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FITNESS OF THE COLLARLESS METAL-CERAMIC RESTORATIONS AT LABIAL MARGINS: A SCANNING ELECTRON MICROSCOPE STUDY (도재전장주조관의 순측 치경부 변연 적합도에 관한 주사전자현미경적 연구)

  • Kim, Young-Han;Lee, Sun-Hyung
    • The Journal of Korean Academy of Prosthodontics
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    • v.23 no.1
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    • pp.113-123
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    • 1985
  • The purpose of this study was to evaluate the marginal fitness of collarless metal-ceramic crown formed with direct lift technique using wax compared with metal-ceramic crown with metal butt margin. Twenty crowns, ten were collarless metal-ceramic crowns and ten were metal-ceramic crowns with metal butt margins, were made and cemented on epoxy resin dies, and their marginal openings were measured on scanning electron microphotographic prints at x200 magnification. The results were as follows; 1. In the case of collarless metal-ceramic crowns, the mean marginal opeining was $43.78{\pm}17.67{\mu}m$, the mean maximum marginal opening was $52.81{\pm}19.81{\mu}m$, and the mean minimum marginal opening was $34.99{\pm}16.58{\mu}m$. 2. In the case of metal-ceramic crowns with metal butt margins, the mean marginal opening was $47.62{\pm}25.55{\mu}m$, the mean maximum marginal opening was $61.90{\pm}24.39{\mu}m$, and the mean minimum marginal opening was $34.86{\pm}24.92{\mu}m$. 3. There was no significant difference between two types of crowns at the 99% confidence level. 4. The marginal fitness of collarless metal-ceramic crown formed with direct lift technique using wax was clinically acceptable.

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Incidence and Level of Aflatoxins Contamination in Medicinal Plants in Korea

  • Lee, Sung Deuk;Yu, In Sil;Jung, Kweon;Kim, Yeon Sun
    • Mycobiology
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    • v.42 no.4
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    • pp.339-345
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    • 2014
  • During 2011~2013, a total of 729 samples for 19 types of medicinal plant were collected from Seoulyekryungsi in Seoul, Korea, and investigated for the presence of aflatoxins. The samples were analyzed using immunoaffinity column cleanup and high-performance liquid chromatography coupled to a fluorescence detector after post-column derivatization. Aflatoxins were found in 124 out of the 729 analyzed samples: 65 containing aflatoxin B1 (AFB1), 24 with aflatoxin B2 (AFB2), 15 with aflatoxin G1 (AFG1), and 20 samples with aflatoxin G2 (AFG2). The ranges for positive samples were $0.1{\sim}404.7{\mu}g/kg$ for AFB1, $0.1{\sim}10.0{\mu}g/kg$ for AFB2, $0.1{\sim}635.3{\mu}g/kg$ for AFG1, $0.1{\sim}182.5{\mu}g/kg$ for AFG2, and $0.1{\sim}1,043.9{\mu}g/kg$ for total aflatoxins. Most of the medicinal plant samples (721, 98.9%) were below legal limits, but 8 samples exceeded the legal limits of 10 and $15{\mu}g/kg$ established by the Korean standard for AFB1 and total aflatoxins (the sum of AFB1, AFB2, AFG1 and AFG2), respectively.

Lipoxygenase Inhibitory and Antioxidant Activities of Isolated Compounds from Moutan Cortex

  • Ha, Do-Thi;Trung, Trinh-Nam;Thuan, Nguyen-Duy;Yim, Nam-Hui;Min, Byung-Sun;Bae, Ki-Hwan
    • Natural Product Sciences
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    • v.16 no.2
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    • pp.68-74
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    • 2010
  • Phytochemical investigation on the ethyl acetate and n-butanol fractions of Moutan Cortex resulted in the isolation and characterization of a new monoterpene glycoside (3) and twenty known monoterpene glycosides (1, 2, 4-21). The structure of 3 was determined by spectroscopic data interpretation and physico-chemical properties. Compounds 1 and 8 presented a remarkable inhibitory activity against lipoxygenase-1 (LOX-1) with $IC_{50}$ values of 45.2 and $37.5\;{\mu}M$, respectively. Compounds 9, 10, 13, 18, 19, and 21 showed significant 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging effect with $IC_{50}$ values of 9.8, 25.5, 6.4, 15.2, 18.7, and $23.7\;{\mu}M$, respectively. Benzoylpaeoniflorin (8), which exhibited the highest inhibitory effect with an $IC_{50}$ value of $37.5{\pm}0.7{\mu}M$, was further analyzed the inhibition kinetics by Lineweaver-Burk plots. Results indicated that 8 is a non-competitive inhibitor, and the kinetic parameter values were estimated to be ($31.04\;{\mu}M$, Ki), ($0.29\;{\mu}M/min$, $V_m$), and ($48.50\;{\mu}M$, $K_m$).

