the 24-h period, a key and the first step to tackle the problem of adaptive significance of diel vertical migration (DVM), vertically stratified time series samplings with multiple opening/closing plankton samplers were done in the East Sea of Korea (Sea of Japan). Sampling was done almost every 4 h for one day period following the same water parcel in Nov. 1995 and May 1996, respectively. Resultant patterns of vertical distribution showed that some species such as most abundant taxa Metridia pacifica and Scolecithyicella minor, both Copepoda, performed DVM even in the study area of strong thermal stratification. Their patterns of DVM such as distance scales and timing of movements were not the same each other, and they were separated from other taxa in the dendrogram obtained by the cluster analyses, Most minor taxa grouped in one, however, seemed not to do DVM in the study area of strong thermal stratification. They usually preferred the warmer surface layer where the foods were probably more abundant.

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Sintering Behaviors of ITO Ceramics with Additions of TiO$_2$ (TiO$_2$첨가에 따른 ITO 세라믹스의 소결 거동)

  • 정성경;김봉철;장세홍;김정주
    • Journal of the Korean Ceramic Society
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    • v.35 no.4
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    • pp.347-354
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    • 1998
  • Densification and grain growth behaviors of ITO ceramics were investigated as a function of TiO2 ad-ditions. TiO2 addition led to inhibition of the grain growth and promotion of the densification of ITO ceram-ics. From the microstructure observation it was found that the crack-like voids which were formed in pure ITO specimens decreased with increase of TiO2 additon. The grain growth exponent(n) was measur-ed to be 4 for pure ITO 3 for TiO2-doped ITO specimens respectively. It was supposed that the grain boun-dary migration of pure ITO ceramics was controlled by the pores which were moved by surface diffusion. On the contrary the grain boundary migration of TiO2-doped ITO specimens was depressed by solute drag effect. The activation energies for grain growth were measured to be 1013 kJ/mol for pure ITO ceramics and 460kJ/mol for TiO2-doped ITO specimens respectively.

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The Vertical Distribution and Diurnal Migration of Cladocera, Evadne Nordmanii Loven at Different Stations in the Irish Sea.

  • Lee, Jong Wha
    • 한국해양학회지
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    • v.9 no.2
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    • pp.1-9
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    • 1974
  • The vertical distribution and diurnal migration of Evadne nordmanii has been studied at different stations in the Irish Sea. There are many reports that the migrations of planktonic animals tend to be hampered by thermoclines and haloclines. Physical and chemical factors were compared with vertical distribution and diurnal migrations of the animal. Evadne must be essentially an epiplanktonic form with the ability to endure strong light intensity. The animals generally migrated to the very surface layer from their shallow day strata with the decreased of light intensity. During the night their distribution seems rather random or even throughout all the layers, with a tendency to concentrate at the bottom layers, when the water was homogeneous physically and chemically. E. nordmanii may penetrate minor thermoclines and haloclines, but they may avoid chemically distinct waters or possibly currents.

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Study on Diffusion Bonding of Stainless Steel to Mild Steel (연강-스테인리스강의 확산접합에 관한 연구)

  • Kim, S.T.
    • Journal of the Korean Society for Heat Treatment
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    • v.11 no.1
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    • pp.17-26
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    • 1998
  • Cladding of stainless steel on mild steel was prepared by diffusion bonding process. The bond strength increased with an increase of bonding temperature and time. It was also found that the bond strength increased as the surface roughness decreased. After the diffusion bonding of stainless steel-mild steel, the mild steel part near the bonded interface showed higher strength than the base steel due to the migration of chromium and nickel from stainless steel to mild steel. Carbon migration from mild steel gave effect on the formation of chromium carbides at grain boundaries of stainless steel, the fractograpohic features of the imperfectly bonded interface showed rather coarse dimples in the mild steel part and very fine dimples in the stainless steel part.

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