• Journal of Microbiology and Biotechnology
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• 제9권5호
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• pp.582-588
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• 1999

A Brevibacterium lactofermentum gene coding for a glucose-specific permease of the phosphoenolpyruvate-dependent phosphotransferase system (PTS) was cloned, by complementing an Escherichia coli mutation affecting a ptsG gene with the B. lactofermentum genomic library, and completely sequenced. The gene was identified as a ptsG, which enables an E. coli transformant to transport non-metabolizable glucose analogue 2-deoxyglucose (2DG). The ptsG gene of B. lactofermentum consists of an open reading frame of 2,025 nucleotides encoding a polypeptide of 674 amino acid residues and a TAA stop codon. The 3' flanking region contains two stem-loop structures which may be involved in transcriptional termination. The deduced amino acid sequence of the B. lactofermentum enzyme $II^{GIe}$ specific to glucose ($EII^{GIe}$) has a high homology with the Corynebacterium glutamicum enzyme $II^{Man}$ specific to glucose and mannose ($EII^{Man}$), and the Brevibacterium ammoniagenes enzyme $II^{GIc}$ specific to glucose ($EII^{GIc}$). The 171-amino-acid C-terminal sequence of the $EII^{Glc}$ is also similar to the Escherichia coli enzyme $IIA^{GIc}$ specific to glucose ($IIA^{GIc}$). It is interesting that the arrangement of the structural domains, IIBCA, of the B. lactofermentum $EII^{GIc}$ protein is identical to that of EIIs specific to sucrose or $\beta$-glucoside. Several in vivo complementation studies indicated that the B. lactofermentum $EII^{Glc}$ protein could replace both $EII^{ Glc}$ and $EIIA^{Glc}$ in an E. coli ptsG mutant or crr mutant, respectively.

• 정보저장시스템학회논문집
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• 제2권2호
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• pp.130-137
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• 2006

The HDD(hard disk drive) using Load/unload(L/UL) technology includes the benefits which are increased areal density, reduced power consumption and improved shock resistance than those of contact-start-stop(CSS). It has been widely used in portable hard disk drive and will become the key technology for developing the small form factor hard disk drive. The main objects of L/UL are no slider-disk contact or no media damage. For realizing those, we must consider many design parameters in L/UL system. In this paper, we focus on lift-off force. The 'lift-off' force, defined as the minimum air bearing force, is another very important indicator of unloading performance. A large amplitude of lift-off force increases the ramp force, the unloading time, the slider oscillation and contact-possibility. To minimize 'lift-off' force we optimizes the slider and suspension using the integrated optimization frame, which automatically integrates the analysis with the optimization and effectively implements the repetitive works between them. In particular, this study is carried out the optimal design considering the process of modes tracking through the entire optimization processes. As a result, we yield the equation which can easily find a lift-off force and structural optimization for suspension.

• 한국산학기술학회논문지
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• 제16권9호
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• pp.6315-6324
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• 2015

본 연구에서는 2008년 금융위기 이후 부동산 정책에서 많은 비중을 차지하는 서울의 아파트 전세가격에 가계경제가 어떤 영향을 미치는지 2009년 1월부터 2013년 12월까지 자료를 이용하여 VAR모형을 통해 실증분석하였다. 서울의 전세가격에 미치는 가계경제변수들은 서울 아파트 매매가격, 소비자물가지수, 고용률, 실질GNI, 가계대출금액으로 구성하였다. 분석결과에 따르면, 서울 아파트 전세가격은 단기적으로 가계경제변수들에 영향력을 받는 것으로 나타났다. 또한 가계경제변수들의 구조적 충격에 따른 서울 아파트 전세가격 변동의 상대적 기여도는 단기적으로는 서울 아파트 전세가격 자체 충격에 가장 큰 영향력을 받으며, 시간이 지날수록 가계변수들의 영향력이 커지는 것으로 나타났다. 본 연구결과를 통해 가계경제를 이루는 어떤 요인들이 주택전세가격에 많은 영향을 미치는지 파악할 수 있어 향후 주택가격 안정화를 위한 정책수립에 기여할 것으로 기대된다.