Improvements in Solar Cell Efficiency using a PMMA Concentrator Lens for Indoor Use (실내조명 응용을 위한 투명 집광 렌즈를 이용한 태양전지 효율 향상)

  • Lee, Yoo-Jong
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.14 no.4
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    • pp.929-934
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    • 2010
  • Improvements in characteristics of a single junction AlGaAs/GaAs solar cell are observed by capping a PMMA lens on it. In order to show the effect of the lens, characteristics of a single junction AlGaAs/GaAs solar cell before and after the lens formation are compared under the one-sun illumination condition ($100mW/cm^2$). Characteristics of the solar cell under very weak illumination condition (about 1200 lux) is also measured with the lighting of a fluorescent desk lamp. About 5% of cell efficiency is improved after the capping of PMMA lens on the single junction AlGaAs solar cell and $83\;{\mu}m/cm^2$ of electrical power was generated with the lighting of a desk lamp.

Life Styles and the Blood Cadmium Concentration in Korean Adults (한국 성인에서의 생활행태와 혈중 카드뮴 농도)

  • Lee, Sang-Hyuk;Choi, Byung-Sun;Park, Jung-Duck
    • Environmental Analysis Health and Toxicology
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    • v.24 no.3
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    • pp.193-202
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    • 2009
  • In this study, we analyzed the blood cadmium concentration in general population of Korea, and followed the analyses the relations with epidemiologic factors, life-style and body iron store. The geometric mean concentration of blood cadmium was 1.20 ${\mu}g/L$ in study subject, the level of blood cadmium was higher in female (1.27 ${\mu}g/L$) than in male (1.11 ${\mu}g/L$). The mean concentration of blood cadmium was the highest in coastal area (1.80 ${\mu}g/L$) and followed in urban (1.01 ${\mu}g/L$) and rural area (0.90 ${\mu}g/L$). The cadmium concentration in whole blood was increased with age-dependent pattern, such as 0.64 ${\mu}g/L$ in the below 30 years, 0.95 ${\mu}g/L$ in the 30~44 years, 1.28 ${\mu}g/L$ in the 45~59 years and 1.31 ${\mu}g/L$ in the over 60 years. The level of blood cadmium was higher in smokers than in non-smokers, but was not significant by alcoholic habit. The blood cadmium level was different from occupations, which was the highest in the fishers as 1.87 ${\mu}g/L$. The inversed relation was observed between blood cadmium and serum Fe, while was correlated positively with the ferritin level. In summary, the environmental exposure to cadmium in Korean is not so much compared to other countries, and the blood cadmium is influenced by genetic factors (age and sex), life-styles (dietary habit, occupation and smoking habit) and nutrition such as serum iron.

Effects of Benzyl Isothiocyanate and Its N-Acetylcysteine Conjugate on Induction of Detoxification Enzymes in Hepa1c1c7 Mouse Hepatoma Cells

