• Asian Pacific Journal of Cancer Prevention
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• 제16권7호
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• pp.2793-2800
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• 2015

In molecular-targeted cancer therapy, acquired resistance to gemcitabine is a major clinical problem that reduces its effectiveness, resulting in recurrence and metastasis of cancers. In spite of great efforts to reveal the overall mechanism of acquired gemcitabine resistance, no definitive genetic factors have been identified that are absolutely responsible for the resistance process. Therefore, we performed a cross-platform meta-analysis of three publically available microarray datasets for cancer cell lines with acquired gemcitabine resistance, using the R-based RankProd algorithm, and were able to identify a total of 158 differentially expressed genes (DEGs; 76 up- and 82 down-regulated) that are potentially involved in acquired resistance to gemcitabine. Indeed, the top 20 up- and down-regulated DEGs are largely associated with a common process of carcinogenesis in many cells. For the top 50 up- and down-regulated DEGs, we conducted integrated analyses of a gene regulatory network, a gene co-expression network, and a protein-protein interaction network. The identified DEGs were functionally enriched via Gene Ontology hierarchy and Kyoto Encyclopedia of Genes and Genomes pathway analyses. By systemic combinational analysis of the three molecular networks, we could condense the total number of DEGs to final seven genes. Notably, GJA1, LEF1, and CCND2 were contained within the lists of the top 20 up- or down-regulated DEGs. Our study represents a comprehensive overview of the gene expression patterns associated with acquired gemcitabine resistance and theoretical support for further clinical therapeutic studies.

• Physical Therapy Rehabilitation Science
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• 제5권2호
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• pp.106-112
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• 2016

Objective: This study was conducted to investigate the effects of resistant exercise on the gait performance of a patient with systemic lupus erythematosus (SLE) patient. Design: A case study. Methods: A 30-year-old male adult who had been diagnosed with systemic lupus erythmatosus (SLE) in April 2013, right middle cerebral artery infarction, and with left hemiplegia agreed to participate in this case study. Patient was unable to walk due to being affected with adynamia. Due to developing necrotizing vasculitis on the left lower extremity, patient underwent a myotomy on the left thigh. The patient was trained with a progressive resistant exercise program for 8 weeks. An intensity of 15 RM was used for the resistant exercises and the resistance level was increased progressively in order to improve the muscle power of the patient. Methods used to increase resistance included changing positions, providing mechanical resistance instead of manual resistance, transitioning from open kinetic chain to closed kinetic chain exercises, and changing the colors of the theraband to those with increase level of resistance. Outcome measures included the 5-repetition sit-to- stand test (5RSST), Timed Up & Go (TUG), and 10-meter walk test (10MWT). In addition, the GAITRite was used to assess the spatio-temporal gait variables, including gait speed, cadence, stride length of the left side, and double limb support pre and post-intervention. Results: The patient was able to perform sit-to- stand after two weeks of performing the resistant exercises. The patient was able to walk after 4 weeks, and the patient's overall gait performance had improved after 8 weeks. All of the variables had improved after each week. Conclusions: The results of this case study may be used to enhance future efforts to objectively evaluate resistant exercises during gait performance in persons affected by SLE.

• 한국자동차공학회논문집
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• 제22권4호
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• pp.30-35
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• 2014

According to weight lightning trend of automobile body, necessity of resistance spot welding (RSW) of Mg alloy on automobile industry is increasing rapidly because of the highest specific strength among commercial metals. Mg alloy has low electric resistance and high thermal conductivity like as Al alloy, so that needs applying high current in short time when welding. Thick oxide film of Mg alloy pollutes the electrodes and makes partial current carrying paths when on welding. Partial current carrying paths signify excessive concentration of current. There can initiate expulsion easily and reduces electrode life rapidly. Generating partial current carrying paths and expulsions are influenced by shapes of electrode. Therefore, electrode life also influenced by shape. In this study, we perform life test of RSW electrode of radius type. Measure tensile shear load and nugget size every spot alternately. As a result, radius type electrode can extend life over twice as dome type electrode.

