• 한국식물생명공학회:학술대회논문집
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• 한국식물생명공학회 2004년도 생명공학 실용화를 위한 비젼
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• pp.102-102
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• 2004

• Biomolecules & Therapeutics
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• 제32권3호
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• pp.349-360
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• 2024

Oxidative stress contributes to the onset of chronic diseases in various organs, including muscles. Morroniside, a type of iridoid glycoside contained in Cornus officinalis, is reported to have advantages as a natural compound that prevents various diseases. However, the question of whether this phytochemical exerts any inhibitory effect against oxidative stress in muscle cells has not been well reported. Therefore, the current study aimed to evaluate whether morroniside can protect against oxidative damage induced by hydrogen peroxide (H₂O₂) in murine C2C12 myoblasts. Our results demonstrate that morroniside pretreatment was able to inhibit cytotoxicity while suppressing H₂O₂-induced DNA damage and apoptosis. Morroniside also significantly improved the antioxidant capacity in H₂O₂-challenged C2C12 cells by blocking the production of cellular reactive oxygen species and mitochondrial superoxide and increasing glutathione production. In addition, H₂O₂-induced mitochondrial damage and endoplasmic reticulum (ER) stress were effectively attenuated by morroniside pretreatment, inhibiting cytoplasmic leakage of cytochrome c and expression of ER stress-related proteins. Furthermore, morroniside neutralized H₂O₂-mediated calcium (Ca²⁺) overload in mitochondria and mitigated the expression of calpains, cytosolic Ca²⁺-dependent proteases. Collectively, these findings demonstrate that morroniside protected against mitochondrial impairment and Ca²⁺-mediated ER stress by minimizing oxidative stress, thereby inhibiting H₂O₂-induced cytotoxicity in C2C12 myoblasts.

• 디지털융복합연구
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• 제14권11호
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• pp.527-537
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• 2016

본 연구는 간호대학생의 대인관계 조화, 스트레스 대처방식이 대학생활 적응에 미치는 영향을 파악하기 위한 서술적 조사연구이다. 대상자는 경북지역 2개 대학 간호학과 1, 2학년 재학생 231명이며, 2015년 12월 1일~11까지 설문조사 하였고, 기술통계, t-test, ANOVA, Pearson correlation coefficients, multiple regression으로 분석하였다. 대학생활 적응은 대인관계 조화 및 스트레스 대처방식과 유의한 상관관계를 보였다. 간호대학생의 대학생활 적응은 일반적 특성 중 학년, 간호학 지원동기, 간호학 전공만족도 및 학업성적에 따라 유의한 차이가 있었다. 대학생활 적응에 유의한 영향을 미치는 변수는 대인관계 조화(${\beta}=.27$, p<.001), 문제중심 대처(${\beta}=.25$, p<.001), 정서적 대처(${\beta}=-.25$, p<.001), 소망적 사고 대처(${\beta}=-.20$, p<.001)로 나타났고, 이들 변수의 대학생활 적응에 대한 설명력은 23%이었다. 결론적으로, 적절한 스트레스 대처방식과 대인관계 조화능력을 증진시키는 것은 간호대학생의 대학생활 적응을 향상시키는데 효과적인 전략이 될 수 있다.

• 지역사회간호학회지
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• 제12권3호
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• pp.696-704
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• 2001

Purpose: This study was to investigate the family life events stress and family functioning and social support of wives, and to find out the correlation among these variables. Method: The subjects were 263 wives who participated voluntarily in the questionnaire. The instrument used in this study comprised family life events stress, family functioning and social support instruments, and the data were collected from Sep.10 to Sep. 29 with self-administered questionnaires. We analyzed the data using descriptive statistics, Pearson correlation, t-test and ANOVA with SPSS win. Results: 1) The mean score of family life events stress was 25.8, family functioning was 47.2, and social support was 43.4(4 point scale), 2) The rate of correlation between family life events stress and family functioning was (r=-.18), that between family life events stress and social support was (r=-.26), and that between family functioning and social support was (r=0.46). 3) The mean score of family life events stress by general characteristics was significant in age, religion and family type. The mean score of family functioning by general characteristics was significant in education. The mean score of social support by general characteristics was not significant. Conclusion: Considering the results above, we can recognize that there are close relations among family life events stress, family functioning and social support. Although the correlation coefficient is somewhat different, these three variables are very significant for wives.

