• Journal of Korean Biological Nursing Science
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• v.24 no.4
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• pp.235-242
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• 2022

Purpose: To investigate associations of perceived stress level, serum cortisol level, and telomere length of community-dwelling adults in Korea. Methods: Data of a total of 135 community-dwelling adults aged over 40 years living in D metropolitan city from December 2020 to March 2021 were collected. Perceived stress level over the past month were measured using the Perceived Stress Score. Serum cortisol level was analyzed using a chemiluminescent microparticle immunoassay. Telomere length was determined using quantitative real-time polymerase chain reaction. The statistical package SPSS 23.0 was used to perform Chi-square test, independent t-test, and Pearson's correlation coefficient analysis. Results: There was no association between perceived stress and serum cortisol level (r = .07, p= .402). Serum cortisol level was not significantly associated with telomere length either (r = -.15, p= .081). However, the higher the perceived stress level, the shorter the telomere length (r= -.29, p= .001). Conclusion: These results suggest that perceived stress might induce physiological stress, which might partially affect gene biology. Further longitudinal research is needed to investigate the effect of perceived stress on telomere length. Intervention for relieving stress should be included in stabilizing the genetic environment of adults.

• Asian-Australasian Journal of Animal Sciences
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• v.32 no.8
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• pp.1095-1103
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• 2019

Objective: Among stress responses, the unfolded protein response (UPR) is a well-known mechanism related to endoplasmic reticulum (ER) stress. ER stress is induced by a variety of external and environmental factors such as starvation, ischemia, hypoxia, oxidative stress, and heat stress. Inositol requiring enzyme $1{\alpha}$ ($IRE1{\alpha}$)-X-box protein 1 (XBP1) is the most conserved pathway involved in the UPR and is the main component that mediates $IRE1{\alpha}$ signalling to downstream ER-associated degradation (ERAD)- or UPR-related genes. XBP1 is a transcription factor synthesised via a novel mechanism called 'frame switch splicing', and this process has not yet been studied in the horse XBP1 gene. Therefore, the aim of this study was to confirm the frame switch splicing of horse XBP1 and characterise its dynamics using Thoroughbred muscle cells exposed to heat stress. Methods: Primary horse muscle cells were used to investigate heat stress-induced frame switch splicing of horse XBP1. Frame switch splicing was confirmed by sequencing analysis. XBP1 amino acid sequences and promoter sequences of various species were aligned to confirm the sequence homology and to find conserved cis-acting elements, respectively. The expression of the potential XBP1 downstream genes were analysed by quantitative real-time polymerase chain reaction. Results: We confirmed that splicing of horse XBP1 mRNA was affected by the duration of thermal stress. Twenty-six nucleotides in the mRNA of XBP1 were deleted after heat stress. The protein sequence and the cis-regulatory elements on the promoter of horse XBP1 are highly conserved among the mammals. Induction of putative downstream genes of horse XBP1 was dependent on the duration of heat stress. We confirmed that both the mechanisms of XBP1 frame switch splicing and various binding elements found in downstream gene promoters are highly evolutionarily conserved. Conclusion: The frame switch splicing of horse XBP1 and its dynamics were highly conserved among species. These results facilitate studies of ER-stress in horse.

• Design & Manufacturing
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• v.8 no.2
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• pp.46-49
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• 2014

Silicone is recently used for LED chip lense due to its good thermal stability and optical transmittance. In order to predict residual stress which causes optical briefringence and mechanical warpage of silicone, finite element analysis was conducted for curing process during silicone molding. For analysis of curing process, a dynamic cure kinetics model was derived based on the differential scanning calorimetry(DSC) test and applied to the material properties for finite element analysis. Finite element simulation result showed that the slow cure reduced abrupt reaction heat and it was predicted decrease of the residual stress.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2000.11a
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• pp.194-200
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• 2000

Researches on the FRF-based substructuring method have been mainly focused on vibratory response analysis. Present study is concerned about the application of the method to the dynamic stress analysis of a compressor mounting bracket in a passenger car. This is performed by using reaction forces that can be obtained by the FRF-based substructuring method. The air-conditioner system, composed of a compressor, a bracket and a test jig, is analyzed by using the FRF-based substructuring method. The experimental and numerical FRFs are combined to calculate the system responses and reaction forces at the connection point. The dynamic reaction forces plugged into the bracket FE model to compute the compute the stresses of the bracket. Dynamic stresses by the present method are compared with those from FE model. The comparison shows possibility of practical usage of the method for the real problem.