• 한국산학기술학회논문지
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• 제18권7호
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• pp.483-489
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• 2017

본 연구의 목적은 직물의 발수도 판정 (KS K 0590) 시 이미징 기반의 판별을 통해서 측정 신뢰도를 향상하는 것으로 한다. 이를 수행하기 위하여 가장 먼저 우리는 Java 기반의 이미지 처리 프로그램을 통해서 발수도 판정 표준표를 정량화하였다. 이때 모든 이미지의 처리과정은 미 국립보건원(NIH)에서 개발한 오픈 소프트웨어인 Image J를 사용하였다. 발수도 판정 표준표(standard spray test rating)에 대한 이미지 처리과정은 면적 측정 기법을 통해서 수행하였으며, 이를 통해서 정량화한 결과, 습윤 면적비 수치를 기반으로 하는 손쉬운 판정기준을 확보 할 수 있었다. 또한 실제 직물에 있어서 이미징 기반의 발수도 판정을 도입하기 위한 최적화 처리기법을 도출하기 위하여 형광물질을 사용하였다. 형광 이미지를 도입하여 문턱값 (Threshold) 조절과 2치이미지 (Binary Image) 변환 등의 과정을 통해서 발수도 판정을 위한 데이터 처리과정을 진행하였다. 본 연구의 결과물은 향후 직물의 발수도 판정 (KS K 0590)에 있어서 기존의 정성적인 분석법에서 탈피하여 정량적인 측정 신뢰도 향상을 위한 방안으로 적용할 수 있으리라 판단된다.

• 대한토목학회논문집
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• 제34권3호
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• pp.919-930
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• 2014

본 연구에서는 화이버 단면 요소를 이용하여 강재 보강된 숏크리트 합성부재의 하중지지력과 거동을 수치해석적으로 평가하였다. 강재 보강된 숏크리트 합성단면은 여러 개의 화이버로 분할되고, 각 화이버에 정의된 비선형 응력-변형률 관계에 의해 내력을 결정하게 된다. 사용된 유한요소모델의 검증을 위해 수치해석에 의한 숏크리트 라이닝의 하중-변위 변화를 기존 실험연구결과와 비교하였고, 이를 수치해석에 의한 강재와 숏크리트의 응력분포를 이용하여 함께 분석하였다. 그 결과 제안된 해석방법이 강지보와 숏크리트의 재료 비선형성을 고려하여 전체 거동과 강재 및 숏크리트 각각의 하중 저항력을 실질적으로 평가할 수 있음을 보였다. 또한, 단면 내 응력분포로부터 중립축 변화와 강재 및 숏크리트 각각의 휨 하중 분담률을 도출하였다. 하중 변화에 따른 강재의 휨 하중 분담률 변화를 확인하였고, 이를 통해 숏크리트 라이닝 설계에 강재의 휨 저항성능을 고려하는 것이 필요하다고 판단하였다.

• Korean Chemical Engineering Research
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• 제58권4호
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• pp.524-529
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• 2020

고분자전해질 연료전지의 기계적 내구성을 높이기 위해 고분자막에 지지체를 넣은 강화막이 사용되고 있다. 지지체는 주로 e-PTFE를 사용하는데 소수성이고 이온전달이 안되므로 성능저하의 원인이 될 수 있다. 그래서 본 연구에서는 e-PTFE 지지체가 PEMFC 성능과 전기화학적 내구성 미치는 영향에 대해 연구하였다. 본연구에서는 지지체가 들어간 강화막과 들어가지 않은 단일막(비강화막)을 비교하였는데, 지지체의 소수성 때문에 강화막의 물 확산계수가 단일막보다 낮았다. 강화막은 물확산 계수가 낮아 이온의 막 이동 저항이 단일막보다 높았다. 지지체의 낮은 수소투과도 때문에 강화막의 OCV가 단일막보다 높았다. 지지체가 수소투과도를 감소시킴으로서 라디칼 발생속도를 감소시켜 강화막의 전기화학적 내구성도 향상시킴을 보였다.