• 한국식물생명공학회:학술대회논문집
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• 한국식물생명공학회 2005년도 추계학술대회 및 한일 식물생명공학 심포지엄
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• pp.71-80
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• 2005

Effects of low temperature ($8^{\circ}C$) on the hydraulic conductivity of young roots of a chilling-sensitive (cucumber; Cucumis sativus L.) and a chilling-resistant (figleaf gourd; Cucurbita ficifolia Bouche) crop have been measured at the levels of whole root systems (root hydraulic conductivity, $Lp_r$) and of individual cortical cells (cell hydraulic conductivity, Lp). In figleaf gourd, there was a reduction only in hydrostatic $Lp_r$ but not in osmotic $Lp_r$ suggesting that the activity of water channels was not much affected by low root temperature (LRT)treatment in this species. Changes in cell Lp in response to chilling and recovery were similar asroot level, although they were more intense at the root level. Roots of figleaf gourd recovered better from LRT treatment than those of cucumber. In figleaf gourd, recovery (both at the root and cell level) often resulted in Lp and $Lp_r$ values which were even bigger than the original, i.e. there was an overshoot in hydraulic conductivity. These effects were larger forosmotic (representing the cell-to-cell passage of water) than for hydrostatic $Lp_r$. After a short term (1 d) exposure to $8\;^{\circ}C$ followed by 1 d at $20\;^{\circ}C$, hydrostatic $Lp_r$ of cucumber nearly recovered and that of figleaf gourd still remained higher due to the overshoot. On the contrary, osmotic $Lp_r$ and cell Lp in both species remained high by a factor of 3 as compared to the control, possibly due to an increased activity of water channels. After pre-conditioning of roots at LRT, increased hydraulic conductivitywas completely inhibited by $HgCl_2$ at both the root and cell levels. Different from figleaf gourd, recovery from chilling was not complete in cucumber after longer exposure to LRT. It is concluded that at LRT, both changes in the activity of aquaporins and alterations of root anatomy determine the water uptake in both species. To better understand the aquaporin function in plants under various stress conditions, we examined the transgenic Arabidopsisand tobacco plants that constitutively overexpress ArabidopsisPIP1;4 or PIP2;5 under various abiotic stress conditions. No significant differences in growth rates were found between the transgenic and wild-type plants under favorable growth conditions. By contrast, overexpression of PIP1;4 or PIP2;5 had a negative effect on seed germination and seedling growth under drought stress, whereas it had a positive effect under cold stress and no effect under salt stress. Measurement of water transport by cell pressure probe revealed that these observed phenotypes under different stress conditions were closely correlated with the ability of water transport by each aquaporin in the transgenic plants. Together, our results demonstrate that PIP-type aquaporins play roles in seed germination, seedling growth, and stress response of Arabidopsis and tobacco plants under various stress conditions, and emphasize the importance of a single aquaporin-mediated water transport in these cellular processes.

• 한국잠사학회:학술대회논문집
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• 한국잠사학회 2005년도 제48회 추계 학술연구 발표회
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• pp.32-32
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• 2005

• 한국산학기술학회논문지
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• 제21권4호
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• pp.100-108
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• 2020

본 연구는 간호대학생의 사회성, 생활스트레스, 우울이 스마트폰 중독에 미치는 영향을 살펴보고자 실시하였다. 연구대상자는 일부 지역 간호대학생을 대상으로 하였고, 자료수집기간은 2019년 10월부터 11월까지이며, 분석대상은 270명 이었다. 자료 분석은 일반적 특성에 따른 스마트폰 중독의 차이를 알아보기 위하여 빈도, 백분율, t-test, ANOVA 분석을 하였고, 스마트폰 중독에 미치는 영향요인을 Multiple regression으로 분석 하였다. 연구 결과 스마트폰 중독에 영향을 미치는 요인은 스마트폰 사용시간(β=.183, p<.001), 생활스트레스(β=.369, p<.001), 우울(β=.205, p=.012)이 스마트폰 중독에 유의한 영향을 미치며 설명력은 33.5%이었다. 또한 스마트폰 중독과의 상관관계에서는 스마트폰 중독과 생활스트레스(r=.50, p<.001), 우울(r=.35, p<.001)은 스마트폰 중독과 높은 양의 상관관계를 보였고, 스마트폰 중독과 사회성(r=-.20, p<.001), 사회성과 생활스트레스(r=-.28, p<.001)는 음의 상관관계로 나타났다. 결론적으로, 간호대학생의 스마트폰 중독에 스마트폰 사용시간, 생활스트레스, 우울이 중요한 영향을 미치는 요인으로 나타났으며, 간호 대학생의 스마트폰 중독을 예방하기 위해서는 스스로 스마트폰 사용시간 관리능력을 키우고, 생활스트레스와 우울을 감소시킬 수 있는 프로그램 개발을 강화하는 것이 필요하다고 하겠다.

