• 한국발생생물학회지:발생과생식
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• 제24권4호
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• pp.297-306
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• 2020

Repetitive changes in the intracellular calcium concentration ([Ca²⁺]i) triggers egg activation, including cortical granule exocytosis, resumption of second meiosis, block to polyspermy, and initiating embryonic development. [Ca²⁺]i oscillations that continue for several hours, are required for the early events of egg activation and possibly connected to further development to the blastocyst stage. The sources of Ca²⁺ ion elevation during [Ca²⁺]i oscillations are Ca²⁺ release from endoplasmic reticulum through inositol 1,4,5 tri-phosphate receptor and Ca²⁺ ion influx through Ca²⁺ channel on the plasma membrane. Ca²⁺ channels have been characterized into voltage-dependent Ca²⁺ channels (VDCCs), ligand-gated Ca²⁺ channel, and leak-channel. VDCCs expressed on muscle cell or neuron is specified into L, T, N, P, Q, and R type VDCs by their activation threshold or their sensitivity to peptide toxins isolated from cone snails and spiders. The present study was aimed to investigate the localization pattern of N and P/Q type voltage-dependent calcium channels in mouse eggs and the role in fertilization. [Ca²⁺]i oscillation was observed in a Ca²⁺ contained medium with sperm factor or adenophostin A injection but disappeared in Ca²⁺ free medium. Ca²⁺ influx was decreased by Lat A. N-VDCC specific inhibitor, ω-Conotoxin CVIIA induced abnormal [Ca²⁺]i oscillation profiles in SrCl₂ treatment. N or P/Q type VDC were distributed on the plasma membrane in cortical cluster form, not in the cytoplasm. Ca²⁺ influx is essential for [Ca²⁺]i oscillation during mammalian fertilization. This Ca²⁺ influx might be controlled through the N or P/Q type VDCCs. Abnormal VDCCs expression of eggs could be tested in fertilization failure or low fertilization eggs in subfertility women.

• The Korean Journal of Physiology and Pharmacology
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• 제26권2호
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• pp.125-133
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• 2022

Carbon monoxide (CO) is a known gaseous bioactive substance found across a wide array of body systems. The administration of low concentrations of CO has been found to exert an anti-inflammatory, anti-apoptotic, anti-hypertensive, and vaso-dilatory effect. To date, however, it has remained unknown whether CO influences atrial natriuretic peptide (ANP) secretion. This study explores the effect of CO on ANP secretion and its associated signaling pathway using isolated beating rat atria. Atrial perfusate was collected for 10 min for use as a control, after which high atrial stretch was induced by increasing the height of the outflow catheter. Carbon monoxide releasing molecule-2 (CORM-2; 10, 50, 100 μM) and hemin (HO-1 inducer; 0.1, 1, 50 μM), but not CORM-3 (10, 50, 100 μM), decreased high stretch-induced ANP secretion. However, zinc porphyrin (HO-1 inhibitor) did not affect ANP secretion. The order of potency for the suppression of ANP secretion was found to be hemin > CORM-2 >> CORM-3. The suppression of ANP secretion by CORM-2 was attenuated by pretreatment with 5-hydroxydecanoic acid, paxilline, and 1H-[1,2,4] oxadiazolo [4,3-a] quinoxalin-1-one, but not by diltiazem, wortmannin, LY-294002, or NG-nitro-L-arginine methyl ester. Hypoxic conditions attenuated the suppressive effect of CORM-2 on ANP secretion. In sum, these results suggest that CORM-2 suppresses ANP secretion via mitochondrial K_ATP channels and large conductance Ca²⁺-activated K⁺ channels.

