• Title/Summary/Keyword: Submerged treatment

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Immune Stimulation and Anti-Metastasis of Crude Polysaccharide from Submerged Culture of Hericium erinaceum in the Medium Supplemented with Korean Ginseng Extracts (수삼추출물 첨가 배지에서 배양된 노루궁뎅이버섯 균사체 심부발효물 조다당획분의 면역 및 암전이 억제활성)

  • Kim, Hoon;Park, Chang-Kyu;Jeong, Jae-Hyun;Jeong, Heon-Sang;Lee, Hyeon-Yong;Yu, Kwang-Won
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.38 no.11
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    • pp.1535-1542
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    • 2009
  • To find the new use of Korean ginseng and mushroom, crude polysaccharides were prepared from submerged cultures of Hericium erinaceum in the medium supplemented with Korean ginseng extracts. When we fractionated crude polysaccharides (HE-GE-CP-1, 3, and 5) from hot-water extracts of submerged cultures of H. erinaceum with ginseng extracts (1%, 3%, and 5% addition of total medium), the yields of HE-GE-CP-1, 3, and 5 were identified at 5.7, 5.1, and 4.8%, respectively. Among crude polysaccharide fractions, HE-GE-CP-5 was significantly higher (1.89-fold of the saline control) than those of HE-GE-CP-1 (1.64-fold) or HE-GE-CP-3 (1.76-fold) on mitogenic activity of splenocytes. HE-GE-CP-5 also had the more potent bone marrow cell proliferation (1.83-fold) rather than HE-CP or HE-GE-CP-1 or HE-GE-CP-3 (1.59- or 1.44- or 1.69-fold, respectively), and anti-metastatic activity as anti-cancer effect showed the highest prophylactic value (72.4% inhibition of tumor control) in 5% supplementation of ginseng extract. However, the lysosomal phosphatase of macrophage was significantly stimulated after HE-GE-CP-3 treatment (2.03-fold). In addition, the immunostimulating and anti-metastatic crude polysaccharide, HE-GE-CP-5, contained mainly neutral sugars (63.2%) with considerable amounts of uronic acid (19.3%) and a small amount of proteins (8.8%). HE-GE-CP-5 can stimulate immune system to inhibit tumor metastasis, and its anti-tumor metastasis may be associated with macrophages, splenocytes and Peyer's patch cells activation.

Methane Fermentation of Facultative Pond in Pond System for Ecological Treatment and Recycling of Livestock Wastewater (축산폐수 처리 및 재활용을 위한 조건성연못의 메탄발효)

  • Yang, Hong-Mo
    • Korean Journal of Environmental Agriculture
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    • v.19 no.2
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    • pp.171-176
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    • 2000
  • A wastewater treatment pond system was developed for treatment and recycling of dairy cattle excreta of $5\;m^1$ per day. The wastes were diluted by the water used for clearing stalls. The system was composed of three ponds in series. A submerged gas collector for the recovery of methane was installed at the bottom of secondary pond with water depth of 2.4m. This paper deals mainly with performance of methane fermentation of secondary pond which is faclutative one. The average $BOD_5$, SS, TN, and TP concentrations of influent into secondary pond were 49.1, 53.4, 48.6, and 5.3 mg/l, and those of effluent from it were 27.9, 45.7, 30.8, 3.2 mg/l respectively. Methane fermentation of 2.4-meter-deep secondary pond bottom was well established at $16^{\circ}C$ and gas garnered from the collector at that temperature was 80% methane. Literature on methane fermentation of wastewater treatment ponds shows that methane bacteria grow well around $24^{\circ}C$, the rate of daily accumulation and decomposition of sludge is approximately equal at $19^{\circ}C$, and activities of methanogenic bacteria are ceased below $14^{\circ}C$. The good methane fermentation of the pond bottom around $16^{\circ}C$, about $3^{\circ}C$ lower than $19^{\circ}C$, results from temperature stability, anaerobic condition, and neutral pH of the bottom sludge layer. It is recommended that the depth of pond water could be 2.4m. Gas from the collector during active methane fermentation was almost 83% methane, less than 17% nitrogen. Carbon dioxide was less than 1% of the gas, which indicates that carbon dioxide produced in bottom sludges was dissolved in the overlaying water column. Thus a purified methane can be collected and used as energy source. Sludge accumulation on the pond bottom for a nine month period was 1.3cm and annual sludge depth can be estimated to be 1.7cm. Design of additional pond depth of 0.3m can lead to 15 - 20 year sludge removal.

