• Archives of Pharmacal Research
• /
• v.27 no.3
• /
• pp.283-286
• /
• 2004

The methylene chloride soluble fraction of MeOH extract from the stem bark of Styrax japonica S. et Z. (Styracaceae) showed significant cytotoxicity by SRB method against five human tumor cell lines. Four known pentacyclic triterpenoids, oleanolic aldehyde acetate (1), erythrodiol-3-acetate (2), euphorginol (3), and anhydrosophoradiol -3-acetate (4) were isolated by activity-guided fractionation. Their structures were determined by chemical and spectral analysis. Compounds 1-4 were isolated from S. japonica for the first time.

• Proceedings of the PSK Conference
• /
• 2003.10b
• /
• pp.205.3-206
• /
• 2003

The genus Styrax (Styracaceae) is different from other genera of this family due to the production of resinous material, usually secreted when the barks and trunks are injured by sharp objects. This resin, in the past considered a miraculous remedy in several parts of Asia and America, has been used in traditional medicine to treat inflammatory diseases. The CH$_2$Cl$_2$ fraction of Styrax japonica showed significant cytotoxic activities by SRB method against five human tumor cell lines (A549, HCT-15, MES-SA, SK-OV-3, and SK-MEL-2). (omitted)

• Biomolecules & Therapeutics
• /
• v.18 no.4
• /
• pp.420-425
• /
• 2010

The antifungal effects and action mechanisms of styraxjaponoside A and B were investigated. Devoid of hemolytic effect, the compounds had significant effect against several human pathogenic fungal strains, with energy-independent manners. To understand the action mechanisms of the compounds, the flow cytometric analysis plotting the forward scatter and the side scatter, $DiBAC_4$(3) staining and DPH fluorescence analysis were conducted. The results indicated that the actions of the compounds were dependent upon the membrane-active mechanisms. The present study suggests that styraxjaponoside A and B exert their antimicrobial effects via membrane-disruptive mechanisms.

• Korean Journal of Environment and Ecology
• /
• v.21 no.3
• /
• pp.257-277
• /
• 2007

We carried out a phytosociological study on pine forests in Korea with the method of Zurich-Montpellier School. We collected the data of 252 $relev{\acute{e}}s$ from 45 sites in the pine forests throughout the Korean Peninsula and its attached islands. The vegetation of the pine forests was classified into one association, three communities and seven subcommunities as follows: A: Quercus mongolica-Pinus densiflora community, A-1: Typical subcommunity, A-2: Vaccinium korean urn subcommunity, A-3 : Rhododendron micranthum subcommunity, B: Quercus serrata-Pinus densiflora community, B-1: Typical subcommunity, B-2: Juniperus rigida subcommunity, B-3: Styrax japonica subcommunity, B-4: Eurya japonica subcommunity, C: Saso-Pinetum densiflorae Yim et al. 1990, and D: Castanopsis cuspidata var. sieboldii-Pinus densiflora community. The former three communities were integrated into the Lindero-Quercion mongolicae Kim 1990 em. 1992. The Castanopsis cuspidata var. sieboldii-Pinus densiflora community remained to be studied in future to determine the association. The communities of Quercus mongolica-Pinus densiflora community was distributed throughout the montane zone in central-northern part of the Korean Peninsula. Quercus serrata-Pinus densiflora community occupied widely in the sub-montane and hilly areas in central and Southern Korean Peninsula. The association of Saso-Pinetum densiflorae was found in Cheju Island. Castanopsis cuspidata var. sieboldii-Pinus densiflora community were distributed in the warm-temperate zone including islands off the south-west coast of the Peninsula.

