• Asian-Australasian Journal of Animal Sciences
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• 제20권7호
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• pp.1049-1056
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• 2007

This study was conducted to compare the methane ($CH_4$) production estimated by in vivo (sulfur hexafluoride tracer technique ($SF_6$)) with that of two in vitro rumen simulation (RUSITEC) and gas production (IVGPT)) techniques. Four adult dry Holstein cows, aged $7.4{\pm}3.0$ years and weighing $697{\pm}70$ kg, were used for measuring methane production from five diets by the $SF_6$ technique. The experimental diets were alfalfa hay ($D_1$), corn silage + soybean meal (SBM) (910: 90, $D_2$), Italian rye grass hay +SBM (920: 80, $D_3$), rice straw +SBM (910: 90, $D_4$) and Sudan grass hay +SBM (920: 80, $D_5$). Each diet was individually fed to all 4 cows and 5 feeding studies of 17 d each were conducted to measure the methane production. In the RUSITEC, methane production was measured from triplicate vessels for each diet .In vitro gas production was measured for each of the diets in triplicate syringes. The gas produced after 24 and 48 h was recorded and gas samples were collected in vacuum vials and the methane production was calculated after correction for standard temperature and pressure (STP). Compared to the $SF_6$ technique, estimates of methane production using the RUSITEC were lower for all diets. Methane production estimated from 24 h in vitro gas production was higher (p<0.001) on $D_1$ as compared to that measured by $SF_6$, whereas on $D_2$ to $D_5$ it was lower. Compared to $SF_6$, methane production estimated from 48 h in vitro gas production was higher on all diets. However, methane estimated from the mean of the two measurement intervals (24+48 h/2) in IVGPT was very close to that of $SF_6$ (correlation 0.98), except on $D_1$. The results of our study confirmed that IVGPT is reflective of in vivo conditions, so that it could be used to generate a database on methane production potential of various ruminant diets and to examine strategies to modify methane emissions by ruminants.

• 한국초지조사료학회지
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• 제36권2호
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• pp.98-103
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• 2016

전라북도 고창 조사 지역 내 유기 낙농 농가 수는 연도가 지남에 따라 증가하지 않았지만, 전체적인 사육규모와 사육두수는 증가하였다. 연 평균 유량은 국내 검정 농가 평균에 비해 유기 낙농가가 최대 9 kg/d가 낮았지만 유지방과 유단백 함량에서는 큰 차이를 보이지 않았다. NRC (2001)의 사양표준 프로그램을 이용하여 영양소 공급 수준을 평가한 결과, 급여 사료의 에너지 비율 특히 비구조탄수화물의 비율을 감소시킬 필요가 있으며, 또한 대사단백질 및 섬유소 비율을 증가시켜 전체적인 영양소 공급 수준을 조정한다면 착유우의 건강상태 및 유생산성이 개선될 것으로 생각된다.

• Asian-Australasian Journal of Animal Sciences
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• 제21권5호
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• pp.663-670
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• 2008

The effect of supplementing the diet of grazing dairy cows with fish oil (FO) and linseed oil (LSO) on milk conjugated linoleic acid (CLA) was investigated. Sixteen Holstein cows (17019 DIM) were assigned into two groups and fed a grain supplement (8.0 kg/d; DM basis) containing 800 g of saturated animal fat (CONT) or 200 g FO and 600 g LSO (FOLSO). All cows grazed together on Sudan grass pasture ad libitum and were fed the treatment diets for 3 wks. Cows were milked twice a day and milk samples were collected during the last three days of the trial. Milk production (24.89 and 22.45 kg/d), milk protein percentage (2.76 and 2.82) and milk protein yield (0.68 and 0.64 kg/d) for the CONT and FOLSO diets, respectively, were not affected (p>0.05) by treatment diets. Milk fat percentage (3.90 and 2.86) and milk fat yield (0.97 and 0.64 kg/d) were lower (p<0.05) with the FOLSO diet compared with the CONT diet. The concentration and yield of milk cis-9 trans-11 CLA were higher (p<0.05) with the FOLSO diet (2.56% of total FA and 16.44 g/d, respectively) than the CONT diet (0.66% of total FA and 6.44 g/d, respectively). The concentrations of milk trans C18:1 and vaccenic acid (VA) were higher (p<0.05) with the FOLSO diet (13.53 and 7.48% of total FA, respectively) than the CONT diet (3.69 and 2.27% of total FA, respectively). In conclusion, supplementing the diet of grazing cows with FO and LSO increased milk cis-9 trans-11 CLA content but reduced milk fat content and yield.

