• Plant Biotechnology Reports
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• v.5 no.4
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• pp.331-344
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• 2011

The well-conserved NBS domain of resistance (R) genes cloned from many plants allows the use of a PCR-based approach to isolate resistance gene analogs (RGAs). In this study, we isolated an RGA (CapRGC) from Capsicum annuum "CM334" using a PCR-based approach. This sequence encodes a protein with very high similarity to Rx genes, the Potato Virus X (PVX) R genes from potato. An evolutionary analysis of the CapRGC gene and its homologs retrieved by an extensive search of a Solanaceae database provided evidence that Rx-like genes (eight ESTs or genes that show very high similarity to Rx) appear to have diverged from R1 [an NBS-LRR R gene against late blight (Phytophthora infestans) from potato]-like genes. Structural comparison of the NBS domains of all the homologs in Solanaceae revealed that one novel motif, 14, is specific to the Rx-like genes, and also indicated that several other novel motifs are characteristic of the R1-like genes. Our results suggest that Rx-like genes are ancient but conserved. Furthermore, the novel conserved motifs can provide a basis for biochemical structural. function analysis and be used for degenerate primer design for the isolation of Rx-like sequences in other plant species. Comparative mapping study revealed that the position of CapRGC is syntenic to the locations of Rx and its homolog genes in the potato and tomato, but cosegregation analysis showed that CapRGC may not be the R gene against PVX in pepper. Our results confirm previous observations that the specificity of R genes is not conserved, while the structure and function of R genes are conserved. It appears that CapRGC may function as a resistance gene to another pathogen, such as the nematode to which the structure of CapRGC is most similar.

• Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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• v.39 no.6
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• pp.469-476
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• 2021

The framework plan for the development of an integrated underground space map was established of preventing ground subsidence. The mapping process is expected to be completed to the level of nationwide municipal government standards by end of this year. To facilitate the utilization of the integrated underground space map, paper-based drawings for specialized organizations in underground safety impact assessment have been provided since September 2018, and services for local government officials have been provided in the underground information utilization system since May 2019. However, the map is utilized based on the information at the time of the initial development of the map, without any updates, thereby resulting in a lack of accuracy and latest information. This has led to a decrease in the utilization and reliability of the information. Therefore, in this study, for the underground structures(subway, underground shopping mall, underground passage, underground roadway, underground parking lot, utility tunnel), which are the key components of the integrated underground space map, a standard format for the submission of completed drawings is designed in accordance with Article 42 (2) of the Special Act on Underground Safety Management, which aims at laying the foundation for establishing the updated system of the integrated underground space map. In addition, through the verification of the automatically updated underground structure data based on the standard format, the reliability of the data can be assured. This format is expected to contribute to the improved utilization of the integrated underground space map in the future.

• Animal Bioscience
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• v.36 no.4
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• pp.570-583
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• 2023

Objective: Fibroblast growth factors (FGFs) play critical roles in embryo development, and immune responses to infectious diseases. In this study, to investigate the roles of FGFs, we performed genome-wide identification, expression, and functional analyses of FGF family members in chickens. Methods: Chicken FGFs genes were identified and analyzed by using bioinformatics approach. Expression profiles and Hierarchical cluster analysis of the FGFs genes in different chicken tissues were obtained from the genome-wide RNA-seq. Results: A total of 20 FGF genes were identified in the chicken genome, which were classified into seven distinct groups (A-F) in the phylogenetic tree. Gene structure analysis revealed that members of the same clade had the same or similar exon-intron structure. Chromosome mapping suggested that FGF genes were widely dispersed across the chicken genome and were located on chromosomes 1, 4-6, 9-10, 13, 15, 28, and Z. In addition, the interactions among FGF proteins and between FGFs and mitogen-activated protein kinase (MAPK) proteins are limited, indicating that the remaining functions of FGF proteins should be further investigated in chickens. Kyoto encyclopedia of genes and genomes pathway analysis showed that FGF gene interacts with MAPK genes and are involved in stimulating signaling pathway and regulating immune responses. Furthermore, this study identified 15 differentially expressed genes (DEG) in 21 different growth stages during early chicken embryo development. RNA-sequencing data identified the DEG of FGFs on 1- and 3-days post infection in two indigenous Ri chicken lines infected with the highly pathogenic avian influenza virus H5N1 (HPAIV). Finally, all the genes examined through quantitative real-time polymerase chain reaction and RNA-Seq analyses showed similar responses to HPAIV infection in indigenous Ri chicken lines (R² = 0.92-0.95, p<0.01). Conclusion: This study provides significant insights into the potential functions of FGFs in chickens, including the regulation of MAPK signaling pathways and the immune response of chickens to HPAIV infections.

• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.202-202
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• 2022

Recently days, soybean production in paddy field is increasing, from 4,422 ha in 2016 to 10,658 ha in 2021 in Korea. It is easy for Phytophthora stem and root rot (PSR) occurring in paddy field condition, when it is poorly drained soils with a high clay content, and temporary flooding and ponding. Therefore PSR resistant soybean cultivar is required. The objective of this study is to identify QTL region and candidate genes relating to PSR resistance of the race in main soybean cultivation area in Korea. 210 soybean materials including cultivars and germplasm were used for inoculation and genome-wide association study (GWAS). Inoculation was conducted using stem-scar method with 2 replications in 2-year for the race 3053 from Kimje and 3617 from Andong. 210 materials were genotyped with Soya SNP 180K chip, and structure analysis and association mapping were conducted with QTLMAX V2. The results of inoculation showed that survival ratio ranged from 0% to 96.7% and mean 9.7% for 3053 and ranged from 0% to 100% and mean 7.6% for 3617. Structure analysis showed linkage disequillibrium (LD) was decayed below r²=0.5 at 335kb of SNP distance. Significant SNPs (LOD>7.0) were identified in Chr 1, 2, 3, 4, 5, 11, 14, 15 for 3053 and Chr 1, 2, 3, 7, 10, 14 for 3617. Especially, LD blocks (AX-90455181;15,056,628bp~AX-90475572;15,298,872bp) in Chr 2 for 3053 and 3067 were duplicated. 29 genes were identified on these genetic regions including Glyma.02gl47000 relating to ribosome recycling factor and defense response to fungus in Soybase.

• Journal of the Korean earth science society
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• v.29 no.1
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• pp.1-12
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• 2008

A geophysical mapping was performed for Hwasan caldera which is located in Euisung Sub-basin of the southeastern part of the Korean Peninsula. In order to overcome the limitation of the previous studies, remote sensing technic was used and dense potential data were obtained and analyzed. First, we analyzed geological lineament for target area using geological map, digital elevation model (DEM) data and satellite imagery. The results were greatly consistent with the previous studies, and showed that N-S and NW-SE direction are the most dominant one in target area. Second, based on the lineament analysis, highly dense gravity data were acquired in Euisung Sub-basin and an integrated interpretation considering air-born magnetic data was made to investigate the regional structure of the target area. The results of power spectrum analysis for the acquired potential data revealed that the subsurface of Euisung Sub-basin have two density discontinuities at about 1 km and 3-5 km depth. A 1 km depth discontinuity is thought as the depth of pyroclastic sedimentary rocks or igneous rocks which were intruded at the ring vent of Hwasan caldera, while a 3-5 km depth discontinuity seems to be associated with the depth of the basin basement. In addition, three-dimensional gravity inversion for the total area of Euisung Sub-basin was carried out, and the inversion results indicated two followings; 1) Cretaceous Palgongsan granite and Bulguksa intrusion rocks, which are located in southeastern part and northeastern part of Euisung Sub-basin, show two major low density anomalies, 2) pyroclastic rocks around Hwasan caldera also have lower density when compared with those of neighborhood regions and are extended to 1.5 km depth. However, a poor vertical resolution of potential survey makes it difficult to accurately delineate the detailed structure caldera which has a vertically developed characteristic in general. To overcome this limitation, integrated analysis was carried out using the magnetotelluric data on the corresponding area with potential data and we could obtain more reasonable geologic structure.

• Geophysics and Geophysical Exploration
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• v.6 no.3
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• pp.126-133
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• 2003

High-resolution shallow marine seismic surveys have been carried out for the resources exploration, engineering applications and Quaternary mapping. To improve the resolution of subsurface structure image, multichannel digital technique has been applied. The quality of the image depends on the vertical and horizontal resolution and signal to noise (S/N) ratio which are associated with the data acquisition parameters such as sample interval, common midpoint (CMP) interval and CMP fold. To understand the effect of the acquisition parameters, a test survey was carried out off Yeosu and the acquired data were analyzed. A 30 $in^3$ small air gun was used as a seismic source and 8 channel streamer cable with a 5 m group interval was used as a receiver. The data were digitally recorded with a shot interval of 2 s and sample interval of 0.1 ms. The acquired data were resampled with various sample intervals, CMP intervals and CMP folds. The resampled data were processed, plotted as seismic sections and compared each other. The analysis results show that thin bed structure with ${\~}1m$ thickness and ${\~}6^{\circ}$ slope can be imaged with good resolution and continuity and low noise using the acquisition parameters with a sample interval shorter than 0.2 ms, CMP interval shorter than 2.5 m and CMP fold more than 4. Because seismic resolution is associated with the acquisition parameters, the quality of the subsurface structure can be imaged successfully using suitable and optimum acquisition parameters.

• Journal of the Korean Crystal Growth and Crystal Technology
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• v.26 no.3
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• pp.115-120
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• 2016

$CeO_2$ is used as a co-catalyst with $TiO_2$ to improve the catalytic activity of $MnO_x$ and characterization of nano-sized powder is identified with de-NOx efficiency. A comparison between $MnO_x-CeO_2/TiO_2$ and single $CeO_2$ was conducted in terms of microstructural analysis to observe the behavior of $CeO_2$ in the ternary catalyst. The $MnO_x-CeO_2/TiO_2$ catalyst was synthesized by sol-gel method and the average particle size of the single $CeO_2$ is about $285{\mu}m$ due to the low thermal stability, whereas the particle size $MnO_x-CeO_2/TiO_2$ is about 130 nm. The strong interaction between Ce and Ti was identified through the EDS mapping by transmission electron microscopy (TEM). The improvement about 20 % of $de-NO_x$ efficiency is observed in the low-temperature ($150^{\circ}C{\sim}250^{\circ}C$) and vigorous oxygen exchange by well-dispersed $CeO_2$ is the reason of catalytic activity improvement.