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Biofilter pretreatment for the control of microfiltration membrane fouling

  • Park, Jae-Hyung;Satoshi Takizawa;Hiroyuki Katayama;Shinichiro Ohgaki
    • Proceedings of the Membrane Society of Korea Conference
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    • 2003.05a
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    • pp.31-38
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    • 2003
  • A pilot scale biofilter pretreatment-microfiltration system (BF-MF) was operated to investigate the effect of biofilter treatment in fouling reduction of microfiltration. Biofiltration was expected to reduce the membrane fouling by removal of turbidity and metal oxides. The hollow-fiber MF module with a nominal pore size of 0.1$\mu$m and a surface area of 8m$^2$ was submerged in a filtration tank and microfiltration was operated at a constant flux of 0.5 m/d. Biofiltration using polypropylene pellets was performed at a high filtration velocity of 320 m/d. Two experimental setups composed of MF and BF/MF, i.e., without and with biofilter pretreatment, were compared. Throughout the experimental period of 9 months, biofilter pretreatment was effective to reduce the membrane fouling, which was proved by the result of time variations of trans-membrane pressure and backwash conditions. The turbidity removal rate by biofiltration varied between 40% to 80% due to the periodic washing for biofilter contactor and raw water turbidity. In addition to turbidity, metals, especially Mn, Fe and Al were removed effectively with average removal rates of 89.2%, 67.8% and 64.9%, respectively. Further analysis of foulants on the used membranes revealed that turbidity and metal removal by biofiltration was the major effect of biofiltration pretreatment against microfiltration fouling.

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A Study on Membrane Fouling Contaminants and Control in Enhanced Sewage Treatment by Submerged Membrane Bioreactor (침지형 분리막을 이용한 오수고도처리 공정의 막오염 원인물질 및 제어에 관한 연구)

  • Park, Chul-Hwi;Yun, Jae-Gon
    • Journal of Korean Society of Water and Wastewater
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    • v.18 no.5
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    • pp.619-627
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    • 2004
  • Purposes of this study were to examine closely the extracellular polymeric substances (EPS) which was a membrane fouling contaminant, to control detected EPS by powdered activated carbon (PAC) dosage etc. and to evaluate the possibility of practical reuse facility. With high removal efficiency of general pollutants, when the PAC is added to MBR, improvement of removal efficiency of $COD_{cr}$, and color was expected and treated wastewater can be reused. It was judged that the correlation between EPS and membrane fouling was very high. Carbohydrate and DNA in the EPS were judged to be cause of membrane fouling. If EPS could be controled, not only membrane fouling would be decreased but also operation time would be extended. In experiment of powdered activated carbon (PAC), characteristics of the best PAC for membrane fouling control were the particle size of $7{\mu}m$, lodine Number of 1,050, surface area of peat of $1,150m^2/g$. In lab test, operation time of MBR by PAC dosage of 200mg/gVSS was longer than one of MBR by without PAC dosage. Because EPS, especially carbohydrate and DNA, was controled successfully by PAC, membrane fouling in MBR could be decreased.

Topological View of Viscous Flow behind Transom Stern (트랜섬 선미 후방의 점성 유동장 Topology 관찰)

  • Kim, Wu-Joan;Park, Il-Ryong
    • Journal of the Society of Naval Architects of Korea
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    • v.42 no.4 s.142
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    • pp.322-329
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    • 2005
  • Viscous flows behind transom stern are analyzed based on CFD simulation results. Stern wave pattern is often complicated due to the abrupt change of stern surface curvature and flow separation at transom. When a ship advances at high speed, whole transom stern is exposed out of water, resulting in the so-called 'dry transom'. However, in the moderate speed regime, stern wave development in conjunction of flow separation makes unstable wavy surface partially covering transom surface, i.e., the so-called 'wetted transom'. Transom wave formation is usually affecting the resistance characteristics of a ship, since the pressure contribution on transom surface as well as the wave-making resistance is changed. Flow modeling for 'wetted transom' is difficult, while the 'dry transom modeling' is often applied for the high-speed vessels. In the present study CFD results from the RANS equation solver using a finite volume method with level-set treatment are utilized to assess the topology of transom flow pattern for a destroyer model (DTMB5415) and a container ship (KCS). It is found that transom flow patterns are quite different for the two ships, in conformity to the shape of submerged transom. Furthermore, the existence of free surface seems to after the flow topology in case of KCS.

비스판 변이주에 의한 활성아포 생산성 증가

  • Jeon, Gyeong-Dong;Lee, Gwang-Ho;Kim, Won-Seok;Baek, Hyeon-Dong
    • 한국생물공학회:학술대회논문집
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    • 2000.04a
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    • pp.275-278
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    • 2000
  • Bacillus polyfermenticus SCD which is commonly called as 'Bispan strain' has been appropriately used for the treatment of long-term intestinal disorders, since the live strains in the form of active endospores can successfully reach the target intestine. The goal of this study were to get the novel bispan mutant strain and to get higher spore number of bispan mutant in submerged fermentation. Bispan mutant KD21 was obtained using NTG mutagenesis of bispan strain, and bispan mutant was cultivated in 5 L jar fermenter. In this study, productivity and sporulation rate of the bispan mutant KD2l showed approximately 77% increase when compared with bispan strain.

