• Journal of Oral Medicine and Pain
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• 제36권1호
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• pp.21-24
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• 2011

구강건조증이란 주관적인 구강 내 건조감으로 정의되며, 이는 약물, 타액선 질환, 방사선 치료, 쉐그렌 증후군, 심리적 요인 등과 같은 다양한 원인에서 비롯된다. 구강 건조증 환자 중 현저한 타액선 기능 감소가 존재하는 경우 구강캔디다증, 치아우식증, 치주질환, 미각변화, 구취 등의 병발증이 나타날 수 있다. 이러한 구강건조증의 치료로는 우선적으로 구강건조를 유발하는 원인요소를 제거하거나, 환자의 불편감을 감소시키기 위한 대증요법이 주가 되며 실제로 타액 분비 기능이 감소된 경우 이로 인한 합병증을 예방하기 위한 치료와 타액분비를 자극할 수 있는 약물치료를 시행할 수 있다. 이 중 타액분비를 촉진시키는 약물인 필로카핀은 구강건조증 치료제로 널리 사용되어 왔다. 하지만 발한작용, 비뇨기 및 위장관계의 비정상적인 기능유도, 심혈관계 및 호흡기계에 대한 위험 등의 부작용이 있어 천식, 만성폐질환, 심혈관계 질환자에게는 주의 깊은 사용이 요구되며, 특히 조절되지 않는 천식환자의 경우 필로카핀의 절대적 금기증으로 사용이 금지된다. 이처럼 구강건조증 치료에 있어 필로카핀은 부작용으로 인해 전신적인 투여에 많은 제한이 있다. 따라서, 필로카핀의 부작용을 최소화하기 위해 국소적으로 사용되는 방법 중의 하나인 필로카핀 양치액을 이용하여 치료한 증례를 통해 그 효과를 확인해보고자 하였다.

• BMB Reports
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• 제43권1호
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• pp.40-45
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• 2010

AMP-activated protein kinase (AMPK) is a heterotrimeric enzyme that plays a central role in cellular metabolic stress. Modulation of nitric oxide (NO) and cyclooxygenase-2 (COX-2) is considered a promising approach for the treatment of inflammation and neuronal diseases. In this study, the AMPK gene was fused in-frame with PEP-1 peptide in a bacterial expression vector to produce a PEP-1-AMPK fusion protein. Expressed and purified PEP-1-AMPK fusion proteins were transduced efficiently into macrophage Raw 264.7 cells in a time- and dose-dependent manner. Furthermore, transduced PEP-1-AMPK fusion protein markedly inhibited LPS-induced iNOS and COX-2 expression. These results suggest that the PEP-1-AMPK fusion protein can be used for the protein therapy of COX-2 and NO-related disorders such as inflammation and neuronal diseases.

• KSBB Journal
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• 제23권2호
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• pp.109-117
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• 2008

담자균류의 버섯은 대부분 영양생장단계의 균사체와 포자를 형성하는 생식생장단계인 자실체로 나누어진다. 그러나 자연산 상황버섯은 자연계에서의 분포수가 극히 적어 그 자실체를 얻기 어려우며, 이러한 희귀성으로 인해 매우 고가로 거래되고 있다. 1990년대 중반 상황버섯은 균사체 배양기술과 인공재배법이 확립된 후 농가에서 대량생산되기 시작하였으며, 이에 따라 상황버섯의 유효성분을 이용한 기능성 식품 및 의약품의 개발이 활발하였다. 그러나 인공재배법에 의한 상황버섯 추출물은 자연산 추출물의 항암 및 효능면에서 차이를 나타내고 있어, 자연산 자실체와 같은 수준에서의 약효를 나타내기 위한 인공배양기술과 고농도 유효성분을 획득하기 위한 추출법이 핵심기술로 대두되었고, 인공배양된 상황버섯과 자연산의 다각도적인 약효 비교에 관한 연구가 아직까지 진행되고 있다. 따라서 상황버섯의 유효성분이 높게 함유된 저렴한 추출물이 생산될 수 있는 기반 기술들이 기능성 음료용수, 식품 및 의약품으로 개발에 중요한 핵심기술로 떠오르고 있다. 국내 상황버섯 기능성 식품의 시장은 상황버섯의 효능에 대한 인지 증대, 웰빙열풍을 의한 건강 음료에 대한 선호도 증대 등으로 인해 향후에도 10% 이상의 높은 성장률을 보일 것으로 예상된다. 현재는 중소기업들이 주로 활동하고 있어 마케팅 및 유통의 한계로 인해 시장이 크게 확대되고 있지 않으나, 사업매력도가 긍정적으로 평가되고 있어 대기업에서도 상황버섯을 이용한 기능성 식품 시장에의 진출을 모색하고 있다.