• 농업생명과학연구
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• 제44권1호
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• pp.69-80
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• 2010

본 연구는 착색단고추의 유통실태를 분석하고 운영효율성을 측정하여 유통경로별 유통효율성을 비교분석한 것이다. 수출시장 (일본)의 경우에는 개별농가가 직접수출대행업체와의 계약을 통해 수출하는 것 (유형 III)이 영농조합법인 (유형 I:수출농단결성)과 지역농협을 통한 공동출하 (유형 II)의 경우보다 운영효율성이 높은것으로 분석되었다. 내수시장의 경로에서는 도매시장을 통한 경로가 가장 효율적인 것으로 분석되었는데 이는 도매시장을 통해 유통되는 물량은 대형유통업체와 계통판매되는 물량보다 비표준화된 물량이 많으므로 품질면에서 차이가 나지만 내수시장균형가격이 비교적 높게 형성됨으로써 품질에 상응하는 가격이 형성되지 않고 있는 실정이다. 그리고 유형IV의 경우는 유형 V, VI유형보다 유통비용 투입에 대한 산출수준이 비교적 유리한 것으로 나타났다.

• The Korean Journal of Physiology and Pharmacology
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• 제24권6호
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• pp.503-516
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• 2020

KCNQ family constitutes slowly-activating potassium channels among voltage-gated potassium channel superfamily. Recent studies suggested that KCNQ4 and 5 channels are abundantly expressed in smooth muscle cells, especially in lower urinary tract including corpus cavernosum and that both channels can exert membrane stabilizing effect in the tissues. In this article, we examined the electrophysiological characteristics of overexpressed KCNQ4, 5 channels in HEK293 cells with recently developed KCNQ-specific agonist. With submicromolar EC₅₀, the drug not only increased the open probability of KCNQ4 channel but also increased slope conductance of the channel. The overall effect of the drug in whole-cell configuration was to increase maximal whole-cell conductance, to prolongate the activation process, and left-shift of the activation curve. The agonistic action of the drug, however, was highly attenuated by the co-expression of one of the β ancillary subunits of KCNQ family, KCNE4. Strong in vitro interactions between KCNQ4, 5 and KCNE4 were found through Foster Resonance Energy Transfer and co-immunoprecipitation. Although the expression levels of both KCNQ4 and KCNE4 are high in mesenteric arterial smooth muscle cells, we found that 1 μM of the agonist was sufficient to almost completely relax phenylephrine-induced contraction of the muscle strip. Significant expression of KCNQ4 and KCNE4 in corpus cavernosum together with high tonic contractility of the tissue grants highly promising relaxational effect of the KCNQ-specific agonist in the tissue.

• ETRI Journal
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• 제42권4호
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• pp.575-584
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• 2020

Electromagnetic (EM) waves used to send signals under seawater are normally restricted to low frequencies (f) because of sudden exponential increases of attenuation (𝛼) at higher f. The mathematics of EM wave propagation in seawater demonstrate dependence on relative permeability (𝜇_r), relative permittivity (𝜀_r), conductivity (𝜎), and f of transmission. Estimation of 𝜀_r and 𝜎 based on the W. Ellison interpolation model was performed for averaged real-time data of temperature (T) and salinity (S) from 1955 to 2012 for all oceans with 41 088 latitude/longitude points and 101 depth points up to 5500 m. Estimation of parameters such as real and imaginary parts of 𝜀_r, 𝜀_r', 𝜀_r", 𝜎, loss tangent (tan 𝛿), propagation velocity (V_p), phase constant (𝛽), and α contributes to absorption loss (L_a) for seawater channels carried out by using normal distribution fit in the 3 GHz-40 GHz f range. We also estimated total path loss (L_PL) in seawater for given transmission power P_t and antenna (dipole) gain. MATLAB is the simulation tool used for analysis.

• Journal of Applied Biological Chemistry
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• 제63권3호
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• pp.235-241
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• 2020