  • Hwang, Eun-Sun
    • Preventive Nutrition and Food Science
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    • v.19 no.4
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    • pp.268-273
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    • 2014
  • The induction of detoxification enzymes by benzyl isothiocyanate (BITC) and its synthetic N-acetyl-L-cysteine (NAC) conjugate (NAC-BITC) was examined in Hepa1c1c7 murine hepatoma cells. BITC and NAC-BITC inhibited Hepa1c1c7 cell growth in a dose-dependent manner. Cell growth was 4.5~57.2% lower in Hepa1c1c7 cells treated with $0.1{\sim}1.0{\mu}M$ BITC than in control-treated Hepa1c1c7 cells. The NAC-BITC treatment had a similar inhibitory pattern on Hepa1c1c7 cell growth; $0.5{\mu}M$ and $10{\mu}M$ NAC-BITC decreased cell growth by 13.6% and 47.4%, respectively. Treatment of Hepa1c1c7 cells with $0.1{\sim}2.0{\mu}M$ BITC also elicited a dose-response effect on the induction of quinone reductase quinone reductase (QR) activity and QR mRNA expression. Treatment with $1{\mu}M$ and $2{\mu}M$ BITC caused 1.8- and 2.8-fold inductions of QR mRNA, respectively. By comparison, treatment with $1{\mu}M$ and $2{\mu}M$ NAC-BITC caused 1.6-and 1.9-fold inductions of QR mRNA, respectively. Cytochrome P450 (CYP) 1A1 and CYP2E1 induction were lower in $0.1{\sim}2{\mu}M$ BITC-treated cells than in control-treated cells. CYP2E1 activity was 1.2-fold greater in $0.1{\mu}M$ NAC-BITC-treated cells than in control-treated cells. However, the CYP2E1 activity of cells treated with higher concentrations (i.e., $1{\sim}2{\mu}M$) of NAC-BITC was similar to the activity of control-treated cells. Considering the potential of isothiocyanatesto prevent cancer, these results provide support for the use of BITC and NAC-BITC conjugates as chemopreventive agents.

Implementation of RF Frequency Synthesizer for IEEE 802.15.4g SUN System (IEEE 802.15.4g SUN 시스템용 RF 주파수 합성기의 구현)

  • Kim, Dong-Shik;Yoon, Won-Sang;Chai, Sang-Hoon;Kang, Ho-Yong
    • Journal of the Institute of Electronics and Information Engineers
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    • v.53 no.12
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    • pp.57-63
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    • 2016
  • This paper describes implementation of the RF frequency synthesizer with $0.18{\mu}m$ silicon CMOS technology being used as an application of the IEEE802.15.4g SUN sensor node transceiver modules. Design of the each module like VCO, prescaler, 1/N divider, ${\Delta}-{\Sigma}$ modulator, and common circuits of the PLL has been optimized to obtain high speed and low noise performance. Especially, the VCO has been designed with NP core structure and 13 steps cap-bank to get high speed, low noise, and wide band tuning range. The output frequencies of the implemented synthesizer is 1483MHz~2017MHz, the phase noise of the synthesizer is -98.63dBc/Hz at 100KHz offset and -122.05dBc/Hz at 1MHz offset.

The Ecophysiological Characteristics of Acanthopanax senticosus of Leaves(I) -Net Photosynthetic Rates, Stomatal Transpirations, Stomatal Conductances of Leaves- (가시오갈피의 생리·생태적 특성(I) -산지별 순광합성, 기공증산, 기공전도도-)

  • Han, Sang-Sup;Kwon, Jeong-Jung;Lee, Kab-Yeon;Hur, Sung-Du;Kim, Ha-Sun;Kim, Jong-Won
    • Journal of Forest and Environmental Science
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    • v.19 no.1
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    • pp.18-24
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    • 2003
  • This study was made on the physiological reactions of photosynthesis. stomatal transpiration. stomatal conductance of Acanthopanax senticosus of leaves. The results obtained are as follows 1. The light compensation points were approximately $30{\mu}molm^{-2}S^{-1}$ in sun leaves and $15{\mu}molm^{-2}S^{-1}$ in shade leaves. 2. The light saturation points were approximately $1,000{\mu}molm^{-2}S^{-1}$ in sun leaves and $300{\mu}molm^{-2}S^{-1}$ in shade lea ves. 3. There was no significant between various mountains distributed. net photosynthetic rates were approximately 8.0 to $8.8{\mu}molm^{-2}S^{-1}$ in upper leaves. However. net photosynthetic rate in upper leaves of Acanthopanax koreanum in Jeju island was slightly low about $6.9{\mu}molm^{-2}S^{-1}$. 4. Net photosynthetic rate in middle leaves of all mountains was ranged from 40% to 65% of upper leaves. and that in lower leaves was approximately 30% of upper leaves. But the net photosynthesis in lower leaves of Acanthopanax koreanum in Jeju island was slightly high about 71 % of upper leaves. 5. There was no significant between various mountains distributed, stomatal transpirations were ranged from 1.1 to $1.4mmolH_2Om^{-2}S^{-1}$ in upper leaves, 0.7 to $1.0mmolH_2Om^{-2}S^{-1}$ in middle leaves, and 0.5 to $0.6mmolH_2Om^{-2}S^{-1}$ in lower leaves. respectively. 6. There was no significant between various mountains distributed. the stomatal conductance in upper 1eaves was ranged from 70 to $90mmolH_2Om^{-2}S^{-1}$. However, stomatal conductance in upper leaves of Acanthopanax koreanum in Jeju island was approximately $380mmolH_2Om^{-2}S^{-1}$, its remarkedly higher than any other mountain.