• 대한토목학회논문집
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• 제32권2B호
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• pp.149-152
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• 2012

본 연구는 해상풍력발전기 기초 설계에 사용되는 IEC 61400-3, DNV-OS-J101, GL Wind, EUROCODE, AASHTO 및 국내 설계기준의 설계방법 및 안전율의 정도를 비교, 분석함으로써, 국내에서 해상풍력발전기 기초 설계시 필요한 제반사항을 제공하고자 한다. 해상풍력발전기 기초 설계에 관한 국내외 설계기준을 분석한 결과 설계법은 크게 설계접근법, 하중저항 설계법, 허용응력설계법을 적용하고 있으며, 각 설계법에 따른 안전율 정도를 분석한 결과 하중저항계수 설계법과 설계접근법은 거의 유사한 수준의 안전율을 확보하고 있는 반면, 허용응력설계법에서는 다소 보수적인 안전율을 적용하고 있어 해상풍력발전기 기초의 경제적 설계를 위한 국내 설계기준 개발이 필요할 것으로 판단된다.

• 한국도로학회논문집
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• 제19권4호
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• pp.27-36
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• 2017

PURPOSES : The purpose of this study is to perform a reliability analysis of the proposed wind load combination which governs the design of support structures of subsidiary road facilities, and to evaluate whether the target reliability of the design is satisfied. METHODS : The statistical estimation method is applied and the design period of the support structure is used to obtain the statistical property of the wind load. In addition, the statistical properties of the strength of support structures are obtained from a literature review and simulation study. Actual support structures are designed by the proposed load combination and are used as the examples to examine if the target reliability is obtained. RESULTS : The result of the reliability analysis performed by using the statistical properties of load and resistance for the support structure in this study indicates that the proposed wind load combination satisfied the target reliability index of the design. Also, the convenience of the design is achieved by adopting the same design wind velocity given in the bridge design code by applying the wind velocity ratio defined for the design period of the support structure. CONCLUSIONS : It is presented that the design using the wind load combination proposed in this study achieved the target reliability index and the design wind load for different design periods can be conveniently defined by applying the velocity ratio proposed in this study.

• The Plant Pathology Journal
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• 제30권2호
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• pp.220-227
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• 2014

The GacS/GacA system in the root colonizer Pseudomonas chlororaphis O6 is a key regulator of many traits relevant to the biocontrol function of this bacterium. Proteomic analysis revealed 12 proteins were down-regulated in a gacS mutant of P. chlororaphis O6. These GacS-regulated proteins functioned in combating oxidative stress, cell signaling, biosynthesis of secondary metabolism, and secretion. The extent of regulation was shown by real-time RT-PCR to vary between the genes. Mutants of P. chlororaphis O6 were generated in two GacS-regulated genes, trpE, encoding a protein involved in tryptophan synthesis, and prnA, required for conversion of tryptophan to the antimicrobial compound, pyrrolitrin. Failure of the trpE mutant to induce systemic resistance in tobacco against a foliar pathogen causing soft rot, Pectobacterium carotovorum SCCI, correlated with reduced colonization of root surfaces implying an inadequate supply of tryptophan to support growth. Although colonization was not affected by mutation in the prnA gene, induction of systemic resistance was reduced, suggesting that pyrrolnitrin was an activator of plant resistance as well as an antifungal agent. Study of mutants in the other GacS-regulated proteins will indicate further the features required for biocontrol-activity in this rhizobacterium.

• PNF and Movement
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• 제14권1호
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• pp.49-52
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• 2016

Purpose: The purpose of the present study was to examine the effects of the area of the base of support formed by the human body on the maximal voluntary isometric contraction of upper limb muscles. Methods: The study was conducted with 20 normal adults. To identify changes in the base of support, the maximal voluntary isometric contraction of the biceps muscle was measured in a standing position, a sitting position, and a lying position for each subject. The sizes of the base of support formed in the standing, sitting, and lying positions were set to 1, 2, and 3 respectively, based on the sizes, to analyze the correlations. The maximal voluntary isometric contraction of the biceps muscle was measured using surface electromyograms (EMGs) (Noraxon DTS, Germany). Results: The results showed negative correlations in which, as the size of the base of support increased, the maximal voluntary isometric contraction of the biceps muscle decreased. Conclusion: Changes in the base of support of the body affect the maximal voluntary isometric contraction of the upper limbs. Therefore, when resistance exercises are applied for muscle strengthening, the positions should be changed considering the changes in muscle activity according to those positions. In addition, when EMGs are used to measure the maximal voluntary isometric contraction, the measurements should be conducted in the same positions, considering muscle activity that changes according to the base of support and positions, for data quantification.