• Animal Bioscience
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• 제36권11호
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• pp.1647-1654
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• 2023

Objective: Aging roosters typically exhibit subfertility with decreasing semen quality, furthermore Thai native roosters reared in rural areas are raised for a longer duration than their usual lifespan. The present study therefore aimed to assess the effect of selenium supplementation as an antioxidative substance in diets to improve the semen cryopreservation of aged roosters. Methods: Semen samples were collected from young (n = 20) and aged (n = 20) Thai native roosters (Pradu Hang Dum) at 36 and 105 weeks of age when starting the experiment, respectively. They were fed diets either non-supplemented or supplemented with selenium (0.75 ppm). Fresh semen quality and lipid peroxidation of fresh semen was evaluated before cryopreservation using the traditional liquid nitrogen vapor method. Post-thaw sperm quality and fertility potential were determined. Results: Advancing age is unrelated to decreasing fresh semen quality (p>0.05). However, lipid peroxidation in rooster semen depended on age, and the malondialdehyde (MDA) concentration increased in aged roosters (p<0.05). Selenium supplementation in diets significantly decreased the MDA concentration and increased the sperm concentration (p<0.05). In contrast, cryopreserved semen was affected by advancing rooster age, and selenium influenced sperm quality (p<0.05). Younger roosters had higher post-thaw sperm quality and fertility potential than aged roosters (p<0.05). Likewise, diet selenium supplements improved post-thaw sperm quality and fertility compared with the non-supplement group. Conclusion: Rooster's age does not influence the rooster sperm quality of fresh semen, while sperm cryotolerance and fertility were greater in young roosters than in aged roosters. However, sperm of aged roosters could be improved by dietary selenium supplementation.

• Journal of Animal Science and Technology
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• 제66권3호
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• pp.567-576
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• 2024

Subclinical ketosis (SCK) is a prevalent metabolic disorder that occurs during the transition to lactation period. It is defined as a high blood concentration of ketone bodies (beta-hydroxybutyric acid f ≥ 1.2 mmol/L) within the first few weeks of lactation, and often presents without clinical signs. SCK is mainly caused by negative energy balance (NEB). The objective of this study is to identify single nucleotide polymorphisms (SNPs) associated with SCK using genome-wide association studies (GWAS), and to predict the biological functions of proximal genes using gene-set enrichment analysis (GSEA). Blood samples were collected from 112 Holstein cows between 5 and 18 days postpartum to determine the incidence of SCK. Genomic DNA extracted from both SCK and healthy cows was examined using the Illumina Bovine SNP50K BeadChip for genotyping. GWAS revealed 194 putative SNPs and 163 genes associated with those SNPs. Additionally, GSEA showed that the genes retrieved by Database for Annotation, Visualization, and Integrated Discovery (DAVID) belonged to calcium signaling, starch and sucrose, immune network, and metabolic pathways. Furthermore, the proximal genes were found to be related to germ cell and early embryo development. In summary, this study proposes several feasible SNPs and genes associated with SCK through GWAS and GSEA. These candidates can be utilized in selective breeding programs to reduce the genetic risk for SCK and subfertility in high-performance dairy cows.

• Clinical and Experimental Reproductive Medicine
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• 제27권4호
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• pp.353-357
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• 2000

Objective: Extrauterine formation of decidua of stromal cells has been well described, particularly in the cervix and ovary. The apparent hormonal mechanisn of this phenomenon suggestes a relationship to endometriosis. Whether formation of ectopic decidua represents a marked progestational response of endometriosis or an independent peritoneal-stromal reaction to pregnancy is unclear. This study was designed to determine the prevalence of deciduosis in the patients whose fertility were proven. Design: Prospective study of patients who had undergone cesarean delivery without history of endometriosis. Materials and Methods: The study was performed in 179 full tenn pregnant women. During the cesarean section, the pelvic organs were thoroughly investigated and the biopsies were collected at the lesions suspicious endometriosis. And then microscopic examination of removed tissues were done. Results: Of the 179 patients who underwent cesarean delivery, 48 women (26.8%) had the lesions suspicious endometriosis such as adhesion, pigmented spots. The ovary was the most frequently ocurred site (79.2%). Microscopically, decidual cells were observed in 34 cases (70.8%) of 48 biopsed patients. Conclusion: Endometriosis has been known to be associated with subfertility. Our observations found the prevalence of deciduosis was 19.0% (34/179) in tenn pregnant women whose fertilites were proven. We suggests that the deciduosis maya manifestation of endometriosis during pregnancy. However, further follow up study should be done to confirm this clinicopathologic process.