An essential component of the hemostatic process during vascular damage is platelet activation. However, many cardiovascular diseases, such as atherosclerosis, thrombosis, and myocardial infarction, can develop due to excessive platelet activation. Isoscopoletin, found primarily in plant roots of the genus Artemisia or Scopolia, has been studied to demonstrate potential pharmacological effects on Alzheimer's disease and anticancer, but its mechanisms and role in relation to thrombus formation and platelet aggregation have not yet been discovered. This research investigated the effect of isoscopoletin on collagen-induced human platelet activation. As a result, isoscopoletin strongly increased cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP) levels in a concentration-dependent manner. In addition, isoscopoletin greatly phosphorylated inositol 1,4,5-triphosphate receptor (IP₃R) and vasodilator-stimulated phosphoprotein (VASP), known substrates of cAMP-dependent kinase and cGMP dependent kinase. Phosphorylation of IP₃R by isoscopoletin induced Ca²⁺ inhibition from the dense tubular system Ca²⁺ channels, and VASP phosphorylation was involved in fibrinogen binding inhibition by inactivating αIIb/β₃ in the platelet membrane. Isoscopoletin finally reduced thrombin-induced fibrin clot production and finally reduced thrombus formation. Therefore, this research suggests that isoscopoletin has strong antiplatelet effects and is likely to be helpful for thrombotic diseases involving platelets by acting as a prophylactic and therapeutic agent.

• Nuclear Engineering and Technology
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• 제54권3호
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• pp.1126-1135
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• 2022

The MARS code has been assessed for the prediction of onset of flow instability (OFI) in a vertical channel. For assessment, we built an experiment database that consists of experiments under various geometry and thermal-hydraulic condition. It covers pressure from 0.12 to 1.73 MPa; heat flux from 0.67 to 3.48 MW/m²; inlet sub-cooling from 39 to 166 ℃; hydraulic diameters between 2.37 and 6.45 mm of rectangular channels and pipes. It was shown that the MARS code can predict the OFI mass flux for pipes reasonably well. However, it could not predict the OFI in a rectangular channel well with a mean absolute percentage error of 8.77%. In the cases of rectangular channels, the error tends to depend on the hydraulic diameter. Because the OFI is directly related to the subcooled boiling in a flow channel, we suggest a modified subcooled boiling model for better prediction of OFI in a rectangular channel; the net vapor generation (NVG) model and the modified wall evaporation model were modified so that the effect of hydraulic diameter and heat flux can be accurately considered. The assessment of the modified model shows the prediction of OFI mass flux for rectangular channels is greatly improved.

• 한국추진공학회지
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• 제8권1호
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• pp.81-84
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• 2004

KSR-III 축소형 연소기를 원형으로 하는 8채널 형태의 물냉각 칼로리미터를 설계/제작하였다. 설계는 1차원 경험식을 이용하였으며 시험전에 3차원 전산유동해석을 수행하여 연소시험을 위한 냉각 조건의 안전성을 확인하였다. 탄소침착물의 단열효과를 고려할 경우 노즐목 부근에서 예측한 열유속이 정확성을 가지는 것으로 확인되었으며 연소실 부근에서는 상대적으로 큰 오차를 보였다.

• JSTS:Journal of Semiconductor Technology and Science
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• 제16권1호
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• pp.112-117
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• 2016

This paper presents a half-rate current-integrating DFE receiver with sub-unit interval (sub-UI) inter-symbol interference (ISI) cancellation. By having a single additional DFE tap in each data path, the proposed DFE receiver can minimize BER degradation due to input pattern dependency and feedback tap latency problems in conventional current-integrating DFE receivers. The proposed DFE receiver was designed and fabricated in a 45 nm CMOS process, whose measurement results indicated that the BER bathtub width is increased from 0.235 UI to 0.315 UI (34% improvement) at $10^{-12}$ BER level.

• 대한전자공학회:학술대회논문집
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• 대한전자공학회 2002년도 하계종합학술대회 논문집(4)
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• pp.33-36
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• 2002

Multiple Description Coding(MDC) is a technique used to obtain two or more (often correlated) descriptions of a source, which are transmitted over different channels to receiver. Two descriptions of the source support two levels of reconstruction quality. When all the descriptions are received and used in the reconstruction, the source should be reconstructed with acceptable quality. In this work, we consider Multiple Description Scalar Quantizer(MDSQ) to wavelet transform domain. Conventional MDSQ schemes in wavelet domain considered description with equal weights at each sub-bands after quantization. But each sub-bands is unequal contribution to whole image quality. Therefore, we experiment the multiple description with unequal weight in each sub-bands.