• Journal of Life Science
• /
• v.18 no.7
• /
• pp.974-979
• /
• 2008

The powdery mildew, a fungal plant disease found in varieties of plant cultures, is occurred by attack with Fusarium sp., Sphaerotheca sp., Leveilluna sp., and Eryshipe sp.. In this study we investigated the control of Fusarium graminearum, a barley powdery mildew fungus, by natural plant extracts. Among the 900 plant extracts tested, Zanthoxylum schinifolium, Ligusticum acutilobum, Bidens frondosa L., Dictamnus dasycarpus, Evodia officinalis, Disporum sessile, Scopolia japonica Max., Styrax japonica S. et Z., Dictamnus dasycarpus Turcz., Sinomenium acutum Rehder et Wils., Eugenia aromaticum, Rubus parvifolius L., Reynoutria elliptica, Coptis chinensis, Paeonia lactiflora Pall., Rheum undalatum, Paeonia suffruticosa, Oenothera odorata Jacq., Euphorbia pekinensis Rupr., and Nepeta cataria were selected based on spore germination inhibition assay. Further mycelial growth inhibition assay with economical and safety considerations led us to finally select Z. schinifolium (sancho) for control of F. graminearum. To produce antifungal sancho extract, methanol was suitable for extraction and subsequent fractionations of the extract showed that the water residue mainly had antifungal activity. The sancho extract and its fractions showed minor antibacterial activity against different pathogenic or food spoilage bacteria, but they did not show any harmful effects against young tomato plant by treatment of $1,000\;{\mu}g/ml$ in green chamber test. These results suggested that the extract of sancho has high potentials on control of a powdery mildew fungus, F. graminearum.

• Korean Journal of Environment and Ecology
• /
• v.19 no.3
• /
• pp.312-326
• /
• 2005

The purpose of this study is to provide data for conservation and ecological management of Pinus densiflora forest by analyzing ecological characteristics and the change of Pin us densiflora community structure for fifteen years in Namsan(Mt.). The actual vegetation of Pinus densiflora forest was divided into total six types and fifteen detail types. According to the results of TWINSPAN analysis of Pinus densiflora forest, there were fourty-four plots(unit: 400 m') and it was classified into five community types. The dominant species were Pinus densiflora in canopy layer, Styrax japonica in the understory layer and Stephanandra incisa with Rhododendron mucronujatum in the shrub layer. The index of shannon's diversity was from 1.5980 to 1.1485 per $400m^2$and the range of species number was from seventeen to twenty-two. As a result of the change of Pin us densinora forest structure, the importance percentage(1.p.) of Pinus densi. nora$(I.P.:\;77.9\%\rightarrow50.6\%) was decreased, Styraxjaponica$(I.P.:\;5.3\%\rightarrow22.6\%), Prunus sargen $(I.P.:\;1.5\%\rightarrow9.2\%) and Stephanandra incisa$(I.P.:\;3.5\%\rightarrow7.7\%) were increased for fifteen years. Based on standard of $2,000m^2$unit area, shannon's diversity changed 1.1719 into 0.8829 and species number changed thirty-four into twenty-one.

• Korean Journal of Environment and Ecology
• /
• v.27 no.4
• /
• pp.462-472
• /
• 2013

This study was carried out to provide a basic data for preservation of Pinus desiflora forest as cultural landscape forest by analyzing characteristics of plant community of P. desiflora forest in Geumjeongsan(mountatin) in Busan city. In order to analyze plant community of P. densiflora in Geumjeongsan, we set up 10 study plots inside and 8 plots outside of Geumjeongsansung(mountain fortress, hereinafter 'Sansung')(unit area: $400m^2$), a total of 18 plots. TWINSPAN analysis divided these 18 study plots into 6 communities which are Querqus serrata-P. desiflora community, P. desiflora community, P. desiflora-Q. serrata community, P. thunbergii-P. densiflora community, P. densiflora-P. thubergii-Q. acutissima community, and P. densiflora-Platycarya strobilacea community. Importance Percentage (I.P.) of each area and DBH class distribution of main species showed that P. densiflora community would succeed to Q. serrata community or C. tschonoskii community. Analysis on tree age found out that communities in the Sansung were 32~37 years old and those outside the Sansung were 44~57 years old. Shannon's species diversity index ranged from 0.4826 to 1.2499. Regarding correlation between species, P. densiflora had negative correlation with Styrax japonica. Based on abovementioned result we expected ecological succession from P. densiflora community to Q. serrata community inside of the Sansung. Outside the Sansung, succession from P. densiflora-P. thunbergii community to C. tschonoskii-Q. serrata community was expected. In order to manage P. densiflora forest as cultural landscape forest, Q. spp in the understory and shrub layer and deciduous broad-leaved arboreal trees should be managed. Tree crown management of deciduous broad-leaved trees in competition with P. desiflora, is also required.