• 한국산학기술학회논문지
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• 제21권4호
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• pp.210-217
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• 2020

본 연구의 목적은 배우자의 가사노동시간, 결혼만족도, 자녀에 대한 가치관, 전통적 성역할, 저출산 관련 사회서비스 정책이 기혼여성의 출산의향에 영향을 미치는지를 파악하고자 함에 있다. 기혼여성 1,000명을 대상으로 설문을 조사하였으며, 이중에서 향후 출산의향이 있다고 응답한 482명을 대상으로 로지스틱 회귀분석을 수행하였으며, 연구결과는 다음과 같다. 인구사회학적 요인으로서 연령이 유의미하였는데, 연령이 낮을수록 출산의향이 높았다. 또한 가족에 관련한 변인을 보면, 자녀 수, 배우자의 가사노동시간, 결혼만족도가 유의미한 변인으로서 자녀 수가 적을수록, 배우자가 가사노동에 할애하는 시간이 많을수록, 결혼만족도가 높을수록 출산의향이 높았다. 그리고 자녀에 대한 가치관, 출산지원정책에 대한 인지도가 영향을 미치는 요인으로 나타났는데, 잔에 대해 긍정적인 가치를 부여하고 있을수록, 정부의 출산지원정책에 대한 인지정도가 낮을수록 출산의향이 높았다. 특히 정부의 출산지원정책에 대한 인지도는 출산휴가와 난임지원에 집중되어 있었고 다른 정책에 대해 잘 모르는 경우가 많았다. 따라서 출산의향을 증진시키기 위해서 성역할과 가사노동에 대한 배우자의 분담에 대한 인식의 개선과 인식변화의 이행을 위한 일-가정양립 지원체계의 강화, 그리고 난임지원에 대한 확대 및 저출산관련 서비스정책에 대한 홍보 강화가 필요하다.

• 대한한방부인과학회지
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• 제31권4호
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• pp.147-164
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• 2018

Objectives: This study was performed to analyze the pattern identification in infertile women who participated in infertility support program of Cheongju-city. Methods: The project proceeded from October, 2016 to June 2017. Participants filled in the questionnaire which is composed of 33 symptoms before korean medical treatments. Data were collected from 17 participants. We classified symptoms into six pattern identifications based on previous research and rated each answer to score. Results: Throughout the study, the average age of 26 patients was $34.88{\pm}3.19$ years old, the average height was $162.51{\pm}4.99cm$, and average weight was $58.41{\pm}7.88kg$. The average duration of acupuncture treatment was $121.03{\pm}52.73$ days and the average times of acupuncture treatment was $27.42{\pm}10.77$ times. The average number of herbal medicine treatment was $2.65{\pm}0.69$, the majority of it was Chokyungjongok-tang. After the treatment, 3 patients of infertility became pregnant (11.54%) in twenty six patients. By analyzing pattern identification survey papers, We can classify infertile women into 6 pattern identification based on previous research. The majority of participants was identified as Blood deficiency and the other participants were identified as Kidney deficiency, Liver depression, Dampness-phlegm, Qi deficiency and Static blood in order of priority. Most of patients' pattern identification were combined with two kind of pattern identification. In terms of priority, Liver depression combined with Kidney deficiency, Blood deficiency combined with Kidney deficiency, Liver depression combined with Qi deficiency, Liver depression combined with Dampness-phlegm. Conclusions: After the study we confirmed that Korean traditional medicine is a safe treatment for infertile women. Further clinical study about herbal medicines of patients who have combined pattern identification is needed.