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Effective Screening Method for Viviparous Germination of Rice

  • Ju, Young-Cheoul;Han, Sang-Wook;Park, Joong-Soo;Park, Kyeong-Yeol
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.45 no.2
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    • pp.103-107
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    • 2000
  • The viviparity of 28 rice varieties was tested at 25 days after heading(DAH), 35DAH, and 45DAH in the laboratory and field condition for 12 days. The incubation temperature was 20/l$0^{\circ}C$ (day/night), 25/15$^{\circ}$C$ and 30/20$^{\circ}$C$ in the laboratory test, and under field water conditions in the field test. The biggest varietal difference of viviparity was found in the laboratory test when examined at 45DAH with the 6-day incubation under 25/15$^{\circ}$C$ . At this conditions the mean viviparous ratio was 32.1 % with the range of 53.9 and the variance of 259.5. In the field test, the significant varietal difference in the viviparity was also found in the lodging treatment at 45 DAH for 6 days. Correlation coefficient analysis between the field and laboratory tests was highly significant from 4 days after incubation at 45 DAH and after 6-day incubation at 35 DAH, and correlation coefficient was higher as incubation days in the laboratory and submerged days under field water became longer. Considering the correlation between the field and laboratory tests, varietal difference of viviparity and convenience of testing, the laboratory test at 45 DAH for 6-day incubation under 25/15$^{\circ}$C$ was the most efficient evaluation method for the viviparity of rice cultivar.

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Butt 용접부에서 잔류응력이 피로균열성장거동에 미치는 영향에 대한 실험적 연구 1

  • 최용식;김영진;우흥식
    • Journal of Welding and Joining
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    • v.6 no.4
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    • pp.27-34
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    • 1988
  • The objective of this paper is to investigate the effect of residual stress on fatigue crack growth behavior. For this purpose, submerged arc welding was performed on SM50A steel plate and post weld heta treatment (PWHT) was followed. Residual stress distribution on the weld plate was determined by a hole drilling method and a series of .DELTA.P-const. and .DELTA.K-decreasing fatigue test were performed on the three different regions, i.e. weld metal, HAZ and base metla. Following conclusins were achieved. 1. In "as welded" specimens, tensile residual stresses were produced in the center portion of the specimen while compressive residual stresses were produced near the edges. In PWHT specimens, however, most of the residual stresses were disappeared. 2. The fatigue crack growth behavior in low .DELTA.K region was considerably affected by the presence of residual stress in both "as welded" and PWHT specimens. 3. Because of the relaxation of residual stresses in PWHT condition, the values of m increased from 2.62-2.78 (in the "as welded" condition) to 3.57-3.91 (in the "PWHT" condition)3.91 (in the "PWHT" condition)condition)

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Treatment of Industrial Wastewater with High Concentration of Hydrocarbons Using Membrane Reactors

  • Bienati, B.;Bottino, A.;Comite, A.;Ferrari, F.;Firpo, R.;Capannelli, G.
    • Membrane Journal
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    • v.17 no.2
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    • pp.112-117
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    • 2007
  • The application of membrane bioreactors for the depuration of wastewater coming from the washing of mineral oil storage tanks is described. Microfiltration hollow-fibre membranes were used in the submerged configuration. Filtration tests were carried out with a biomass concentration of about 15 g/L in order to assess the critical flux of the hollow fibre membrane used. Then particular care was taken in carrying out the performance runs in the sub-critical flux region. The reactor performance was very high, with removal efficiencies ranging between 93% and 97% also when the concentration of hydrocarbon was very high. Some kinetic parameters for the COD and the hydrocarbon removal were estimated.

Quality Changes of Salted Baechu with Packaging Methods during Long Term Storage (포장방법을 달리한 절임배추의 장기저장중 품질변화)

  • Han, Eung-Soo;Seok, Moon-Sik;Park, Ji-Hyun
    • Korean Journal of Food Science and Technology
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    • v.30 no.6
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    • pp.1307-1311
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    • 1998
  • Optimal packaging methods for the long term storage of salted winter baechu were investigated. Salted baechu was packaged individually in 20 ㎏ unit weight in LDPE (low density polyethylene), HDPE (high density polyethylene), PVC-box and then stored at $0^{\circ}C$ for 8 weeks. During storage, quality index of salted baechu were measured in terms of salinity, pH, reducing sugar content, total cell counts and lactic acid bacterial counts. Salted baechu deteriorated rapidly in PVC-box, and slowly in HDPE but sustained for 8 weeks in LDPE. In all treatment, salted baechu was maintained better at submerged parts in exudate, but deteriorated at emerged parts.

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