• Asian-Australasian Journal of Animal Sciences
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• 제11권2호
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• pp.155-161
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• 1998

This study conducted to evaluate effects of popped soybean on levels of ruminal peptides and blood amino acids in Holstein calves fed sudan grass hay as a forage source and popped (PSB) soybean as a concentrate supplement. At 0, 2, 4 and 6 h after feeding, rumen fluid and blood samples were collected from the rumen and jugular vein, respectively, and amino acids, peptides and other nitrogen-containing compounds in the rumen were analyzed. Ruminal pH tended to be higher in the RSB than in the PSB treatments, and declined upto 4 h after feeding, since then increased in both treatments. The concentrations of ammonia-N in all treatments increased upto 2 h after feeding, and then decreased gradually with time after feeding. The concentrations of ammonia N in the rumen were not significantly different between the treatments, however, those in RSB treatment appeared to be higher. Also, protein concentrations in the rumen were not significantly different between the treatments. Peptide productions were the highest at 2 h after feeding in the group fed RSB which is rapidly degradable in rumen, whereas those in the group fed PSB which is slowly degradable in rumen were maximized at 4 h after feeding. The concentration of total free essential amino acids in plasma was higher in the RSB treatment than in the PSB, but disappearance rates of these amino acids out of plasma was higher in the PSB treatment than in the RSB treatment. Disappearance rates of free non-essential amino acids in plasma were not significantly different between the treatments. Consequently, this study implies that the production of peptide and utilization of blood amino acid may be controlled by the modification of protein degradability.

• 한국토양비료학회지
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• 제45권1호
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• pp.51-58
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• 2012

Salinity is one of the most relevant abiotic factor limiting crop yield and its net primary productivity. In addition, salinity induces an increased stress ethylene synthesis in plants which, in turn, exacerbate the responses to the stressor. Bacterial single or co-inoculation effect was tested using previously characterized plant growth promoting (PGP) bacteria Brevibacterium iodinum RS16 and Methylobacterium oryzae CBMB20 on maize and sorghum-sudan grass hybrid under different concentrations of NaCl. Non-inoculated maize and sorghum-sudangrass hybrid showed 33.4% and 20.0% reduction in seed germination under highest NaCl (150 mM) level tested. However, under the same NaCl concentration, co-inoculation with B. iodinum RS16 and M. oryzae CBMB20 PGP strains increased the seed germination in maize (16.7%) and sorghum-sudangrass hybrid (4.4%). In Gnotobiotic growth pouch experiments conducted for maize and sorghum-sudangrass hybrid, co-inoculation of PGP B. iodinum RS16 and M. oryzae CBMB20 mitigated the salinity stress and promoted root length by 22.9% and 29.7%, respectively. Thus the results of this study could help in development of potential bioinoculants that may be suitable for crop production under saline conditions.

• 한국약용작물학회지
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• 제25권4호
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• pp.238-243
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• 2017

Background: The production method of ginseng seedlings for ginseng cultivation is very important to ensure healthy rooting system as well as high quality, and yield of the resultant plants. This study was carried out to compare the growth characteristics of 2-year-old ginseng plants that were produced from seedlings grown in self soil nursery (SSN), nursery soil (NS) or hydroponic culture (HC). Methods and Results: The shading prop used was composed of four-layered 4 polyethylene (blue 3 + black 1) shade screen. The management of main field was done by inserting oil cake (1,200 kg/10 a) and then allowing Sudan grass to grow for a year. Seedling transplantation was carried out on April 6. Root growth was measured on October 25. Root weight was observed to be excellent at 6.0 g, following SSN transplantation. Root length was 21.2 cm for HC seedlings, but these plants had a physiological disorder (i.e., rusty root), in 83.5% plants of this treatment. The ratio of PD/PT (protopanaxadiol saponins / protopanaxatriol saponins) was higher in NS seedlings. Plant analysis revealed that Fe content was lower in HC seedlings with high rustiness. The growth of 2-years-old ginseng was different following these varying seedling cultivation methods, but seedlings from NS were not different from those grown in SSN. Conclusions: For the propagation of 2-year-old ginseng plants, NS seedlings may be a good substitute for SSN seedlings.

• 한국유기농업학회지
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• 제7권1호
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• pp.79-90
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• 1998

제주지역에서 前作物로 綠肥作物을 재배하여 破碎$\cdot$耕耘하고 폴리에칠렌 투명비닐로 1997년 8월 5일부터 8월 29일까지 太陽熱土壤消毒후 가을감자를 재배한 결과를 요약하면 다음과 같다. 멀칭기간중의 토양깊이 10cm에서 최고지원은 $42^{\circ}C$였다. 綠肥作物栽培 및 멀칭에 의해 토양의 pH 및 有機物含量은 높아졌다. 前作物로 재배한 綠肥作物의 生體收量은 sudangrass 交雜種, 콩, 알팔파 순으로 많앙Tejs 반면 멀칭 太陽熱土壤消毒후 가을감자의 地上部生體重, 塊莖收量 등의 생육형질은 콩 + 가을감자재배구가 가장 양호한 것으로 나타났다. 이상의 결과로 볼 때 여름철 고온기에 綠肥作物栽培후 비닐 피복 太陽熱消毒에 의해 작물의 생육형질이 향상될 뿐만 아니라 土壤消毒의 가능성도 있을 것으로 생각된다.