• 한국콘텐츠학회논문지
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• 제11권3호
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• pp.327-338
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• 2011

본 연구는 자폐 장애로 진단받은 아동의 주양육자 총 52명을 대상으로 현재 받고 있는 언어치료 교육서비스에 대한 만족도와 욕구를 조사하기 위해 실시되었다. 연구방법은 설문조사 방식을 사용하였고, 설문 내용은 치료 교육 기관의 환경(7문항)과 치료사가 제공하는 프로그램(7문항), 그리고 치료 교육 만족도 및 서비스 욕구(7문항)를 포함한 세 가지 측면으로 구분하여 작성하였다. 연구 결과, 치료 교육 기관의 환경면에서, 치료비 등을 이유로 복지관 이용자의 만족도가 가장 높았으며, 사설 치료실, 종합병원 순으로 낮게 나타났다. 치료사가 제공하는 교육 프로그램 면에서, 실시하는 프로그램의 적절성 및 상담 내용 등을 이유로 종합병원 이용자의 만족도가 가장 높았으며, 사설치료실, 복지관 순으로 낮게 나타났다. 치료 교육 만족도 및 서비스 욕구 면에서, 언어치료 및 전체 치료 교육 서비스에 대한 만족도는 다소 만족하는 수준인 것으로 나타났다. 자녀가 언어치료를 받게 되고 난 후, 좋아진 점으로 일상적인 생활 기술의 향상과 심리적 안정이 높게 나타났으며, 추후 받고 싶은 치료 교육과 관련하여 개별, 집단 언어치료 교육 서비스에 대한 욕구가 매우 높게 나타났다. 이상의 결과를 통해, 치료 교육 기관에서 제공하는 서비스의 문제점을 파악하고, 자폐 아동의 치료 교육 만족도를 높이기 위해 언어치료사 및 관련 영역 전문가들이 노력해야 할 여러 가지 함의를 얻을 수 있었다.

• Journal of Preventive Medicine and Public Health
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• 제46권1호
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• pp.19-27
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• 2013

Objectives: This study was conducted to investigate the relationship between heat-related illnesses developed in the summer of 2012 and temperature. Methods: The study analyzed data generated by a heat wave surveillance system operated by the Korea Centers for Disease Control and Prevention during the summer of 2012. The daily maximum temperature, average temperature, and maximum heat index were compared to identify the most suitable index for this study. A piecewise linear model was used to identify the threshold temperature and the relative risk (RR) above the threshold temperature according to patient characteristics and region. Results: The total number of patients during the 3 months was 975. Of the three temperature indicators, the daily maximum temperature showed the best goodness of fit with the model. The RR of the total patient incidence was 1.691 (1.641 to 1.743) per $1^{\circ}C$ after $31.2^{\circ}C$. The RR above the threshold temperature of women (1.822, 1.716 to 1.934) was greater than that of men (1.643, 1.587 to 1.701). The threshold temperature was the lowest in the age group of 20 to 64 ($30.4^{\circ}C$), and the RR was the highest in the ${\geq}65$ age group (1.863, 1.755 to 1.978). The threshold temperature of the provinces ($30.5^{\circ}C$) was lower than that of the metropolitan cities ($32.2^{\circ}C$). Metropolitan cities at higher latitudes had a greater RR than other cities at lower latitudes. Conclusions: The influences of temperature on heat-related illnesses vary according to gender, age, and region. A surveillance system and public health program should reflect these factors in their implementation.

