• Earthquakes and Structures
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• v.16 no.2
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• pp.221-234
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• 2019

Today, many important concrete face rockfill dams (CFRDs) have been built on the world, and some of these important structures are located on the strong seismic regions. In this reason, examination and monitoring of these water construction's seismic behaviour is very important for the safety and future of these dams. In this study, the nonlinear seismic behaviour of Ilısu CFR dam which was built in Turkey in 2017, is investigated for various reservoir water heights taking into account 1995 Kobe near-fault and far-fault ground motions. Three dimensional (3D) finite difference model of the dam is created using the FLAC3D software that is based on the finite difference method. The most suitable mesh range for the 3D model is chosen to achieve the realistic numerical results. Mohr-Coulomb nonlinear material model is used for the rockfill materials and foundation in the seismic analyses. Moreover, Drucker-Prager nonlinear material model is considered for the concrete slab to represent the nonlinearity of the concrete. The dam body, foundation and concrete slab constantly interact during the lifetime of the CFRDs. Therefore, the special interface elements are defined between the dam body-concrete slab and dam body-foundation due to represent the interaction condition in the 3D model. Free field boundary condition that was used rarely for the nonlinear seismic analyses, is considered for the lateral boundaries of the model. In addition, quiet artificial boundary condition that is special boundary condition for the rigid foundation in the earthquake analyses, is used for the bottom of the foundation. The hysteric damping coefficients are separately calculated for all of the materials. These special damping values is defined to the FLAC3D software using the special fish functions to capture the effects of the variation of the modulus and damping ratio with the dynamic shear-strain magnitude. Total 4 different reservoir water heights are taken into account in the seismic analyses. These water heights are empty reservoir, 50 m, 100 m and 130 m (full reservoir), respectively. In the nonlinear seismic analyses, near-fault and far-fault ground motions of 1995 Kobe earthquake are used. According to the numerical analyses, horizontal displacements, vertical displacements and principal stresses for 4 various reservoir water heights are evaluated in detail. Moreover, these results are compared for the near-fault and far-faults earthquakes. The nonlinear seismic analysis results indicate that as the reservoir height increases, the nonlinear seismic behaviour of the dam clearly changes. Each water height has different seismic effects on the earthquake behaviour of Ilısu CFR dam. In addition, it is obviously seen that near-fault earthquakes and far field earthquakes create different nonlinear seismic damages on the nonlinear earthquake behaviour of the dam.

• Journal of the Korean Recycled Construction Resources Institute
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• v.6 no.4
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• pp.311-318
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• 2018

When reinforced concrete structures are exposed to fire, their mechanical properties such as compressive strength, elasticity coefficient and rebar yield strength, are degraded. Therefore, the structure's damage assessment is essential in determining whether to dismantle or augment the structure after a fire. In this study, the confinement effect of lateral reinforcement of RC column according to the numbers of fire exposure face and stirrup was verified by fire resistant test with the heating temperatures of $400^{\circ}C$, $600^{\circ}C$ and $800^{\circ}C$. The test results showed that the peak stress decreases and peak strain increases as the temperature is getting higher, also transverse ties are helpful in improving the compressive resistance of concrete subjected to high temperature. Based on the results of this study, the residual stress of confined concrete under thermal damage is higher at the condition of more lateral reinforcement ratio and less fire exposure faces. The decreasing ratio of elastic modulus of more confined and less exposure faces from the relationship of load and displacement was also smaller than that of opposite conditions.

• Korean Journal of Microbiology
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• v.55 no.3
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• pp.296-299
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• 2019

The genus of Bacteroides has been isolated from vertebrate animal feces. Bacteroides sp. KGMB 02408 was isolated from fecal samples obtained from a healthy Korean. The wholegenome sequence of Bacteroides sp. KGMB 02408 was analyzed using the PacBio Sequel platform. The genome comprises a 5,771,427 bp chromosome with a G + C content of 39.50%, 5,005 total genes, 18 rRNA genes, and 74 tRNA genes. Furthermore, we found that strain KGMB 02408 had some genes for oxidoreductases and menaquinone biosynthesis in its genome based on the result of genome analysis.

