• Title/Summary/Keyword: Supercritical extract

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Increase of Epigallocatechin in Green Tea Extract by Lactic Acid Bacteria Fermentation (젖산균 발효를 통한 녹차 추출물의 Epigallocatechin 함량의 증대)

  • Choi, Chan-Yeong;Park, Eun-Hee;Ju, Yoong-Woon;Kim, Myoung-Dong
    • Microbiology and Biotechnology Letters
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    • v.44 no.1
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    • pp.62-67
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    • 2016
  • Hydrolytic enzyme activities, including those of ${\beta}$-glucosidase, ${\beta}$-glucuronidase, ${\beta}$-xylosidase, ${\beta}$-galactosidase, ${\beta}$-arabinofuranosidase, ${\beta}$-arabinosidase, and ${\beta}$-arabinopyranosidase, which are useful for bioconversion, were explored in lactic acid bacteria isolated from Korean traditional fermented foods. Nine bacterial strains were selected for the fermentation of green tea extract prepared by supercritical fluid extraction. Changes in the concentrations of catechin, epicatechin, epicatechin gallate, epigallocatechin, and epigallocatechin-3-gallate in green tea extract were investigated after fermentation by the selected lactic acid bacteria strains. The strain Leuconostoc mesenteroides MBE1424, which showed the highest ${\beta}$-glucuronidase enzyme activity among the tested bacterial strains, increased the epigallocatechin content of the green tea extract by 60%. In addition, L. mesenteroides MBE1424 was more resistant than the control strain at high temperature and showed a maximum specific growth rate at $40^{\circ}C$. L. mesenteroides MBE1424 was presumed to have an enzyme system containing ${\beta}$-glucuronidase with utility in the bioconversion of green tea extract.

A Study on Whitening and Anti-inflammatory Effects of Eriobotrya Japonica Leaf Extracts with Different Extraction Methods (추출 방법에 따른 비파엽 추출물의 미백 및 항염활성에 관한 연구)

  • Park, Jung Ok;Park, Jin Oh;Joo, Chul Gue
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.41 no.2
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    • pp.151-157
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    • 2015
  • In this study, we prepared Eriobotrya japonica leaf extracts by several extraction processes and then evaluated their biological activities for their potential application as a new raw material of functional cosmetic. Their whitening effects were measured by tyrosinase inhibitory activities, and anti-inflammatory effects were determined by inhibitory activities of nitric oxide (NO) production. Among the several extracts obtained from E. japonica leaf, supercritical fluid extract showed tyrosinase inhibitory activities at the concentration of 10%. Inhibitory activity on NO production effect related to anti-inflammatory efficacy was in the order: supercritical fluid extract > ethanol extract > hot water extract. According to the results of MTT assay, cell cytotoxicity was not observed at all concentrations except for a 5% concentration of the 70% ethanol extract. For whitening effects, 30% ethanol and 70% ethanol extract showed mushroom tyrosinase inhibitory activity at the concentration of 5%. These results indicated that E. japonica leaf extracts could have the functional effects when they are added as ingredients in cosmetics.

Supercritical $CO_2$ Extraction of Camptothecin and 10-hydroxycamptothecin from Camptotheca acuminata (초임계 $CO_2$를 이용한 Camptotheca acuminata에서 Camptothecin 및 10-hydroxycamptothecin 추출)

  • 부성준;변상요
    • KSBB Journal
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    • v.16 no.1
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    • pp.66-70
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    • 2001
  • Factors affecting the supercritical carbon dioxide extraction of camptothecin(CPT) and 10-hydroxycamptothecin(HCPT) from the dried powder of Cmptotheca acuminata were studied. Only a few amount of CPT and HCPT was extracted with pure supercritical carbon dioxide. Methanol and ethanol were efficient modifiers to extract CPT and HCPT. At $40^{\circ}C$, 250 bar, 1 mL/min flow rate, 41% of CPT and 35% of HCPT were extracted with supercritical carbon dioxide modified with 16% of methanol. The diffusion effect of HCPT on extraction efficiency was studied in this solid-fluid system. Round matrix hot-ball model assumption revealed that the value of D/$r^2$ was 0.0072 $min^{-1}$ which was higher than that of solvent extraction with methanol.