• 생명과학회지
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• 제30권2호
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• pp.191-201
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• 2020

사회의 급속한 발전과 함께 수반되는 환경오염이 인간의 건강을 위협하는 커다란 위험인자로 인식되기 시작하면서 공기오염을 억제하기 위한 노력과 공기오염에 의해 유발되는 여러 질환을 억제 및 치료하기 위한 연구개발이 급속히 증가하고 있다. 인간 건강에 나쁜 영향을 주는 공기오염의 주된 원인중의 하나인 미세먼지는 (particulate matter, PM) 크기에 따라 일반미세먼지와(PM₁₀) 초미세먼지(PM_2.5)로 나누어 질 수 있으며, 호흡기, 소화기, 및 피부에 흡수 및 부착되어 이상 면역반응을 유발하여 만성호흡기질환, 당뇨병 및 면역질환등을 촉진하는 것으로 알려져 있다. 그동안 인류의 건강을 위해 미세먼지의 발생을 억제하기 위한 범 국가적 노력과 함께 미세먼지의 유해성을 증명하기 위한 많은 연구가 진행되어 왔다. 본 총설에서는 여러 인체질환에 있어서 미세먼지가 미치는 유해성을 중심으로 소개하고 미세먼지의 생물학적 위험성을 평가하는 세포 및 동물실험법에 대해 요약하였다.

• Biomolecules & Therapeutics
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• 제26권2호
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• pp.146-156
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• 2018

Spermidine is a naturally occurring polyamine compound that has recently emerged with anti-aging properties and suppresses inflammation and oxidation. However, its mechanisms of action on anti-inflammatory and antioxidant effects have not been fully elucidated. In this study, the potential of spermidine for reducing pro-inflammatory and oxidative effects in lipopolysaccharide (LPS)-stimulated macrophages and zebrafish was explored. Our data indicate that spermidine significantly inhibited the production of pro-inflammatory mediators such as nitric oxide (NO) and prostaglandin $E_2$ ($PGE_2$), and cytokines including tumor necrosis $factor-{\alpha}$ and $interleukin-1{\beta}$ in RAW 264.7 macrophages without any significant cytotoxicity. The protective effects of spermidine accompanied by a marked suppression in their regulatory gene expression at the transcription levels. Spermidine also attenuated the nuclear translocation of $NF-{\kappa}B$ p65 subunit and reduced LPS-induced intracellular accumulation of reactive oxygen species (ROS) in RAW 264.7 macrophages. Moreover, spermidine prevented the LPS-induced NO production and ROS accumulation in zebrafish larvae and was found to be associated with a diminished recruitment of neutrophils and macrophages. Although more work is needed to fully understand the critical role of spermidine on the inhibition of inflammation-associated migration of immune cells, our findings clearly demonstrate that spermidine may be a potential therapeutic intervention for the treatment of inflammatory and oxidative disorders.

• 대한수의학회지
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• 제39권1호
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• pp.159-168
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• 1999

Swine whipworm(Trichuris suis) is cosmopolitan nematode which can cause serious pathology in immature stage(larva2~larva5) of infected pigs, such as anorexia, diarrhea, anemia, and death in heavy infections. In this larval stages, it is very difficult to diagnose the infection of whipworm and to differentiate from other common swine gastrointestinal disorders such as 21 day scours which are associated with TGE virus, rota virus, coccidium, and the stress of weaning. In this experiment, the isolated antigens of Trichuris spp. were carried out to examine the structure and specificity of antigens and to select the reasonable antigens which would be used in serological diagnosis by electrophoresis, Western blotting, ELISA. The results of this experiment were as follows; 1. The common fractions of each Trichuris suis antigen were identified 28,32,45, 80kDa by SDS-PAGE with silver stain and four major fractions could be detected in positive swine sera by Western blot analysis. 2. The OD(optical density) values of somatic and excretory-secretory antigens which were reacted against positive(negative) sera from pigs infected with Trichuris suis by ELISA reader were; 1) OD values($mean{\pm}SD$) of adult somatic antigen against positive(negative) sera were $0.30{\pm}0.12(0.09{\pm}0.006)$ and third-stage larva of somatic antigen were $0.28{\pm}0.038(0.10{\pm}0.005)$. And OD values of excretory-secretory antigens of adult and third-stage larva were $0.24{\pm}0.031(0.11{\pm}0.005)$ and $0.08{\pm}0.013(0.10{\pm}0.003)$, respectively. 2) OD values of adult somatic, larval somatic antigen and adult excretory-secretory antigen response to positive sera were significantly (p<0.01) associated with negative swine sera. And the Cut-off OD values(minimum positive value) were determined to be mean negative value plus 3 SD that would minimized the risk of false positives. 3. The OD values of somatic antigens of T suis and T vulpis against swine positive(negative) sera were $0.30{\pm}0.120(0.09{\pm}0.006)$ and $0.25{\pm}0.141(0.09{\pm}0.003)$. These data mean that the somatic antigens of T suis and T vulpis were able to diagnose T vulpis infection in dogs as well as T suis infection in pigs. These results suggest that somatic antigen of third-stage larva and excretory-secretory antigen of adult T suis could be used the diagnostic antigen by serological test(ELISA) in immature Trichuris spp. infection.