• 한국산학기술학회논문지
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• 제8권5호
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• pp.1186-1191
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• 2007

본 연구는 교차형 와이드 거더를 이용한 건축구조로 최소한의 슬래브 두께로 가능한 최장의 스팬을 확보할 수 있도록 함으로써, 층고를 높이지 않으면서 실내 공간에 자유롭고 다양한 변화를 가져올 수 있도록 하는 것이다. 즉, 본 연구는 교차형 와이드 거더를 응력분포에 따라 일정 간격으로 연속 배치함으로써, 낮은 층고에서 해당 경계 공간을 무주 공간으로 형성시킬 수 있도록 하는 것이다 본 연구의 수치적 타당성을 검토하기 위하여 3차원 횡력 설계가 가능한 프로그램인 Midas Gen을 이용하여 기존의 벽체 시스템과 교차형 Wide Girder에 의한 연성골조 시스템을 해석한 결과, 구조적으로 횡변위 및 층간변위와 바닥의 처짐 등이 기존의 벽체시스템과 비교하여 크게 불리하지 않으며, 전체적인 콘크리트 물량이 기존 벽체시스템과 비슷하며 건축적인 가변성을 확보하는데 큰 장점이 있는 것으로 나타났다.

• 한국산학기술학회논문지
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• 제14권9호
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• pp.4142-4148
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• 2013

경사지 과수의 효율적인 재배 및 관리를 위해 과수원 전용 고소 작업차량 사용이 늘어나고 있다. 이러한 이유로 작업자 안전을 위해 고소 작업차의 안정성에 대해 연구가 요구되고 있다. 본 연구는 경사지 흙길을 주행할 수 있는 4개의 바퀴와 2개의 직교 좌표계 적재함을 가진 과수원 차량의 안정성 평가에 대한 연구이다. 차량 메커니즘에 대한 다물체 동역학(MBD) 해석을 통하여 19.2, $34.6^{\circ}$의 좌우 및 상하 방향의 전복각을 계산할 수 있었다. 바퀴들의 주행 저항과 소요 동력을 결정하였다. 그리고 적재함 프레임의 유한요소해석(FEA)를 통하여 최대응력 146 MPa로 구조적으로 안정하다. 따라서 적재함을 가진 바퀴형 과수원 차량은 정적 및 동적 안정성을 가짐을 알 수 있었다.

• 한국산학기술학회논문지
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• 제12권9호
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• pp.4216-4222
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• 2011

본 연구는 조적 끼움벽을 고려하지 않은 기존 학교건물의 비탄성 거동과 조적 끼움벽을 고려한 기존 학교 건물의 거동특성을 비교 검토하였다. 해석 결과와 실험 결과를 비교했을 때 하중-변위 곡선과 소성힌지 분포상태에서 유사함을 확인할 수 있었다. 따라서 조적 끼움벽을 고려한 비선형 정적해석의 유효성을 검증하였다. 골조내부가 전부 조적벽으로 채워진 경우 조적벽체의 영향에 의하여 초기 강성과 강도가 증가하고 항복 전까지는 근사한 거동을 보여주며 항복이후에는 변형이 커질수록 오차가 증가하며, 골조높이의 2/3 높이가 조적벽으로 채워진 경우 초기 강성과 항복강도가 단순골조에 비하여 약간 크게 나타나고 있으며, 조적벽체의 균열이 발생한 이후에는 급격히 강도가 저하되고 있다. 골조높이의 1/3 높이가 조적벽으로 채워진 경우 초기 강성과 항복강도가 단순골조와 비슷하며 항복점은 오히려 단순 골조의 항복점보다 저하되나, 최대강도는 단순 골조와 유사하다. Pushover 해석에 의한 하중-변위 관계곡선과 실제 실험에 의한 하중-변위 관계곡선을 비교해 보면 항복 전까지는 근사한 거동을 보여주며 항복이후에는 변형이 커질수록 오차가 증가하나 실제 구조물에서는 변형의 한계가 존재하므로 해석모델로서 유용하게 사용할 수 있다.

• Structural Engineering and Mechanics
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• 제74권3호
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• pp.395-406
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• 2020

Many prefabricated concrete frame joints have been proposed, and most of them showed good seismic performance. However, there are still some limitations in the proposed fabricated joints. For example, for prefabricated prestressed concrete joints, prefabricated beams and prefabricated columns are assembled as a whole by the pre-stressed steel bar and steel strand in the beams, which brings some troubles to the construction, and the reinforcement in the core area of the joints is complex, and the mechanical mechanism is not clear. Based on the current research results, a new type of fabricated joint of prestressed concrete beams and confined concrete columns is proposed. To study the seismic performance of the joint, the quasi-static test is carried out. The test results show that the nodes exhibit good ductility and energy dissipation. According to the experimental fitting method and the "fixed point pointing" law, the resilience model of this kind of nodes is established, and compared with the experimental results, the two agree well, which can provides a certain reference for elasto-plastic seismic response analysis of this type of structure. Besides, based on the analysis of the factors affecting the shear capacity of the node core area, the formula of shear capacity of the core area of the node is proposed, and the theoretical values of the formula are consistent with the experimental value.