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Extraction Efficiencies of Organochlorine Pesticides Spiked in Fish Tissues by Supercritical Carbon Dioxide (초임계이산화탄소에 의한 어류조직 중 유기염소계 농약의 추출수율)

  • Lim, Sang-Bin;Jwa, Mi-Kyung;Kang, Soon-Bae
    • Korean Journal of Food Science and Technology
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    • v.28 no.3
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    • pp.475-481
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    • 1996
  • A simple, fast, inexpensive method hal been developed to extract organochlorine pesticide spiked in fish tissues by supercritical carbon dioxide. Following static supercritical fluid extraction (SFE), pesticides are collected by rapidly depressurizing the carbon dioxide effluent through a 0.79 mm i.d. metering valve into a capped screw-top tube. Percent recovers of standard organochlorine pesticides mixture (SOPM) increased with the decrease of the extraction temperature at 103 bar. Maximum average percent recovery of SOPM was 98% at $40^{\circ}C/172\;bar$ Average recoveries of SOPM spiked in Pacific cod, flatfish and common squid were 85,74,and 83% respectively at $40^{\circ}C/172\;bar$ Application of supercritical carbon dioxide ectraction offers and attractive alternative to the use of organic solvents for extraction of organochlorine pesticides from fish tissues.

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Supercritical Fluid Extraction of Safflower Yellow Pigments from Carthamus tinctorius L. (초임계 이산화탄소를 이용한 홍화로부터 황색소 추출)

  • Han, Byung-Seok;Kim, Kong-Hwan;Chung, In-Sik
    • Applied Biological Chemistry
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    • v.41 no.5
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    • pp.363-366
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    • 1998
  • Supercritical fluid(SCF) carbon dioxide was used to extract safflower yellow pigments from Carthamus tinctorius L. In this work, supercritical fluid extractions were performed at various conditions; pressure (2000, 3000, 4000, 5000 psig), temperature $(40,\;50,\;60,\;70,\;80^{\circ}C)$ and co-solvent $(0,\;3,\;6,\;10,\;14\;wt%\;H_2O)$. Total concentrations of safflower yellow pigments extracted were determined by spectrophotometric method. A maximum yield of yellow pigments was obtained at 4000psig, $60^{\circ}C$ and 10% co-solvent. The extraction yield of pigments was also closely related to moisture content of the raw material. Extraction yield of safflower yellow pigments by SCF extraction at optimized conditions was 6% higher than that by solvent extraction. Supercritical carbon dioxide was proved to be suitable for the extraction of safflower yellow pigments from Carthamus tinctorius L.

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Chromosome Aberration Test of Water Extract of Decaffeined Green Tea using Supercritical Carbon Dioxide With Mammalian Cell Line (초임계이산화탄소를 이용한 탈카페인녹차 열수추출물의 포유동물 세포주를 이용한 염색체이상시험)

  • Koo, Yun-Chang;Lee, Hyun-Sun;Park, Byung-Gyu;Kim, Eun-Jin;Lee, Sun-Joo;Kim, Kyoung-Hoen;Kim, Young-Suk;Kim, Kwang-Ok;Chung, Young-Shin;Lee, Kwang-Won
    • Environmental Mutagens and Carcinogens
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    • v.26 no.3
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    • pp.63-68
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    • 2006
  • There are $10{\sim}30%$ polyphenol and $2{\sim}4%$ caffeine in green tea. Caffeine is a kind of alkaloid containing nitrogen which cause stimulation, impatience, headache, insomnia, low birth weight infant. Because of these negative effect, decaffeined beverage came out and decaffeined coffee already have a big market since 1970s. Having proving the physiologic functions of green tea, high consumption of coffee is shifting to green tea. Because of the carcinogenic effect of the organic solvents, decaffeine processing with supercritical carbon dioxide has industrialized and have an advantage in environment-friendly and minimized flavor loss. Decaffeined green tea using supercritical carbon dioxide is considered to be safe but there are not enough study, We investigated the chromosome aberration test with mammalian cell line, CHL. When the cells were treated with 5000, 2000, 1000 ${\mu}g/ml$ and compared with the negative controls, there were no significant (P>0.05) increased chromosome aberration. Same results was observed when adding S9 mixture or not. As a result, water extract of decaffeined green tea using supercritical carbon dioxide does not induce chromosome aberration.

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Chromosome Aberration Test of Water Extract of Decaffeined Green Tea using Supercritical Carbon Dioxide with Mammalian cell line (초임계이산화탄소를 이용한 탈카페인녹차 열수추출물의 포유동물 세포주를 이용한 염색체이상시험)

  • Koo, Yun-Chang;Lee, Hyun-Sun;Park, Byung-Gyu;Kim, Eun-Jin;Lee, Sun-Joo;Kim, Kyoung-Hoen;Kim, Young-Suk;Chung, Young-Shin;Lee, Kwang-Won
    • Environmental Mutagens and Carcinogens
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    • v.26 no.4
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    • pp.119-124
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    • 2006
  • There are $10{\sim}30%$ polyphenol and $2{\sim}4%$ caffeine in green tea. Caffeine is a kind of alkaloid containing nitrogen which cause stimulation, impatience, headache, insomnia, low birth weight infant. Because of these negative effect, decaffeined beverage came out and decaffeined coffee already have a big market since 1970s. Having proving the physiologic functions of green tea, high consumption of coffee is shifting to green tea. Because of the carcinogenic effect of the organic solvents, decaffeine processing with supercritical carbon dioxide has industrialized and have an advantage in environment-friendly and minimized flavor loss. Decaffeined green tea using supercritical carbon dioxide is considered to be safe but there are not enough study. We investigated the chromosome aberration test with mammalian cell line, CHL. When the cells were treated with 5000, 2000, 1000 ${\mu}g/ml$ and compared with the negative controls, there were no significant(P>0.05) increased chromosome aberration. Same results was observed when adding S9 mixture or not. As a result, water extract of decaffeined green tea using supercritical carbon dioxide does not induce chromosome aberration.

