한국생물공학회:학술대회논문집
- 2000.04a
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- Pages.275-278
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- 2000
비스판 변이주에 의한 활성아포 생산성 증가
- Published : 2000.04.08
Abstract
Bacillus polyfermenticus SCD which is commonly called as 'Bispan strain' has been appropriately used for the treatment of long-term intestinal disorders, since the live strains in the form of active endospores can successfully reach the target intestine. The goal of this study were to get the novel bispan mutant strain and to get higher spore number of bispan mutant in submerged fermentation. Bispan mutant KD21 was obtained using NTG mutagenesis of bispan strain, and bispan mutant was cultivated in 5 L jar fermenter. In this study, productivity and sporulation rate of the bispan mutant KD2l showed approximately 77% increase when compared with bispan strain.
본 연구에서 비스판균에 비해 아포형성능이 우수한 변이주를 선별하고, 발효조 배양에서 활성아포 생산성의 증가를 확인하였다. 최종 선별된 변이주 KD21균주를 5 L jar fermenter에서 배양한 결과, 36시간만에 총균수는
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