• 미생물학회지
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• 제25권1호
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• pp.80-85
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• 1987

Zymomonas mobilis에 관찰된 ethanol 저해작용의 원인을 규명하고자 회분 및 연속배양에서 얻은 균의 생존성고 균체내의 지방산 분포를 조사하였다. 그 결고 ethanol 농도의 변화는 균체의 지방산 분포에 직접적인 영향을 주었고, 또한 균의 생존성과도 밀접한 연관이 있음을 알았다.

• 한국식품영양과학회지
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• 제24권4호
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• pp.582-586
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• 1995

The effects of cultural temperature and nutritional components on the production of ethanol using juice of Pyrus serotina as the substrate for Saccharomyces cerevisiae ATCC 4124 were studied. After anaerobic cultivation in 5L flask of a defined pear juice at 2$0^{\circ}C$, ethanol concentration of 11.5%(v/v) could be obtained. The addition of a small amount of K2S2O5 was essential for the successful production of ethanol. Ethanol concentration could be further enhanced by supplementing a small of various complex nitrogen sources. When 0.05% of yeast extract and 0.05% of (NH4)2HPO4 were added to a defined medium, ethanol concentration obtained after 7 day cultivation at 2$0^{\circ}C$ was 12.3%.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2013년도 제45회 하계 정기학술대회 초록집
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• pp.266.2-266.2
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• 2013

According to advanced nanotechnology, the nanostructured materials with various kinds and shape are synthesized easily or produced by process. Recently, researches about interaction between the nanostructured materials and biological system have been progressed actively. The surface topography may influence cellular responses, for example cell adhesion, cell morphology. In this work, we synthesized vertically aligned silicon nanowires (SiNWs) on the Au-covered Si(111) wafer by chemical vapor deposition (CVD) method. We accomplished to control of the SiNWs diameter by regulating thickness of Au film such as 1 nm and 10 nm. These substrates did not isolate cells and just provided surface topography for cell culture. Human Embryonic Kidney 293T cells (HEK 293T cells) were cultured on these substrates for 2 days. We studied the nanotopographical effects on cell morphology, adhesion, and growth which are evaluated on each SiNWs substrate comparing bare glass as control.

• 미생물학회지
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• 제22권2호
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• pp.97-101
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• 1984

To investigate the biodegradation pattern of rice straw, mainly composed of cellulose, hemicellulose and lignin components, by the isolate stran Bacillus subtilis $DO_4$, the change of cell population was observed on CMC (carboxymethyl cellulose), larch wood xylan and lignosulfonate as a carbon source respectively. Also, the transition pattern of enzyme activities of cellulase and xylanase and lignin contents was measured on rice straw and mixed substrate according to growth. The results in these experiments revealed that xylanase activity was first appeared and cellulase activity in the next, while lignin component was almost not changed through the culture period.

• 미생물학회지
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• 제9권3호
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• pp.95-102
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• 1971

The growth characteristics of Candida tropicalis KIST 351 on gas oil substrate under different culture conditions were investigated and the preliminary animal feeding experiments using this yeast as a partial substitute of fish meal was also conducted. The yeast assimilates effectively n-paraffins in gas oil ranging from $C_{16}$ to $C_{16}$ with its maximum cell growth at $33^{\circ}C$ and pH 5.5 with aeration of 3 vvn and agitation of 900 rpm. The optimal concentrations of nitrogen sources, $HK_2PO_4$ and $Na_2HPO$ were 4, 2 and 0.5g/1, respectively. Ferrous sulfate, manganese sulfate and zinc sulfate showed positive effect to cell growth with the optimal range of 5-10 ppm. In the feeding experiment with 3 and 5% incorporation of the gas oil grown yeast, neither adverse effects on growth of chicks nor toxic effect were observed. Protein content of the dried cell was 58.8% and its amino acid composition compared well with other single-cell protein products and FAO reference protein.

• International Journal of Industrial Entomology and Biomaterials
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• 제41권2호
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• pp.28-35
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• 2020

Entomopathogenic Beauveria bassiana and Metarhizium anisopliae are well-known biological control agents worldwide, and have high potential in industrialization. However, their thermo-susceptibility limits long-term storage under high temperature conditions and insecticidal activity after application in target pests. The virulences of M. anisopliae JEF-003 and JEF-004, and B. bassiana JEF-006 and JEF-007 against bean bug (Riptortus pedestris), and the thermotolerance of conidia produced on three kinds of grains, sorghum, millet and Italian millet as substrates for solid cultures were investigated. Of the three grains, Italian millet was the suitable grains in the production of thermotolerant conidia, and a significant relationship between conidial thermotolerance and the media was demonstrated. This work suggests that biological characteristics of entomopathogenic fungi are altered by culture media, and these results will be a chance to understand the factors in the media which make such changes.

• 한국균학회지
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• 제21권4호
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• pp.316-322
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• 1993

Examination by scanning electron microscopy and potato-dextrose agar medium showed that the dry seeds of R. raetam were externally free of fungi. When planted in sandy loam soil, the seeds become colonized with eleven soilborne fungal species. The fungi were isolated on cellulose agar, pectin agar and lignin agar media. Aspergillus flavus, A. niger, Penicillium capsulatum and Fusarium oxysporum had broad occurrence and recovered on the three media. The production of hydrolytic enzymes by the isolated fungi depends on the substrate and species. P. capsulatum, P. spinulosum and A. niger had wide enzymatic amplitude and they were able to produce cellulolytic, pectolytic and lignolytic activities on corresponding substrates as well as on seed coat containing media. The lignolytic activities of the isolated species except Chaetomium bostrychods and Trichoderma viride were enhanced on applying the seed coat materials as C-source rather than using lignin. Soaking R. raetam seeds in culture filtrates of the most fungi grown on seed coat supplemented media induced pronounced and distinct stimulating effect on seed germination. The most effective filtrates were those of P. capsulatum, P. spinulosum and Sporotrichum pulverulentum.