• Research in Plant Disease
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• v.25 no.1
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• pp.16-21
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• 2019

Botrytis cinerea is the pathogen for a gray mold generating problems during the cultivation and transportation of roses. But there is little information about the difference of the symptom severity caused by gray mold on rose varieties and pathogen strains. 16 strains were collected from the rose cultivation area to confirm the degree of disease occurrence against strains and each variety. Collected 16 strains were identified based on the sequences analysis of ITS region of ribosomal DNA by using specific primers. The sequence analysis was performed by comparing the sequences to find a difference. To confirm the difference in disease occurrence for each strains, the difference was classified from 0 to 5 stages using charmant variety as a control. The data was confirmed through Kruskal-Wallis ANOVA. The result showed the significant difference in the pathogenicity caused by strains. WNG6_5 showed the lowest pathogenicity with 0.24 and WNG6_3 showed the highest with 3.20. The difference between two strains were almost 3.0. In addition, nine varieties of roses were more investigated with three strains such as the strains of WNG6_5, Hwa_1, and WNG6_3. The result showed that the Love Letter variety showed resistance and the Ice Bear variety was sensitive to three strains. Taken together, this study showed the significant difference by the interactions of rose varieties and gray mold strains.

• Journal of Life Science
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• v.29 no.2
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• pp.248-255
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• 2019

This research confirmed the diversity and characterization of gut microorganisms isolated from the intestinal organs of Muraenesox cinereus, collected on the Samcheonpo Coast and Seocheon Coast in South Korea. To isolate strains, Marine agar medium was basically used and cultivated at $37^{\circ}C$ and pH7 for several days aerobically. After single colony isolation, totally 49 pure single-colonies were isolated and phylogenetic analysis was carried out based on the result of 16S rRNA gene DNA sequencing, indicating that isolated strains were divided into 3 phyla, 13 families, 15 genera, 34 species and 49 strains. Proteobacteria phylum, the main phyletic group, comprised 83.7% with 8 families, 8 genera and 26 species of Aeromonadaceae, Pseudoalteromonadaceae, Shewanellaceae, Enterobacteriaceae, Morganellaceae, Moraxellaceae, Pseudomonadaceae, and Vibrionaceae. To confirm whether isolated strain can produce industrially useful enzyme or not, amylase, lipase, and protease enzyme assays were performed individually, showing that 39 strains possessed at least one enzyme activity. Especially the Aeromonas sp. strains showed all enzyme activity tested. This result indicated that isolated strains have shown the possibility of the industrial application. Therefore, this study has contributed for securing domestic genetic resources and the expansion of scientific knowledge of the gut microbial community in Muraenesox cinereus of South Korea.

• Journal of Life Science
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• v.29 no.1
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• pp.84-89
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• 2019

Previous screening of novel antibacterial agents revealed that some bacterial isolates exhibited antibiotic activity against both gram-positive and gram-negative bacteria and that they showed antibacterial activity, even against methicillin-resistant Staphylococcus aureus (MRSA). Among these isolates, one bacterial strain, BCNU 1204, was identified as Pseudomonas aeruginosa using phenetic and phylogenetic analysis, based on 16S ribosomal RNA gene sequences. The maximum productivities of antimicrobial substances of BCNU 1204 were obtained after being cultured at $35^{\circ}C$ and pH 7.0 for 4 d in King's medium B (KMB). Dichloromethane (DCM) and ethylacetate (EA) extracts of P. aeruginosa BCNU 1204 exhibited strong antimicrobial activity, particularly against gram-positive bacteria. The EA extracts exhibited broad-spectrum activity against antibiotic resistant strains. Fraction 5-2, was obtained by recycling preparative liquid chromatography (LC) and preparative thin-layer chromatography (TLC) and was identified as phenazine-1-carboxylic acid belonging to phenazines using gas chromatography and mass spectrometry (GC/MS). Its minimum inhibitory concentration (MIC) values were $25{\mu}g/ml$, $50{\mu}g/ml$, ${\geq}25{\mu}g/ml$, and ${\geq}50{\mu}g/ml$ for MRSA CCARM 3089, 3090, 3091, and 3095 strains, respectively. P. aeruginosa BCNU 1204 may be a potential resource for the development of anti-MRSA antibiotics. Additional research is required to identify the active substance from P. aeruginosa BCNU 1204.