• Journal of Ginseng Research
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• 제41권4호
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• pp.578-588
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• 2017

Background: Elevated testicular temperature disrupts spermatogenesis and causes infertility. In the present study, the protective effect of enzymatically biotransformed Panax ginseng Meyer by pectinase (GINST) against chronic intermittent heat stress-induced testicular damage in rats was investigated. Methods: Male Sprague-Dawley rats (4 wk old, 60-70 g) were divided into four groups: normal control (NC), heat-stress control (HC), heat-stress plus GINST-100 mg/kg (HG100), and heat-stress plus GINST-200 mg/kg (HG200) treatment groups. Each dose of GINST (100 mg/kg and 200 mg/kg) was mixed separately with a regular pellet diet and was administered orally for 24 wk. For inducing heat stress, rats in the NC group were maintained at $25^{\circ}C$, whereas rats in the HC, HG100, and HG200 groups were exposed to $32{\pm}1^{\circ}C$ for 2 h daily for 6 mo. At week 25, the testes and serum from each animal were analyzed for various parameters. Results: Significant (p < 0.01) changes in the sperm kinematic values and blood chemistry panels were observed in the HC group. Furthermore, spermatogenesis-related molecules, sex hormone receptors, and selected antioxidant enzyme expression levels were also altered in the HC group compared to those in the NC group. GINST (HS100 and HS200) administration significantly (p < 0.05) restored these changes when compared with the HC group. For most of the parameters tested, the HG200 group exhibited potent effects compared with those exhibited by the HG100 group. Conclusion: GINST may be categorized as an important medicinal herb and a potential therapeutic for the treatment of male subfertility or infertility caused by hyperthermia.

• 대한한방부인과학회지
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• 제36권4호
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• pp.78-95
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• 2023

Objectives: This study aims to analyze the results of the 2022 Gyeonggi-do Oriental Medicine Fertility Treatment Support Project, review the outcomes and satisfaction of Oriental Medicine fertility support initiatives, and propose progressive fertility treatment support strategies by comparing them with the results of the past three years. Methods: Total of 242 women and 205 spouses participated in the Korean Medicine fertility treatment support project, which encompassed herbal medicine, acupuncture, and counseling treatments over a 3-month period, followed by a 3-month post-treatment follow-up. Data pertaining to patients' general, demographic, and fertility-related characteristics were collected before treatment initiation. During treatment, information regarding the treatments administered by Korean medical doctors was recorded, along with post-treatment outcomes and satisfaction levels. Safety assessments included pre- and post-treatment blood tests and monitoring for adverse events. Results: Among the 242 female subjects, 209 successfully completed the treatment program. Of these, 35 (16.7%) achieved pregnancy, with 30 (15%) attaining pregnancy through herbal monotherapy. Out of the 35 pregnancies, 17 were maintained, while 10 resulted in miscarriage. Notably, 83.8% of patients expressed satisfaction with the treatment outcomes. An analysis spanning three years revealed a continuous increase in the average age of patients, surpassing 38 years in 2022, a critical point in fertility decline age. Additionally, there was a notable rise in the prevalence of patients with a history of gynecological issues, advanced spouse age, and semen abnormalities, which is assumed to have contributed to the decrease in the pregnancy success rate. Conclusions: This study compares the clinical results of the 2022 Gyeonggi-do Korean Medicine Fertility Treatment Support Project with those of the past three years. Based on these findings, recommendations are made to enhance the project, including stricter age criteria for patient selection, enhanced specialized treatment tools for medical doctors, and combining Korean Medicine treatment and medical assisted reproductive technologies, all aimed at increasing pregnancy success rates. These results provide a foundation for the development of fertility support projects and related policies.