• 문화기술의 융합
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• 제9권6호
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• pp.435-442
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• 2023

본 연구는 간호대학생의 우울, 자기효능감, 생활스트레스와 스마트폰 중독의 정도를 확인하고 이들의 상관관계 및 영향요인을 파악하기 위한 서술적 조사연구이다. 연구대상자의 우울 15.85점, 자기효능감 3.19점, 생활스트레스 2.47점, 스마트폰 중독 39.23점으로 나타났다. 일반적 특성에 따른 스마트폰 중독을 분석한 결과는 연령에서는 통계적으로 유의한 차이가 없는 것으로 나타났으며, 성별(t=-2.540, p=.014), 학년(F=3.080, p=.029), 스마트폰 중요도(F=16.192, p<.001), 스마트폰 사용시간(F=6.938, p<.001)에서는 유의한 차이가 있었다. 대상자의 스마트폰 중독은 우울(r=.351, p<0.01), 생활스트레스(r=.321, p<.001)와는 정적상관관계가 나타났으며, 자기효능감,(r=-.383, p<.001)은 부적 상관관계가 나타났다. 우울과 자기효능감(r=-.529, p<.001)은 부적 상관관계가 나타났으며, 생활스트레스(r=.568, p<.001)은 정적 상관관계가 나타났다. 자기효능감과 생활스트레스는(r=-.434, p<.001)부적 상관관계가 나타났다. 대상자의 스마트폰 중독의 영향요인으로는 자기효능감이 나타났다. 대학생의 자기효능감을 향상시키기 위한 프로그램 및 교육의 기초자료를 제공하고자 한다.

• 한국해양생명과학회지
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• 제6권1호
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• pp.31-37
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• 2021

Larvae from the marine medaka fish Oryzias javanicus were exposed with polystyrene microplastics (MPs) for 24 h. Exposure to waterborne fluorescent MPs showed clear ingestion and egestion in feces. Under constant MPs, the concentration of dissolved oxygen significantly decreased in 24 h compared to the control. Significant intracellular reactive oxygen species and malondialdehyde contents were detected in larvae, indicating oxidative stress and lipid peroxidation. Significant elevations in mRNA expressions of heat shock protein 70 and antioxidant defense system genes (glutathione reductase, glutathione peroxidase, catalase, and superoxide dismutase) were measured with increases in enzymatic activity of oxidative stress-related proteins. Taken together, the alterations to the molecular and biochemical components suggested that waterborne MPs had an oxidative stress effect on marine medaka larvae.

• 여성건강간호학회지
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• 제16권1호
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• pp.10-19
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• 2010

Purpose: The purpose of this study was to investigate the relationship among family support, stress and quality of life according to the phases of illness in breast cancer patients. Methods: A descriptive correlational study was employed with 121 breast cancer patients. The data was collected by using self reported questionnaire. Self reported data was collected by using the Family support scale, Perceived Stress Scale (PSS), and EORTC QLQ-BR23. Phases of illness consisted 1st phase, 2nd phase, 3rd phase. Results: The score of family supporting, stress and quality of life showed a statically differences according to the phase of illness. Family supporting and stress had negative relation in the first, second and third phase. Family supporting and quality of life in function area had positive relation in the first, second phase. There was no relation between family supporting and quality of life in symptom area. Stress, quality of life in symptom area and quality of life in function area had correlation in the first, third phase. Conclusion: This study suggest that the new nursing implementation should be considered according to the phase of illness in order to improve the family supporting and quality of life and reduce the stress in breast cancer patients through this study results.