• 한국신재생에너지학회:학술대회논문집
• /
• 2010.06a
• /
• pp.167.1-167.1
• /
• 2010

본 연구에서는 수직 밀폐형 지중열교환기 뒤채움용 그라우트의 종류와 첨가재 종류, 지중열교환기 파이프 단면에 따른 지중열교환기의 성능을 비교 평가하기 위해 현장 시험 시공과 현장 열응답 시험을 수행하였다. 뒤채움용 그라우트재는 벤토나이트와 시멘트를 사용하였으며 첨가제로는 천연규사와 흑연을 적용하였다. 지중열교환기 파이프 단면은 일반적으로 시공되는 U-loop 파이프 단면과 파이프 사이의 열간섭 효과를 최소화 한 3공형 파이프 단면이 적용되었다. 시멘트-천연규사 그라우트재가 벤토나이트-천연규사 그라우트재 보다 큰 지중 유효열전도도를 보이고 흑연을 첨가한 그라우트는 시멘트와 벤토나이트 모두에서 천연규사만 첨가하였을 때 보다 지중 유효열전도도가 높게 나타났다. 3공형 파이프 단면의 경우 단면에 따른 영향을 비교하기 위해 그라우트는 시멘트-천연규사와 벤토나이트-천연규사를 사용하였으며 지중 유효열전도도 측정결과 각각 3.64 W/mK, 3.40 W/mK으로 일반 U-loop 파이프 단면을 사용하였을 때 보다 높게 나타났다.

• Molecules and Cells
• /
• v.39 no.11
• /
• pp.783-789
• /
• 2016

Afflicted neurons in various neurodegenerative diseases generally display diverse and complex pathological features before catastrophic occurrence of massive neuronal loss at the late stages of the diseases. This complex nature of neuronal pathophysiology inevitably implicates systemwide changes in basic cellular activities such as transcriptional controls and signal cascades, and so on, as a cause. Recently, as one of these systemwide cellular changes associated with neurodegenerative diseases, epigenetic changes caused by protein toxicity have begun to be highlighted. Notably, recent advances in related techniques including next-generation sequencing (NGS) and mass spectrometry enable us to monitor changes in the post-translational modifications (PTMs) of histone proteins and to link these changes in histone PTMs to the specific transcriptional changes. Indeed, epigenetic alterations and consequent changes in neuronal transcriptome are now begun to be extensively studied in neurodegenerative diseases including Alzheimer's disease (AD). In this review, we will discuss details of our current understandings on epigenetic changes associated with two representative neurodegenerative diseases [AD and polyglutamine (polyQ) diseases] and further discuss possible future development of pharmaceutical treatment of the diseases through modulating these epigenetic changes.

• BMB Reports
• /
• v.48 no.1
• /
• pp.25-29
• /
• 2015

Ubiquitination is a post translational modification which mostly links with proteasome dependent protein degradation. This process has been known to play pivotal roles in the number of biological events including apoptosis, cell signaling, transcription and translation. Although the process of ubiquitination has been studied extensively, the mechanism of polyubiquitination by multi protein E3 ubiquitin ligase, SCF complex remains elusive. In the present study, we identified UbcH5a as a novel stimulating factor for poly-ubiquitination catalyzed by $SCF^{hFBH1}$ using biochemical fractionations and MALDI-TOF. Moreover, we showed that recombinant UbcH5a and Cdc34 synergistically stimulate $SCF^{hFBH1}$ catalyzed polyubiquitination in vitro. These data may provide an important cue to understand the mechanism how the SCF complex efficiently polyubiquitinates target substrates.

• 한국신재생에너지학회:학술대회논문집
• /
• 2011.05a
• /
• pp.196.1-196.1
• /
• 2011

In this study, a test bed was constructed in order to evaluate thermal efficiency of the energy pile which carries out combined roles of a structural foundation and of a heat exchanger. The energy pile in this study is designed as a large-diameter drilled shaft equipped with the heat exchange pipes which configures a W-shape and an S-shape. The drilled shaft reached to the depth of 60 m whilst the heat exchange pipes were installed to about 30 m deep from the ground surface. The W-shaped and S-shaped heat exchange pipes were installed in the opposite sections of the same drilled shaft. In-situ thermal response tests were performed for both the shapes of heat exchange pipes. To avoid underestimating the thermal performance due to hydration heat of concrete inside the drilled shaft, the in-situ thermal response tests for the energy pile were performed after four weeks since the installation of the energy pile.