• Animal Bioscience
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• v.34 no.6
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• pp.1006-1013
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• 2021

Objective: Cells have increased susceptibility to activation of apoptosis when suffering heat stress (HS). An effective supplementation strategy to mimic heat-induced apoptosis of bovine mammary epithelial cells (MECs) is necessary to maintain optimal milk production. This study aimed to investigate possible protective effects of the anti-apoptotic activity of 5-aminolevulinic acid (5-ALA) against HS-induced damage of bovine MECs. Methods: Bovine MECs were pretreated with or without 5-ALA at concentrations of 10, 100, and 500 µM for 24 h followed by HS (42.5℃ for 24 h and 48 h). Cell viability was measured with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays. Real-time quantitative polymerase chain reaction and Western blotting were used to explore the regulation of genes associated with apoptosis, oxidative stress, and endoplasmic reticulum (ER) stress genes. Results: We found that 5-ALA induces cytoprotection via inhibition of apoptosis markers after HS-induced damage. Pretreatment of bovine MECs with 5-ALA resulted in dramatic upregulation of mRNA for nuclear factor erythroid-derived 2-like factor 2, heme oxygenase-1, and NAD(P)H quinone oxidoreductase 1, all of which are antioxidant stress genes. Moreover, 5-ALA pretreatment significantly suppressed HS-induced ER stress-associated markers, glucose-regulated protein 78, and C/EBP homologous protein expression levels. Conclusion: 5-ALA can ameliorate the ER stress in heat stressed bovine MEC via enhancing the expression of antioxidant gene.

• Proceedings of the Computational Structural Engineering Institute Conference
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• 2000.04b
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• pp.141-149
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• 2000

This study performs the elastic and viscoelastic analysis of composite continuous beams with flexible shear connectors. Due to creep and shrinkage of the concrete part, the stress redistribution between the concrete slab and steel beam, and the evolution of the redundant restraint reaction occur with time. Using the equation of equilibrium, internal and external compatibility condition, and constitutive relationships, mathematical formulations are formulated. The solution is obtained by means of numerical step-by-step techniques and the finite difference method. Numerical parametric studies are performed to evaluate the stress redistribution, and the evolution of the redundant restraint reaction. The parameters include the stiffness and spacing of shear connectors, the age of concrete at loading, and the relative humidity.

• Korean Journal of Materials Research
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• v.9 no.4
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• pp.419-427
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• 1999

TiNi shape memory alloy was shape memory heat-treated and investigated its mechanical properties with the variation of prestrain. Also 6061 Al matrix composites with TiNi shape memory alloy fiber as reinforcement have been fabricated by Permanent Mold Casting to investigate the microstructures and interface properties. Yield stress of TiNi wire was the most high in the case of before heat-treatment and then decreased as increasing heat-treatment time. In each heat-treatment condition, the yield stress of TiNi wire was not changed with increasing the amount of prestrain. The interface bonding of TiNi/6061Al composite was fine. There was a 2$\mu\textrm{m}$ thickness of diffusion reaction layer at the interface. We could find out that this diffusion reaction layer was made by the mutual diffusion. The diffusion rate from Al base to TiNi wire was faster than that of reverse diffusion and the amount of the diffusion was also a little more than that of reverse.

• Journal of Welding and Joining
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• v.32 no.5
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• pp.13-20
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• 2014

A brief overview is given of the corrosion and hydrogen diffusion behaviors of high strength pipe steel in sour environment. Firstly, hydrogen adsorption and diffusion mechanism of the pipe steel is introduced. Secondly, the effect of iron sulfide film precipitated as a result of the corrosion reaction on the steel surface on hydrogen reduction reaction and subsequent hydrogen permeation through the steel is discussed. Moreover, the hydrogen diffusion behavior of the pipe steel under tensile stress in both elastic and plastic ranges is reviewed based on a number of experimental permeation data and theoretical models describing the hydrogen diffusion and trapping phenomena in the steel. It is hoped that this paper will result in significant academic contributions in the field of corrosion and hydrogen related problems of the pipe steel used in sour environment.

• Journal of the Microelectronics and Packaging Society
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• v.19 no.1
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• pp.67-73
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• 2012

After flash memory card(FMC) was manufactured by $B^2it$ process, interfacial reaction of silver bump with thermal stress was studied. To investigate bonding reliability of Ag bump, thermal shock and thermal stress tests were conducted and then examined on the crack between Cu land and Ag bump interface. Diffusion reaction of Ag bump/Cu land interface was analyzed using SEM, EDS and FIB. The Ag-Cu alloy layer due to the interfacial reaction was formed at the Ag/Cu interface. As the diffusivity of Ag ${\rightarrow}$ Cu is faster than Cu ${\rightarrow}$ Ag, a lot of (Cu, Ag) alloy layers were observed at the Cu layer than Ag. These alloy layers contributed to increase the Cu-Ag bonding strength and its reliability.

• The Journal of Korean Academic Society of Nursing Education
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• v.18 no.1
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• pp.149-158
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• 2012

Purpose: The purpose of this study was to identify the factors influencing mental health among late elementary age children. Methods: The research design was a secondary data analysis. Data were collected from 746 students in grades 5 or 6, and analyzed using descriptive statistics, t-test, ANOVA, Pearson's correlation coefficients and stepwise multiple regression with the SPSS/PC 18.0 program. Results: The average score of mental health was $3.42{\pm}0.55$. There were significant differences in mental health according to health status, economic status, scholastic performance, relationships with friends, relationships with the teachers, atmosphere of the home, number of siblings and the source of trouble. Stepwise multiple regression analysis showed influencing factors of stress, negative reaction under stress, self-esteem, teacher support, and friend support support and explained 41.4% of total variance in late elementary age children's mental health. Conclusion: These results may contribute to a better understanding of mental health in late elementary age children. The results of the present study indicate a need to develop nursing interventions to prevent and manage elementary school-age children's negative reaction under stress.