• Korean Journal of Clinical Laboratory Science
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• v.51 no.2
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• pp.164-170
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• 2019

The absorption and release capacities, survival efficiency, and recovery rates of five kinds of transport media were evaluated based on the swab elution method (Quantitative) of CLSI (Clinical and Laboratory Standards Institute) M40-A2. Liquid media showed mostly better results than semi-solid media in the three evaluations. The flocked swabs had better ability to absorption and discharge bacteria than the standard swabs. The liquid medium (S4) had the best survival efficiency. Pneumococcal strains with poor growth had a higher survival efficiency and recovery rate in liquid media (S4, S5). The results of microbial recovery showed that S. pyogenes met all the CLSI standards in all media. S. pneumoniae was inadequate in the semi- solid media (S2, S3) and all the remaining media met the criteria. H. influenzae was unsuitable in semi-solid media (S1, S3) and met the criteria in semi-solid medium (S2) and liquid medium (S4, S5). The viability of the H. influenzae, pneumococcal strain causing respiratory disease, was poor in most media. Overgrowth of P. aeruginosa was observed at room temperature. The combination of liquid medium and flocked swab confirmed the best results in the three evaluation methods.

• Journal of the Korean Geotechnical Society
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• v.35 no.7
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• pp.15-27
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• 2019

The purpose of this study is to investigate the mechanism about damages occurring at quay wall and backfill in Youngilman Port during Pohang earthquake (M5.4) on November 15, 2017. In the field investigation, the horizontal displacement of the caisson occurred between 5 cm and 15 cm, and the settlement at backfill occurred higher than 10 cm. 2D-effective Stress Analysis was performed to clarify the mechanism for the damage. The input earthquake motion used acceleration ($3.25m/s^2$) measured at bedrock of Pohang habor. Based on a numerical analysis, it was found that the effective stress decreased due to the increase of excess pore pressure in the backfill ground and the horizontal displacement of the caisson occurred by about 14 cm, and the settlement occurred by about 3 cm. In backfill, the settlements occurred between 6 cm and 9 cm. This is similar to field investigation results. Also, it was found that the backfill soil was close to the Mohr-Coulomb failure line due to the cyclic loading from the effective stress path and the stress-strain behavior. It may be related to decreasing of bearing capacity induced by the reduction of effective stress caused by the increase of the excess pore water pressure.

• Korean Journal of Microbiology
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• v.54 no.4
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• pp.456-459
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• 2018

The genus of Olsenella has been isolated from vertebrate animal mouth, rumen, and feces. Olsenella sp. KGMB 04489 was isolated from fecal samples obtained from a healthy Korean. The whole-genome sequence of Olsenella sp. KGMB 04489 was analyzed using the PacBio Sequel platform. The genome comprises a 2,108,034 bp chromosome with a G + C content of 65.50%, 1,838 total genes, 13 rRNA genes, and 52 tRNA genes. Also, we found that strain KGMB 04489 had some genes for hydrolysis enzymes, and antibiotic biosynthesis and resistance in its genome based on the result of genome analysis.

• Phonetics and Speech Sciences
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• v.12 no.4
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• pp.91-98
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• 2020

This study investigated the acoustic characteristics of sustained vowel /a/ and sentence utterance produced by patients with muscle tension dysphonia (MTD) using cepstrum-based acoustic variables. 36 women diagnosed with MTD and the same number of women with normal voice participated in the study and the data were recorded and measured by ADSVTM. The results demonstrated that cepstral peak prominence (CPP) and CPP_F0 among all of the variables were statistically significantly lower than those of control group. When it comes to the GRBAS scale, overall severity (G) was most prominent, and roughness (R), breathiness (B), and strain (S) indices followed in order in the voice quality of MTD patients. As these characteristics increased, a statistically significant negative correlation was observed in CPP. We tried to classify MTD and control group using CPP and CPP_F0 variables. As a result of statistic modeling with a Random Forest machine learning algorithm, much higher classification accuracy (100% in training data and 83.3% in test data) was found in the sentence reading task, with CPP being proved to be playing a more crucial role in both vowel and sentence reading tasks.