• 생물환경조절학회지
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• 제8권1호
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• pp.19-29
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• 1999

무등산수박과 달고나수박을 관비재배 및 양액재배에 대하여 비교 실험한 결과, 엽수는 달고나수박이 많은 반면 엽면적은 무등산수박이 높게 나타났고, 생체중 및 건물중에 있어서도 무등산수박이 달고나수박 보다 더 높게 나타났다. 엽수, 엽면적, 줄기당 생체중 및 건물중 등은 양액재배에서 더 양호한 생장을 나타내었다. 재배방식에 관계없이 당도는 달고나 수박이 무등산수박보다 훨씬 더 높은 반면 과실무게는 무등산수박이 더 높게 나타났다. 수박의 엽병내 질소함량은 정식후 15일에는 11.4∼13.4mg/gFW, 과실비대기에는 17.1∼20.6mg/gFW, 정도로 상승하였다. 인산은 생육초기 3.7∼5.7mg/gFW 정도에서 수확기에 이르면 0.6∼l.1mg/gFW 정도로 감소하였다. 칼리는 생육초기에 5.8∼6.6mg/gFW 정도였으나 수분기부터는 4.0∼4.8mg/gFW 범위로 흡수되었다. 칼슘의 흡수는 생육초기에는 양액재배 수박이 3.4∼4.1mg/gFW 범위로 관비재배 수박의 2.5∼3.5mg/gFW 범위보다 약간 많은 것으로 나타났지만 생육이 진전됨에 따라 관비재배 수박의 흡수량이 양액재배 수박의 흡수량보다 많아지는 경향이었다. 마그네슘의 흡수는 생육초기에 관비재배에서 훨씬 더 용이하게 흡수되는 경향이었으나 수분기 부터는 0.9∼l.3mg/gFW 범위로 일정하였다. 본 실험결과 무등산수박의 양액재배를 통한 품질향상 및 주년재배의 가능성이 인정되었다.

• 한국미생물·생명공학회지
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• 제37권1호
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• pp.62-68
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• 2009

소나무잔나비버섯(Fomitopsis pinicola) 균사로부터 laccase를 생산하기 위한 최적 배양조건을 조사하였다. 합성 배지 중 MCM이 가장 높은 laccase활성을 나타내었으며, MCM의 조성을 2% dextrose, 0.4% peptone, 0.05% $NaH_2PO_4{\cdot}H_2O$, 0.05% $CaCl_2$로 각각 대체하였을 때 효소활성이 가장 우수하였다. 따라서 F. pinicola로부터 laccase를 생산하기 위한 최적 배지조건은 2% dextrose, 0.4% peptone, 0.05% $NaH_2PO_4{\cdot}H_2O$, 0.05% $CaCl_2$이다. 이상의 배지를 사용하여 $25^{\circ}C$에서 8일 동안 배양하였을 때 효소의 활성이 최대에 도달함을 확인하였다. ABTS를 기질로 사용한 activity staining을 통해 소나무잔나비버섯 균사체의 laccase 활성의 분자량이 43-55 kDa임을 확인하였으며, 배양액 중의 최적 pH와 온도는 각각 pH 3.0과 $80^{\circ}C$이었다.

• Applied Biological Chemistry
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• 제15권3호
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• pp.199-206
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• 1972

Aspergillus awamori가 생산하는 konjak mannan 분해 효소군(酵素郡)에 관한 연구로서 효소생산조건, 효소의 정제 및 성질, 효소분해에 미치는 감마선 조사(照射)의 영향을 조사하여 다음과 같은 결과를 얻었다. 1) 보존균주(保存菌株) 80여개중 konjak mannan 분해효소를 다량 생산하는 것으로 A. awamori를 선정하였다. 2) A. awamori를 밀기울 고체배양하는 경우 최적 배양일수는 3일, 살수(撒水)의 pH는 4, 살수량(撒水量)은 100%가 가장 좋았다. 3) A. awamori를 액체 진탕 배양하는 경우 최적 배양일수는 6일, 기본배지(基本培地)에 대한 추가탄소원(追加炭素源)으로는 xylose 0.1%, 질소원(窒素源)으로는 peptone 0.04%가 가장 좋았다. 4) A. awamori의 고체배양물에서 konjak mannan분해효소를 유안염석(硫安鹽析) 및 DEAE-Sephadex column chromatography에 의하여 정제(精製)하고 fraction I과 fraction II를 분리하였다. 5) fraction I과 fraction II의 작용최적 pH는 모두 4이었고 pH 안정(安定)범위는 각각 $3.5{\sim}5$ 및 $3{\sim}6$이었으며 konjak mannan에 대한 가수분해도는 각각 9%, 50%, guar gum에 대한 것은 각각 14%, 11%이 었 다. 6) 조효소액(粗酵素液)에 의한 konjak mannan의 가수분해도는 0.5 Mrad이상의 감마선 조사(照射)에 의하여 약간 촉진되었으며 특히 수화(水和)상태에서의 조사(照射)에 의하여 그 효과가 현저하였다. 7) konfak mannan의 감마선 조사(照射)는 점도(粘度)강하와 환원력(還元力)증가를 초래하였으며 건조상태보다는 수화(水和) 상태에서 그 효과가 현저하였다.