• Korean Journal of Soil Science and Fertilizer
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• v.43 no.1
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• pp.113-118
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• 2010

Due to diverse soil-forming environments and different purposes of the soil classification, numerous soil classification systems have been developed worldwide. The World Reference Base for Soil Resources (WRB) and the Soil Taxonomy of the United States are well-known in Korea. However, the German Soil Systematics based on somewhat different principles from the two former systems is little-known. The objective of this paper is therefore to give a short overview of the principles of the German Soil Systematics. The German Soil Systematics consists of a six-level hierarchical structure which comprises soil divisions, soil classes, soil types, soil subtypes, soil varieties, and soil subvarieties. Soils in Germany are firstly classified into one of four soil divisions according to the soil moist regime: terrestrial soils, semi-terrestrial soils, semi-subhydric/subhydric soils, and peats. Terrestrial soils are subdivided into 13 soil classes based on the stage of soil formation and the horizon differentiation. Semi-terrestrial soils are differentiated into four classes regarding the source of soil moist: groundwater, freshwater, saltwater, and seaside. Semi-subhydric/subhydric soils are subdivided into two classes: semi-subhydric and subhydric soils. Peats are classified into two classes of natural and anthropogenic origins. Classes can be compared to orders of the U.S. Taxonomy. Classes are subdivided into 29 soil types with regard to soil forming-processes for terrestrial soils, into 17 types with regard to the soil formation for semi-terrestrial soils, into five types with regard to the content of organic matter for semi-subhydric/subhydric soils, and also into five types with regard to peat-forming processes for peats. The soil mapping units in Germany are types, which can be additionally subdivided into ca. 220 subtypes, several thousands of varieties and subvarieties using detailed nuances of morphologic features of soil profile. Soil types can be compared to great groups of the U.S. Taxonomy.

• Journal of the Korean Electrochemical Society
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• v.24 no.4
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• pp.120-132
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• 2021

Although the development of high-Nickel is being actively carried out to solve the capacity limitation and the high price of raw cobalt due to the limitation of high voltage use of the existing LiCoO₂, the deterioration of the battery characteristics due to the decrease in structural stability and increase of the Ni content. It is an important cause of delaying commercialization. Therefore, in order to increase the high stability of the Ni-rich ternary cathod material LiNi_0.6Co_0.2Mn_0.2O₂, precursor Ni_0.6Co_0.2Mn_0.2-x(OH)₂/xTiO₂ was prepared using a nanosized TiO₂ suspension type source for uniform Ti substitution in the precursor. It was mixed with Li₂CO₃, and after heating, the cathode active material LiNi_0.6Co_0.2Mn_0.2-xTi_xO₂ was synthesized, and the physical properties according to the Ti content were compared. Through FE-SEM and EDS mapping analysis, it was confirmed that a positive electrode active material having a uniform particle size was prepared through Ti-substituted spherical precursor and Particle Size Analyzer and internal density and strength were increased, XRD structure analysis and ICP-MS quantitative analysis confirmed that the capacity was effectively maintained even when the Ti-substituted positive electrode active material was manufactured and charging and discharging were continued at high temperature and high voltage.

• Economic and Environmental Geology
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• v.54 no.6
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• pp.717-731
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• 2021

We analyzed the characteristics of the habitat environment for the Seonam study area in Ulsan, the southern shore of the East Sea using bathymetry and seafloor environment data. The depth of the study area ranges from about 0 m to 23 m. In the west of the study area, the water depth is shallow with a gentle slope, and the water depth becomes deeper with a steep slope in the east. Due to the right-lateral strike-slip faults located in the continental margin of the East Sea, the fracture surfaces of the seabed rocks are mainly in the N-S direction, which is similar to the direction of the strike faults. Three seafloor types (conglomeratic-grained sandy, coasre-graiend sandy, fine-grained sandy) and rocky bottom area have been classified according to the analyses of the bathymerty, seafloor image, and surface sediment data. The rocky bottom areas are mainly distributed around Seaoam and in the northern and southern coastal area. But the intermediate zone between Seonam and coastal area has no rocky bottom. This intermediate area is expected to have active sedimentation as seawater way. The sandy sediments are widely distributed throughout the study area. Underwater images and UAV images show that Cnidarians, Brachiopods, Mollusks are mostly dominant in the shallow habitat and various Nacellidae, Mytilidae live on the intertidal zone around Seonam. Annelida and Arthropod are dominant in the sandy sediments. The distribution of marine organism in the study area might be greatly influenced by the seafloor type, the composition and particle size distribution of the seafloor sediments. The analysis of habitat environment mapping with bathymetry, seafloor data and underwater images is supposed to contribute to the study of the structure and function of marine ecosystem.