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Extraction and Identification of Volatile Isothiocyanates from Wasabi using Supercritical Carbon Dioxide (초임계 이산화탄소를 이용한 고추냉이로부터 휘발성 Isothiocyanates류 추출 및 동정)

  • Kim, Sung-Jin;Lee, Min-Kyung;Back, Sung-Sin;Chun, Byung-Soo
    • KSBB Journal
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    • v.22 no.3
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    • pp.174-178
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    • 2007
  • The aim of this study was to identifyisothiocyanates (ITCs) from wasabi (Wasabi japonica Matsum) using supercritical carbon dioxide ($SCO_2$) and to compare the composition in the extracts between $SCO_2$ and organic solvents extraction. A semi-continuous high pressure apparatus was used to extract wasabi (roots, stems and leaves) at following conditions pressure 80$\sim$120 bar, temperature $40\sim50^{\circ}C$. Ether, ethanol, chloroform and dichloromethane were used as organic solvents. The ITCs extracted by means of both separation technologies were analyzed by a gas chromatography system. As the results of study, AITC and ITCs were highly extracted at 40$^{\circ}C$ and 80 bar. To extract AITC from wasabi, $SCO_2$ extraction is more effective than organic solvents extraction, resulted in thermal degeneration and remaining of organic solvents.

Measurement of Antioxidant Activities and Phenolic and Flavonoid Contents of the Brown Seaweed Sargassum horneri: Comparison of Supercritical CO2 and Various Solvent Extractions

  • Yin, Shipeng;Woo, Hee-Chul;Choi, Jae-Hyung;Park, Yong-Beom;Chun, Byung-Soo
    • Fisheries and Aquatic Sciences
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    • v.18 no.2
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    • pp.123-130
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    • 2015
  • Seaweed Sargassum horneri extracts were obtained using supercritical carbon dioxide ($SC-CO_2$) and different solvents. $SC-CO_2$ was kept at a temperature of $45^{\circ}C$ and pressure of 250 bar. The flow rate of $CO_2$ (27 g/min) was constant during the entire 2-h extraction period, and ethanol was used as a cosolvent. Six different solvents [acetone, hexane, methanol, ethanol, acetone mix methanol (7:3), and hexane mix ethanol (9:1)] were used for extraction and agitated by magnetic stirring (250 rpm) in the dark at $25^{\circ}C$ for 20 h; the ratio of material to solvent was 1:10 (w/v). Antioxidant properties of S. horneri extracted using $SC-CO_2$ with ethanol and different solvents have shown good activity. The highest activity belongs to $SC-CO_2$ with ethanol extracted oil, showing DPPH, ABTS, total phenolic content, and total flavonoid levels of $68.38{\pm}1.21%$, $83.51{\pm}1.25%$, $0.64{\pm}0.02mg/g$, and $5.57{\pm}0.05mg/g$, respectively. The S. horneri extracts showed a significant correlation between the antioxidant activity and phenolic content. Based on these results, the $SC-CO_2$ extract (ethanol) of the seaweed extract from brown seaweed may be a valuable antioxidant source.

Effect of Kamichungbieum on Allergic Reaction (가미청비음이 알러지반응에 미치는 영향)

  • Kim Kwang Sik;Lee Dong Hee;Kwon Young An;choi Suh Young;Eun Jae Soon
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.18 no.3
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    • pp.857-862
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    • 2004
  • The purpose of this research was to investigate the effects of supercritical fluid extract of Kamichungbieum (SFE) on allergic reaction. SFE (500 mg/kg) inhibited the systemic anaphylaxis induced by compound 48/80 or platelet activating factor and inhibited the passive cutaneous anaphylaxis (PCA) induced by anti-dinitrophenyl (DNP)-lgE and DNP-human serum albumin (HSA) in vivo. Also, SFE inhibited the SRSC-induced delayed type hypersensitivity and inhibited the hind paw edema induced by histamine. In addition, SFE inhibited the permeability of evans blue induced by acetic acid and inhibited the writhing syndrome induced by acetic acid. These results indicate that SFE may be useful for the prevention and treatment of allergy related disease.