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Acute and Chronic Toxicity of heavy Metals to Daphnia magna (물벼룩(Daphnia magna)에 대한 중금속의 급성 및 만성 독성)

  • Jeong, Jae-Won;Cha, Mi-Seon;Jo, Sun-Ja;Lee, Sang-Jun
    • Journal of Environmental Science International
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    • v.10 no.4
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    • pp.293-298
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    • 2001
  • The toxicity values heavy metals were evaluated by immobilization and chronic reproduction impairment tests, using Daphnia magna. Acute tests were evaluated by the inhibition of their mobilization after 24hrs without food addition. The tests of reproductive impairment were investigated for 21 days by food addition and exchange or water. The effect of each concentration was assessed by Probit analysis and t-test. The results obtained from this study were as follows : 1) The change of pH and DO was not significant in the acute tests, while, in the reproductive tests, pH was increased by 0.3~1.4 and DO also increased. 2) The $E_iC_{50}$ values of immobilization to Daphnia magna in artificial fresh water were $0.030mg/{\ell}(Cu),\;0.054mg/{\ell}(Cd),\;0.12mg/{\ell};(Cr),\;0.74mg/{\ell}(Pb),\;3.4mg/ {\ell}(As)$ and the $NOE_iC$ values were $0.010mg/{\ell}(Cu),\;0.018mg/{\ell}(Cd),\;0.010mg/{\ell}(Cr),\;0.10mg/{\ell}(Pb),\;and\;$1.8mg/{\ell}(As)$. 3) The $E_rC_{50}$ values of reproductive impairment to Daphnia magna were $13.8\mu\textrm{g}/{\ell}(Cu),\;2.9\mu\textrm{g}/{\ell}(Cd),\;15.5\mu\textrm{g}/{\ell}(Cr),\;61.7\mu\textrm{g}/{\ell}(Pb),\;759\mu\textrm{g}/{\ell}(As)$, and $NOE_rC$ values were $0.95\mu\textrm{g}/{\ell}(Cu),\;$0.54\mu\textrm{g}/{\ell}(Cd),\;1.2\mu\textrm{g}/{\ell}(Cd),\;$7.4\mu\textrm{g}/{\ell}(Pb),\;110mu\textrm{g}/{\ell}(As)$. The results of tests using OECD artificial culture water were more sensitive than natural water for culturing. The presented data show that an artificial culture water is suitable in the experiment of bioassay for assessing the toxicity of marterials.

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Cadmium resistance in tobacco plants expressing the MuSI gene

  • Kim, Young-Nam;Kim, Ji-Seoung;Seo, Sang-Gyu;Lee, Young-Woo;Baek, Seung-Woo;Kim, Il-Sup;Yoon, Ho-Sung;Kim, Kwon-Rae;Kim, Sun-Hyung;Kim, Kye-Hoon
    • Plant Biotechnology Reports
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    • v.5 no.4
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    • pp.323-329
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    • 2011
  • MuSI, a gene that corresponds to a domain that contains the rubber elongation factor (REF), is highly homologous to many stress-related proteins in plants. Since MuSI is up-regulated in the roots of plants treated with cadmium or copper, the involvement of MuSI in cadmium tolerance was investigated in this study. Escherichia coli cells overexpressing MuSI were more resistant to Cd than wild-type cells transfected with vector alone. MuSI transgenic plants were also more resistant to Cd. MuSI transgenic tobacco plants absorbed less Cd than wild-type plants. Cd translocation from roots to shoots was reduced in the transgenic plants, thereby avoiding Cd toxicity. The number of short trichomes in the leaves of wild-type tobacco plants was increased by Cd treatment, while this was unchanged in MuSI transgenic tobacco. These results suggest that MuSI transgenic tobacco plants have enhanced tolerance to Cd via reduced Cd uptake and/or increased Cd immobilization in the roots, resulting in less Cd translocation to the shoots.