• Journal of the Korean Magnetic Resonance Society
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• v.16 no.2
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• pp.147-161
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• 2012

Human melanocortin-4 receptor (hMC4R) among MC-Rs, expressed in the brain, is in charge of the control on energy homeostasis and food intake. The structure and function of human MC4R have been studied to understand their essential function and roles. To investigate the structure and function, it is necessary to prepare sufficient amounts of proteins. However, their expression and purification is demanding and time-consuming due to their innate insoluble and toxic properties. The heterozygous mutations of hMC4R, exchange of Asp 90 to Asn located in second transmembrane, cause severe obesity in human. To obtain purified hMC4R wt-TM2 for structural studies, it was first over-expressed and purified by fast protein liquid chromatography (FPLC) and then solution NMR studies were performed to get high-resolution spectra. In here, we established optimized purification scheme to get more purified target peptide.

• Journal of Korean language education
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• v.23 no.1
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• pp.219-256
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• 2012

The aim of this study is to observe the trend of discourse study in language education and analyze the main issues by investigating the literatures related to discourse in Korean language education in the last ten years. This study observed the discourse study conducted in Korean language education from the perspectives of study subject, study method and study data. Moreover, based on the results, it estimated the achievements and effectiveness of the discourse study conducted in Korean language education. The subject of discourse study was mainly dealt with discourse function, discourse pattern, discourse marker, discourse structure. In the study methods, analysis of corpus and survey were mainly used as the study methods, and spoken corpus, written corpus and semi-spoken corpus were used as study materials. In particular, the semi-spoken corpus was used at a very high rate among them. This showed that discourse study in Korean language education was mainly focused on spoken corpus study. This study divided the detailed field of Korean language education into four fields of linguistic knowledge, communication function, teaching activities and learning activities, and observed the trends of discourse study in each field. Overall, it was recognized that relatively many studies were focused on linguistic knowledge, particularly in pragmatic perspective. It can be said that the study based on discourse has a language educational effectiveness in that it is based on actual data and improves practical communication skills in the environment of various languages.

• International Journal of Contents
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• v.15 no.1
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• pp.10-24
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• 2019

This study applied network agenda-setting theory to analyze the impact of the agenda-setting function of the media on certain issues by focusing on the agenda at the center of controversy, 'Creative Economy'. To this end, the study extracted the data referred to creative economy in the media and SNS from 1 January 2008 to 31 December 2014, and analyzed the data using the network analysis program UCINET and the Korean language analysis program Textom. The results of the present study show that, during the period under former President Lee (2008-2011), the media's creative economy agenda-setting function did not exert a significant impact on the agenda-setting within SNS. However, from 2012 when the government of former President Park Geun-hye had started, the agenda-setting function of the media starts to show increasingly strong influence on the agenda cognition in SNS. The central words and sub-words configuration forming the center of the semantic network moved in the direction of a high correlation, in addition to the gradually increasing correlation based on QAP correlation analysis. In 2014, the semantic networks of the media and SNS bore a close resemblance to each other, while the shape of networks and sub-words structure also had a high level of similarity.

• Structural Engineering and Mechanics
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• v.71 no.2
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• pp.197-210
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• 2019

Probabilistic buckling behavior of sandwich panel considering random system parameters using a radial basis function (RBF) approach is presented in this paper. The random system properties result in an uncertain response of the sandwich structure. The buckling load of laminated sandwich panel is obtained by employing higher-order-zigzag theory (HOZT) coupled with RBF and probabilistic finite element (FE) model. The in-plane displacement variation of core as well as facesheet is considered to be cubic while transverse displacement is considered to be quadratic within the core and constant in the facesheets. Individual and combined stochasticity in all elemental input parameters (like facesheets thickness, ply-orientation angle, core thickness and properties of material) are considered to know the effect of different degree of stochasticity, ply- orientation angle, boundary conditions, core thickness, number of laminates, and material properties on global response of the structure. In order to achieve the computational efficiency, RBF model is employed as a surrogate to the original finite element model. The stiffness matrix of global response is stored in a single array using skyline technique and simultaneous iteration technique is used to solve the stochastic buckling equations.

• Journal of IKEEE
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• v.23 no.1
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• pp.318-321
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• 2019

The apparent soil resistivity is used for estimating multilayer soil parameters, such as, layer's depth and soil resistivity. The apparent soil resistivity can be measured, and also can be calculated if soil parameters are given, becacuse the apparent soil resistivity is a function of these parameters. Therefore, any optimization algorithms can be used to find these parameters which make the calculated apparent soil resistivity close to the measured one. The equation for calculating the apparent soil resistivity is complicated and time consumed, because it is composed of an infinite integral which includes a zero order Bessel's function of the first kind. In this paper, a fast algorithm for calculating the apparent soil resistivity of horizontal multilayer earth structure has been presented using complex image method.