• The Korean Journal of Soil Zoology
• /
• v.5 no.2
• /
• pp.47-62
• /
• 2000

This study investigated the effect of soil animals at forest fire area, and it carried out the mountain located at Jundae Ri, Houeng-chen Myen, Ha-dong Gun, Kyoung-Nam Province, southern part of Korea, where burned out about 50 hectars on April 11, 1997. Vegetation of the examined area absolutely dominated with the pines of 7-14 cm in diameter and 20 to 30 years old and the rest were covered with mixed forest with a shrub such as the oak (Quereus mongolia Fisch, Quereus variabilis BI, Quereus dentana Thunb), snowbell(Styrax japonica, S, et, z), lacquer tree (Rhus trichocarpa Mig), azalea (Rhododendron mucronulatum Turcz), etc. And there were simple area organized as a herbaceous plant, and the burnt area was poor experimental sites, where litter layer and herbaceous plant disappeard due to fire, and the unburnt area was rich in surface plant, dead leaves, twigs, etc. But the ground cover vegetations were poor in the unburnt area. The distribution of each animal groups, the seasonal fluctuation in population density, the biomass of meso$.$macroarthropods and the relationship between soil animal and some environmental factors were investigated and analyzed at each experimental area. The result are summarized as follow: 1. Identificated 257,087 individuals of soil microarthropods were classified into 7 classes and 24 orders of Arachinida, Insecta, Chilopoda, Symphyla, Diplopoda, Isopoda and Oligochaeta., and identified 8,006 individuals of the total meso$.$macroarthropods were classified into 7 classes and 20 orders of Arachinida, Insecta, Chilopoda, Symphyla, Diplopoda, Isopoda and Oligochaeta. 2. Among the total soil microarthropods, Arachinida formed 70.9%, followed by Insecta for 28.4% and among the total meso$.$macroarthropod , Insecta formed 57.6%, followed by Chilopoda for 23.8%.

• Journal of Ecology and Environment
• /
• v.29 no.6
• /
• pp.593-602
• /
• 2006

Species composition, spatial distribution of major species, diameter and height classes distribution, and species diversity were .analyzed in the Korean red pine (Pinus densiflora, hereafter referred as pine) forest in the permanent quadrats, which were designed for Long Term Ecological Research (LTER). Collected data were compared with those from the other areas including urban center (Mt. Inwang and Hongneung) and boundary areas (Mts. Acha, Bukhan, Bulam, Cheonggye, Daemo, and Surak), and natural areas (Mts. Seolak, Songni, and Wolak) to clarify the ecological characteristics of pine forest on Mt. Nam. Species composition of pine forest on Mt. Nam showed a similarity with those of urban center but did a little and big differences with those on urban boundary and natural areas, respectively. Such differences that pine forest on Mt. Nam showed, were usually due to Styrax japonicus, Sorbus alnifolia, Oplismenus undulatifolius, Ailanthus altissima, Ageratina altissima and so on, which showed higher coverage there. Predicted from diameter and height classes distribution of tree species, pine forest on Mt. Nam showed a possibility to be replaced by a S. japonica. Considered that this replacer species is not only a sub-tree but also shade intolerant, such successional trend could be interpreted as a sort of retrogressive succession. Those on urban boundary and natural areas showed a difference by displaying probabilities to be maintained as themselves as an edaphic climax or succeeded to oak forests. Species diversity of pine forest on Mt. Nam was lower than those in urban boundary and natural areas due to excessive dominance of several species, which led to different species composition from the other areas. Plants, which produced the differences, were species that flourishes in the polluted industrial area (S. japonica and S. alnifolia), favors the disturbed site (O. undulatifolius), and exotic species (A. altissima and Eupatorium rugosum). Those results reflects that pine forest of Mt. Nam was exposed on severe environmental pollution and excessive human interferences.