• Journal of Animal Science and Technology
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• 제63권5호
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• pp.1114-1125
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• 2021

Supplying a sufficient amount of high-quality colostrum is a prevailing challenge in Hanwoo calf production systems. This study aimed to describe the efficiency of a lacteal-derived colostrum replacer versus natural bovine colostrum (from Holstein) fed to Hanwoo calves. Forty newborn Hanwoo calves (25 males and 15 females; 30.7 ± 3.15 kg body weight [mean ± standard deviation]) were blocked by sex and birth weight and fed either natural colostrum or a commercial colostrum replacer. Calves displayed a narrow difference in the evolution of body weight and structural body dimensions when they received a commercial versus natural colostrum. Minor differences existed in the starter and total dry matter consumption between calves fed natural or commercial colostrum. Although the colostrum source had no significant effects on days to first diarrhea (average of 7.6 days; p = 0.17), the duration of diarrhea was longer in natural colostrum-fed calves (3.2 vs. 4.5 days; p = 0.04). Consumption of natural colostrum resulted in greater mean serum immunoglobulin G (IgG) on day 2 (14.7 vs. 10.8 ± 0.92 mg/mL) and day 7 (8.21 vs. 5.12 ± 0.82 mg/mL) of calf life, resulting in a greater proportion of commercial colostrum-fed calves (50% vs. 15%; day 2) to experience failure of passive transfer of immunity (serum IgG < cutoff point of 10 mg/mL). Overall, growth rate, body frame development, and incidence of diarrheal disease were not different in both groups. These results suggested that the colostrum replacer product tested in this study could be an alternative to natural colostrum derived from Holstein cow in securing calf growth and health in Hanwoo calf-rearing operations.

• 한국의류산업학회지
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• 제21권3호
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• pp.346-355
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• 2019

This study aimed to determine the factors of the make-up artist experience and establish their effects on brand loyalty through the mediation of trust and brand satisfaction. The step of first surveyed the make-up artist experience level through precedent research and made concept frame of study. The step of second checked up the effects of make-up artist experience through the desires for change. The step of third established to the effects of brand loyalty through make-up artist experience brand through mediation of make-up artist trust and brand satisfaction. The survey was carried out on 440 women aged 20 to 40 who experienced make-up services in Seoul, Gyeonggi-do, Sejong and Daejeon. The data were analyzed using SPSS 23.0, and AMOS 18.0 using frequency analysis, factor analysis, reliability analysis, structural model analysis and t-tests. 1)Make-up artist experiences were divided into informational/ relational experiences and emotional experiences. 2)Desires for change influenced positive effects about information/relational experience and emotional experience. 3)The information/relation experiences influenced positive effects about artist trust and brand satisfaction. 4)The emotional experiences influenced positive effects about make-up artist trust and brand satisfaction. 5) The make-up artist trust influenced positive effects about brand satisfaction. Trust in the make-up artist did not directly influence brand loyalty, but influenced it through satisfaction. 6)The brand satisfaction influenced positive effects about brand loyalty. This study identified the roles of make-up artist and the importance of the make-up experience.

• Steel and Composite Structures
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• 제29권5호
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• pp.603-622
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• 2018

Precast concrete structures are erected from individual prefabricated components, which are assembled on-site using different types of connections. In the present design of these structures, beam-to-column connections are assumed pin jointed. Bolted billet beam to-column connections have been used in the precast concrete industry for many years. They have many advantages over other jointing methods in component production, quality control, transportation and assembly. However, there is currently limited information concerning their detailed structural behaviour under vertical loadings. The experimental work has involved the determination of moment-relative rotation relationships for semi-rigid precast concrete connections in full-scale connection tests. The study reported in this paper was undertaken to clarify the behaviour of such connections under symmetrical vertical loadings. A series of full-scale tests was performed on sample column for which the column geometry and bolt arrangements conformed to successful commercial practice. Proprietary hollow core floor slabs were tied to the beams by 2T25 tensile reinforcing bars, which also provide the in-plane continuity across the connections. The contribution of the floor strength and stiffness to the flexural capacity of the joint is currently neglected in the design process for precast concrete frames. The flexural strength of the connections in the double-sided tests was at least 0.93 times the predicted moment of resistance of the composite beam and slab. The secant stiffness of the connections ranged from 0.94 to 1.94 times the flexural stiffness of the attached beam. In general, the double-sided connections were found to be more suited to a semi-rigid design approach than the single sided ones. The behaviour of double sided bolted billet connection test results are presented in this paper. The behaviour of single sided bolted billet connection test results is the subject of another paper.