• East Asian mathematical journal
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• v.35 no.2
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• pp.239-257
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• 2019

The purpose of this study is to show that the topology is closely related to some subjects learned in school mathematics and then to give motivations for learning of the topology. To do this, it is showed that the topology is an abstracted device that deal with structure of limit and continuity introduced in school mathematics. This study took a literature study. The results of this study are as follows. First, the formal definition of general topology to structure open sets was examined. The nearness relation together with the closure operation was introduced and used to characterize for construction of general topology. Second, as definitions for continuity of function, we considered the intuitive definition, definition, structured definitions using open intervals and definition using open sets and then we investigated their roles. We also examined equivalent definition using the nearness relation which is helpful to understand continuity of function. Third, the sequence and its limit are treated in terms of continuous functions having the set of natural numbers and its extended set as domains. From these, it can be concluded that the convergence of sequence and the continuity of function are identified as functions that preserve the nearness relation and that the topology is a specialized tool for dealing with convergence and continuity.

• Smart Structures and Systems
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• v.31 no.1
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• pp.45-56
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• 2023

Vibration-based structural health monitoring is used to ensure the safety of structures by installing sensors in structures. The peak picking method, one of the applications of vibration-based structural health monitoring, is a method that analyze the dynamic characteristics of a structure using the peaks of the frequency response function. However, the results may vary depending on the person predicting the peak point; further, the method does not predict the exact peak point in the presence of noise. To overcome the limitations of the existing peak picking methods, this study proposes a new method to automate the modal analysis process by utilizing long short-term memory, a type of recurrent neural network. The method proposed in this study uses the time series data of the frequency response function directly as the input of the LSTM network. In addition, the proposed method improved the accuracy by using the phase as well as amplitude information of the frequency response function. Simulation experiments and lab-scale model experiments are performed to verify the performance of the LSTM network developed in this study. The result reported a modal assurance criterion of 0.8107, and it is expected that the dynamic characteristics of a civil structure can be predicted with high accuracy using data without experts.

• Biotechnology and Bioprocess Engineering:BBE
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• v.7 no.3
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• pp.150-154
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• 2002

Monoclonal antibodies (Mabs) have been used as diagnostic and analytical reagents since hybridoma technology was invented in 1975. In recent years, antibodies have become increasingly accepted as therapeutics for human diseases, particularly for cancer, viral infection and autoimmune disorders. An indication of the emerging significance of antibody-based therapeutics is that over a third of the proteins currently undergoing clinical trials in the United States are antibodies. Until the late 1980's, antibody technology relied primarily on animal immunization and the expression of engineered antibodies. However, the development of methods for the expression of antibody fragments in bacteria and powerful techniques for screening combinatorial libraries, together with the accumulating structure-function data base of antibodies, have opened unlimited opportunities for the engineering of antibodies with tailor-made properties for specific applications. Antibodies of low immunogenicity, suitable for human therapy and in vivo diagnosis, can now be developed with relative ease. Here, antibody structure-function and antibody engineering technologies are described.

• Proceedings of the Earthquake Engineering Society of Korea Conference
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• 2000.10a
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• pp.407-414
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• 2000

The friction pendulum type seismic isolation system (FPS) has been developed to provide a simple and effective way to achieve earthquake resistance for buildings . The major advantages are: the isolation frequency can be easily achieved by designing a curvature of the surface and does not depend on the supported weight of a structure. The function of carrying vertical load is separated to the function of providing horizontal stiffness. Next the friction provides sufficient energy dissipation to protect the structure from earthquake response and resistance to the weak external disturbances such as wind load and ground vibrations due to traffic. In this paper, the friction coefficients are evaluated from number of experiments on the FPS test specimens. The relations between friction coefficient and the test waveform, velocity, and pressure are reviewed and further works are discussed.

• BMB Reports
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• v.49 no.2
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• pp.73-80
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• 2016

Autophagy is a process tightly regulated by various autophagy-related proteins. It is generally classified into non-selective and selective autophagy. Whereas non-selective autophagy is triggered when the cell is under starvation, selective autophagy is involved in eliminating dysfunctional organelles, misfolded and/or ubiquitylated proteins, and intracellular pathogens. These components are recognized by autophagy receptors and delivered to phagophores. Several selective autophagy receptors have been identified and characterized. They usually have some common domains, such as motif, a specific cargo interacting (ubiquitin-dependent or ubiquitin-independent) domain. Recently, structural data of these autophagy receptors has been described, which provides an insight of their function in the selective autophagic process. In this review, we summarize the most up-to-date findings about the structure-function of autophagy receptors that regulates selective autophagy.