• Horticultural Science & Technology
• /
• v.30 no.6
• /
• pp.757-763
• /
• 2012

To acquaint correct information about the fertilizability and analyze S-allele based genetic diversity among Korean apples, we investigated self-incompatibility genotypes by PCR and cross-pollination tests in field. As a consequence, S-genotypes of Korean apples were distributed within narrow genetic diversity as $S_1S_3$ for 'Hongro' and 'Saenara'; $S_1S_9$ for 'Gamhong' and 'Manbok'; $S_3S_5$ for 'Seokwang'; $S_3S_9$ for 'Sunhong', 'Seohong', 'Chukwang', and 'Hwahong'. Coupled with cross-pollination experiments in field, our results provide support for the view that apples are fully compatible when both of their S-loci differ and semi-compatible when they carry one different and one identical S-locus. Furthermore, the results of this study indicate that S-alleles have to be extended to various genotypes for Korean apple breeding.

• Journal of Microbiology and Biotechnology
• /
• v.24 no.5
• /
• pp.714-718
• /
• 2014

Apoptosis is the process of programmed cell death executed by specific proteases, the caspases, which mediate the cleavage of various vital proteins. Elucidating the consequences of this endoproteolytic cleavage is crucial to understanding cell death and other related biological processes. Although a number of possible roles for caspase-6 have been proposed, the identities and functions of proteins that interact with caspase-6 remain uncertain. In this study, we established a cell line expressing tandem affinity purification (TAP)-tagged caspase- 6 and then used LC-MS/MS proteomic analysis to analyze the caspase-6 interactome. Eight candidate caspase-6-interacting proteins were identified. Of these, five proteins (hnRNP-M, DHX38, ASPP2, MTA2, and UACA) were subsequently examined by co-immunoprecipitation for interactions with caspase-6. Thus, we identified two novel members of the caspase-6 interactome: hnRNP-M and MTA2.

• Journal of Microbiology and Biotechnology
• /
• v.25 no.9
• /
• pp.1578-1582
• /
• 2015

Granzyme A (GzmA) was identified as a cytotoxic T lymphocyte protease protein expressed in the nucleus. A number of nuclear proteins are well known as GzmA substrates, and GzmA is related with caspase-independent apoptosis. Histones H1, H2B, and H3 were identified as GzmA substrates through in vitro experiment with purified nucleosome. Here, we demonstrated that histone H3 was cleaved by GzmA in vivo during staurosporine-induced cell death. Moreover, histone H3 cleavage was blocked by the GzmA inhibitor nafamostat mesylate and by GzmA knockdown using siRNA. Taken together, we verified that histone H3 is a real substrate for GzmA in vivo in the Raji cells treated by staurosporin.

• BMB Reports
• /
• v.49 no.10
• /
• pp.560-565
• /
• 2016

Granzyme A (GzmA) was first identified as a cytotoxic T lymphocyte protease protein with limited tissue expression. A number of cellular proteins are known to be cleaved by GzmA, and its function is to induce apoptosis. Histones H1, H2B, and H3 were identified as GzmA substrates during apoptotic cell death. Here, we demonstrated that histone H4 was cleaved by GzmA during staurosporine-induced cell death; however, in the presence of caspase inhibitors, staurosporine-treated Raji cells underwent necroptosis instead of apoptosis. Furthermore, histone H4 cleavage was blocked by the GzmA inhibitor nafamostat mesylate and by GzmA knockdown using siRNA. These results suggest that histone H4 is a novel substrate for GzmA in staurosporine-induced cells.

• BMB Reports
• /
• v.53 no.7
• /
• pp.373-378
• /
• 2020

Phosphorylation of the signaling component by protein kinase often leads to a kinase cascade or feedback loop. 3-Phosphoinositide-dependent kinase 1 (PDK1) signaling pathway diverges into various kinases including Akt and p70 S6 kinase (p70S6k). However, the PDK1 feedback mechanism remains elusive. Here, we demonstrated that UNC-51-like kinase (ULK1), an autophagy initiator kinase downstream of mechanistic target of rapamycin (mTOR), directly phosphorylated PDK1 on serine 389 at the linker region. Furthermore, our data showed that this phosphorylation affected the kinase activity of PDK1 toward downstream substrates. These results suggest a possible negative feedback loop between PDK1 and ULK1.

• BMB Reports
• /
• v.47 no.3
• /
• pp.173-178
• /
• 2014

There is rapidly growing interest in the human microbiome because of its implication in metabolic disorders and inflammatory diseases. Consequently, understanding the biology of short chain fatty acids and their receptors has become very important for identifying novel therapeutic avenues. GPR41 and GPR43 have been recognized as the cognate receptors for SCFAs and their roles in metabolism and inflammation have drawn much attention in recent years. GPR43 is highly expressed on immune cells and has been suggested to play a role in inflammatory diseases such as inflammatory bowel disease. Both GPR41 and GPR43 have been implicated in diabetes and obesity via the regulation of adipose tissue and gastrointestinal hormones. So far, many studies have provided contradictory results, and therefore further research is required to validate these receptors as drug targets. We will also discuss the synthetic modulators of GPR41 and GPR43 that are critical to understanding the functions of these receptors.