• 한국초지조사료학회지
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• 제35권2호
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• pp.99-104
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• 2015

본 시험은 2013년부터 2014년까지 경북 성주에서 여름 파종 수수류 교잡종의 생육특성, 품질 및 수량성을 평가하기 위하여 실시하였다. 시험설계로 품종을 처리로 한 6처리 3반복으로 배치하였다. 파종기는 2013년과 2014년 7월 31일에 조파하였고 수확은 2013년 11월 3일과 2014년 11월 5일에 하였다. 출수기는 2년 평균이 sordan79 10월 5일 sprint 10월 8일 SX17 10월 9일 순으로 조사되었고, 2013년 수수류 교잡종의 DM수량은 SX17, Sordan79 및 Sprint 가 각각 24.2, 23.9 및 23.4톤/ha으로 SS405, PACF8350 및 Sugar grazer 21.5, 20.6 및 20.5톤/ha 보다 높았으며 (p<0.05), DDM수량 역시 비슷한 경향을 보였다. 2014년 DM수량은 SX17, Sordan79 및 Sprint가 20.8, 19.3 및 20.0톤/ha로 다른 3품종 보다 높았으며(p<0.05), DDM수량 역시 비슷한 경향을 보였다. ADF와 NDF의 함량은 비교적 낮았으며 반면에 DDM 함량은 높아 사료가치가 우수하였다. 본 시험의 결과에 의하면 여름 파종 시 전통형(출수형) 수수-수단그라스 교잡종 SX17, Sordan79와 수단그라스-수단그라스 교잡종 Sprint가 늦은 개화형(비출수형) 수수-수단그라스 교잡종 PACF8350이나 수수-수수교잡종 SS405와 Sugar grazer 보다 수량측면에서 남부지방에서 유리할 것으로 생각된다.

• 한국작물학회지
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• 제34권4호
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• pp.396-399
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• 1989

본 시험에서는 일반숙전과 신개간지 토양에서 비료 증시용이 진주조의 생산성 및 품질에 미치는 영향을 구명하기 위하여 수원 ,청주, 전주, 진주 및 북제주에서 1986∼'88년 시험한 주요 결과는 다음과 같다. 1. 질소시용량이 증가함에 따라사 초장, 청예수량, 및 단백질함량이 증가하였다. N30kg/10a까지는 도복이 많이 되었다. 초고가 1m 이상되고 일기가 불순하여 도복이 우려되면 지표면에서부터 20cm 남기고 예취하는 것이 바람직하였다. 2. 일반숙전토양에서 N45∼60kg/10a 구에서 평균 청예수량 12.7∼13.4t/10a이 생산되어 가장 증수되었다. 인산, 가리비료의 증시효과도 있었다. 특히 가리사료의 증시효과가 컸다. 3. 신개간지 토양에서도 개양식 배비구(60-60-40-4000kg/10a)에서 청예수량 12.6t/10a이 생산되어 표준비구에 비하여 38％ 증수되었다, 진주조 청예수량이 수수류나 옥수수에 비하여 월등히 증수되었다. 4. 청예용 식물체의 조단백질함량도 진주조 1회 예취에서 11.8％, 4회예취 평균 16.1％로 월등히 높았다. 소화율도 57.6∼63.4％로 옥수수 46.3％, 수수/수단그라스 47.3∼57.6％에 비하여 높았다.

• 한국유기농업학회지
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• 제20권1호
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• pp.37-48
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• 2012

본 연구는 유기재배 배 과수원에서 녹비작물 재배에 의한 식물양분의 환원효과를 알아보기 위해서 수행되었다. 처리는 녹비작물 재배방법에 따라서, 1) 방임초생, 2) 겨울철 녹비작물 재배(호밀+헤어리베치), 3) 겨울철 녹비작물+여름철 녹비작물[그린솔고(수단그라스 품종($Sorghum$ $bicolor$ L.))+네마장황(클로타라리아 품종($Crotalaria$ $juncea$ L.))]으로 나누었다. 겨울철 녹비작물인 호밀과 헤어리베치는 각각 6.4kg/10a와 3.0kg/10a로 2008년 10월 17일에 파종하였으며, 여름철 녹비작물인 그린솔고와 네마장황은 각각 2.0kg/10a와 3.0kg/10a로 2009년 6월 4일에 전남 보성의 배 과수원 독농가에 처리하였다. 녹비작물의 건물중은 겨울+여름녹비작물>겨울녹비작물>방임초생 순으로 나타났다. 녹비작물의 건물중의 차이는 전질소와 인산 그리고 칼륨의 환원량에도 영향을 미쳤으며, 10-12년생 '신고' 배나무가 필요로 하는 질소와 인산 그리고 칼륨의 요구도에 비교해서 다소 많은 양을 공급한 것으로 판단되었다. 이러한 과다한 무기성분 공급량은 토양의 유기물과 칼륨 그리고 마그네슘 농도를 적정농도 이상으로 증가시켰다. 하지만 배나무의 영양상태의 지표인 잎의 무기성분은 녹비작물 연용에 따른 별다른 영향을 받지 않은 것으로 사료되었다.