• 한국환경생태학회지
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• 제34권1호
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• pp.72-84
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• 2020

본 연구의 목적은 난임 여성을 위한 산림치유 프로그램 개발의 기초자료를 제공하는 데 있다. 본 연구는 탐색적 연구로 난임 여성의 정서적 특성과 정서적 특성에 따른 산림치유 요구도가 무엇인지 밝혀내어 산림치유 프로그램 개발과 운영을 위한 기초 자료를 제공할 것이다. 본 연구는 서울 소재 난임 전문병원에 내왕하는 난임 여성 200명을 대상으로 난임 여성의 정서적 특성과 산림치유 프로그램 요구도에 대한 33항목을 설문한 조사연구이다. 자료 분석은 SPSS 21.0 프로그램을 이용하여 난임여성의 정서적 특성과 산림치유 프로그램에 대한 요구 사이의 상관관계와 중요도를 파악하고자 빈도분석과 교차분석, 일원분산분석을 진행하였다. 난임 여성들의 정서적 특성은 임신에 대한 압박감, 불안 및 두려움, 우울, 절망감, 무기력, 외로움, 슬픔, 수치심 및 죄책감, 조급함과 답답함, 분노 및 과민함 등으로 나타났다. 난임 여성의 임신에 대한 압박감, 우울, 절망감, 무기력, 외로움, 슬픔과 분노와 과민함, 불안 및 두려움이 높게 나타났다. 난임 여성의 정서적 특성에 따른 산림치유 프로그램 요구도 차이를 알 수 있었다. 임신에 대한 압박감과 수치심 및 죄책감에 따라서 난임 부부 산림치유 프로그램의 운영 형태가 유의한 차이가 있는 것으로 나타났다. 불안과 두려움에 따라서 프로그램 참여 경험이 유의한 차이가 나타났고, 우울, 절망감, 무기력, 외로움, 슬픔에 따라서는 산림치유 프로그램에 참여하지 못하는 이유에 유의한 차이가 있는 것으로 나타났다. 조급함과 답답함에 따라서는 희망하는 산림치유 프로그램 동반 참여자에 대해 유의한 차이가 있는 것으로 나타났다. 분노와 과민함에 따라서 산림치유 프로그램 참여경험 및 난임 여성을 위한 산림치유 프로그램을 통한 자조모임의 효과에 대해 유의한 차이가 있는 것으로 나타났다. 본 연구의 분석 결과가 난임 여성의 심신건강 증진을 위한 산림치유 프로그램을 개발하는 데 기초자료로 활용되기를 기대한다.

• 의료법학
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• 제22권3호
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• pp.57-95
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• 2021

생명윤리법에 따라 우리나라의 배아 보존기간은 최대 5년이다. 그러나 최근 관련 과학기술 발달 및 보존기간 제한이 배아생성권자 권리를 제한한다는 요구가 제기됨에 따라 본 연구는 국내·외 법률을 검토하고 쟁점을 도출하였다. 첫 번째 쟁점으로 보존된 배아는 임신목적 착상 시도를 그 목적으로 하며, 과학적 근거 등 충분한 정보에 근거한 숙고를 통해 배아생성권자의 자율성이 보호되도록 하는 것이 중요하다는 것이다. 두 번째는 배아 보존기관 관리 의무 관련 규정이 필요하다는 것이다. 세 번째는 배아의 특수한 지위를 고려하여, 배아 생성, 보존, 폐기 행위가 최소한의 범위에서 이루어지는 사회적 분위기를 형성하는 것이다. 이 쟁점을 바탕으로 합리적 제도 개선 방안 제안의 첫 번째는 구체적 보존기간을 법에 명시하는 것보다, 충분한 설명과 적절한 동의가 행사될 수 있는 환경조성 및 보존기간의 연장 사유 확대 우선 도입이다. 두 번째는 보존기관 관리의무 뿐 아니라 그 결과 나타날 수 있는 우려 등 현장 전반을 고려한 제도화가 필요하다는 것이며, 마지막은 연구목적 제공을 위한 이관, 타인의 임신목적 기증 등 배아의 향후 활용 방안을 고려한 관리 방식의 도입을 제안한다. 이 과정은 태어난 아이와 가족관계 등 전반적인 고찰 뿐 아니라, 충분한 사회적 논의와 합의를 통한 방식이 되어야 할 것이다.