• 대한본초학회지
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• 제20권3호
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• pp.29-41
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• 2005

Objectives : The use of natural products with therapeutic properties is as ancient as human civilisation and, for a long time, mineral, plant and animal products were the main sources of drugs. Catalposide, the major iridoid glycoside isolated from the stem bark of Catalpa ovata G. Don (Bignoniceae) has been shown to possess anti-microbial and anti-tumoral properties. Heme oxygenase-1 (HO-1) is a stress response protein and is known to play a protective role against the oxidative injury. In this study, we examined whether catalposide could protect Neuro 2A cells, a kind of neuronal cell lines, from oxidative damage through the induction of HO-1 protein expression and HO activity. We also examined the effects of catalposide on the productions of tumor necrosis $factor-{\alpha}\;(TNF-{\alpha})$ and nitric oxide (NO) on RAW 264.7 macrophages activated with the endotoxin lipopolysaccharide. Methods : HO-1 expression in Neuro 2A cells was measured by Western blotting analysis. NO and $TNF--{\alpha}$ produced by RAW 264.7 macrophage were measured by Griess reagent and enzyme-linked immunosorbent assay, respectively. Results : The treatment of the cells with catalposide resulted in dose- and time-dependent up-regulations of both HO-1 protein expression and HO activity. Catalposide protected the cells from hydrogen peroxide-induced cell death. The protective effect of catalposide on hydrogen peroxide-induced cell death was abrogated by zinc protoporphyrin IX, a HO inhibitor. Additional experiments revealed the involvement of CO in the cytoprotective effect of catalposide-induced HO-1. In addition, catalposide inhibited the productions of $TNF--{\alpha}$ and NO with significant decreases in mRNA levels of $TNF--{\alpha}$ and inducible NO synthase. Conclusions : Our results indicate that catalposide is a potent inducer of HO-1 and HO-1 induction is responsible for the catalposide-mediated cytoprotection against oxidative damage and that catalposide may have therapeutic potential in the control of inflammatory disorders.

• 한국안전학회지
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• 제25권6호
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• pp.169-179
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• 2010

The aim of this study was to investigate occupational and individual risk factors and working conditions in relation to musculoskeletal symptoms in street cleaners. Investigation was conducted through a survey of 395 male street cleaners employed by the government office in Seoul, Gyeonggi and Chung-Nam from July to August of 2009. The control group was comprised of 143 male drivers and security guards. Risk factors for musculoskeletal symptoms in street cleaners were investigated by multiple logistic regression analysis and also evaluated ergonomic risk factors by assessing working conditions of 4 street cleaners. As a result of symptom questionnaires, all of the prevalent rates of musculoskeletal symptoms in street cleaners had significantly higher results than those of the control group(p<0.05). On binary logistic regression analysis of musculoskeletal symptoms, street cleaners showed significant higher odds ratio as 18.84(95%CI: 6.56-54.12) in the arm/elbow, 10.49(95%CI: 4.29-25.65) in the hand/wrist compared to the control group. Both absence of rest breaks and exposure to ergonomic risk factors showed to be important internal risk factors of musculoskeletal symptoms among street cleaners. The exposure levels of QEC(Quick exposures checklist) in street cleaners were revealed to be higher on the shoulder/arm, wrist/hand, and neck than back, or from stress. The findings appear to show that street cleaners were high-risk group of work-related musculoskeletal disorders. Therefore street cleaners require a holistic interventional strategy, including adequate arrangement of rest breaks, improvement of working tools and control of individual risk factors such as obesity and smoking.